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WO2008031430A3 - Doubly transgenic non-human mammal - Google Patents

Doubly transgenic non-human mammal Download PDF

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Publication number
WO2008031430A3
WO2008031430A3 PCT/DE2007/001703 DE2007001703W WO2008031430A3 WO 2008031430 A3 WO2008031430 A3 WO 2008031430A3 DE 2007001703 W DE2007001703 W DE 2007001703W WO 2008031430 A3 WO2008031430 A3 WO 2008031430A3
Authority
WO
WIPO (PCT)
Prior art keywords
tumours
human
cell
transgenic non
human mammal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE2007/001703
Other languages
German (de)
French (fr)
Other versions
WO2008031430A2 (en
Inventor
Juergen Borlak
Roman Halter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fraunhofer Gesellschaft zur Foerderung der Angewandten Forschung eV
Original Assignee
Fraunhofer Gesellschaft zur Foerderung der Angewandten Forschung eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fraunhofer Gesellschaft zur Foerderung der Angewandten Forschung eV filed Critical Fraunhofer Gesellschaft zur Foerderung der Angewandten Forschung eV
Publication of WO2008031430A2 publication Critical patent/WO2008031430A2/en
Publication of WO2008031430A3 publication Critical patent/WO2008031430A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/82—Translation products from oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0331—Animal model for proliferative diseases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/02—Cells from transgenic animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Veterinary Medicine (AREA)
  • Environmental Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Oncology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention relates to transgenic non-human mammals carrying two transgenes in the genome thereof, to a method for producing said mammals and the use thereof, and cell tissue, cells and cell constituents thereof. The fields of application of the invention are the pharmaceutical industry and life sciences: biology, biochemistry, biotechnology, medicine, preferably the prevention and treatment of cancer, especially lung cancer. The aim of the invention is to provide an improved animal model which forms different types of tumours that can be compared with human tumours, especially lung tumours, and the metastases thereof. The aim of the invention is also to provide a non-human mammal with such improved characteristics and/or the cell tissue, cells and cell constituents thereof, and a method and means for the production of said mammal, and the use of the same. To this end, a doubly transgenic non-human mammal is provided with a genome containing a) a coding DNA sequence (1) which codes for a proto-oncogene and is operatively connected to at least one regulatory sequence, and b) a coding DNA sequence (2) which codes for an oncogene and is operatively connected to at least one regulatory sequence. The invention also relates to corresponding doubly transgenic tumour cell clones for use in the in vitro analysis of the pathogenesis of lung tumours and of substances or treatment methods for lung carcinomas.
PCT/DE2007/001703 2006-09-15 2007-09-17 Doubly transgenic non-human mammal Ceased WO2008031430A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE102006044013.7 2006-09-15
DE102006044013 2006-09-15
DE102007003670.3 2007-01-18
DE102007003670A DE102007003670B4 (en) 2006-09-15 2007-01-18 Double transgenic non-human mammal

Publications (2)

Publication Number Publication Date
WO2008031430A2 WO2008031430A2 (en) 2008-03-20
WO2008031430A3 true WO2008031430A3 (en) 2008-06-26

Family

ID=38896903

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2007/001703 Ceased WO2008031430A2 (en) 2006-09-15 2007-09-17 Doubly transgenic non-human mammal

Country Status (2)

Country Link
DE (1) DE102007003670B4 (en)
WO (1) WO2008031430A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999028453A2 (en) * 1997-11-28 1999-06-10 Rapp Ulf R Transgenic non-human mammals with an oncogenic mutant of raf gene 1

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999028453A2 (en) * 1997-11-28 1999-06-10 Rapp Ulf R Transgenic non-human mammals with an oncogenic mutant of raf gene 1

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ALEXANDER W S ET AL: "ONCOGENE COOPERATION IN LYMPHOCYTE TRANSFORMATION MALIGNANT CONVERSION OF E-MU-MYC TRANSGENIC PRE-B CELLS IN-VITRO IS ENHANCED BY V-H-RAS OR V-RAF BUT NOT V-ABL", MOLECULAR AND CELLULAR BIOLOGY, vol. 9, no. 1, 1989, pages 67 - 73, XP002464890, ISSN: 0270-7306 *
BLASI E ET AL: "SELECTIVE IMMORTALIZATION OF MURINE MACROPHAGES FROM FRESH BONE MARROW BY A RAF/MYC RECOMBINANT MURINE RETROVIRUS", NATURE, NATURE PUBLISHING GROUP, LONDON, GB, vol. 318, no. 6047, 19 December 1985 (1985-12-19), pages 667 - 670, XP001146446, ISSN: 0028-0836 *
EHRHARDT A ET AL: "Development of pulmonary bronchiolo-alveolar adenocarcinomas in transgenic mice overexpressing murine c-myc and epidermal growth factor in alveolar type II pneumocytes", BRITISH JOURNAL OF CANCER, vol. 84, no. 6, 23 March 2001 (2001-03-23), pages 813 - 818, XP002464888, ISSN: 0007-0920 *
EHRHARDT A ET AL: "Increased susceptibility to the tobacco carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in transgenic mice overexpressing c-myc and epidermal growth factor in alveolar type II cells.", JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, vol. 129, no. 2, February 2003 (2003-02-01), pages 71 - 75, XP002464889, ISSN: 0171-5216 *
PFEIFER A M A ET AL: "Cooperation of c-raf-1 and c-myc protooncogenes in the neoplastic transformation of simian virus 40 large tumor antigen-immortalized human bronchial epithelial cells", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 86, no. 24, December 1989 (1989-12-01), pages 10075 - 10079, XP002147312, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
DE102007003670A1 (en) 2008-03-27
DE102007003670B4 (en) 2013-03-07
WO2008031430A2 (en) 2008-03-20

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