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WO2008031430A3 - Doppelt transgenes nicht menschliches säugetier - Google Patents

Doppelt transgenes nicht menschliches säugetier Download PDF

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Publication number
WO2008031430A3
WO2008031430A3 PCT/DE2007/001703 DE2007001703W WO2008031430A3 WO 2008031430 A3 WO2008031430 A3 WO 2008031430A3 DE 2007001703 W DE2007001703 W DE 2007001703W WO 2008031430 A3 WO2008031430 A3 WO 2008031430A3
Authority
WO
WIPO (PCT)
Prior art keywords
tumours
human
cell
transgenic non
human mammal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE2007/001703
Other languages
English (en)
French (fr)
Other versions
WO2008031430A2 (de
Inventor
Juergen Borlak
Roman Halter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fraunhofer Gesellschaft zur Foerderung der Angewandten Forschung eV
Original Assignee
Fraunhofer Gesellschaft zur Foerderung der Angewandten Forschung eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fraunhofer Gesellschaft zur Foerderung der Angewandten Forschung eV filed Critical Fraunhofer Gesellschaft zur Foerderung der Angewandten Forschung eV
Publication of WO2008031430A2 publication Critical patent/WO2008031430A2/de
Publication of WO2008031430A3 publication Critical patent/WO2008031430A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/82—Translation products from oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0331—Animal model for proliferative diseases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/02—Cells from transgenic animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Veterinary Medicine (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Environmental Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Behavior & Ethology (AREA)
  • Physics & Mathematics (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Oncology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft transgene nicht-menschliche Säugetiere, die zwei Transgene in ihrem Genom tragen, ein Verfahren zu deren Herstellung, deren Verwendung und Zellgewebe, Zellen sowie Zellbestandteile daraus. Anwendungsgebiete der Erfindung sind die pharmazeutische Industrie und die Lebenswissenschaften: die Biologie, die Biochemie, die Biotechnologie, die Medizin, bevorzugt die Prophylaxe und die Therapie von Krebs, insbesondere Lungenkrebs. Der Erfindung liegt das Ziel zugrunde, ein verbessertes Tiermodell zu schaffen, das unterschiedliche Arten von Tumoren bildet, die mit menschlichen Tumoren, insbesondere Lungentumoren, und deren Metastasen vergleichbar sind. Aufgabe der Erfindung ist es daher, ein nicht menschliches Säugetier mit solchen verbesserten Eigenschaften und/oder dessen Zellgewebe, Zellen und Zellbestandteile, ein Verfahren zu deren Herstellung und deren Verwendung, sowie Mittel zu ihrer Herstellung und deren Verwendung, bereitzustellen. Diese Aufgabe wird mit einem doppelt transgenen nicht menschlichen Säugetier gelöst, dessen Genom a) eine kodierende DNA-Sequenz (1 ), die für ein Protoonkogen kodiert und die operativ mit wenigstens einer regulatorischen Sequenz verbunden ist, und b) eine kodierende DNA-Sequenz (2), die für ein Onkogen kodiert und die operativ mit wenigstens einer regulatorischen Sequenz verbunden ist, umfasst. Die Erfindung betrifft ferner entsprechend doppelt transgene Tumorzellklone für die Verwendung zur in vitro Untersuchung der Pathogenese von Lungentumoren und von gegen Lungenkarzinome gerichteten Substanzen bzw. Therapieverfahren.
PCT/DE2007/001703 2006-09-15 2007-09-17 Doppelt transgenes nicht menschliches säugetier Ceased WO2008031430A2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE102006044013.7 2006-09-15
DE102006044013 2006-09-15
DE102007003670A DE102007003670B4 (de) 2006-09-15 2007-01-18 Doppelt transgenes nicht menschliches Säugetier
DE102007003670.3 2007-01-18

Publications (2)

Publication Number Publication Date
WO2008031430A2 WO2008031430A2 (de) 2008-03-20
WO2008031430A3 true WO2008031430A3 (de) 2008-06-26

Family

ID=38896903

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2007/001703 Ceased WO2008031430A2 (de) 2006-09-15 2007-09-17 Doppelt transgenes nicht menschliches säugetier

Country Status (2)

Country Link
DE (1) DE102007003670B4 (de)
WO (1) WO2008031430A2 (de)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999028453A2 (de) * 1997-11-28 1999-06-10 Rapp Ulf R Transgenes nicht-menschliches säugetier mit einer onkogenen mutante des raf-1-gens

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999028453A2 (de) * 1997-11-28 1999-06-10 Rapp Ulf R Transgenes nicht-menschliches säugetier mit einer onkogenen mutante des raf-1-gens

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ALEXANDER W S ET AL: "ONCOGENE COOPERATION IN LYMPHOCYTE TRANSFORMATION MALIGNANT CONVERSION OF E-MU-MYC TRANSGENIC PRE-B CELLS IN-VITRO IS ENHANCED BY V-H-RAS OR V-RAF BUT NOT V-ABL", MOLECULAR AND CELLULAR BIOLOGY, vol. 9, no. 1, 1989, pages 67 - 73, XP002464890, ISSN: 0270-7306 *
BLASI E ET AL: "SELECTIVE IMMORTALIZATION OF MURINE MACROPHAGES FROM FRESH BONE MARROW BY A RAF/MYC RECOMBINANT MURINE RETROVIRUS", NATURE, NATURE PUBLISHING GROUP, LONDON, GB, vol. 318, no. 6047, 19 December 1985 (1985-12-19), pages 667 - 670, XP001146446, ISSN: 0028-0836 *
EHRHARDT A ET AL: "Development of pulmonary bronchiolo-alveolar adenocarcinomas in transgenic mice overexpressing murine c-myc and epidermal growth factor in alveolar type II pneumocytes", BRITISH JOURNAL OF CANCER, vol. 84, no. 6, 23 March 2001 (2001-03-23), pages 813 - 818, XP002464888, ISSN: 0007-0920 *
EHRHARDT A ET AL: "Increased susceptibility to the tobacco carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in transgenic mice overexpressing c-myc and epidermal growth factor in alveolar type II cells.", JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, vol. 129, no. 2, February 2003 (2003-02-01), pages 71 - 75, XP002464889, ISSN: 0171-5216 *
PFEIFER A M A ET AL: "Cooperation of c-raf-1 and c-myc protooncogenes in the neoplastic transformation of simian virus 40 large tumor antigen-immortalized human bronchial epithelial cells", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 86, no. 24, December 1989 (1989-12-01), pages 10075 - 10079, XP002147312, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
DE102007003670B4 (de) 2013-03-07
DE102007003670A1 (de) 2008-03-27
WO2008031430A2 (de) 2008-03-20

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