[go: up one dir, main page]

AU2003269041A1 - Method and device for screening molecules in cells - Google Patents

Method and device for screening molecules in cells

Info

Publication number
AU2003269041A1
AU2003269041A1 AU2003269041A AU2003269041A AU2003269041A1 AU 2003269041 A1 AU2003269041 A1 AU 2003269041A1 AU 2003269041 A AU2003269041 A AU 2003269041A AU 2003269041 A AU2003269041 A AU 2003269041A AU 2003269041 A1 AU2003269041 A1 AU 2003269041A1
Authority
AU
Australia
Prior art keywords
cells
screening molecules
screening
molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003269041A
Other versions
AU2003269041A8 (en
Inventor
Francois Chatelain
Yves Fouillet
Brigitte Fouque
Alexandra Fuchs
Beatrice Schaack
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Original Assignee
Commissariat a lEnergie Atomique CEA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Commissariat a lEnergie Atomique CEA filed Critical Commissariat a lEnergie Atomique CEA
Publication of AU2003269041A1 publication Critical patent/AU2003269041A1/en
Publication of AU2003269041A8 publication Critical patent/AU2003269041A8/en
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5032—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on intercellular interactions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5058—Neurological cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00364—Pipettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00364—Pipettes
    • B01J2219/00367—Pipettes capillary
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00378—Piezoelectric or ink jet dispensers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00646—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports
    • B01J2219/0065—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports by the use of liquid beads
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • G01N2035/1046—Levitated, suspended drops

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2003269041A 2002-07-23 2003-07-21 Method and device for screening molecules in cells Abandoned AU2003269041A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0209326A FR2842747B1 (en) 2002-07-23 2002-07-23 METHOD AND DEVICE FOR SCREENING MOLECULES IN CELLS
FR0209326 2002-07-23
PCT/FR2003/002298 WO2004011938A2 (en) 2002-07-23 2003-07-21 Method and device for screening molecules in cells

Publications (2)

Publication Number Publication Date
AU2003269041A1 true AU2003269041A1 (en) 2004-02-16
AU2003269041A8 AU2003269041A8 (en) 2004-02-16

Family

ID=30011408

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003269041A Abandoned AU2003269041A1 (en) 2002-07-23 2003-07-21 Method and device for screening molecules in cells

Country Status (6)

Country Link
EP (1) EP1525472A2 (en)
JP (1) JP2005533509A (en)
AU (1) AU2003269041A1 (en)
CA (1) CA2492933A1 (en)
FR (1) FR2842747B1 (en)
WO (1) WO2004011938A2 (en)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2857451B1 (en) * 2003-07-11 2005-09-30 Commissariat Energie Atomique METHOD AND DEVICE FOR ANALYSIS OF LIVE REACTION ENVIRONMENTS
US20090215192A1 (en) * 2004-05-27 2009-08-27 Stratos Biosystems, Llc Solid-phase affinity-based method for preparing and manipulating an analyte-containing solution
DK1859330T3 (en) 2005-01-28 2012-10-15 Univ Duke DEVICES AND METHODS OF HANDLING SMALL DROPS ON A PRINTED CIRCUIT CARD
US7951584B2 (en) 2005-03-30 2011-05-31 National University Corporation Nagoya University Method for production of biological organic material and culture vessel
JPWO2007063898A1 (en) * 2005-11-29 2009-05-07 国立大学法人 東京大学 Protein crystallization method and apparatus, and protein crystallization treatment apparatus
US9476856B2 (en) 2006-04-13 2016-10-25 Advanced Liquid Logic, Inc. Droplet-based affinity assays
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US9675972B2 (en) 2006-05-09 2017-06-13 Advanced Liquid Logic, Inc. Method of concentrating beads in a droplet
EP2570812B1 (en) 2007-02-09 2018-07-18 Advanced Liquid Logic, Inc. Method of assembling a droplet actuator assembly
WO2011084703A2 (en) 2009-12-21 2011-07-14 Advanced Liquid Logic, Inc. Enzyme assays on a droplet actuator
US8702938B2 (en) 2007-09-04 2014-04-22 Advanced Liquid Logic, Inc. Droplet actuator with improved top substrate
US9631244B2 (en) 2007-10-17 2017-04-25 Advanced Liquid Logic, Inc. Reagent storage on a droplet actuator
CA2709928A1 (en) 2007-12-23 2009-07-09 Advanced Liquid Logic, Inc. Droplet actuator configurations and methods of conducting droplet operations
US8852952B2 (en) 2008-05-03 2014-10-07 Advanced Liquid Logic, Inc. Method of loading a droplet actuator
US8926065B2 (en) 2009-08-14 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
US9091649B2 (en) 2009-11-06 2015-07-28 Advanced Liquid Logic, Inc. Integrated droplet actuator for gel; electrophoresis and molecular analysis
WO2011142117A1 (en) 2010-05-11 2011-11-17 パナソニック株式会社 Cell culture substrate and cell culture method using same
CA2833897C (en) 2011-05-09 2020-05-19 Advanced Liquid Logic, Inc. Microfluidic feedback using impedance detection
US9513253B2 (en) 2011-07-11 2016-12-06 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based enzymatic assays
US9446404B2 (en) 2011-07-25 2016-09-20 Advanced Liquid Logic, Inc. Droplet actuator apparatus and system
US10731199B2 (en) 2011-11-21 2020-08-04 Advanced Liquid Logic, Inc. Glucose-6-phosphate dehydrogenase assays
BR112014032727B1 (en) 2012-06-27 2021-12-14 Illumina France METHOD AND SYSTEM FOR PERFORMING DROP OPERATIONS ON A DROPOLE ON A DROP ACTUATOR TO REDUCE BUBBLE FORMATION
US10072241B2 (en) 2013-03-13 2018-09-11 Innovative Surface Technologies, Inc. Conical devices for three-dimensional aggregate(s) of eukaryotic cells
CN108472647B (en) 2015-10-16 2021-06-29 牛津大学科技创新有限公司 microfluidic arrangement
GB201614150D0 (en) 2016-08-18 2016-10-05 Univ Oxford Innovation Ltd Microfluidic arrangements
GB201518392D0 (en) * 2015-10-16 2015-12-02 Isis Innovation Microfluidic arrangements
US20230001416A1 (en) * 2019-11-06 2023-01-05 Korea University Research And Business Foundation Droplet deformation-based method of transferring material into cells and chip for same
JP7135049B2 (en) * 2020-10-12 2022-09-12 エコール ポリテクニック Methods for handling microdroplets containing samples

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPN923596A0 (en) * 1996-04-12 1996-05-09 Australian Biomedical Corporation Limited Method and apparatus for treatment of human or animal cell samples
CA2284066A1 (en) * 1997-03-18 1998-09-24 Chromaxome Corporation Methods for screening compounds using encapsulated cells
GB9808836D0 (en) * 1998-04-27 1998-06-24 Amersham Pharm Biotech Uk Ltd Microfabricated apparatus for cell based assays
FR2857451B1 (en) * 2003-07-11 2005-09-30 Commissariat Energie Atomique METHOD AND DEVICE FOR ANALYSIS OF LIVE REACTION ENVIRONMENTS

Also Published As

Publication number Publication date
FR2842747B1 (en) 2004-10-15
WO2004011938A2 (en) 2004-02-05
FR2842747A1 (en) 2004-01-30
WO2004011938A3 (en) 2004-08-05
AU2003269041A8 (en) 2004-02-16
JP2005533509A (en) 2005-11-10
WO2004011938B1 (en) 2004-09-16
EP1525472A2 (en) 2005-04-27
CA2492933A1 (en) 2004-02-05

Similar Documents

Publication Publication Date Title
AU2003269041A1 (en) Method and device for screening molecules in cells
AU2003259289A1 (en) Apparatus and methods for binding molecules and cells
EP1552002A4 (en) Aptamer-toxin molecules and methods for using same
EP1610135B8 (en) Test device and test method
AU2003273044A1 (en) Method and device for roaming-connection in global network
AU2003303361A1 (en) Analysis apparatus and method
AU2003233956A1 (en) Method and apparatus for improved cell detection
AU2003301186A1 (en) Method and apparatus for following cells
AU2003234459A1 (en) Device and method for analytical cell imaging
AU2003248950A1 (en) Liquid sampler and method
AU2003231846A1 (en) Apparatus and methods for detecting a microbe in a sample
AU2003256710A1 (en) Auto-stimulating cells and method for making and using the same
AU2003262986A1 (en) Method for forming conductive material in opening and structure regarding same
AU2002952181A0 (en) Electrolysis process and cell for use in same
AU2003265815A1 (en) Parametric array modulation and processing method
AU2002951034A0 (en) Testing process and apparatus
AU2003258459A1 (en) Device and method for inspecting material
AU2002363921A1 (en) Method and apparatus for distortion analysis in nonlinear circuits
AU2003288387A1 (en) Droplet - deposition related methods and apparatus
AU2002357890A1 (en) Method and apparatus for frame delimitation in arq systems
AU2002347417A1 (en) Space-dyeing method and apparatus
AU2003284060A1 (en) Methods and apparatus for identifying compounds in a sample
AU2003216766A1 (en) Method and device for use in micturition studies
AU2003237758A1 (en) Method and device for producing biogas
AU2003281792A1 (en) Method and apparatus for accessing multiple vector elements in parallel

Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase