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WO2008001376A3 - Detection of analytes in a medium - Google Patents

Detection of analytes in a medium Download PDF

Info

Publication number
WO2008001376A3
WO2008001376A3 PCT/IL2007/000794 IL2007000794W WO2008001376A3 WO 2008001376 A3 WO2008001376 A3 WO 2008001376A3 IL 2007000794 W IL2007000794 W IL 2007000794W WO 2008001376 A3 WO2008001376 A3 WO 2008001376A3
Authority
WO
WIPO (PCT)
Prior art keywords
sequence
analytes
detection
medium
construct
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IL2007/000794
Other languages
French (fr)
Other versions
WO2008001376A2 (en
Inventor
Itamar Willner
Yossi Weizmann
Moritz K Beissenhirtz
Zoya Cheglakov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yissum Research Development Co of Hebrew University of Jerusalem
Original Assignee
Yissum Research Development Co of Hebrew University of Jerusalem
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yissum Research Development Co of Hebrew University of Jerusalem filed Critical Yissum Research Development Co of Hebrew University of Jerusalem
Publication of WO2008001376A2 publication Critical patent/WO2008001376A2/en
Publication of WO2008001376A3 publication Critical patent/WO2008001376A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A nucleotide construct is provided that comprises a sequence A and a sequence B on a single nucleotide stretch, sequence B being on the 5' side of sequence A. Sequence A is complementary to sequence A'. Hybridization of sequence A' to the sequence A in the presence of a polymerase and nucleotides, yields synthesis of a complementary stretch of nucleic acids which comprises a sequence B' that is complementary to sequence B. Sequence B' being a reporting sequence that upon becoming freed from said construct after may be detected. This construct may be used for detection of analytes in a medium. The analyte may be a nucleotide sequence comprising sequence A', or a substrate that facilitates hybridization of sequence A' to A.
PCT/IL2007/000794 2006-06-28 2007-06-28 Detection of analytes in a medium Ceased WO2008001376A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81687106P 2006-06-28 2006-06-28
US60/816,871 2006-06-28

Publications (2)

Publication Number Publication Date
WO2008001376A2 WO2008001376A2 (en) 2008-01-03
WO2008001376A3 true WO2008001376A3 (en) 2008-04-10

Family

ID=38846101

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2007/000794 Ceased WO2008001376A2 (en) 2006-06-28 2007-06-28 Detection of analytes in a medium

Country Status (1)

Country Link
WO (1) WO2008001376A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2287334A1 (en) * 2009-08-21 2011-02-23 Qiagen GmbH Method for documenting nucleic acids
US9845495B2 (en) 2010-12-10 2017-12-19 Abbott Laboratories Method and kit for detecting target nucleic acid
US9335292B2 (en) 2011-10-13 2016-05-10 Auburn University Electrochemical proximity assay
CN103060282A (en) * 2013-02-25 2013-04-24 威海东宝制药有限公司 DNA peroxidase and preparation method and application thereof
WO2015114469A2 (en) 2014-01-15 2015-08-06 Abbott Japan Co., Ltd Covered sequence conversion dna and detection methods
JP6773651B2 (en) 2014-10-14 2020-10-21 アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ Sequence conversion and signal amplification DNA having a poly DNA spacer sequence and detection methods using them
US10604790B2 (en) 2014-12-24 2020-03-31 Abbott Laboratories Sequence conversion and signal amplifier DNA cascade reactions and detection methods using same
WO2016196691A2 (en) 2015-06-01 2016-12-08 California Institute Of Technology Compositions and methods for screening t cells with antigens for specific populations
WO2018165475A1 (en) 2017-03-08 2018-09-13 California Institute Of Technology Pairing antigen specificity of a t cell with t cell receptor sequences
WO2018165566A1 (en) 2017-03-09 2018-09-13 Auburn University Differential circuit for background correction in electrochemical measurements
WO2019010341A1 (en) 2017-07-07 2019-01-10 Innamed, Inc. Aptamers for measuring lipoprotein levels
CN107988321A (en) * 2017-10-27 2018-05-04 中国农业大学 A kind of nucleic acid sensor of resistance to high salt of mercury and its application
US11560565B2 (en) 2018-06-13 2023-01-24 Auburn University Electrochemical detection nanostructure, systems, and uses thereof
CN112557659B (en) * 2019-09-25 2021-11-23 南京大学 Preparation and application of multiple signal amplification biosensor for detecting MUC1

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6140055A (en) * 1998-03-05 2000-10-31 Johnson & Johnson Research Pty Limited Zymogenic nucleic acid detection methods and related kits
US20040009510A1 (en) * 2000-03-06 2004-01-15 Scott Seiwert Allosteric nucleic acid sensor molecules
WO2004059005A2 (en) * 2002-10-29 2004-07-15 Guoliang Fu Combined exponential and linear amplification
US20050250134A1 (en) * 2004-03-02 2005-11-10 Dawei Sheng Fluorescent energy transfer labeled nucleic acid substrates and methods of use thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6140055A (en) * 1998-03-05 2000-10-31 Johnson & Johnson Research Pty Limited Zymogenic nucleic acid detection methods and related kits
US20040009510A1 (en) * 2000-03-06 2004-01-15 Scott Seiwert Allosteric nucleic acid sensor molecules
WO2004059005A2 (en) * 2002-10-29 2004-07-15 Guoliang Fu Combined exponential and linear amplification
US20050250134A1 (en) * 2004-03-02 2005-11-10 Dawei Sheng Fluorescent energy transfer labeled nucleic acid substrates and methods of use thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SANDO S ET AL: "Amplified nucleic acid sensing using programmed self-cleaving DNAzyme", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 125, no. 51, 3 December 2003 (2003-12-03), pages 15720 - 15721, XP002986218, ISSN: 0002-7863 *

Also Published As

Publication number Publication date
WO2008001376A2 (en) 2008-01-03

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