[go: up one dir, main page]

WO1999036528A3 - Transgener nicht-menschlicher säuger sowie verfahren zu seiner herstellung und seine verwendung - Google Patents

Transgener nicht-menschlicher säuger sowie verfahren zu seiner herstellung und seine verwendung Download PDF

Info

Publication number
WO1999036528A3
WO1999036528A3 PCT/DE1999/000116 DE9900116W WO9936528A3 WO 1999036528 A3 WO1999036528 A3 WO 1999036528A3 DE 9900116 W DE9900116 W DE 9900116W WO 9936528 A3 WO9936528 A3 WO 9936528A3
Authority
WO
WIPO (PCT)
Prior art keywords
human mammal
gene
transgenic non
utilization
homologous
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE1999/000116
Other languages
English (en)
French (fr)
Other versions
WO1999036528A2 (de
Inventor
Monica Hollstein
Qin Yang
Zhao-Qi Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Deutsches Krebsforschungszentrum DKFZ
Original Assignee
Deutsches Krebsforschungszentrum DKFZ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deutsches Krebsforschungszentrum DKFZ filed Critical Deutsches Krebsforschungszentrum DKFZ
Priority to JP2000540230A priority Critical patent/JP2002508960A/ja
Priority to EP99907250A priority patent/EP1049771A2/de
Publication of WO1999036528A2 publication Critical patent/WO1999036528A2/de
Publication of WO1999036528A3 publication Critical patent/WO1999036528A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4746—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used p53
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/15—Humanized animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0331—Animal model for proliferative diseases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Biophysics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Molecular Biology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur Erzeugung eines transgenen nicht-menschlichen Säugers, wobei eine embryonale Stammzellinie eines nicht-menschlichen Säugers mit einem geeigneten Rekombinationsvektor transfiziert wird, stabil transfizierte Zellclone isoliert werden und in geeignet vorbereitete weibliche Tiere eines nicht-menschlichen Säugers implantiert werden und deren Nachkommen auf das Vorhandensein des Genaustausches selektioniert werden, das dadurch gekennzeichnet ist, daß bei dem Rekombinationsereignis die Gesamtheit oder einen Teil des p53-Gens durch ein homologes p53-Gen oder einen homologen p53-Genabschnitt eines anderen Säugers funktionell ersetzt wird, wobei die jeweils eingeschleuste Sequenz als genomische Sequenz vorliegt. Außerdem betrifft die Erfindung den erhaltenen transgenen, nicht-menschlichen Säuger und seine Verwendung zum Austesten von Medikamenten, Chemikalien und Therapieansätzen.
PCT/DE1999/000116 1998-01-19 1999-01-19 Transgener nicht-menschlicher säuger sowie verfahren zu seiner herstellung und seine verwendung Ceased WO1999036528A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2000540230A JP2002508960A (ja) 1998-01-19 1999-01-19 トランスジェニック非ヒト哺乳動物、その作製方法およびその利用
EP99907250A EP1049771A2 (de) 1998-01-19 1999-01-19 Transgener nicht-menschlicher säuger sowie verfahren zu seiner herstellung und seine verwendung

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE1998101780 DE19801780A1 (de) 1998-01-19 1998-01-19 Transgener nicht-menschlicher Säuger sowie Verfahren zu seiner Herstellung und seine Verwendung
DE19801780.4 1998-01-19

Publications (2)

Publication Number Publication Date
WO1999036528A2 WO1999036528A2 (de) 1999-07-22
WO1999036528A3 true WO1999036528A3 (de) 1999-09-23

Family

ID=7855011

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1999/000116 Ceased WO1999036528A2 (de) 1998-01-19 1999-01-19 Transgener nicht-menschlicher säuger sowie verfahren zu seiner herstellung und seine verwendung

Country Status (4)

Country Link
EP (1) EP1049771A2 (de)
JP (1) JP2002508960A (de)
DE (1) DE19801780A1 (de)
WO (1) WO1999036528A2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017026383A1 (ja) * 2015-08-10 2017-02-16 国立大学法人三重大学 肺特異的にヒトｅｇｆｒを発現する非ヒト哺乳類のトランスジェニック動物

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992011874A1 (en) * 1991-01-04 1992-07-23 Baylor College Of Medicine Tumor susceptible non-human animals

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5569824A (en) * 1991-01-04 1996-10-29 Baylor College Of Medicine Transgenic mice containing a disrupted p53 gene

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992011874A1 (en) * 1991-01-04 1992-07-23 Baylor College Of Medicine Tumor susceptible non-human animals

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GONDO,Y ET AL.: "Gene replacement of the p53 gene with the lacZ gene in mouse gene in mouse embryonic stem cells and mice by using two steps of homologous brecombination", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS., vol. 94, no. 202, 1929 - 1907, ORLANDO, FL US, pages 94, XP002108196 *
LAVIGUEUR, A. ET AL.: "High incidence of lung, bone, and lymphoid tumors in transgenic mice overexpressing mutant alleles of the p53 oncogene", MOLECULAR AND CELLULAR BIOLOGY, vol. 9, no. 9, September 1989 (1989-09-01), WASHINGTON US, pages 3982 - 3991, XP002108195 *

Also Published As

Publication number Publication date
WO1999036528A2 (de) 1999-07-22
DE19801780A1 (de) 1999-07-22
EP1049771A2 (de) 2000-11-08
JP2002508960A (ja) 2002-03-26

Similar Documents

Publication Publication Date Title
CA2230759A1 (en) Chimeric animal and method for producing the same
TWI255853B (en) Method for modifying chromosomes
DE69841452D1 (de) Humanes Chromosomfragment und ein Vektor, der dies enthält
TWI315328B (en) Aromatic polymer, method for producing the same and uses thereof
IL126416A0 (en) Cultured inner cell mass cell lines derived from ungulate embryos
EP0842266A4 (de) Transgenes schwein und schweinezellen, die humane hla-gene aufweisen
CA2214018A1 (en) An immortalized mammal hybridoma fusion partner
EP1198169A4 (de) Verfahren der Zellreprogrammierung durch Herstellung eines Heterokaryons
WO2000036106A3 (en) Non-invasive evaluation of physiological response in a mammal
ATE281516T1 (de) Verfahren zur herstellung von säugetieren mit definierten genetischen eigenschaften
MXPA02002443A (es) Restauracion de telomeros y extension de la longevidad en animales clonados con celulas somaticas senescentes.
WO2000026357A3 (en) Transgenic and cloned mammals
TR200403501T2 (tr) Transgenik bitki ve metotları.
WO2001088192A3 (en) A method for generating hypermutable organisms
WO1999036528A3 (de) Transgener nicht-menschlicher säuger sowie verfahren zu seiner herstellung und seine verwendung
AU2002252984A1 (en) Stress resistance as a selectable marker for transgenic plants
WO2004003157A3 (en) Gene regulation in transgenic animals using a transposon-based vector
GB2369828A (en) Nuclear transfer with selected donor cells
WO2003102197A8 (en) Transgenic plants with controlled distribution of a trait to progeny
WO2002020752A3 (en) Cloned cells, embryos, and avians and methods, of producing them
WO2004053090A3 (en) Method and system for utilizing somatic cell nuclear transfer embryos as cell donors for additional nuclear transfer
DK1356031T3 (da) Effektiv kerneomprogrammering hos pattedyr
IL148781A0 (en) Methods of producing cloned and transgenic mammals
CA2311599A1 (en) Transgenic non-human mammals with an oncogenic mutant of raf gene 1
WO2003005810A3 (en) Cloned non-human mammals from contact inhibited donor cells

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): JP US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): JP US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 09600583

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 1999907250

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1999907250

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1999907250

Country of ref document: EP