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WO2016017894A1 - Composition cosmétique contenant un extrait de riz sec et son procédé de préparation - Google Patents

Composition cosmétique contenant un extrait de riz sec et son procédé de préparation Download PDF

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Publication number
WO2016017894A1
WO2016017894A1 PCT/KR2015/003016 KR2015003016W WO2016017894A1 WO 2016017894 A1 WO2016017894 A1 WO 2016017894A1 KR 2015003016 W KR2015003016 W KR 2015003016W WO 2016017894 A1 WO2016017894 A1 WO 2016017894A1
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Prior art keywords
extract
cosmetic composition
rice
weight
parts
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English (en)
Korean (ko)
Inventor
홍소영
주광식
김혜진
한우리자랑
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AMI COSMETIC Co Ltd
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AMI COSMETIC Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9794—Liliopsida [monocotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin

Definitions

  • the present invention relates to a cosmetic composition comprising the extract of the field rice and a preparation method thereof.
  • melanin is synthesized by melanocytes (Melanocyte) present in the basal layer in the skin and transferred to surrounding keratinocytes, causing pigmentation of the skin.
  • Melanocyte melanocytes
  • skin whitening agents are developed to act on melanin production mechanisms to block melanocyte-associated signal transduction, to prevent melanin precursors from moving to keratinocytes, and to block melanin synthesis.
  • the research to be conducted is being actively conducted.
  • arbutin, kojic acid, hydroquinone and the like have been widely used as a whitening ingredient that inhibits the activity of tyrosinase, an enzyme required to synthesize melanin in the skin.
  • these substances have been reported to have side effects such as skin toxicity, skin cancer, skin irritation, etc. when used above a certain content, the use of the drug is strictly limited by the KFDA.
  • the most closely associated with wrinkles is the aging of skin cells.
  • the causes of skin aging can be classified into natural aging caused by aging and internal aging caused by the external environment. Natural aging is difficult to control because many genetic factors are involved. Therefore, much research is being conducted to control environmental factors such as ultraviolet rays, oxidation, and drying.
  • Environmental factors mainly cause collagen protein and elastin protein in the dermis, or degeneration, causing wrinkles.
  • the sun's ultraviolet rays are representative environmental factors that oxidize the skin and produce free radicals. This oxidative power and free radicals can cause protein breakdown, sugar oxidation, genetic modification, etc. in skin cells, which can cause aging and wrinkles on the skin.
  • Embodiments of the present invention can provide a cosmetic composition and a method for producing the same having excellent skin whitening, anti-wrinkle and skin anti-inflammatory effect.
  • Embodiments of the present invention can provide a cosmetic composition and a method for producing the same that can act on the skin to exhibit an antioxidant effect.
  • Embodiments of the present invention can provide a cosmetic composition and a method of manufacturing the same as there are less side effects such as skin irritation, cytotoxicity and the like.
  • One embodiment of the present invention relates to a cosmetic composition comprising a garden rice extract.
  • the field rice extract may be an outpost extract.
  • the field rice extract may be an extract of roots, leaves, stems, cultivars or mixtures thereof.
  • the garden rice may be Sangnam field rice varieties or agriculture and forestry or No. 1 varieties.
  • the content of the fertilized rice extract may be 0.01 to 50 parts by weight based on 100 parts by weight of the total cosmetic composition.
  • the fermented rice extract may contain p-coumaric acid, ferulic acid, isobitaxin and cyanidin.
  • the garden rice extract may further include a flavonoid compound.
  • the flavonoid compound may be at least one of ananthanthin, anthocyanin and catechin.
  • Another embodiment of the present invention relates to a method of preparing the cosmetic composition.
  • the production method comprises the steps of preparing a filtration extract from the paddy rice filtrate;
  • Preparing a primary concentrate by first concentrating the filtration extract; Preparing a fraction extract by fractionating the primary concentrate; And concentrating the fraction extract to produce a secondary concentrate; It may include.
  • Embodiments of the present invention can provide a cosmetic composition and a method for producing the same by inhibiting the activity of tyrosinase, inhibiting melanin production and showing skin whitening effect.
  • Embodiments of the present invention can provide a cosmetic composition and a method for producing the same that exhibits an anti-wrinkle effect through an elastase inhibitory action, an anti-inflammatory action and an antioxidant action.
  • Embodiments of the present invention can provide a cosmetic composition with low cytotoxicity, even if the content of the active ingredient is increased.
  • Figure 2 is a graph showing the results of the inhibitory factor inhibition experiment of the extract of Preparation Example 1.
  • Figure 3 is a graph showing the results of DPPH radical scavenging activity of the extract of Preparation Example 1.
  • Figure 4 is a graph showing the results of the elastase inhibition of the extract of Preparation Example 1.
  • Figure 5 is a graph showing the extracellular melanin production of the sample treated with the extract of Preparation Example 1.
  • One embodiment of the present invention relates to a cosmetic composition comprising a garden rice extract.
  • Cosmetic composition of one embodiment may include a garden rice extract.
  • Paddy rice unlike paddy rice, means rice having characteristics that are cultivated in the field.
  • paddy rice needs to be supplied with sufficient water for almost all growing periods, so it must be grown in paddy fields.
  • paddy rice is grown in the field because it consumes significantly less water compared to paddy rice during the growing season. This difference between paddy rice and paddy rice is thought to be due to the differentiation through long time evolution or breeding.
  • Rice and paddy rice may have, for example, a difference in phenotype or genotype in one or more properties. Due to this difference, the active ingredient contained in the rice paddies and the paddy rice may be different from each other.
  • Rice and paddy rice can be distinguished from one another through, for example, ecological characteristics, morphological characteristics or varieties.
  • paddy rice The ecological characteristics of paddy rice are, for example, that they are grown in a field, consume much less water compared to paddy during growth, have a drought-tolerant property, have a strong cold resistance, and are resistant to leaf blight. Point and strong lighting of chlorine chlorate (KClO3) are mentioned.
  • the morphological characteristics of the field rice are, for example, the point where the roots fall deeply into the ground (myocardial root), the roots have many roots, the thicker the leaves, the fewer the number of pores, and the higher the water storage capacity, the medium the stem and the water pipe, There are thick spots, large and dense spikes, and well-developed cilia.
  • the varieties of the field rice may be, for example, Sangnam field rice varieties or agriculture and forestry or No. 1 varieties.
  • the content of the paddy field active ingredient described later may be high, and extraction may be advantageous.
  • the field rice extract of one embodiment may be an outpost extract of the field rice.
  • the term "outpost" may mean a portion excluding the ear or rice grains of the plant to be extracted.
  • the paddy rice extract may be extracted from the outposts of paddy rice where the ears are removed by harvest. In this case, it is possible to prevent impurities due to rice grains or ear during the extraction process can be improved extraction efficiency.
  • the outpost of the harvested field rice not only can lower the manufacturing cost of the field rice extract, but also can smoothly supply the material can improve the efficiency of the manufacturing cost.
  • the field rice extract may be an extract of the root, leaves, stems, cultivars or mixtures thereof.
  • the field rice extract may be one extracted from a mixture of the roots and the Chongqing of the field rice.
  • the field rice extract may be one extracted from a mixture of stems and leaves of the field rice.
  • the paddy rice extract may be extracted from a mixture of roots, stems and leaves of the paddy rice. In this case, it is possible to adjust the content ratio of the active ingredients included in the paddy rice extract according to the desired purpose.
  • active ingredient may mean a component that can be contained in the composition to exhibit the desired effect.
  • the paddy rice extract of one embodiment may contain p-coumaric acid, ferulic acid, isobitaxin and cyanidin as active ingredients.
  • p-coumaric acid may act as a substance that inhibits the activity of tyrosinase. Inhibiting the activity of tyrosinase inhibits the production of melanin, and can impart a whitening effect to the cosmetic composition.
  • p-coumaric acid contained in the paddy rice extract at this time can lower the cytotoxicity compared to the synthetic material.
  • the p-coumaric acid may be, for example, trans-p-coumaric acid methyl ester.
  • the content of p-coumaric acid may be 0.001 parts by weight or more based on 100 parts by weight of the whole garden rice extract.
  • the upper limit of the p-coumaric acid content is not particularly limited but may be, for example, 5.0 parts by weight or less.
  • the content of p-coumaric acid may be from 0.001 part by weight to 1.0 part by weight based on 100 parts by weight of the whole garden rice extract.
  • the melanin synthesis inhibitory effect may be excellent while the extract manufacturing cost.
  • Ferulic acid acts as a material to neutralize free radicals, it can impart the effect of skin antioxidant to the cosmetic composition. Moreover, it can also play a role of suppressing tyrosinase, and can further improve the skin lightening effect.
  • the content of ferulic acid may be 0.001 parts by weight or more based on 100 parts by weight of the whole garden rice extract.
  • the upper limit of the ferulic acid content is not particularly limited but may be, for example, 5.0 parts by weight or less.
  • the content of p-coumaric acid may be from 0.001 part by weight to 1.0 part by weight based on 100 parts by weight of the whole garden rice extract. Within this range, the effect of antioxidant and skin whitening may be excellent, but the cost of extract preparation may be efficient.
  • Isovitexin acts as a substance that inhibits the production of free radicals, thereby imparting skin antioxidant effects to the cosmetic composition. In addition, it acts as an inhibitor of melanin production, can further improve the skin whitening effect, and also has the effect of inhibiting the activity of inflammatory factors.
  • the content of isobitaxin may be 0.001 parts by weight or more based on 100 parts by weight of the whole garden rice extract.
  • the upper limit of the content of isobitexin is not particularly limited but may be, for example, 5.0 parts by weight or less.
  • the content of isobitaxin may be 0.001 part by weight to 1.0 part by weight based on 100 parts by weight of the whole garden rice extract.
  • Cyanidin acts as a material to prevent skin inflammation caused by ultraviolet rays, it can impart an anti-inflammatory effect to the cosmetic composition.
  • the content of cyanidin may be 0.001 parts by weight or more based on 100 parts by weight of the whole garden rice extract.
  • the upper limit of the cyanidin content is not particularly limited but may be, for example, 5.0 parts by weight or less.
  • the content of cyanidin may be from 0.001 parts by weight to 1.0 parts by weight based on 100 parts by weight of the whole garden rice extract. Within this range, it is possible to provide a cosmetic composition exhibiting an anti-inflammatory effect while having an excellent effect of antioxidant and skin whitening.
  • the Paddy Rice Extract may contain additional flavonoid compounds, in addition to the aforementioned active ingredients. Flavonoid compounds can act as additional active ingredients, further improving the effects of antioxidant, skin inflammation inhibition and skin wrinkle prevention of the cosmetic composition.
  • the flavonoid compounds may, for example, contain one or more of anthanthin, anthocyanin and catechins alone or two or more in the form of a mixture.
  • the content of the flavonoid compound may be from 0.001 parts by weight to 1.0 parts by weight based on 100 parts by weight of the whole garden rice extract. Within this range, it can exhibit the effects of antioxidant, anti-inflammatory, etc. without inhibiting the activity of p-coumaric acid.
  • the extract of the field rice may include trans-para-coumaric acid methyl ester, ferulic acid, isobitaxin and cyanidin.
  • field extract may include trans-para-coumaric acid methyl ester, ferulic acid, isobitaxin, cyanidin and additional flavonoid compounds as active ingredients.
  • the extract of the field of these embodiments can implement the skin whitening effect through the inhibition of tyrosinase, the antioxidant effect through the elimination of free radicals, the anti-wrinkle effect through the inhibition of elastase.
  • the cosmetic composition contains a high concentration of the field extract of 100 ⁇ g / ml or more can inhibit the inflammation-inducing factor, it can reduce the skin toxicity.
  • the content of the field rice extract may be 0.01 to 50 parts by weight based on 100 parts by weight of the total cosmetic composition. For example, it may be 0.1 to 50 parts by weight, 1.0 to 50 parts by weight, 10 to 45 parts by weight, 10 to 35 parts by weight or 20 to 35 parts by weight. Within this range, the content of the active ingredient is high, while increasing the skin whitening, anti-wrinkle and anti-inflammatory effects, it is possible to maintain the stability of the formulation.
  • the cosmetic composition of another embodiment may include an excipient together with the above-mentioned extract.
  • the excipient may form a mixed solution, an emulsion and the like together with the extract of the garden rice to serve to impart proper dilution, firmness or viscosity to the cosmetic composition.
  • Excipients can be water soluble, oily or mixtures thereof.
  • the water-soluble raw material may play a role in controlling the concentration of the field rice extract and mixing well with the formulation. In addition, depending on the ingredients it can prevent the skin from drying out, it can serve to soothe.
  • the water-soluble raw material is not particularly limited as long as it can be used in cosmetics, for example, may be purified water, glycerin, propylene glycol, butylene glycol, polyethylene glycol, sorbitol, sodium hyaluronate.
  • Oily raw materials can be included in the cosmetic composition, to control the water loss of the skin and improve the absorption of the active ingredient.
  • the oily material is not particularly limited as long as it can be used in cosmetics, and may be, for example, oils, waxes, higher fatty acids, higher alcohols, hydrocarbons, esters, silicones, and the like.
  • Exemplary excipients may be used alone or in combination of two or more thereof.
  • Cosmetic compositions of yet another embodiment may further comprise an additive.
  • the additives may be surfactants, thickeners, pigments, preservatives, flavoring agents and the like.
  • the surfactant may serve to adsorb the extract and the excipient and mix well.
  • the surfactant is not particularly limited as long as it can be used in cosmetics, but may be, for example, an emulsifier, a solubilizer, a dispersant, a humectant, a foaming agent, an antifoaming agent, a cleaning agent, and the like.
  • the thickener may serve to maintain the viscosity of the cosmetic composition or to maintain the stability of the product.
  • the thickener is not particularly limited as long as it can be used in cosmetics, but may be, for example, guar gum, xanthan gum, gelatin, methylcellulose, alginate, polyvinyl alcohol, bentonite, and the like.
  • the pigment may serve to impart color to the cosmetic composition.
  • the pigment is not particularly limited as long as it can be used in cosmetics, and may be, for example, tar pigments, natural pigments, inorganic pigments, and the like.
  • the preservative may serve to prevent the cosmetic composition from being altered by the microorganism.
  • the preservative is not particularly limited as long as it can be used in cosmetics, but may be, for example, parabens, imidazolidinyl ureas, phenoxyethanol, phenonip, and the like.
  • Flavoring agent may serve to make a specific odor appear in the cosmetic composition, or to remove the odor of the raw material itself.
  • the flavoring agent is not particularly limited as long as it can be used in cosmetics, but may be, for example, aromatic chemicals, essential oils, natural extracts, aromas, and the like.
  • Exemplary additives may be used alone or in combination of two or more thereof.
  • the cosmetic composition may be prepared in any formulation commonly used in the art, and may be applied to cosmetics after formulation.
  • the cosmetic composition may be prepared, for example, in the form of a paste, cream, gel, powder, spray, solution, emulsion, suspension or the like.
  • Cosmetics containing such exemplary cosmetic compositions include, for example, skin lotions, skin softeners, skin toners, astringents, lotions, milk lotions, moisturizing lotions, nourishing lotions, massage creams, nourishing creams, moisturizing creams, hand creams, foundations, Essences, nutrition essences, packs, soaps, cleansing foam, cleansing lotion, cleansing cream, body lotion or body cleanser and the like, but is not limited thereto.
  • Formulation method of such a cosmetic composition and a method of manufacturing a cosmetic product may be applied by employing an appropriate one as necessary in the art well known in the art, but is not limited thereto.
  • Another embodiment of the present invention relates to a method for preparing a cosmetic composition comprising a garden rice extract.
  • the preparing of the filtration extract may include a method of preparing a field rice filtrate containing a garden rice and a solvent and a method of preparing a filter extract thereafter.
  • the field rice filtrate may refer to a solution containing an active ingredient derived from field rice.
  • the fermented rice filtrate may be prepared by adding 50 parts by weight to 200 parts by weight of an organic solvent to 100 parts by weight of rice, aging for 5 to 7 days, and then filtering. In the filtration process, the solids of the paddy rice may be removed.
  • the filtration method is not particularly limited and may be, for example, a reduced pressure filtration method. In this case, there is little loss of the active ingredient of the paddy rice extract, and the production time of the filtrate can be improved, which is advantageous for mass production and high concentration liquid preparation.
  • the method for preparing the filtrate extract may be an organic solvent extraction method or hot water extraction method.
  • the organic solvent extraction method may be a method of extracting by adding 10 parts by weight to 100 parts by weight of water and 100 parts by weight to 350 parts by weight of the organic solvent based on 100 parts by weight of the garden rice filtrate.
  • the organic solvent may be one or more of acetone, ethyl acetate, diethyl ether, benzene, chloroform, hexane or an alcohol having 1 to 10 carbon atoms.
  • the hot water extraction method may be, for example, a method in which 100 parts by weight to 500 parts by weight of water is added to the paddy rice filtrate with respect to 100 parts by weight of the paddy rice filtrate and is heated and extracted at 30 ° C. to 100 ° C. for 1 to 10 hours. have.
  • the step of preparing the primary concentrate may include a method of primary concentration of the filtration extract.
  • the primary concentration method may be, for example, a method of concentrating the aforementioned filtration extract by introducing it into a rotary vacuum evaporator (Eyela, Japan).
  • the step of preparing the fraction extract comprises a method for fractionating the active ingredient from the primary concentrate.
  • the primary concentrate may be placed in a separatory funnel, and a solvent for fractionation may be added.
  • the primary concentrate is put in a separatory funnel, and the active ingredient is extracted by adding a solvent for the first fraction.
  • the fraction extraction using the solvent for the first fraction is repeated 2 to 3 times, and then the active ingredient is extracted by adding the solvent for the second fraction.
  • the fractional solvent may be used without limitation depending on the polarity or solubility of the active ingredient of interest.
  • the solvent for fractionation may be hexane, ethyl acetate, butanol, or the like.
  • first to nth solvents for fractionation may be used.
  • N may be, for example, 1 to 4 or 1 to 3, but is not limited thereto.
  • the fraction using the solvent for fractionation may be 1 to 4 or 2 to 3 times for each solvent.
  • the weight ratio or volume ratio of the sample included in the fractionation solvent and the separating funnel may be 1: 1 to 1: 3, but is not limited thereto.
  • Each fraction obtained in this manner can be mixed to produce a fraction extract.
  • the preparing step of the secondary concentrate includes a method of secondary concentration of the fraction extract.
  • the secondary extract may be prepared by using the above-mentioned fraction extract using a rotary vacuum evaporator (Eyela, Japan).
  • Jeju paddy rice 2 kg was aged for 1 week in 4 kg of 70% ethanol and filtered under reduced pressure with filter paper to prepare a paddy rice filtrate.
  • the filter extract is prepared by concentrating a primary concentrate using a rotary vacuum evaporator (Eyela, Japan).
  • the primary concentrate is dissolved in a predetermined amount of water, and then put into a separatory funnel, and extracted by putting hexane into the separatory funnel at a 1: 1 ratio.
  • the fractions were repeated three times with ethyl acetate (1: 1 ratio), and the fractions were repeated three times with butanol (1: 1 ratio), and each fraction was mixed to prepare a fraction extract.
  • the fraction extract is concentrated with a rotary vacuum evaporator (Eyela, Japan) to obtain a secondary concentrate.
  • the secondary concentrate thus prepared was applied as in Example 1 below using the extract of the field rice.
  • Purified water, glycerin, and propylene glycol are heated to 70 ° C., and an oily higher fatty acid, emulsifier, and preservative are heated to 70 ° C. and dissolved to add emulsified solution. After the emulsification is completed, the solution is cooled to 45 ° C., and the paddy rice extract obtained in Preparation Example 1 and the flavor are added and dispersed. After cooling to 30 °C to prepare a cream containing a paddy rice extract.
  • binding energy for tyrosinase of the Paddy Rice Extract prepared in Preparation Example 1 was measured.
  • binding energy values of arbutin, ascorbic acid and niacinamide as tyrosinase were measured.
  • X-ray crystal structure of tyrosinase as a receptor was used two deoxy-form (2ZMX) and oxy-form (1WX2) detected from the RCSB protein data bank (PDB).
  • the deoxy-form is a form in which only two copper atoms (Cu) are present at the reaction point, and the oxy-form is a structure in which oxygen atoms exist in the form of peroxides between the two copper atoms.
  • docking score uses a scoring function, which is based on the binding affinity (Kd) of the X-ray crystal structure and protein-ligand complex compound.
  • the extract of the field of Preparation Example 1 was lower than that of arbutin, but showed higher binding energy values than ascorbic acid and niacinamide. Through this, it was found that the field rice extract had a higher tyrosinase inhibitory effect than the whitening substance reported by KFDA, niacinamide, ascorbic acid.
  • the paddy rice extract prepared in Preparation Example 1 was diluted to concentrations of 0, 3.125, 6.25, 12.5, 25, 50, and 100 ( ⁇ g / ml), respectively, to evaluate the degree of cytotoxicity according to the concentration.
  • RAW 264.7 cells a type of macrophages, were counted and distributed equally in 1.0 ⁇ 10 4 cells / well on a 24well plate using DMEM medium containing 1% penicillin / scrapomycin and 10% FBS (fetal bovineserum). Incubated for 24 hours at 37 °C, 5% carbon dioxide conditions.
  • WST-1 assay solution (ez-cytox) was added to each well, followed by reaction for 2 hours in an incubator, and the absorbance at 450 nm was measured by ELISA reader to confirm cell viability.
  • the extract of Preparation Example 1 showed little cytotoxicity up to a concentration of 100 ⁇ g / ml.
  • the field rice extract of Preparation Example 1 was found to have very little cytotoxicity even when used at high concentration.
  • Nitric Oxide The degree of inhibition of Nitric Oxide (NO) was measured by diluting the extracts prepared in Preparation Example 1 to concentrations of 0, 3.125, 6.25, 12.5, 25, 50 and 100 ( ⁇ g / ml), respectively.
  • RAW 264.7 cells a type of macrophages, were counted in the same manner by dividing 1.0 ⁇ 10 4 cells / well into 24well plates using DMEM medium containing 1% penicillin / scrap mycin and 10% FBS (fetal bovineserum). Incubated for 24 hours at 37 °C, 5% carbon dioxide conditions.
  • LPS lipo poly saccharide
  • the field extract of Preparation Example 1 can inhibit the activity of inflammation-induced factors according to the concentration, and hardly induces cytotoxicity even at a concentration of 100 ⁇ g / ml.
  • the PPH extract prepared in Preparation Example 1 was diluted to concentrations of 0, 1.5625, 3.125, 6.25, 12.5, 25, 50 and 100 ( ⁇ g / ml), respectively, to evaluate the degree of DPPH inhibition according to the concentration.
  • 0.1M DPPH 250 ⁇ l (1,1-diphenyl-2-picrylhydrazyl) solution was mixed and reacted at 4 ° C. for 30 minutes. After the reaction, each sample was placed in a 96well plate and the absorbance was measured at 520 nm using an ELISA reader.
  • the free radical scavenging activity (%) of the sample was calculated using Equation 1 below as a control when no sample was added. At this time, ascorbic acid was used as a positive control.
  • Free radical scavenging ability [100- (Treat the absorbance / sample of the sample treated group
  • the extract of the field rice of Preparation Example 1 showed DPPH scavenging ability in a concentration-dependent manner. Accordingly, it was found that the paddy rice extract of Preparation Example 1 has an antioxidant effect and an effect of preventing wrinkles caused by active oxygen.
  • N-succinyl- (Ala) 3-p-nitroanilide (S4760, Sigma) dissolved in 10mM Tris-HCl buffer (pH8.0) at different concentrations and 1.6 mM concentration of Elastase from porcine pancreas Type IV ( E0258, Sigma) 0.4U / mL was mixed and reacted at 25 ° C. for 5 minutes, and then absorbed at 410 nm in a 96well plate.
  • Elastase inhibitory activity (%) 100- (absorbance of sample treated group / absorbance of sample not treated ⁇ 100)
  • the field rice extract of Preparation Example 1 exhibited a high elastase inhibitory effect in a concentration-dependent manner, indicating an effect on wrinkle improvement.
  • the amount of melanin inhibition according to the concentration was measured by diluting the extracts prepared in Preparation Example 1 to concentrations of 0, 3.125, 6.25, 12.5, 25, 50, 100, 200 and 400 ( ⁇ g / ml), respectively.
  • B16F10 melanoma cells were counted equally by 2.0 ⁇ 10 5 cell / well per dish in 60 ⁇ Petri dish using a hemacytometer, and then incubated for 24 hours at 37 ° C. and 5% carbon dioxide conditions. .
  • the obtained cell culture solution was removed, the Jeju Paddy Rice extract was taken at each concentration with ⁇ -MSH50nM for inducing melanin production, mixed with the medium and allowed to react for 60 hours, and then cultured (supernatant). Collected.
  • the culture solution (supernatant) reacted for 60 hours was measured by absorbance at 450nm using an ELISA plate reader to reduce the extracellular melanin production. And the extracellular melanin production amount was calculated using the following equation (3).
  • the extract of the field rice of Preparation Example 1 has an effect of suppressing the production of melanin. Accordingly, it was found that the field rice extract of Preparation Example 1 has a skin whitening effect.
  • Cosmetic composition comprising the extract of the field rice according to the present invention and a method for manufacturing the same may inhibit the activity of tyrosinase to inhibit the melanin production and provide a cosmetic showing the skin whitening effect to satisfy the needs of consumers.

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Abstract

Une composition cosmétique selon un mode de réalisation de la présente invention peut contenir un extrait de riz sec. L'extrait de riz sec contient de l'acide p-coumarique, de l'acide felulique, de l'isovitexine, et de la cyanidine, et peut ainsi présenter des effets de blanchiment de la peau, des effets anti-rides et des effets anti-inflammatoires.
PCT/KR2015/003016 2014-07-30 2015-03-27 Composition cosmétique contenant un extrait de riz sec et son procédé de préparation Ceased WO2016017894A1 (fr)

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KR20140097627 2014-07-30
KR10-2014-0097627 2014-07-30

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019066606A1 (fr) * 2017-09-29 2019-04-04 대봉엘에스 주식회사 Compositions cosmétiques et pharmaceutiques contenant chacune un extrait d'aloès et un extrait de riz pluvial
KR20200082812A (ko) * 2018-12-31 2020-07-08 대봉엘에스 주식회사 알로에 추출물, 밭벼 추출물 및 세라마이드를 포함하는 화장료 및 약학적 조성물

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JPH08239661A (ja) * 1995-02-28 1996-09-17 Ibaraki Pref Gov 土壌改良剤及びその使用方法
KR20120058781A (ko) * 2010-11-30 2012-06-08 고려대학교 산학협력단 칠보벼 추출물을 유효성분으로 함유하는 피부 미백용 조성물
JP2013103906A (ja) * 2011-11-14 2013-05-30 Kyoei Kagaku Kogyo Kk 化粧料
KR20140006617A (ko) * 2012-07-06 2014-01-16 보령제약 주식회사 벼줄기세포 고온고압 추출물을 함유하는 미백용 화장료 조성물

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Publication number Priority date Publication date Assignee Title
WO2019066606A1 (fr) * 2017-09-29 2019-04-04 대봉엘에스 주식회사 Compositions cosmétiques et pharmaceutiques contenant chacune un extrait d'aloès et un extrait de riz pluvial
KR20190037631A (ko) * 2017-09-29 2019-04-08 대봉엘에스 주식회사 알로에 추출물 및 밭벼 추출물을 포함하는 화장료 및 약학적 조성물
KR101987719B1 (ko) * 2017-09-29 2019-06-11 대봉엘에스 주식회사 알로에 추출물 및 밭벼 추출물을 포함하는 화장료 및 약학적 조성물
US11607437B2 (en) 2017-09-29 2023-03-21 Daebong Ls, Ltd Cosmetic and pharmaceutical compositions each containing aloe extract and upland rice extract
KR20200082812A (ko) * 2018-12-31 2020-07-08 대봉엘에스 주식회사 알로에 추출물, 밭벼 추출물 및 세라마이드를 포함하는 화장료 및 약학적 조성물
KR102181893B1 (ko) * 2018-12-31 2020-11-23 대봉엘에스 주식회사 알로에 추출물, 밭벼 추출물 및 세라마이드를 포함하는 화장료 및 약학적 조성물

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