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WO1999036528A3 - Mammifere transgenique non humain, procede permettant de l'obtenir et son utilisation - Google Patents

Mammifere transgenique non humain, procede permettant de l'obtenir et son utilisation Download PDF

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Publication number
WO1999036528A3
WO1999036528A3 PCT/DE1999/000116 DE9900116W WO9936528A3 WO 1999036528 A3 WO1999036528 A3 WO 1999036528A3 DE 9900116 W DE9900116 W DE 9900116W WO 9936528 A3 WO9936528 A3 WO 9936528A3
Authority
WO
WIPO (PCT)
Prior art keywords
human mammal
gene
transgenic non
utilization
homologous
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE1999/000116
Other languages
German (de)
English (en)
Other versions
WO1999036528A2 (fr
Inventor
Monica Hollstein
Qin Yang
Zhao-Qi Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Deutsches Krebsforschungszentrum DKFZ
Original Assignee
Deutsches Krebsforschungszentrum DKFZ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deutsches Krebsforschungszentrum DKFZ filed Critical Deutsches Krebsforschungszentrum DKFZ
Priority to JP2000540230A priority Critical patent/JP2002508960A/ja
Priority to EP99907250A priority patent/EP1049771A2/fr
Publication of WO1999036528A2 publication Critical patent/WO1999036528A2/fr
Publication of WO1999036528A3 publication Critical patent/WO1999036528A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4746—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used p53
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/15—Humanized animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0331—Animal model for proliferative diseases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Biophysics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Molecular Biology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un procédé permettant d'obtenir un mammifère transgénique non humain, selon lequel une cellule souche embryonnaire de mammifère non humain est transfectée avec un vecteur de recombinaison approprié, des clones cellulaires transfectés de manière stable sont isolés et implantés dans des femelles préparées de manière appropriée, d'un mammifère non humain, et leur progéniture est sélectionnée selon le critère de la présence de l'échange génique. Ce procédé se caractérise en ce que lors de la recombinaison, le gène p53 est remplacé de manière fonctionnelle, en totalité ou en partie, par un gène p53 homologue ou une section du gène p53 homologue d'un autre mammifère, la séquence insérée figure sous forme de séquence génomique. L'invention concerne en outre le mammifère transgénique non humain obtenu et son utilisation pour tester des médicaments, des produits chimiques et des préparations thérapeutiques.
PCT/DE1999/000116 1998-01-19 1999-01-19 Mammifere transgenique non humain, procede permettant de l'obtenir et son utilisation Ceased WO1999036528A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2000540230A JP2002508960A (ja) 1998-01-19 1999-01-19 トランスジェニック非ヒト哺乳動物、その作製方法およびその利用
EP99907250A EP1049771A2 (fr) 1998-01-19 1999-01-19 Mammifere transgenique non humain, procede permettant de l'obtenir et son utilisation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE1998101780 DE19801780A1 (de) 1998-01-19 1998-01-19 Transgener nicht-menschlicher Säuger sowie Verfahren zu seiner Herstellung und seine Verwendung
DE19801780.4 1998-01-19

Publications (2)

Publication Number Publication Date
WO1999036528A2 WO1999036528A2 (fr) 1999-07-22
WO1999036528A3 true WO1999036528A3 (fr) 1999-09-23

Family

ID=7855011

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1999/000116 Ceased WO1999036528A2 (fr) 1998-01-19 1999-01-19 Mammifere transgenique non humain, procede permettant de l'obtenir et son utilisation

Country Status (4)

Country Link
EP (1) EP1049771A2 (fr)
JP (1) JP2002508960A (fr)
DE (1) DE19801780A1 (fr)
WO (1) WO1999036528A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017026383A1 (fr) * 2015-08-10 2017-02-16 国立大学法人三重大学 Animal mammifère transgénique non-humain exprimant un egfr humain spécifiquement dans les poumons

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992011874A1 (fr) * 1991-01-04 1992-07-23 Baylor College Of Medicine Animaux non humains susceptibles d'avoir une tumeur

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5569824A (en) * 1991-01-04 1996-10-29 Baylor College Of Medicine Transgenic mice containing a disrupted p53 gene

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992011874A1 (fr) * 1991-01-04 1992-07-23 Baylor College Of Medicine Animaux non humains susceptibles d'avoir une tumeur

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GONDO,Y ET AL.: "Gene replacement of the p53 gene with the lacZ gene in mouse gene in mouse embryonic stem cells and mice by using two steps of homologous brecombination", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS., vol. 94, no. 202, 1929 - 1907, ORLANDO, FL US, pages 94, XP002108196 *
LAVIGUEUR, A. ET AL.: "High incidence of lung, bone, and lymphoid tumors in transgenic mice overexpressing mutant alleles of the p53 oncogene", MOLECULAR AND CELLULAR BIOLOGY, vol. 9, no. 9, September 1989 (1989-09-01), WASHINGTON US, pages 3982 - 3991, XP002108195 *

Also Published As

Publication number Publication date
WO1999036528A2 (fr) 1999-07-22
DE19801780A1 (de) 1999-07-22
EP1049771A2 (fr) 2000-11-08
JP2002508960A (ja) 2002-03-26

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