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WO2009140191A3 - Detection of disease related gene mutations by single molecule amplification with multiplex ligation assays - Google Patents

Detection of disease related gene mutations by single molecule amplification with multiplex ligation assays Download PDF

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Publication number
WO2009140191A3
WO2009140191A3 PCT/US2009/043436 US2009043436W WO2009140191A3 WO 2009140191 A3 WO2009140191 A3 WO 2009140191A3 US 2009043436 W US2009043436 W US 2009043436W WO 2009140191 A3 WO2009140191 A3 WO 2009140191A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection
single molecule
related gene
disease related
gene mutations
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2009/043436
Other languages
French (fr)
Other versions
WO2009140191A2 (en
Inventor
Jun Zhu
Qin Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BioMedomics Inc
Original Assignee
BioMedomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BioMedomics Inc filed Critical BioMedomics Inc
Publication of WO2009140191A2 publication Critical patent/WO2009140191A2/en
Publication of WO2009140191A3 publication Critical patent/WO2009140191A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Taught herein is a method of multiplexing mutation detection strategy of the PASS platform using oligonucleotide ligation assay. To increase the throughput of mutation detection procedure of the PASS platform, oligonucleotide ligation assay (OLA) replaces single-base extension (SBE), which allows for interrogation of multiple drug-resistance mutations in each base-detection cycle. Also taught herein is the development of a new generation of PASS platform using bridge PCR amplification. The entire procedure may be automated so that a new generation of PASS platform is suitable for routine clinical practice.
PCT/US2009/043436 2008-05-12 2009-05-11 Detection of disease related gene mutations by single molecule amplification with multiplex ligation assays Ceased WO2009140191A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12728508P 2008-05-12 2008-05-12
US61/127,285 2008-05-12

Publications (2)

Publication Number Publication Date
WO2009140191A2 WO2009140191A2 (en) 2009-11-19
WO2009140191A3 true WO2009140191A3 (en) 2010-03-04

Family

ID=41319270

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/043436 Ceased WO2009140191A2 (en) 2008-05-12 2009-05-11 Detection of disease related gene mutations by single molecule amplification with multiplex ligation assays

Country Status (1)

Country Link
WO (1) WO2009140191A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107385030A (en) * 2017-07-14 2017-11-24 广州精科医学检验所有限公司 Molecular label, the method for joint and determination containing low frequency mutant nucleic acid sequence
CN110468211B (en) * 2019-10-14 2020-02-14 湖南大地同年生物科技有限公司 Bladder cancer tumor mutant gene specific primer, kit and library construction method

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BENTLY D.R.: "Whole-genome re-sequencing", CURR. OPIN. GENET. DEV., vol. 16, no. 6, 18 October 2006 (2006-10-18), pages 545 - 552 *
CAI ET AL.: "Detection of minor drug-resistant populations by parallel allele-specific sequencing", NAT. METHODS, vol. 4, no. 2, 7 January 2007 (2007-01-07), pages L23 - 125 *
FEDURCO ET AL.: "BTA, a novel reagent for DNA attachment on glass and efficient generation of solid-phase amplified DNA colonies", NUCLEIC ACIDS RES., vol. 34, no. 3, E22, 9 February 2006 (2006-02-09), pages L-13 *
LIN ET AL.: "Recent patent and advances in the next-generation sequencing technologies", RECENT PAT. BIOMED. ENGINEER., vol. 1, no. 1, January 2008 (2008-01-01), pages 60 - 67 *

Also Published As

Publication number Publication date
WO2009140191A2 (en) 2009-11-19

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