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WO2008146019A3 - Method for baculovirus generation from insect cells in high-troughput plates - Google Patents

Method for baculovirus generation from insect cells in high-troughput plates Download PDF

Info

Publication number
WO2008146019A3
WO2008146019A3 PCT/GB2008/001867 GB2008001867W WO2008146019A3 WO 2008146019 A3 WO2008146019 A3 WO 2008146019A3 GB 2008001867 W GB2008001867 W GB 2008001867W WO 2008146019 A3 WO2008146019 A3 WO 2008146019A3
Authority
WO
WIPO (PCT)
Prior art keywords
insect cells
troughput
plates
baculovirus generation
baculovirus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2008/001867
Other languages
French (fr)
Other versions
WO2008146019A2 (en
WO2008146019A8 (en
Inventor
Kari Juhani Airenne
Seppo Ylae-Herttuala
Hanna-Riikka Kaerkkaeinen
Olli Laitinen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ark Therapeutics Ltd
Original Assignee
Ark Therapeutics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ark Therapeutics Ltd filed Critical Ark Therapeutics Ltd
Publication of WO2008146019A2 publication Critical patent/WO2008146019A2/en
Publication of WO2008146019A3 publication Critical patent/WO2008146019A3/en
Publication of WO2008146019A8 publication Critical patent/WO2008146019A8/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14121—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/103—Plasmid DNA for invertebrates
    • C12N2800/105—Plasmid DNA for invertebrates for insects

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention is a method of baculovirus generation from insect cells, using a high-throughput plate, wherein the insect cells are transfected or infected by a virus construct, in suspension, and wherein the plate comprises wells which have a substantially square cross-section.
PCT/GB2008/001867 2007-06-01 2008-06-02 Method for baculovirus generation from insect cells in high-troughput plates Ceased WO2008146019A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0710535A GB0710535D0 (en) 2007-06-01 2007-06-01 Baculovirus generation
GB0710535.6 2007-06-01

Publications (3)

Publication Number Publication Date
WO2008146019A2 WO2008146019A2 (en) 2008-12-04
WO2008146019A3 true WO2008146019A3 (en) 2009-01-15
WO2008146019A8 WO2008146019A8 (en) 2009-07-16

Family

ID=38289720

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2008/001867 Ceased WO2008146019A2 (en) 2007-06-01 2008-06-02 Method for baculovirus generation from insect cells in high-troughput plates

Country Status (2)

Country Link
GB (1) GB0710535D0 (en)
WO (1) WO2008146019A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115791575A (en) * 2022-08-26 2023-03-14 武汉华美生物工程有限公司 Method for determining titer of recombinant baculovirus based on flow cytometry

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999045130A1 (en) * 1998-03-04 1999-09-10 Onyx Pharmaceuticals, Inc. Baculovirus expression system and method for high throughput expression of genetic material
WO2003076606A1 (en) * 2002-03-09 2003-09-18 Neurogenex Co., Ltd. High throughput system for producing recombinant viruses using site-specific recombination

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999045130A1 (en) * 1998-03-04 1999-09-10 Onyx Pharmaceuticals, Inc. Baculovirus expression system and method for high throughput expression of genetic material
WO2003076606A1 (en) * 2002-03-09 2003-09-18 Neurogenex Co., Ltd. High throughput system for producing recombinant viruses using site-specific recombination

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ALBALA JOANNA S ET AL: "From genes to proteins: High-throughput expression and purification of the human proteome", JOURNAL OF CELLULAR BIOCHEMISTRY, vol. 80, no. 2, 20 October 2000 (2000-10-20), pages 187 - 191, XP002501816, ISSN: 0730-2312 *
LAITINEN OLLI H ET AL: "A multipurpose vector system for the screening of libraries in bacteria, insect and mammalian cells and expression in vivo.", NUCLEIC ACIDS RESEARCH 2005, vol. 33, no. 4, 2005, pages e42, XP002501818, ISSN: 1362-4962 *
MCCALL E J ET AL: "Improvements to the throughput of recombinant protein expression in the baculovirus/insect cell system", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, vol. 42, no. 1, 1 July 2005 (2005-07-01), pages 29 - 36, XP004918444, ISSN: 1046-5928 *
REDAELLI ET AL: "A platform for high-throughput expression of recombinant human enzymes secreted by insect cells", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 120, no. 1, 17 October 2005 (2005-10-17), pages 59 - 71, XP005097886, ISSN: 0168-1656 *
SHRESTHA BINESH ET AL: "Baculovirus expression vector system: an emerging host for high-throughput eukaryotic protein expression.", METHODS IN MOLECULAR BIOLOGY (CLIFTON, N.J.) 2008, vol. 439, 28 March 2008 (2008-03-28), pages 269 - 289, XP002501817, ISSN: 1064-3745 *

Also Published As

Publication number Publication date
WO2008146019A2 (en) 2008-12-04
GB0710535D0 (en) 2007-07-11
WO2008146019A8 (en) 2009-07-16

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