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WO2018226964A3 - Promoteurs issus de corynebacterium glutamicum et leurs utilisations dans la régulation de l'expression génique ancillaire - Google Patents

Promoteurs issus de corynebacterium glutamicum et leurs utilisations dans la régulation de l'expression génique ancillaire Download PDF

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Publication number
WO2018226964A3
WO2018226964A3 PCT/US2018/036472 US2018036472W WO2018226964A3 WO 2018226964 A3 WO2018226964 A3 WO 2018226964A3 US 2018036472 W US2018036472 W US 2018036472W WO 2018226964 A3 WO2018226964 A3 WO 2018226964A3
Authority
WO
WIPO (PCT)
Prior art keywords
promoters
gene expression
corynebacterium glutamicum
regulating
host cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2018/036472
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English (en)
Other versions
WO2018226964A2 (fr
Inventor
Zach Serber
Katherine G. GORA
Shawn P. MANCHESTER
Peter ENYEART
Alexander SHEARER
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zymergen Inc
Original Assignee
Zymergen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zymergen Inc filed Critical Zymergen Inc
Priority to CN201880045247.1A priority Critical patent/CN110869504A/zh
Priority to KR1020207000506A priority patent/KR20200026881A/ko
Priority to JP2019567365A priority patent/JP2020524492A/ja
Priority to US16/620,188 priority patent/US20200239897A1/en
Priority to CA3064777A priority patent/CA3064777A1/fr
Priority to EP18740369.6A priority patent/EP3635117A2/fr
Publication of WO2018226964A2 publication Critical patent/WO2018226964A2/fr
Publication of WO2018226964A3 publication Critical patent/WO2018226964A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C12N2330/51—Specially adapted vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/55—Vector systems having a special element relevant for transcription from bacteria

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention concerne des promoteurs natifs comprenant des polynucléotides isolés de Corynebacterium glutamicum, ainsi que des promoteurs mutants qui sont dérivés de ces derniers et qui peuvent servir à réguler, c'est-à-dire augmenter ou réduire, l'expression génique dans la voie et/ou hors voie. L'invention concerne également des échelles de promoteurs comprenant une pluralité de promoteurs ayant une activité de promoteur augmentant progressivement. L'invention concerne en outre des cellules hôtes et des vecteurs recombinants comprenant lesdits promoteurs, ainsi que des procédés d'expression des gènes ancillaires d'intérêt et de production de biomolécules au moyen des cellules hôtes.
PCT/US2018/036472 2017-06-07 2018-06-07 Promoteurs issus de corynebacterium glutamicum et leurs utilisations dans la régulation de l'expression génique ancillaire Ceased WO2018226964A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CN201880045247.1A CN110869504A (zh) 2017-06-07 2018-06-07 来自谷氨酸棒状杆菌的启动子及其在调节辅助基因表达中的用途
KR1020207000506A KR20200026881A (ko) 2017-06-07 2018-06-07 코리네박테리움 글루타미컴으로부터의 프로모터 및 보조 유전자 발현을 조절하는 데 이의 용도
JP2019567365A JP2020524492A (ja) 2017-06-07 2018-06-07 Ｃｏｒｙｎｅｂａｃｔｅｒｉｕｍ ｇｌｕｔａｍｉｃｕｍ由来のプロモーターおよび補助遺伝子発現の制御におけるその使用
US16/620,188 US20200239897A1 (en) 2017-06-07 2018-06-07 Promoters from corynebacterium glutamicum and uses thereof in regulating ancillary gene expression
CA3064777A CA3064777A1 (fr) 2017-06-07 2018-06-07 Promoteurs issus de corynebacterium glutamicum et leurs utilisations dans la regulation de l'expression genique ancillaire
EP18740369.6A EP3635117A2 (fr) 2017-06-07 2018-06-07 Promoteurs issus de corynebacterium glutamicum et leurs utilisations dans la régulation de l'expression génique ancillaire

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762516609P 2017-06-07 2017-06-07
US62/516,609 2017-06-07

Publications (2)

Publication Number Publication Date
WO2018226964A2 WO2018226964A2 (fr) 2018-12-13
WO2018226964A3 true WO2018226964A3 (fr) 2019-05-09

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2018/036472 Ceased WO2018226964A2 (fr) 2017-06-07 2018-06-07 Promoteurs issus de corynebacterium glutamicum et leurs utilisations dans la régulation de l'expression génique ancillaire

Country Status (7)

Country Link
US (1) US20200239897A1 (fr)
EP (1) EP3635117A2 (fr)
JP (1) JP2020524492A (fr)
KR (1) KR20200026881A (fr)
CN (1) CN110869504A (fr)
CA (1) CA3064777A1 (fr)
WO (1) WO2018226964A2 (fr)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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WO2018005793A1 (fr) 2016-06-30 2018-01-04 Zymergen Inc. Procédés de production d'une banque de glucose perméase et utilisations associées
CN110885364B (zh) * 2019-12-26 2021-02-02 江南大学 一种促进N-乙酰氨基葡萄糖生产的RamA转录因子突变体及其应用
CN111197021B (zh) * 2020-01-13 2021-09-21 江南大学 一种l-赖氨酸产量提高的重组谷氨酸棒杆菌及其构建方法
CN111607601B (zh) * 2020-04-24 2022-10-18 天津大学 谷氨酸棒杆菌转录调控因子IpsA突变体及应用
KR102470602B1 (ko) * 2020-12-11 2022-11-25 씨제이제일제당 주식회사 신규한 분지 연쇄 아미노산 아미노트렌스퍼라아제 변이체 및 이를 이용한 이소류신 생산 방법
CN112592924B (zh) * 2020-12-28 2022-07-01 宁夏伊品生物科技股份有限公司 一种yh66_10325基因改造的产l-异亮氨酸重组菌株及其构建方法与应用
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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006028063A1 (fr) * 2004-09-09 2006-03-16 Research Institute Of Innovative Technology For The Earth Fragment d'adn ayant une fonction promotrice
US20090029356A1 (en) * 1999-07-08 2009-01-29 Basf Ag Corynebacterium glutamicum genes encoding novel proteins

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU507634A1 (ru) 1973-09-24 1976-03-25 Институт Микробиологии Имени А.Кирхенштейна Ан Латвийской Сср Способ получени -лизина
JPS5835197A (ja) 1981-08-26 1983-03-01 Kyowa Hakko Kogyo Co Ltd プラスミドｐｃｇ２
US4601983A (en) 1983-06-15 1986-07-22 Ajinomoto Co., Inc. Coryneform bacteria carrying recombinant plasmids and their use in the fermentative production of L-threonine and L-isoleucine
US4601893A (en) 1984-02-08 1986-07-22 Pfizer Inc. Laminate device for controlled and prolonged release of substances to an ambient environment and method of use
GB2165546B (en) 1984-08-21 1989-05-17 Asahi Chemical Ind A plasmid containing a gene for tetracycline resistance and dna fragments derived therefrom
JPH0655149B2 (ja) 1985-03-12 1994-07-27 協和醗酵工業株式会社 Ｌ―リジンの製造法
JP2748418B2 (ja) 1988-08-03 1998-05-06 味の素株式会社 組換えｄｎａ、該組換えｄｎａを有する微生物
US5976843A (en) 1992-04-22 1999-11-02 Ajinomoto Co., Inc. Bacterial strain of Escherichia coli BKIIM B-3996 as the producer of L-threonine
DE4027453A1 (de) 1990-08-30 1992-03-05 Degussa Neue plasmide aus corynebacterium glutamicum und davon abgeleitete plasmidvektoren
JP3023615B2 (ja) 1990-08-30 2000-03-21 協和醗酵工業株式会社 発酵法によるｌ―トリプトファンの製造法
WO1992006189A1 (fr) 1990-09-27 1992-04-16 Invitrogen Corporation Clonage direct d'acides nucleiques amplifies par reaction en chaine de la polymerase
DE4130867A1 (de) 1991-09-17 1993-03-18 Degussa Verfahren zur fermentativen herstellung von aminosaeuren
DE4440118C1 (de) 1994-11-11 1995-11-09 Forschungszentrum Juelich Gmbh Die Genexpression in coryneformen Bakterien regulierende DNA
MX9704236A (es) 1994-12-09 1998-01-31 Ajinomoto Kk Gen para la lisina descarboxilasa novedosa y metodo para producir la l-lisina.
AU706285B2 (en) 1995-05-16 1999-06-10 Ajinomoto Co., Inc. Feed additive
GB2304718B (en) 1995-09-05 2000-01-19 Degussa The production of tryptophan by the bacterium escherichia coli
DE19548222A1 (de) 1995-12-22 1997-06-26 Forschungszentrum Juelich Gmbh Verfahren zur mikrobiellen Herstellung von Aminosäuren durch gesteigerte Aktivität von Exportcarriern
JPH09224661A (ja) 1996-02-23 1997-09-02 Mitsubishi Chem Corp グルコース−６−リン酸デヒドロゲナーゼおよびそれをコードするｄｎａ
AU3938397A (en) * 1996-08-23 1998-03-06 Peter Ruhdal Jensen Artificial promoter libraries for selected organisms and promoters derived from such libraries
JPH10229891A (ja) 1997-02-20 1998-09-02 Mitsubishi Rayon Co Ltd マロン酸誘導体の製造法
DE19831609B4 (de) 1997-10-04 2009-11-12 Evonik Degussa Gmbh Verfahren zur Herstellung von Aminosäuren der Aspartat- und/oder Glutamatfamilie und im Verfahren einsetzbare Mittel
US5990350A (en) 1997-12-16 1999-11-23 Archer Midland Company Process for making granular L-lysine
WO2001000843A2 (fr) * 1999-06-25 2001-01-04 Basf Aktiengesellschaft Genes de corynebacterum glutamicum codant pour des proteines de voie metabolique
US6586214B1 (en) 1999-09-15 2003-07-01 Degussa Ag Method for increasing the metabolic flux through the pentose phosphate cycle in coryneform bacteria by regulation of the phosphoglucose isomerase (pgi gene)
DE19947791A1 (de) 1999-10-05 2001-04-12 Degussa Neue für das eno-Gen codierende Nukleotidsequenzen
DE19950409A1 (de) 1999-10-20 2001-04-26 Degussa Neue für das pck-Gen codierende Nukleotidsequenzen
DE19951975A1 (de) 1999-10-28 2001-05-03 Degussa Neue für das poxB-Gen codierende Nuleotidsequenzen
DE19959327A1 (de) 1999-12-09 2001-06-13 Degussa Neue für das zwa2-Gen codierende Nukleotidsequenzen
DE19959328A1 (de) 1999-12-09 2001-06-13 Degussa Neue für das zwa1-Gen codierende Nukleotidsequenzen
JP4623825B2 (ja) 1999-12-16 2011-02-02 協和発酵バイオ株式会社 新規ポリヌクレオチド
US7160711B2 (en) 2001-08-06 2007-01-09 Degussa Ag Coryneform bacteria which produce chemical compounds I
WO2003040373A2 (fr) 2001-08-06 2003-05-15 Degussa Ag Bacteries coryneformes generatrices de composes chimiques
CN100336901C (zh) 2001-08-06 2007-09-12 德古萨股份公司 生产化合物ⅱ的棒状细菌
JP2003219807A (ja) 2002-01-25 2003-08-05 Ajinomoto Co Inc Ｌ−リジンを主成分とする造粒乾燥物
US20040115304A1 (en) 2002-12-16 2004-06-17 Frank Dubner Feesdstuffs additives containing L-lysine with improved abrasion resistance, and process for their production
WO2005021772A1 (fr) 2003-08-29 2005-03-10 Degussa Ag Procede de preparation de l-lysine
DE102004061846A1 (de) 2004-12-22 2006-07-13 Basf Ag Mehrfachpromotoren
EP1907559A1 (fr) 2005-07-18 2008-04-09 Basf Se Micro-organismes recombinés producteurs de méthionine
KR100789274B1 (ko) * 2007-01-15 2008-01-02 씨제이 주식회사 코리네박테리움 글루타미쿰에서 유래한 신규한 프로모터핵산 분자, 그 프로모터를 포함하는 재조합 벡터, 그재조합 벡터를 포함하는 숙주 세포 및 그 숙주 세포를이용하여 유전자를 발현하는 방법
WO2009043372A1 (fr) 2007-10-02 2009-04-09 Metabolic Explorer Accroissement du rendement en méthionine
US20170145449A1 (en) * 2014-06-11 2017-05-25 Institut National De La Recherche Agronomique Improved lipid accumulation in Yarrowia lipolytica strains by overexpression of hexokinase and new strains thereof
KR20180084756A (ko) * 2015-12-07 2018-07-25 지머젠 인코포레이티드 코리네박테리움 글루타미컴으로부터의 프로모터
ES2856755T3 (es) * 2015-12-07 2021-09-28 Zymergen Inc Promotores de Corynebacterium glutamicum
WO2018005793A1 (fr) * 2016-06-30 2018-01-04 Zymergen Inc. Procédés de production d'une banque de glucose perméase et utilisations associées
JP2019519242A (ja) * 2016-06-30 2019-07-11 ザイマージェン インコーポレイテッド 細菌ヘモグロビンライブラリーを生成するための方法およびその使用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090029356A1 (en) * 1999-07-08 2009-01-29 Basf Ag Corynebacterium glutamicum genes encoding novel proteins
WO2006028063A1 (fr) * 2004-09-09 2006-03-16 Research Institute Of Innovative Technology For The Earth Fragment d'adn ayant une fonction promotrice

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [online] 20 September 2006 (2006-09-20), "DNA fragment having promoter function.", XP002767746, retrieved from EBI accession no. EM_PAT:DD324094 Database accession no. DD324094 *
DATABASE EMBL [online] 22 January 2006 (2006-01-22), "Corynebacterium glutamicum tatE gene for Sec-independent protein secretion pathway component, complete cds.", XP002787740, retrieved from EBI accession no. EM_STD:AB247377 Database accession no. AB247377 *
DATABASE Geneseq [online] 2 April 2009 (2009-04-02), "C. glutamicum marker and fine chemical production (MCP) DNA SEQ ID:2731.", XP002787739, retrieved from EBI accession no. GSN:AWF58557 Database accession no. AWF58557 *
DATABASE Geneseq [online] 8 March 2007 (2007-03-08), "Corynebacterium glutamicum DNA gyrase subunit B DNA, SEQ ID: 123.", XP002767747, retrieved from EBI accession no. GSN:AEM36105 Database accession no. AEM36105 *
DATABASE Geneseq [online] 8 March 2007 (2007-03-08), "Corynebacterium glutamicum DNA gyrase subunit B DNA, SEQ ID: 123.", XP002770467, retrieved from EBI accession no. GSN:AEM36105 Database accession no. AEM36105 *
JOSE-LUIS ADRIO ET AL: "Recombinant organisms for production of industrial products", BIOENGINEERED BUGS, vol. 1, no. 2, 1 March 2010 (2010-03-01), pages 116 - 131, XP055141219, ISSN: 1949-1018, DOI: 10.4161/bbug.1.2.10484 *
KIKUCHI YOSHIMI ET AL: "Functional analysis of the twin-arginine translocation pathway in Corynebacterium glutamicum ATCC 13869", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 72, no. 11, 1 November 2006 (2006-11-01), pages 7183 - 7192, XP002542576, ISSN: 0099-2240, DOI: 10.1128/AEM.01528-06 *
RASTEGARI H ET AL: "Improvement in the production of L-lysine by overexpression of aspartokinase (ASK) in C. glutamicum ATCC 21799", TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH 2013 PHARMACOTHERAPY GROUP NGA, vol. 12, no. 1, February 2013 (2013-02-01), pages 51 - 56, XP002767748, ISSN: 1596-5996 *

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