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WO2019009592A3 - Ucst 소재를 적용한 입자 및 이를 이용한 핵산 증폭 방법 - Google Patents

Ucst 소재를 적용한 입자 및 이를 이용한 핵산 증폭 방법 Download PDF

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Publication number
WO2019009592A3
WO2019009592A3 PCT/KR2018/007523 KR2018007523W WO2019009592A3 WO 2019009592 A3 WO2019009592 A3 WO 2019009592A3 KR 2018007523 W KR2018007523 W KR 2018007523W WO 2019009592 A3 WO2019009592 A3 WO 2019009592A3
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WO
WIPO (PCT)
Prior art keywords
same
primer
particle
ucst
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2018/007523
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English (en)
French (fr)
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WO2019009592A2 (ko
Inventor
김상경
김준선
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Korea Institute of Science and Technology KIST
Original Assignee
Korea Institute of Science and Technology KIST
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020180076483A external-priority patent/KR102088877B1/ko
Application filed by Korea Institute of Science and Technology KIST filed Critical Korea Institute of Science and Technology KIST
Priority to US16/628,346 priority Critical patent/US20200239944A1/en
Priority to CN201880057430.3A priority patent/CN111356771A/zh
Publication of WO2019009592A2 publication Critical patent/WO2019009592A2/ko
Publication of WO2019009592A3 publication Critical patent/WO2019009592A3/ko
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 전방향 및 역방향 프라이머 중 하나 이상의 프라이머 및/또는 프로브를 UCST(Upper Critical Solution Temperature) 입자에 포함시켜 핵산 증폭시, 또는 상기 전방향 및 역방향 프라이머 중 하나 이상의 프라이머 및/또는 프로브를 UCST 입자에 포함시켜 하이드로젤 미세입자에 고정함으로써 핵산 증폭시 특정 온도범위에서 상기 UCST 입자에 포함된 프라이머 또는 프로브를 방출시킬 수 있다. 이로써 프라이머 이중체의 생성을 방지함과 동시에 우수한 PCR 증폭 효율을 나타낼 수 있다.
PCT/KR2018/007523 2017-07-04 2018-07-03 Ucst 소재를 적용한 입자 및 이를 이용한 핵산 증폭 방법 Ceased WO2019009592A2 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US16/628,346 US20200239944A1 (en) 2017-07-04 2018-07-03 Particle with ucst material applied thereto, and nucleic acid amplification method using same
CN201880057430.3A CN111356771A (zh) 2017-07-04 2018-07-03 适用ucst材料的粒子及利用其的核酸扩增方法

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20170084595 2017-07-04
KR10-2017-0084595 2017-07-04
KR1020180076483A KR102088877B1 (ko) 2017-07-04 2018-07-02 Ucst 소재를 적용한 입자 및 이를 이용한 핵산 증폭 방법
KR10-2018-0076483 2018-07-02

Publications (2)

Publication Number Publication Date
WO2019009592A2 WO2019009592A2 (ko) 2019-01-10
WO2019009592A3 true WO2019009592A3 (ko) 2019-04-11

Family

ID=64950195

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2018/007523 Ceased WO2019009592A2 (ko) 2017-07-04 2018-07-03 Ucst 소재를 적용한 입자 및 이를 이용한 핵산 증폭 방법

Country Status (1)

Country Link
WO (1) WO2019009592A2 (ko)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6642000B1 (en) * 1999-11-12 2003-11-04 University Of Chicago PCR amplification on microarrays of gel immobilized oligonucleotides
US20100081175A1 (en) * 2007-05-25 2010-04-01 Beckman Coulter, Inc. Enzyme-containing gels and nucleic acid amplifying kits
KR101670232B1 (ko) * 2013-10-28 2016-10-31 한국과학기술연구원 다공성 구조체 및 이의 제조방법
KR101746055B1 (ko) * 2016-05-03 2017-06-12 한국과학기술연구원 코어-쉘 구조의 다공성 구조체 및 이를 이용한 핵산 증폭 방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6642000B1 (en) * 1999-11-12 2003-11-04 University Of Chicago PCR amplification on microarrays of gel immobilized oligonucleotides
US20100081175A1 (en) * 2007-05-25 2010-04-01 Beckman Coulter, Inc. Enzyme-containing gels and nucleic acid amplifying kits
KR101670232B1 (ko) * 2013-10-28 2016-10-31 한국과학기술연구원 다공성 구조체 및 이의 제조방법
KR101746055B1 (ko) * 2016-05-03 2017-06-12 한국과학기술연구원 코어-쉘 구조의 다공성 구조체 및 이를 이용한 핵산 증폭 방법

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DROBYSHEV, ALEXEI L.: "The role of DNA diffusion in solid phase polymerase chain reaction with gel-immobilized primers in planar and capillary microarray format", BIOMICROFLUIDICS, vol. 3, no. 4, 2009, pages 1 - 12, XP055590092 *

Also Published As

Publication number Publication date
WO2019009592A2 (ko) 2019-01-10

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