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WO2004011938A3 - Procede et dispositif pour le criblage de molecules dans des cellules - Google Patents

Procede et dispositif pour le criblage de molecules dans des cellules Download PDF

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Publication number
WO2004011938A3
WO2004011938A3 PCT/FR2003/002298 FR0302298W WO2004011938A3 WO 2004011938 A3 WO2004011938 A3 WO 2004011938A3 FR 0302298 W FR0302298 W FR 0302298W WO 2004011938 A3 WO2004011938 A3 WO 2004011938A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
reagent
support
cells
aqueous
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR2003/002298
Other languages
English (en)
Other versions
WO2004011938A2 (fr
WO2004011938B1 (fr
Inventor
Beatrice Schaack
Francois Chatelain
Brigitte Fouque
Alexandra Fuchs
Yves Fouillet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Original Assignee
Commissariat a lEnergie Atomique CEA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Commissariat a lEnergie Atomique CEA filed Critical Commissariat a lEnergie Atomique CEA
Priority to JP2004523860A priority Critical patent/JP2005533509A/ja
Priority to CA002492933A priority patent/CA2492933A1/fr
Priority to EP03750828A priority patent/EP1525472A2/fr
Priority to AU2003269041A priority patent/AU2003269041A1/en
Publication of WO2004011938A2 publication Critical patent/WO2004011938A2/fr
Publication of WO2004011938A3 publication Critical patent/WO2004011938A3/fr
Publication of WO2004011938B1 publication Critical patent/WO2004011938B1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5032—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on intercellular interactions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5058—Neurological cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00364—Pipettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00364—Pipettes
    • B01J2219/00367—Pipettes capillary
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00378—Piezoelectric or ink jet dispensers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00646—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports
    • B01J2219/0065—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports by the use of liquid beads
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • G01N2035/1046—Levitated, suspended drops

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un procédé de mise en réaction d'un réactif R avec une cellule C, dans lequel :- la cellule C est déposée sur un support S comportant une surface sensiblement plane, sous forme d'une goutte aqueuse sur ladite surface,- la surface plane du support S sur laquelle a été déposée la goutte aqueuse contenant la cellule C est recouverte par un film de séparation F, permettant le passage des gaz et empêchant l'évaporation des gouttes aqueuses déposées sur le support S, F étant non miscible avec le réactif R,- la réaction entre le réactif R et la cellule C est déclenchée par l'introduction du réactif R dans la goutte aqueuse contenant la cellule C.L'invention concerne également un dispositif pour la mise en uvre de ce procédé et ses utilisations.
PCT/FR2003/002298 2002-07-23 2003-07-21 Procede et dispositif pour le criblage de molecules dans des cellules Ceased WO2004011938A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2004523860A JP2005533509A (ja) 2002-07-23 2003-07-21 細胞中の分子をスクリーニングするための方法及び装置
CA002492933A CA2492933A1 (fr) 2002-07-23 2003-07-21 Procede et dispositif pour le criblage de molecules dans des cellules
EP03750828A EP1525472A2 (fr) 2002-07-23 2003-07-21 Procede et dispositif pour le criblage de molecules dans des cellules
AU2003269041A AU2003269041A1 (en) 2002-07-23 2003-07-21 Method and device for screening molecules in cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0209326A FR2842747B1 (fr) 2002-07-23 2002-07-23 Procede et dispositif pour le criblage de molecules dans des cellules
FR0209326 2002-07-23

Publications (3)

Publication Number Publication Date
WO2004011938A2 WO2004011938A2 (fr) 2004-02-05
WO2004011938A3 true WO2004011938A3 (fr) 2004-08-05
WO2004011938B1 WO2004011938B1 (fr) 2004-09-16

Family

ID=30011408

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2003/002298 Ceased WO2004011938A2 (fr) 2002-07-23 2003-07-21 Procede et dispositif pour le criblage de molecules dans des cellules

Country Status (6)

Country Link
EP (1) EP1525472A2 (fr)
JP (1) JP2005533509A (fr)
AU (1) AU2003269041A1 (fr)
CA (1) CA2492933A1 (fr)
FR (1) FR2842747B1 (fr)
WO (1) WO2004011938A2 (fr)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2857451B1 (fr) * 2003-07-11 2005-09-30 Commissariat Energie Atomique Procede et dispositif pour l'analyse de milieux reactionnels vivants
US20090215192A1 (en) * 2004-05-27 2009-08-27 Stratos Biosystems, Llc Solid-phase affinity-based method for preparing and manipulating an analyte-containing solution
DK1859330T3 (da) 2005-01-28 2012-10-15 Univ Duke Apparater og fremgangsmåder til håndtering af små dråber på et trykt kredsløbskort
US7951584B2 (en) 2005-03-30 2011-05-31 National University Corporation Nagoya University Method for production of biological organic material and culture vessel
JPWO2007063898A1 (ja) * 2005-11-29 2009-05-07 国立大学法人 東京大学 タンパク質結晶化方法及び装置並びにタンパク質結晶化処理装置
US9476856B2 (en) 2006-04-13 2016-10-25 Advanced Liquid Logic, Inc. Droplet-based affinity assays
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US9675972B2 (en) 2006-05-09 2017-06-13 Advanced Liquid Logic, Inc. Method of concentrating beads in a droplet
EP2570812B1 (fr) 2007-02-09 2018-07-18 Advanced Liquid Logic, Inc. Procédé d'assemblage d'un dispositif actionneurs de gouttelettes
WO2011084703A2 (fr) 2009-12-21 2011-07-14 Advanced Liquid Logic, Inc. Analyses d'enzymes sur un diffuseur à gouttelettes
US8702938B2 (en) 2007-09-04 2014-04-22 Advanced Liquid Logic, Inc. Droplet actuator with improved top substrate
US9631244B2 (en) 2007-10-17 2017-04-25 Advanced Liquid Logic, Inc. Reagent storage on a droplet actuator
CA2709928A1 (fr) 2007-12-23 2009-07-09 Advanced Liquid Logic, Inc. Configurations d'actionneur de formation de gouttelettes, et procedes de realisation d'operations de formation de gouttelettes
US8852952B2 (en) 2008-05-03 2014-10-07 Advanced Liquid Logic, Inc. Method of loading a droplet actuator
US8926065B2 (en) 2009-08-14 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
US9091649B2 (en) 2009-11-06 2015-07-28 Advanced Liquid Logic, Inc. Integrated droplet actuator for gel; electrophoresis and molecular analysis
WO2011142117A1 (fr) 2010-05-11 2011-11-17 パナソニック株式会社 Substrat de culture de cellules et procédé de culture de cellules utilisant celui-ci
CA2833897C (fr) 2011-05-09 2020-05-19 Advanced Liquid Logic, Inc. Retroaction microfluidique utilisant une detection d'impedance
US9513253B2 (en) 2011-07-11 2016-12-06 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based enzymatic assays
US9446404B2 (en) 2011-07-25 2016-09-20 Advanced Liquid Logic, Inc. Droplet actuator apparatus and system
US10731199B2 (en) 2011-11-21 2020-08-04 Advanced Liquid Logic, Inc. Glucose-6-phosphate dehydrogenase assays
BR112014032727B1 (pt) 2012-06-27 2021-12-14 Illumina France Método e sistema para realizar operações de gotícula em uma gotícula em um atuador de gotículas para redução da formação de bolhas
US10072241B2 (en) 2013-03-13 2018-09-11 Innovative Surface Technologies, Inc. Conical devices for three-dimensional aggregate(s) of eukaryotic cells
CN108472647B (zh) 2015-10-16 2021-06-29 牛津大学科技创新有限公司 微流体布置
GB201614150D0 (en) 2016-08-18 2016-10-05 Univ Oxford Innovation Ltd Microfluidic arrangements
GB201518392D0 (en) * 2015-10-16 2015-12-02 Isis Innovation Microfluidic arrangements
US20230001416A1 (en) * 2019-11-06 2023-01-05 Korea University Research And Business Foundation Droplet deformation-based method of transferring material into cells and chip for same
JP7135049B2 (ja) * 2020-10-12 2022-09-12 エコール ポリテクニック サンプルを含むマイクロ液滴を取り扱うための方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997039328A1 (fr) * 1996-04-12 1997-10-23 Australian Biomedical Corporation Ltd. Procede et appareil permettant de traiter des echantillons de cellule humaine ou animale
WO1998041869A1 (fr) * 1997-03-18 1998-09-24 Chromaxome Corporation Methodes de tri de composes au moyen de cellules encapsulees
WO1999055827A1 (fr) * 1998-04-27 1999-11-04 Amersham Pharmacia Biotech Uk Ltd. Appareil micro-usine pour dosages a base de cellules

Family Cites Families (1)

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Publication number Priority date Publication date Assignee Title
FR2857451B1 (fr) * 2003-07-11 2005-09-30 Commissariat Energie Atomique Procede et dispositif pour l'analyse de milieux reactionnels vivants

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
WO1997039328A1 (fr) * 1996-04-12 1997-10-23 Australian Biomedical Corporation Ltd. Procede et appareil permettant de traiter des echantillons de cellule humaine ou animale
WO1998041869A1 (fr) * 1997-03-18 1998-09-24 Chromaxome Corporation Methodes de tri de composes au moyen de cellules encapsulees
WO1999055827A1 (fr) * 1998-04-27 1999-11-04 Amersham Pharmacia Biotech Uk Ltd. Appareil micro-usine pour dosages a base de cellules

Non-Patent Citations (3)

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Title
"Toxicity bioassay of heavy metals in water using Tetrahymena pyriformis", WATER RESEARCH 1973, vol. 7, no. 7, 1973, pages 951 - 961, XP008026543 *
See also references of EP1525472A2 *
ZIAUDDIN JUNAID ET AL: "Microarrays of cells expressing defined cDNAs.", NATURE (LONDON), vol. 411, no. 6833, 2001, pages 107 - 110, XP002246363, ISSN: 0028-0836 *

Also Published As

Publication number Publication date
FR2842747B1 (fr) 2004-10-15
WO2004011938A2 (fr) 2004-02-05
FR2842747A1 (fr) 2004-01-30
AU2003269041A1 (en) 2004-02-16
AU2003269041A8 (en) 2004-02-16
JP2005533509A (ja) 2005-11-10
WO2004011938B1 (fr) 2004-09-16
EP1525472A2 (fr) 2005-04-27
CA2492933A1 (fr) 2004-02-05

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