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WO2005095576A2 - Incubateur a etage microscope pour une enveloppe d'eau (ou tout autre fluide) contenant du co2 a temperature commandee - Google Patents

Incubateur a etage microscope pour une enveloppe d'eau (ou tout autre fluide) contenant du co2 a temperature commandee Download PDF

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Publication number
WO2005095576A2
WO2005095576A2 PCT/IT2005/000161 IT2005000161W WO2005095576A2 WO 2005095576 A2 WO2005095576 A2 WO 2005095576A2 IT 2005000161 W IT2005000161 W IT 2005000161W WO 2005095576 A2 WO2005095576 A2 WO 2005095576A2
Authority
WO
WIPO (PCT)
Prior art keywords
fluid
controlled temperature
jacket
water
incubating chamber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IT2005/000161
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English (en)
Other versions
WO2005095576A3 (fr
Inventor
Luca Lanzaro
Sergio Caserta
Stefano Guido
Luigi Sabetta
Vincenzo Sibillo
Marino Simeone
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HIGH TECH CONSULTING Srl
Original Assignee
HIGH TECH CONSULTING Srl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HIGH TECH CONSULTING Srl filed Critical HIGH TECH CONSULTING Srl
Publication of WO2005095576A2 publication Critical patent/WO2005095576A2/fr
Publication of WO2005095576A3 publication Critical patent/WO2005095576A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/54—Constructional details, e.g. recesses, hinges hand portable
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14—Incubators; Climatic chambers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability

Definitions

  • the gas stream of humid air and CO 2 can be fed either in the entire box, resulting in potential damage of microscope mechanical parts and in water condensation on the box walls, or just in a small chamber placed on the microscope stage, thus resulting in water condensation on glass and plastic surfaces and medium evaporation from the sample under analysis, if the gas stream is fed at a temperature lower than the one inside the box surrounding the microscope.
  • it is quite laborious and time-consuming to remove the incubation set-up when it is not needed, and it is also very difficult to manage with the specimen once it is inside the box.
  • the main disadvantage is that it is heated through electrical resistance, a method that cannot provide the required thermal accuracy and stability upon ambient temperature changes.
  • chamber low thermal inertia and 2) what is controlled is chamber -metal temperature rather than sample temperature (thus causing a delay in the thermal controller response) ambient temperature changes affect the controlled temperature jeopardizing specimen vitality.
  • none of these systems is designed to be used with long
  • Controlled temperature water (or different fluid)-jacket CO 2 Microscope Stage Incubator (that in the following will be called incubating chamber) developed by High Tech Consulting s.r.l. is designed to maintain all the required environmental conditions suitable for cell cultures (or other biological specimens) right on the microscope stage, thus allowing prolonged observations of cell (or biological) events in any kind of inverted microscope (optical, confocal, electron, stereo and so on).
  • the incubating chamber is composed of two parts, a base and a cover, both of them being heated through inner controlled temperature water (or different fluid) circulation, provided by a water bath.
  • the incubating chamber could be composed of three parts, base, side walls and cover, if required due to the size of the sample vessels.
  • the incubating chamber has a thermal accuracy within 0.1 °C despite changes of ambient temperature, and temperature control is guaranteed by the joint action of a PID software controller and a thermocouple directly inserted inside the incubator.
  • the incubating chamber is made of aluminium (or alternatively of steel or any other metal). It has rectangular shape and dimensions that allow to place it on inverted microscope stages. The incubating chamber dimensions and shape may vary depending on the brand of the microscope and/or of the microscope stage.
  • holes are drawn to allow sample observation (i.e. Fig. 11 and Fig.13 -page 4 of the drawings) .
  • Such holes are always closed with glass (or Plexiglas or any other transparent non disruptive material) in the cover so that light can pass, whereas in the base they are left empty if immersion objectives have to be used to analyse the specimen, or again closed with glass (or Plexiglas or any other transparent non disruptive material) if long working distance objectives have to be used to analyse the specimen.
  • Shapes and dimensions of the holes in the central zone of the base of the incubating chamber are especially designed according to the type of vessel (containing the specimen) that has to be hosted. Holes in the cover are designed to correspond to holes in the base, so that light can pass through , thus allowing observation of the entire hosted sample.
  • the incubating chamber consists of two main parts: the base (that, in turn, can be composed of one or more pieces, without altering its operation principle) and the cover.
  • One or both of the two parts are partially hollow, to allow circulation of temperature-controlled water (or different fluid). Fluid circulation is usually provided by a water bath (or a cryostat water bath if a temperature below ambient temperature is required).
  • This heating (or cooling, when required) method allows, through heat conduction and heat radiation, to provide the desired thermal profile in the incubating chamber where specimens are hosted.
  • the thermal profile could reach values both above and below the ambient temperature, in the following we will refer only to the above ambient temperature application of the present invention, submeaning that cooling function is as possible as well .
  • the base comprises a central zone where specimen vessels are hosted and the desired gas stream is fed (as shown in Fig.8-page 2 of the drawings), and an inner zone where temperature controlled water (or other fluid) circulation takes place (as shown in Fig.9-page
  • the outside walls of the peripheral zone host three small holes (that don't have to be necessarily placed on the same base wall), one for water (or other fluid) inlet, one for water (or other fluid) outlet and a third hole for gas stream-inlet.
  • Water-inlet hole can be placed in the middle or in the side- part of the base wall.
  • the design and the position of the inner channels, where water (or other fluid) circulates would change without altering operation principle of the present invention.
  • the incubating chamber could also be internally totally empty, thus resulting in a different distribution of the internally circulating fluid (Fig.
  • controlled temperature water (or other fluid) circulation is as follows: it starts from the water bath, then it moves into the base of the incubating chamber through the channels drawn inside the inner zone of the base (i.e. Fig. 18-page 5 of the drawings). The water moves from the base to the cover of the incubating chamber(fro detail E to detail G-Fig. 34-page 8 of the drawings), again flowing through the channels drawn inside the cover (Fig. 24-page 5 of the drawings). From the cover (detail I-Fig.34- page 8 of the drawings), it then exits the chamber and goes back into the water bath, thus closing the water (or other fluid) circuit.
  • the controlled temperature water (or other fluid) circulation is as follows: coming from the water bath it moves into the base of the incubating chamber through the channels located inside the inner zone of the base. From the base it moves to the side walls of the incubating chamber, flowing through the channels inside the side walls (Fig. 5-page 2 of the drawings), it then flows into the channels inside the cover of the incubating chamber, and finally goes back to the water bath, thus closing the water (or other fluid) circuit. Controlled temperature water (or different fluid) circulation could change such above- mentioned direction without altering the operation principle and performances of the present invention. Connections between the water bath and the base of
  • each sample is placed in a recess drilled into the base of the incubating chamber. Shape and dimension of the recess vary according to the type of sample vessel (Petri dish, glass slide, Petri-dish for immersion objectives, glass slide, chamber slide and multiwell plate) to be accommodated.
  • the incubating chamber can be designed to host one or alternatively more than one (i.e. #four 35mm Petri-dishes, as shown in 13-page 4 of the drawings) of each sample vessel, or also different sample at the same time (i.e. #1 35mm Petri dish and #1 glass or chamber slide as shown in Fig. 31-pag.6 of the drawings).
  • Each recess is closed by a glass plate (or Plexiglas or any other transparent material) if the specimen has to be analysed by using long working distance objectives, or is left open if the specimen has to be analysed by using immersion objectives. Thickness of the base metal layer where the sample vessels are accommodated varies according to the necessary working distance between the sample and the objective.
  • Possible configurations include an incubating chamber with each recess closed by a glass plate (or Plexiglas or any other transparent material), an incubating chamber with each recess open and an incubating chamber with some recess closed by a glass plate (or Plexiglas or any other transparent material) and some other open.
  • Sample vessels usually have circular or rectangular shape.
  • each recess is made by drilling, from the bottom of the base, two or more concentric cylindrical holes.
  • the first cylindrical hole starts from the bottom of the base
  • the second one is to leave some room for the vessel lid, thus enabling gas exchange with the air stream circulating in the incubating chamber (Fig. 12-page 4 of the drawings).
  • the depth of the recess is designed to allow the correct working distances between the specimen and the objectives for observation under the microscope.
  • Analogous recesses but obviously with different shape, will be made when sample vessels have a rectangular form (Fig 30 and Fig. 32-page 6 of the drawings).
  • Different types of recess could be made in the same base to host different types of sample vessels.
  • Small vessels along the edge of the central zone of the incubating chamber base can be filled with water (meaning distilled water, or water and glycerine or any other liquid water solution) in order to minimise sample evaporation (Detail A-Page 3 of the drawings).
  • incubating chamber cover which can be made of aluminium, steel or any other metal
  • recesses are drilled whose shape and dimension correspond to the ones in the base where sample vessels are accommodated (Fig.11 -page 4 of the drawings).
  • Each recess in the cover can be closed by a glass, Plexiglas or any other transparent material plate.
  • Controlled temperature water (or other fluid) circulating inside the cover, as well as into the side walls, comes from the inner channels of the base.
  • cover and base are linked through a plastic tube, going from the water-outlet hole, placed in the external side of the base, to the water inlet hole on the top of the cover.
  • Water (or other fluid) passing into this tube moves from the base to the cover, heating both, and then, from the water-outlet hole placed on the top of the cover, returns into the water bath.
  • the base and the side walls and the cover are linked through two plastic tubes, one going from the water-outlet hole, placed in the external side of the base, to the water inlet hole placed in the external side of the side walls and the other going from the water-outlet hole, placed in the external side of the side walls to the water inlet hole on the top of the cover. Water (or other fluid) circulating through these tubes, goes from the base to the
  • Additional holes could be made, on the top of the cover or alternatively on the sides of the base or of the side walls, to allow perfusion of culture medium or other liquids.
  • the cover has overall dimensions so that it can be embedded, inserted or screwed to the base (or to the side walls) of the incubating chamber.
  • the desired gas stream that typically is composed of a mixture of air with 5% CO 2 but that could consist of different gases depending on the required conditions, is provided by gas sources and is a mixture of two or more gases, whose ratio is regulated trough gas flowmeters, connected to the gas sources by plastic (i.e. silicon or PNC) pipes.
  • Microbiological (or similar) filters can be placed between the gas sources and the gas flowmeters to remove impurity or soil and to make the gas stream sterile. After mixing, the gas stream goes into a bubbling column to be humidified (Fig. 38-Page 12 of the drawings).
  • the bubbling column consists of a glass cylinder filled with water.
  • the gas stream enters in this column and gurgles into the water, thus being humidified by mass transfer.
  • the gas stream flows into the incubating chamber, entering from a gas inlet-hole placed on one of the external walls of the base (detail D in Fig. 31-Pag.6 of the drawings) and outgoing from a gas outlet hole placed on the top of the cover (alternatively, a gas stream outlet hole can also be placed on an external side of the base of the incubating chamber).
  • the bubbling column itself is placed inside the water bath, so that the humidified gas stream enters into the incubating chamber at a temperature equal to or above the temperature in the incubating chamber.
  • the gas stream goes into a tube placed inside the channel drawn in the inner zone of the base or the cover of the incubating chamber, where temperature-controlled water (or different fluid) circulates (Fig. 31, detail C-page 6 of the drawings).
  • This pre-heating system is used to bring the gas stream at a temperature close to the one in the zone where the samples are accommodated.
  • the gas stream can go directly from the bubbling column to the zone where the samples are accommodated, without passing in the pre-heating circuit of the incubating chamber.
  • the gas stream is continuously fed into the incubating chamber, thus resulting in a continuous replacement of the gas surrounding the specimen, so that gas conditions in the incubating chamber never change from the desired values.
  • Temperature control is achieved by the joint action of a PID controller via software and a thermocouple directly inserted inside one of the sample vessels (filled with water, or with water and glycerine, or with any other liquid having a heat capacity close to the heat capacity of the medium in the sample under analysis.
  • temperature in each sample vessel is the same.
  • Sample temperature as measured by the thermocouple is read by a temperature meter communicating via serial (or USB) port with the PID controller software.
  • the PID controller software communicates with the water bath and regulates the temperature of the fluid inside the water bath based on the difference between sample temperature and the desired temperature (set- point temperature).
  • the PID controller software will act to increase the temperature of the water bath fluid if the thermocouple is reading a temperature
  • the PID controller software stops the feedback control and maintains the water bath temperature at the latest value before the incubating chamber was opened. The stopping of PID controller operation can be extended to a programmable duration after the incubating chamber has been closed again.
  • the present invention could also be used to control just one or two of the three parameters, i.e., temperature, humidity level and CO2 (or other gas) level, which usually need accurate control in long-term experiments involving biological specimens.
  • the present invention has been strictly tested, also comparing (referring to cell proliferation and sample medium evaporation) it with the bench incubator. Test results are shown in Fig. 33, 36 and 37-pages 7, 10 and 11 of the drawings
  • the incubating chamber can be composed of two, base and cover, or three, base, side walls and cover, depending on the height of the sample vessels; the fact that shape and dimensions of every part of the incubating can vary depending on the microscope it has to be fitted on; the fact that the number and the position of the holes where fluids, controlled temperature liquid fluid and gas stream, ingoing and outgoing to and from channels inner to the incubating chamber can vary as well as the direction of the circulation of the controlled temperature water or other fluid).
  • thermocouple is directly inserted in one of the sample- vessel filled with water, or with water and glycerine, or with any other liquid having a heat capacity close to the heat capacity of the medium in the sample under analysis but alternatively, it could also be inserted inside a small container (again filled with water, or with water and glycerine, or with any other liquid having a heat capacity close to the heat capacity of the medium in the sample under analysis) which is placed in close proximity with the sample vessels.
  • electrical resistance can be used to heat the bubbling column and the gas stream, instead of placing the bubbling column in the water bath.
  • Fig. 11 -Page 4 of the drawings could be preferred since it allows to host three different samples (we remember that the forth sample-lodgings is usually used to control the temperature). Otherwise when only one field of view has to be analysed, the embodiment shown in Fig. 31 -Page 6 of the drawings could be preferred.
  • Fig. 35-Page 9 of the drawings shows an embodiment designed to host a Multiwell plate. In the same way, depending on the type of objectives that have to be used, a specific embodiment could be preferred. Typical application of the present invention are time-lapse studies of cell events under microscope observations.

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  • Bioinformatics & Cheminformatics (AREA)
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  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
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  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Clinical Laboratory Science (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

L'invention concerne un incubateur à étage microscope pour une enveloppe d'eau contenant du CO2 ou tout autre fluide à température commandée, développé par High Tech Consulting s.r.l. , ledit incubateur étant conçu pour maintenir toutes les conditions environnementales pour la mise en culture des cellules (ou toute autre espèce biologique) sur l'étage du microscope, ce qui permet la réalisation d'observations prolongées d'événements cellulaires ou biologiques. Ledit incubateur comprend une chambre fabriquée en acier ou en aluminium ou tout autre matériau, et il présente des dimensions permettant de le placer verticalement sur l'étage microscope. Cela permet de maintenir de manière stable les conditions environnementales de la température et/ou de la teneur en humidité et/ou en CO2 (ou tout autre gaz) nécessaire à l'étude des phénomènes biologiques. Des conditions de température souhaitée sont fournies par la circulation de l'eau interne (ou d'autre fluide) et sont garanties grâce à l'action conjointe d'un dispositif de commande de logiciel PID, le thermocouple étant directement inséré à l'intérieur de l'incubateur. Les teneurs en humidité et en CO2 sont maintenues grâce à l'alimentation d'un flux d'air préconditionné regulé par des dispositifs de régulation de débits. Des orifices sont percés dans le fond de la chambre vers des échantillons hôtes, ce qui permet de les observer.
PCT/IT2005/000161 2004-04-02 2005-03-24 Incubateur a etage microscope pour une enveloppe d'eau (ou tout autre fluide) contenant du co2 a temperature commandee Ceased WO2005095576A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT000016A ITNA20040016A1 (it) 2004-04-02 2004-04-02 Incubatore a co2 da microscopio con circolazione interna di acqua o altro fluido a temperatura controllata
ITNA2004A000016 2004-04-02

Publications (2)

Publication Number Publication Date
WO2005095576A2 true WO2005095576A2 (fr) 2005-10-13
WO2005095576A3 WO2005095576A3 (fr) 2006-02-16

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IT (1) ITNA20040016A1 (fr)
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WO2006125980A3 (fr) * 2005-05-24 2007-01-18 Ivf Ltd Appareil de communication avec un marqueur de memoire et utilisation de celui-ci
WO2010133588A1 (fr) * 2009-05-20 2010-11-25 Institut Pasteur Dispositif pour cultiver in vitro des biofilms et ensemble d'observation non-invasive du développement de biofilms sur surface inerte ou sur surface vivante par microscopie confocale
EP1926011A3 (fr) * 2006-09-20 2011-03-30 Carl Zeiss MicroImaging GmbH Module et système de commande destinés à influencer des paramètres d'environnements d'échantillons d'un système d'incubation, procédé de commande d'un agencement de microscope et produit de programme informatique
DE102011054365A1 (de) * 2011-10-10 2013-04-11 DASGIP Information and Process Technology GmbH Biotechnologische Vorrichtung, Bioreaktorsystem mit mehreren biotechnologischen Vorrichtungen, Verfahren zum Temperieren eines Kultivierungsraumes in einer biotechnologischen Vorrichtung sowie Verfahren zum Temperieren von Kultivierungsräumen in einem Bioreaktorsystem
EP2980200A4 (fr) * 2013-03-25 2016-11-30 Hitachi Ltd Dispositif de culture de cellules, cuve de culture, et cuve de maintien
US9745547B2 (en) 2011-10-10 2017-08-29 Dasgip Information And Technology Gmbh Method for controlled operation of a biotechnological apparatus and bioreactor system
US10351812B2 (en) 2015-08-28 2019-07-16 Axion Biosystems, Inc. Device and system for creating and maintaining a localized environment for a cell culture plate
WO2020167959A1 (fr) * 2019-02-12 2020-08-20 University Of Washington Appareils, systèmes et procédés pour supports d'échantillons de microscope
CN112210497A (zh) * 2020-10-14 2021-01-12 度微检测技术(杭州)有限公司 结合led阵列的活细胞长时间孵育系统及光遗传学活细胞成像方法
CN112683904A (zh) * 2020-12-21 2021-04-20 中国科学院长春应用化学研究所 一种微生物与固体表面相互作用的原位表征装置及表征方法
CN113373046A (zh) * 2021-07-06 2021-09-10 济南惠凯诗生物科技有限公司 一种避免玻璃培养器皿发生爆裂的环保型支架辅助装置
EP4116400A1 (fr) * 2021-07-09 2023-01-11 Dominique Dumas Module d'incubation et de perfusion de matériaux biologiques
CN120084731A (zh) * 2025-05-06 2025-06-03 宁波礼达先导生物技术有限公司 一种自动化培养箱显微观察装置及使用方法

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IT202200002222A1 (it) 2022-02-08 2023-08-08 Univ Degli Studi Magna Graecia Di Catanzaro Piattaforma per screening di supporti cellulari statici e dinamici

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Cited By (20)

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US20090318751A1 (en) * 2004-05-24 2009-12-24 Ivf Limited Apparatus for Communicating with a Memory Tag and Use of the Same
EP2292332A3 (fr) * 2005-05-24 2011-05-25 Research Instruments Limited Appareil pour communiquer avec une étiquette mémoire et utilisation associée
WO2006125980A3 (fr) * 2005-05-24 2007-01-18 Ivf Ltd Appareil de communication avec un marqueur de memoire et utilisation de celui-ci
EP1926011A3 (fr) * 2006-09-20 2011-03-30 Carl Zeiss MicroImaging GmbH Module et système de commande destinés à influencer des paramètres d'environnements d'échantillons d'un système d'incubation, procédé de commande d'un agencement de microscope et produit de programme informatique
WO2010133588A1 (fr) * 2009-05-20 2010-11-25 Institut Pasteur Dispositif pour cultiver in vitro des biofilms et ensemble d'observation non-invasive du développement de biofilms sur surface inerte ou sur surface vivante par microscopie confocale
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