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WO2008129699A1 - Chimeric fusicoccane synthase and gene of the same - Google Patents

Chimeric fusicoccane synthase and gene of the same Download PDF

Info

Publication number
WO2008129699A1
WO2008129699A1 PCT/JP2007/069337 JP2007069337W WO2008129699A1 WO 2008129699 A1 WO2008129699 A1 WO 2008129699A1 JP 2007069337 W JP2007069337 W JP 2007069337W WO 2008129699 A1 WO2008129699 A1 WO 2008129699A1
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme
gene
fusicocca
diene
producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2007/069337
Other languages
French (fr)
Japanese (ja)
Inventor
Tomonobu Toyomasu
Takeshi Sassa
Tohru Dairi
Nobuo Kato
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Osaka NUC
Original Assignee
Osaka University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Osaka University NUC filed Critical Osaka University NUC
Publication of WO2008129699A1 publication Critical patent/WO2008129699A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P15/00—Preparation of compounds containing at least three condensed carbocyclic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

It is intended to provide a novel enzyme and its gene which can be effectively used in the large-scale production of fusicocca-2,10(14)-diene that is useful as a material for producing cotylenin, 12-deoxyfusicoccins and derivatives thereof having great potential as an anticancer agent. It is also intended to provide a method of producing fusicocca-2,10(14)-diene by using the above-described enzyme or a vector to be used in producing the enzyme and a transformant. A chimeric fusicoccane synthase which is characterized by having a terpene cyclase domain comprising the amino acid sequence represented by SEQ ID NO:1 or a sequence functional equivalent thereto in the N-terminal region and a prenyl transferase domain comprising the amino acid sequence represented by SEQ ID NO:2 or a sequence functional equivalent thereto in the C-terminal region. By culturing a transformant which carries a gene encoding the above-described enzyme, the enzyme and fusicocca-2,10(14)-diene are produced.
PCT/JP2007/069337 2007-03-30 2007-10-03 Chimeric fusicoccane synthase and gene of the same Ceased WO2008129699A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007094968A JP2008245628A (en) 2007-03-30 2007-03-30 Fusicoccan synthetic chimeric enzyme and its gene
JP2007-094968 2007-03-30

Publications (1)

Publication Number Publication Date
WO2008129699A1 true WO2008129699A1 (en) 2008-10-30

Family

ID=39875240

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2007/069337 Ceased WO2008129699A1 (en) 2007-03-30 2007-10-03 Chimeric fusicoccane synthase and gene of the same

Country Status (2)

Country Link
JP (1) JP2008245628A (en)
WO (1) WO2008129699A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010104763A1 (en) * 2009-03-11 2010-09-16 Sapphire Energy, Inc. Biofuel production in prokaryotes and eukaryotes
WO2024050554A3 (en) * 2022-09-02 2024-05-02 University Of Florida Research Foundation, Incorporated Synthesis of cotylenin and analogs thereof for the development of selective modulators of 14-3-3 protein-protein interaction

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
SASSA T. ET AL.: "Fusicocca-3(16), 10(14)-diene, and beta- and delta-araneosense, new fusicoccin biosynthesis-related diterpene hydrocarbons from Phomopsis amygdale", BIOSCI. BIOTECHNOL. BIOCHEM., vol. 68, no. 7, 2004, pages 1608 - 1610, XP003023921 *
TOYOMASU T. ET AL.: "Fusicoccins are biosynthesized by an unusual chimera diterpene synthase in fungi", PROC. NATL. ACAD. SCI. U.S.A., vol. 104, no. 9, February 2007 (2007-02-01), pages 3084 - 3088, XP003023920 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010104763A1 (en) * 2009-03-11 2010-09-16 Sapphire Energy, Inc. Biofuel production in prokaryotes and eukaryotes
US20120058535A1 (en) * 2009-03-11 2012-03-08 Sapphire Energy, Inc. Biofuel production in prokaryotes and eukaryotes
WO2024050554A3 (en) * 2022-09-02 2024-05-02 University Of Florida Research Foundation, Incorporated Synthesis of cotylenin and analogs thereof for the development of selective modulators of 14-3-3 protein-protein interaction

Also Published As

Publication number Publication date
JP2008245628A (en) 2008-10-16

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