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WO2005095576A3 - Incubateur a etage microscope pour une enveloppe d'eau (ou tout autre fluide) contenant du co2 a temperature commandee - Google Patents

Incubateur a etage microscope pour une enveloppe d'eau (ou tout autre fluide) contenant du co2 a temperature commandee Download PDF

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Publication number
WO2005095576A3
WO2005095576A3 PCT/IT2005/000161 IT2005000161W WO2005095576A3 WO 2005095576 A3 WO2005095576 A3 WO 2005095576A3 IT 2005000161 W IT2005000161 W IT 2005000161W WO 2005095576 A3 WO2005095576 A3 WO 2005095576A3
Authority
WO
WIPO (PCT)
Prior art keywords
microscope stage
fluid
incubator
jacket
biological
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IT2005/000161
Other languages
English (en)
Other versions
WO2005095576A2 (fr
Inventor
Luca Lanzaro
Sergio Caserta
Stefano Guido
Luigi Sabetta
Vincenzo Sibillo
Marino Simeone
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HIGH TECH CONSULTING Srl
Original Assignee
HIGH TECH CONSULTING Srl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HIGH TECH CONSULTING Srl filed Critical HIGH TECH CONSULTING Srl
Publication of WO2005095576A2 publication Critical patent/WO2005095576A2/fr
Publication of WO2005095576A3 publication Critical patent/WO2005095576A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/54—Constructional details, e.g. recesses, hinges hand portable
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14—Incubators; Climatic chambers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Cell Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

L'invention concerne un incubateur à étage microscope pour une enveloppe d'eau contenant du CO2 ou tout autre fluide à température commandée, développé par High Tech Consulting s.r.l. , ledit incubateur étant conçu pour maintenir toutes les conditions environnementales pour la mise en culture des cellules (ou toute autre espèce biologique) sur l'étage du microscope, ce qui permet la réalisation d'observations prolongées d'événements cellulaires ou biologiques. Ledit incubateur comprend une chambre fabriquée en acier ou en aluminium ou tout autre matériau, et il présente des dimensions permettant de le placer verticalement sur l'étage microscope. Cela permet de maintenir de manière stable les conditions environnementales de la température et/ou de la teneur en humidité et/ou en CO2 (ou tout autre gaz) nécessaire à l'étude des phénomènes biologiques. Des conditions de température souhaitée sont fournies par la circulation de l'eau interne (ou d'autre fluide) et sont garanties grâce à l'action conjointe d'un dispositif de commande de logiciel PID, le thermocouple étant directement inséré à l'intérieur de l'incubateur. Les teneurs en humidité et en CO2 sont maintenues grâce à l'alimentation d'un flux d'air préconditionné regulé par des dispositifs de régulation de débits. Des orifices sont percés dans le fond de la chambre vers des échantillons hôtes, ce qui permet de les observer.
PCT/IT2005/000161 2004-04-02 2005-03-24 Incubateur a etage microscope pour une enveloppe d'eau (ou tout autre fluide) contenant du co2 a temperature commandee Ceased WO2005095576A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT000016A ITNA20040016A1 (it) 2004-04-02 2004-04-02 Incubatore a co2 da microscopio con circolazione interna di acqua o altro fluido a temperatura controllata
ITNA2004A000016 2004-04-02

Publications (2)

Publication Number Publication Date
WO2005095576A2 WO2005095576A2 (fr) 2005-10-13
WO2005095576A3 true WO2005095576A3 (fr) 2006-02-16

Family

ID=34968351

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IT2005/000161 Ceased WO2005095576A2 (fr) 2004-04-02 2005-03-24 Incubateur a etage microscope pour une enveloppe d'eau (ou tout autre fluide) contenant du co2 a temperature commandee

Country Status (2)

Country Link
IT (1) ITNA20040016A1 (fr)
WO (1) WO2005095576A2 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1726362A1 (fr) * 2005-05-24 2006-11-29 IVF Limited Dispositive pour la communication avec une puce mémoire et avec une surface pour contrôler la témperature
DE102006044091A1 (de) * 2006-09-20 2008-04-03 Carl Zeiss Microimaging Gmbh Steuermodul und Steuersystem zur Beeinflussung von Probenumgebungsparametern eines Inkubationssystems, Verfahren zur Steuerung einer Mikroskopanordnung und Computerprogrammprodukt
FR2945818A1 (fr) * 2009-05-20 2010-11-26 Pasteur Institut Dispositif pour cultiver in vitro des biofilms et ensemble d'observation non-invasive du developpement de biofilms sur surface inerte ou sur surface vivante par microscopie confocale
CN103998596B (zh) 2011-10-10 2016-05-18 德国达斯其普信息与程序技术有限公司 受控运行生物技术装置的方法以及生物反应器系统
DE102011054365B4 (de) * 2011-10-10 2014-01-02 DASGIP Information and Process Technology GmbH Biotechnologische Vorrichtung, Bioreaktorsystem mit mehreren biotechnologischen Vorrichtungen, Verfahren zum Temperieren eines Kultivierungsraumes in einer biotechnologischen Vorrichtung sowie Verfahren zum Temperieren von Kultivierungsräumen in einem Bioreaktorsystem
WO2014155500A1 (fr) * 2013-03-25 2014-10-02 株式会社日立製作所 Dispositif de culture de cellules, cuve de culture, et cuve de maintien
US10351812B2 (en) 2015-08-28 2019-07-16 Axion Biosystems, Inc. Device and system for creating and maintaining a localized environment for a cell culture plate
US20220050280A1 (en) * 2019-02-12 2022-02-17 University Of Washington Apparatuses, systems and methods for microscope sample holders
CN112210497A (zh) * 2020-10-14 2021-01-12 度微检测技术(杭州)有限公司 结合led阵列的活细胞长时间孵育系统及光遗传学活细胞成像方法
CN112683904B (zh) * 2020-12-21 2022-04-26 中国科学院长春应用化学研究所 一种微生物与固体表面相互作用的原位表征装置及表征方法
CN113373046A (zh) * 2021-07-06 2021-09-10 济南惠凯诗生物科技有限公司 一种避免玻璃培养器皿发生爆裂的环保型支架辅助装置
EP4116400A1 (fr) 2021-07-09 2023-01-11 Dominique Dumas Module d'incubation et de perfusion de matériaux biologiques
IT202200002222A1 (it) 2022-02-08 2023-08-08 Univ Degli Studi Magna Graecia Di Catanzaro Piattaforma per screening di supporti cellulari statici e dinamici
CN120084731A (zh) * 2025-05-06 2025-06-03 宁波礼达先导生物技术有限公司 一种自动化培养箱显微观察装置及使用方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4301252A (en) * 1980-04-04 1981-11-17 Baker Fraser L Controlled environment incubator for light microscopy
US4629862A (en) * 1984-03-28 1986-12-16 Olympus Optical Company Ltd. Sample heater for use in microscopes
US20010046702A1 (en) * 1999-04-27 2001-11-29 Schembri Carol T. Devices for performing array hybridization assays and methods of using the same
US6365367B1 (en) * 1999-12-06 2002-04-02 Cellomics, Inc. Environmental chamber for the analysis of live cells

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4301252A (en) * 1980-04-04 1981-11-17 Baker Fraser L Controlled environment incubator for light microscopy
US4629862A (en) * 1984-03-28 1986-12-16 Olympus Optical Company Ltd. Sample heater for use in microscopes
US20010046702A1 (en) * 1999-04-27 2001-11-29 Schembri Carol T. Devices for performing array hybridization assays and methods of using the same
US6365367B1 (en) * 1999-12-06 2002-04-02 Cellomics, Inc. Environmental chamber for the analysis of live cells

Also Published As

Publication number Publication date
WO2005095576A2 (fr) 2005-10-13
ITNA20040016A1 (it) 2004-07-02

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