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WO2008129699A1 - Fusicoccane synthase chimérique et gène de celle-ci - Google Patents

Fusicoccane synthase chimérique et gène de celle-ci Download PDF

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Publication number
WO2008129699A1
WO2008129699A1 PCT/JP2007/069337 JP2007069337W WO2008129699A1 WO 2008129699 A1 WO2008129699 A1 WO 2008129699A1 JP 2007069337 W JP2007069337 W JP 2007069337W WO 2008129699 A1 WO2008129699 A1 WO 2008129699A1
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme
gene
fusicocca
diene
producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2007/069337
Other languages
English (en)
Japanese (ja)
Inventor
Tomonobu Toyomasu
Takeshi Sassa
Tohru Dairi
Nobuo Kato
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Osaka NUC
Original Assignee
Osaka University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Osaka University NUC filed Critical Osaka University NUC
Publication of WO2008129699A1 publication Critical patent/WO2008129699A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P15/00—Preparation of compounds containing at least three condensed carbocyclic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention vise à proposer une nouvelle enzyme et son gène qui peuvent être utilisés de façon efficace dans la production à grande échelle de fusicocca-2,10(14)-diène qui est utile comme matière pour produire la cotylénine, les 12-désoxyfusicoccines et leurs dérivés ayant un grand potentiel comme agent anti-cancer. On vise également à proposer un procédé de fabrication de fusicocca-2,10(14)-diène à l'aide de l'enzyme décrite ci-dessus ou d'un vecteur à utiliser dans la production de l'enzyme et d'un transformant. Une fusicoccane synthase chimérique qui est caractérisée comme ayant un domaine de terpène cyclase comprenant la séquence d'acides aminés représentée SEQ ID NO:1 ou une séquence fonctionnelle équivalente à celle-ci dans la région N-terminale et un domaine de prényl transférase comprenant la séquence d'acides aminés représentée par SEQ ID NO:2 ou une séquence équivalent fonctionnel à celle-ci dans la région C-terminale. Par la culture d'un transformant qui porte un gène codant pour l'enzyme décrite ci-dessus, l'enzyme et le fusicocca-2,10(14)-diène sont obtenus.
PCT/JP2007/069337 2007-03-30 2007-10-03 Fusicoccane synthase chimérique et gène de celle-ci Ceased WO2008129699A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007094968A JP2008245628A (ja) 2007-03-30 2007-03-30 フシコッカン合成キメラ型酵素およびその遺伝子
JP2007-094968 2007-03-30

Publications (1)

Publication Number Publication Date
WO2008129699A1 true WO2008129699A1 (fr) 2008-10-30

Family

ID=39875240

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2007/069337 Ceased WO2008129699A1 (fr) 2007-03-30 2007-10-03 Fusicoccane synthase chimérique et gène de celle-ci

Country Status (2)

Country Link
JP (1) JP2008245628A (fr)
WO (1) WO2008129699A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010104763A1 (fr) * 2009-03-11 2010-09-16 Sapphire Energy, Inc. Production de biocarburant dans des procaryotes et eucaryotes
WO2024050554A3 (fr) * 2022-09-02 2024-05-02 University Of Florida Research Foundation, Incorporated Synthèse de cotylénine et d'analogues de celle-ci pour le développement de modulateurs sélectifs de l'interaction protéine-protéine 14-3-3

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
SASSA T. ET AL.: "Fusicocca-3(16), 10(14)-diene, and beta- and delta-araneosense, new fusicoccin biosynthesis-related diterpene hydrocarbons from Phomopsis amygdale", BIOSCI. BIOTECHNOL. BIOCHEM., vol. 68, no. 7, 2004, pages 1608 - 1610, XP003023921 *
TOYOMASU T. ET AL.: "Fusicoccins are biosynthesized by an unusual chimera diterpene synthase in fungi", PROC. NATL. ACAD. SCI. U.S.A., vol. 104, no. 9, February 2007 (2007-02-01), pages 3084 - 3088, XP003023920 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010104763A1 (fr) * 2009-03-11 2010-09-16 Sapphire Energy, Inc. Production de biocarburant dans des procaryotes et eucaryotes
US20120058535A1 (en) * 2009-03-11 2012-03-08 Sapphire Energy, Inc. Biofuel production in prokaryotes and eukaryotes
WO2024050554A3 (fr) * 2022-09-02 2024-05-02 University Of Florida Research Foundation, Incorporated Synthèse de cotylénine et d'analogues de celle-ci pour le développement de modulateurs sélectifs de l'interaction protéine-protéine 14-3-3

Also Published As

Publication number Publication date
JP2008245628A (ja) 2008-10-16

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