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WO2003012389A3 - Verfahren zur analyse von makromolekülen, analysevorrichtung und verfahren zur herstellung einer analysevorrichtung - Google Patents

Verfahren zur analyse von makromolekülen, analysevorrichtung und verfahren zur herstellung einer analysevorrichtung Download PDF

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Publication number
WO2003012389A3
WO2003012389A3 PCT/EP2002/007644 EP0207644W WO03012389A3 WO 2003012389 A3 WO2003012389 A3 WO 2003012389A3 EP 0207644 W EP0207644 W EP 0207644W WO 03012389 A3 WO03012389 A3 WO 03012389A3
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WO
WIPO (PCT)
Prior art keywords
drop
macromolecules
liquid
spots
microarray
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Ceased
Application number
PCT/EP2002/007644
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English (en)
French (fr)
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WO2003012389A2 (de
Inventor
Juergen Scriba
Christoph Gauer
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Advalytix AG
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Advalytix AG
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Filing date
Publication date
Application filed by Advalytix AG filed Critical Advalytix AG
Priority to EP02764659A priority Critical patent/EP1412533A2/de
Priority to AU2002328867A priority patent/AU2002328867A1/en
Publication of WO2003012389A2 publication Critical patent/WO2003012389A2/de
Publication of WO2003012389A3 publication Critical patent/WO2003012389A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B01J2219/00533—Sheets essentially rectangular
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
    • B01L2400/0436—Moving fluids with specific forces or mechanical means specific forces vibrational forces acoustic forces, e.g. surface acoustic waves [SAW]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
    • B01L2400/0439—Moving fluids with specific forces or mechanical means specific forces vibrational forces ultrasonic vibrations, vibrating piezo elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0493—Specific techniques used
    • B01L2400/0496—Travelling waves, e.g. in combination with electrical or acoustic forces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)

Abstract

Die Erfindung betrifft Verfahren zur Analyse von Makromolekülen mit Hilfe eines Microarrays, das aus mehreren Spots in bekannter Anordnung besteht, die eine Vielzahl von ersten zumindest teilweise unterschiedlichen Makromolekülen umfassen, wobei sich das Microarray auf einer Festkörperoberfläche, vorzugsweise eines Chips, befindet, bei dem zumindest ein Flüssigkeitstropfen mit Makromolekülen auf die Festkörperoberfläche gebracht wird, wobei die Flüssigkeitsmenge so klein ist, dass im Wesentlichen nur ein oder eine Gruppe von wenigen Spots des Microarrays mit dem Flüssigkeitstropfen in Berührung kommt, und nach Ablauf einer vorbestimmten Zeit der Flüssigkeitstropfen mit Hilfe des Impulsübertrages einer Oberflächenschallwelle zu einem nächsten Spot oder einer weiteren Gruppe weniger Spots bewegt wird. Die mit dem Flüssigkeitstropfen in Berührung gekommenen Spots werden daraufhin untersucht, ob eine Reaktion mit dem Material des Flüssigkeitstropfens stattgefunden hat. Die Erfindung betrifft weiterhin ein Verfahren zur Herstellung einer Analysevorrichtung für Makromoleküle, bei dem zumindest ein Flüssigkeitstropfen mit zumindest einer Art von Makromolekülen auf eine Festkörperoberfläche, vorzugsweise eines Chips, aufgebracht wird, wobei die Makromoleküle eine aktive Gruppe aufweisen, die bei Änderung eines entsprechenden Parameters aktiviert wird, um eine Bindung an die Oberfläche oder bereits gebundenen Molekülen zu ermöglichen. Der Tropfen wird mit Hilfe des Impulsübertrages einer oder mehrerer Oberflächenschallwellen an einen gewünschten Ort auf der Oberfläche bewegt. Der äussere Parameter wird homogen und nicht lokal verändert, so dass eine Bindung der Makromoleküle ermöglicht wird, so dass der Spot eines Microarrays gebildet wird, in dem Makromoleküle aus dem Flüssigkeitstropfen an der Oberfläche gebunden sind. Schliesslich betrifft die Erfindung eine Analysevorrichtung zur Durchführung der erfindungsgemässen Analyseverfahren.
PCT/EP2002/007644 2001-07-24 2002-07-09 Verfahren zur analyse von makromolekülen, analysevorrichtung und verfahren zur herstellung einer analysevorrichtung Ceased WO2003012389A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP02764659A EP1412533A2 (de) 2001-07-24 2002-07-09 Verfahren zur analyse von makromolekülen, analysevorrichtung und verfahren zur herstellung einer analysevorrichtung
AU2002328867A AU2002328867A1 (en) 2001-07-24 2002-07-09 Method for analysing macromolecules, analysis device and a method for producing an analysis device

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10136008.8 2001-07-24
DE10136008A DE10136008B4 (de) 2001-07-24 2001-07-24 Verfahren zur Analyse von Makromolekülen und Verfahren zur Herstellung einer Analysevorrichtung

Publications (2)

Publication Number Publication Date
WO2003012389A2 WO2003012389A2 (de) 2003-02-13
WO2003012389A3 true WO2003012389A3 (de) 2003-07-17

Family

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Family Applications (1)

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PCT/EP2002/007644 Ceased WO2003012389A2 (de) 2001-07-24 2002-07-09 Verfahren zur analyse von makromolekülen, analysevorrichtung und verfahren zur herstellung einer analysevorrichtung

Country Status (4)

Country Link
EP (1) EP1412533A2 (de)
AU (1) AU2002328867A1 (de)
DE (1) DE10136008B4 (de)
WO (1) WO2003012389A2 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10328624A1 (de) * 2003-06-25 2005-01-20 Advalytix Ag Verfahren zur Hybridisierung
DE102004037348A1 (de) * 2004-08-02 2006-03-16 Infineon Technologies Ag Fluid-Transport-Vorrichtung, Sensor-Anordnung, Fluid-Misch-Vorrichtung und Verfahren zum Herstellen einer Fluid-Transport-Vorrichtung
DE102004051394B4 (de) * 2004-10-21 2006-08-17 Advalytix Ag Verfahren zur Bewegung von kleinen Flüssigkeitsmengen in Mikrokanälen und Mikrokanalsystem
DE102005000835B3 (de) 2005-01-05 2006-09-07 Advalytix Ag Verfahren und Vorrichtung zur Dosierung kleiner Flüssigkeitsmengen
US8062904B2 (en) 2005-01-05 2011-11-22 Beckman Coulter, Inc. Method and device for dosing and mixing small amounts of liquid
DE102005056639A1 (de) * 2005-11-28 2007-06-06 Advalytix Ag Verfahren, Vorrichtung und Kit zur Untersuchung von Makromolekülen in einer Probe
DE102006056694B4 (de) * 2006-11-30 2010-08-05 Advalytix Ag Verfahren zum Durchführen einer enzymatischen Reaktion
WO2008080531A2 (de) * 2007-01-05 2008-07-10 Advalytix Ag Verfahren, vorrichtung und kit zur untersuchung einer flüssigkeitsprobe
EP2194381B1 (de) * 2008-12-03 2015-12-02 Roche Diagnostics GmbH Testelement mit kombinierter Kontroll- und Kalibrationszone
JP2015512766A (ja) * 2012-01-31 2015-04-30 ザ・ペン・ステート・リサーチ・ファンデーション 可変定常的表面弾性波を用いたマイクロ流体操作及び粒子の分類

Citations (4)

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WO1994027719A1 (en) * 1993-05-27 1994-12-08 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
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DE19836110A1 (de) * 1998-08-10 2000-02-24 Biotul Bio Instr Gmbh Vorrichtung und Verfahren zur grenzflächennahen Mischung von Proben in Biosensorsystemen

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Also Published As

Publication number Publication date
DE10136008B4 (de) 2005-03-31
WO2003012389A2 (de) 2003-02-13
AU2002328867A1 (en) 2003-02-17
DE10136008A1 (de) 2003-02-20
EP1412533A2 (de) 2004-04-28

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