[go: up one dir, main page]

WO2008146019A8 - Method for baculovirus generation from insect cells in high-troughput plates - Google Patents

Method for baculovirus generation from insect cells in high-troughput plates Download PDF

Info

Publication number
WO2008146019A8
WO2008146019A8 PCT/GB2008/001867 GB2008001867W WO2008146019A8 WO 2008146019 A8 WO2008146019 A8 WO 2008146019A8 GB 2008001867 W GB2008001867 W GB 2008001867W WO 2008146019 A8 WO2008146019 A8 WO 2008146019A8
Authority
WO
WIPO (PCT)
Prior art keywords
insect cells
troughput
plates
baculovirus generation
baculovirus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2008/001867
Other languages
French (fr)
Other versions
WO2008146019A3 (en
WO2008146019A2 (en
Inventor
Kari Juhani Airenne
Seppo Ylae-Herttuala
Hanna-Riikka Kaerkkaeinen
Olli Laitinen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ark Therapeutics Ltd
Original Assignee
Ark Therapeutics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ark Therapeutics Ltd filed Critical Ark Therapeutics Ltd
Publication of WO2008146019A2 publication Critical patent/WO2008146019A2/en
Publication of WO2008146019A3 publication Critical patent/WO2008146019A3/en
Publication of WO2008146019A8 publication Critical patent/WO2008146019A8/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14121—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/103—Plasmid DNA for invertebrates
    • C12N2800/105—Plasmid DNA for invertebrates for insects

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention is a method of baculovirus generation from insect cells, using a high-throughput plate, wherein the insect cells are transfected or infected by a virus construct, in suspension, and wherein the plate comprises wells which have a substantially square cross-section.
PCT/GB2008/001867 2007-06-01 2008-06-02 Method for baculovirus generation from insect cells in high-troughput plates Ceased WO2008146019A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0710535A GB0710535D0 (en) 2007-06-01 2007-06-01 Baculovirus generation
GB0710535.6 2007-06-01

Publications (3)

Publication Number Publication Date
WO2008146019A2 WO2008146019A2 (en) 2008-12-04
WO2008146019A3 WO2008146019A3 (en) 2009-01-15
WO2008146019A8 true WO2008146019A8 (en) 2009-07-16

Family

ID=38289720

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2008/001867 Ceased WO2008146019A2 (en) 2007-06-01 2008-06-02 Method for baculovirus generation from insect cells in high-troughput plates

Country Status (2)

Country Link
GB (1) GB0710535D0 (en)
WO (1) WO2008146019A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115791575A (en) * 2022-08-26 2023-03-14 武汉华美生物工程有限公司 Method for determining titer of recombinant baculovirus based on flow cytometry

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2321261A1 (en) * 1998-03-04 1999-09-10 Onyx Pharmaceuticals, Inc. Baculovirus expression system and method for high throughput expression of genetic material
US7319001B2 (en) * 2002-03-09 2008-01-15 Neurogenex Co., Ltd. High throughput system for producing recombinant viruses using site-specific recombination

Also Published As

Publication number Publication date
WO2008146019A3 (en) 2009-01-15
WO2008146019A2 (en) 2008-12-04
GB0710535D0 (en) 2007-07-11

Similar Documents

Publication Publication Date Title
WO2007084238A3 (en) Adjustable bone plate
BRPI1008634A2 (en) "a means of connection, a method of fabrication thereof and a material connection".
BRPI0814459A2 (en) INSECT CELL, METHOD FOR PRODUCING A RECOMBINANT PARVOVIRAL VIRION IN AN INSERT CELL, AND NUCLEIC ACID CONSTRUCTION.
IL190906A0 (en) Influenza viruses able to infect canids, uses thereof
EP2058119A3 (en) Ceramic sheet and producing method thereof
EP2297303A4 (en) ENGINEERING OF THE CELL MEMBRANE
DK3081649T3 (en) MICROORGANISM PRODUCING L-METHIONIN PRECURSOR AND THE PROCEDURE FOR PRODUCING L-METHIONIN PRECURSOR BY USING THE MICROORGANISM
WO2006122295A8 (en) Methods of monitoring functional status of transplants using gene panels
FR2930558B1 (en) SILICA PARTICLE COMPRISING AN ORGANOLANTHANIDE COMPOUND, PROCESS FOR PREPARATION AND USES
WO2009077917A3 (en) Mems microphone
WO2006135904A3 (en) Method for producing improved results for applications which directly or indirectly utilize gene expression assay results
EP1869261A4 (en) Closet flange spacer
WO2009073448A3 (en) Construction system
WO2009111778A3 (en) Lymph nodes as a site for regeneration
EP1748790A4 (en) Multi plasmid system for the production of influenza virus
WO2008146019A3 (en) Method for baculovirus generation from insect cells in high-troughput plates
WO2009156770A9 (en) Elastic construction foundation method.
EP2023711B8 (en) Insect screening sheet
EP2168197B8 (en) Membrane electrode assembly
DK1562400T3 (en) Interchangeable hearing protection membrane for hearing aids
WO2008051266A3 (en) A dengue reporter virus and methods of making and using the same
AU2006212087A1 (en) Call notification controlled by call originating system
WO2007149896A3 (en) Dna fragmentation assay
Albu et al. An abstract Cogalois Theory for profinite groups
AU2007906399A0 (en) Hammer Lock Plates

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08762222

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase in:

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 08762222

Country of ref document: EP

Kind code of ref document: A2