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WO2014122422A3 - Amplification technique - Google Patents

Amplification technique Download PDF

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Publication number
WO2014122422A3
WO2014122422A3 PCT/GB2014/050102 GB2014050102W WO2014122422A3 WO 2014122422 A3 WO2014122422 A3 WO 2014122422A3 GB 2014050102 W GB2014050102 W GB 2014050102W WO 2014122422 A3 WO2014122422 A3 WO 2014122422A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
nucleotides
amplification reaction
acid amplification
polymorphism
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2014/050102
Other languages
French (fr)
Other versions
WO2014122422A2 (en
Inventor
Robert Powell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Primerdesign Ltd
Original Assignee
Primerdesign Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB1302112.6A external-priority patent/GB201302112D0/en
Priority claimed from GB201308291A external-priority patent/GB201308291D0/en
Application filed by Primerdesign Ltd filed Critical Primerdesign Ltd
Publication of WO2014122422A2 publication Critical patent/WO2014122422A2/en
Publication of WO2014122422A3 publication Critical patent/WO2014122422A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for carrying out a nucleic acid amplification reaction to amplify a specific sequence which includes a polymorphism, which method comprising carrying out a nucleic acid amplification reaction using a nucleic acid amplification reaction mixture which comprises a nucleic acid primer having a 3'end that binds at or near the site of the polymorphism, and a set of nucleotides able to effect amplification of the target sequence and wherein the composition of the nucleotides is selected so as to enhance the specificity of the primer for the specific sequence. In particular, a substitute nucleotide or modified nucleotide analogue that competes with the natural nucleotide for incorporation is included in the reaction at a relatively high concentration, and/or the concentration of one or more of the nucleotides is reduced.
PCT/GB2014/050102 2013-02-06 2014-01-15 Amplification technique Ceased WO2014122422A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB1302112.6A GB201302112D0 (en) 2013-02-06 2013-02-06 Amplification technique
GB1302112.6 2013-02-06
GB1308291.2 2013-05-08
GB201308291A GB201308291D0 (en) 2013-05-08 2013-05-08 Amplification technique

Publications (2)

Publication Number Publication Date
WO2014122422A2 WO2014122422A2 (en) 2014-08-14
WO2014122422A3 true WO2014122422A3 (en) 2014-11-27

Family

ID=51210681

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2014/050102 Ceased WO2014122422A2 (en) 2013-02-06 2014-01-15 Amplification technique

Country Status (1)

Country Link
WO (1) WO2014122422A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104389026A (en) * 2014-10-30 2015-03-04 北京诺禾致源生物信息科技有限公司 Method for establishing single cell transcriptome sequencing library and application of method
TW201922138A (en) 2017-10-17 2019-06-16 英商Ｈｄ３有限公司 Hair styling device, hair styling method and drive system

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009151921A1 (en) * 2008-05-27 2009-12-17 Trilink Biotechnologies Chemically modified nucleoside 5'-triphosphates for thermally initiated amplification of nucleic acid

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009151921A1 (en) * 2008-05-27 2009-12-17 Trilink Biotechnologies Chemically modified nucleoside 5'-triphosphates for thermally initiated amplification of nucleic acid

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
"BRAF V600E Handbook", 15 January 2004, PRIMER DESIGN LTD, article "BRAF V600E Handbook", XP055143531 *
PASTINEN T ET AL: "A system for specific, high-throughput genotyping by allele-specific primer extension on microarrays", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, WOODBURY, NY, US, vol. 10, no. 7, 1 July 2000 (2000-07-01), pages 1031 - 1042, XP002228091, ISSN: 1088-9051, DOI: 10.1101/GR.10.7.1031 *

Also Published As

Publication number Publication date
WO2014122422A2 (en) 2014-08-14

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