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WO1993019619A1 - Procede pour la destruction de microorganismes et d'enzymes: procede mts (mano-thermo-sonication) - Google Patents

Procede pour la destruction de microorganismes et d'enzymes: procede mts (mano-thermo-sonication) Download PDF

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Publication number
WO1993019619A1
WO1993019619A1 PCT/ES1993/000021 ES9300021W WO9319619A1 WO 1993019619 A1 WO1993019619 A1 WO 1993019619A1 ES 9300021 W ES9300021 W ES 9300021W WO 9319619 A1 WO9319619 A1 WO 9319619A1
Authority
WO
WIPO (PCT)
Prior art keywords
sonication
microorganisms
enzymes
thermo
destruction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/ES1993/000021
Other languages
English (en)
Spanish (es)
Inventor
Justino Burgos Gonzalez
Santiago Condon Uson
Pascual Lopez Buesa
Juan Antonio ORDOÑEZ PEREDA
Javier Raso Pueyo
Francisco Sala Trepat
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Zaragoza
Original Assignee
Universidad de Zaragoza
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad de Zaragoza filed Critical Universidad de Zaragoza
Publication of WO1993019619A1 publication Critical patent/WO1993019619A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
    • A61L2/025—Ultrasonics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B11/00—Preservation of milk or dairy products
    • A23B11/10—Preservation of milk or milk preparations
    • A23B11/16—Preservation of milk or milk preparations by irradiation, e.g. by microwaves
    • A23B11/162—Preservation of milk or milk preparations by irradiation, e.g. by microwaves by sonic or ultrasonic waves
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B2/00—Preservation of foods or foodstuffs, in general
    • A23B2/10—Preservation of foods or foodstuffs, in general by treatment with pressure variation, shock, acceleration or shear stress
    • A23B2/103—Preservation of foods or foodstuffs, in general by treatment with pressure variation, shock, acceleration or shear stress using sub- or super-atmospheric pressures, or pressure variations transmitted by a liquid or gas
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B2/00—Preservation of foods or foodstuffs, in general
    • A23B2/40—Preservation of foods or foodstuffs, in general by heating loose unpacked materials
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B2/00—Preservation of foods or foodstuffs, in general
    • A23B2/50—Preservation of foods or foodstuffs, in general by irradiation without heating
    • A23B2/57—Preservation of foods or foodstuffs, in general by irradiation without heating by treatment with ultrasonic waves

Definitions

  • MTS Process Hand-Thermo-Sonication
  • the methods used for the preservation of food and other products are especially directed to prevent or hinder the action of various agents of alteration, being among them the main responsible for most of the cases of alteration, enzymes and microorganisms.
  • Sanitation treatments are aimed at the destruction of microorganisms, pathogens for humans, transported by food or other biological fluids.
  • the heat treatment is one of the most important methods for the preservation of food and other alterable products in the medium and long term, since, in addition to prolonging the life of the same, it is the only one capable of guaranteeing, 0 for its destructive effect , their health. Therefore, heat treatments are frequently used, as a previous step to their subsequent dehydration or freezing.
  • thermo-ultrasonic effect As with the rest of the methods of food preservation is different for each microorganism and enzyme and for the different microenvironmental conditions (pH, aw etc.), by this method it has been achieved, by
  • the procedure of this patent consists, in essence, of driving the product under pressure through a receptacle in which it is maintained at the treatment temperature and subjected to an ultrasonic field.
  • Fig 1 shows an instrument in which the effect of the method
  • the instrument is constituted by:
  • a main vessel (A) of 600 ml. capacity which serves as a stable heating element of the assembly, equipped with a heating element (resistance) (1), a temperature sensor (Pt 100) (2) and a stirring shaft (3).
  • This element also houses the inoculum injection connections, which is carried out by means of a syringe operated by a solenoid (6) and sampling (7) which is carried out by means of an electrically activated valve, connected to a timer.
  • the set of electrical and electronic elements of the installation is regulated by a main module.
  • the installation also has other accessory elements such as: a fiber optic detector for the detection of sampling tubes (9) and a fraction collector. (10)
  • Fig 2 shows the survival lines (in which the logarithm of the number of survivors is represented against the corresponding treatment times) of a population of Bacillus subtilis subjected to ultrasound (- ⁇ ), to an isothermal heating at 105 S C () already an MTS process to 105 S C with an ultrasonic frequency of 20 Hz, an amplitude of 117 microns and a pressure of 300 KPa. () •
  • Fig. 3 shows the thermodestruction lines (constructed representing the logarithms of the decimal reduction times versus the corresponding treatment temperatures) of a population of Bacillus subtilis subjected to different thermal treatments and the corresponding MTS treatments at different temperatures with a frequency of 20 kHz, an amplitude of 117 microns and a pressure of 300kPa.
  • Fig 4 shows the relationship between Hand-Thermosonication pressure and decimal reduction times at 105 9 C of Bacillus subtilis.
  • Fig 5 shows the relationship between ultrasonic amplitude during different MTS processes (at 20 kHz frequency and 102 e C) and the decimal reduction times of Bacillus subtilis.
  • the spore suspensions were obtained by dragging, with sterile distilled water, a superficial culture of B. subtilis in nutrient agar with 1 ppm of manganese after an incubation period of 5 days at 42 S C.
  • subtilis subjected to manothermosonication are destroyed following a first-order reaction kinetics (at same as those subjected to a heat treatment) and in our conditions about 10 times faster (fig 2).
  • the simultaneous application of pressure, together with that of heat and ultrasound, allows the effect to be maintained at temperatures above the boiling point of water (100 -C) (fig 3), which makes it possible to use them at high temperatures.
  • the effectiveness of the MTS process is determined, among other factors by temperature, treatment time, pressure and sonication amplitude (figs 4 and 5).
  • the kinetics of the effect follow a well-defined course, which allows the corresponding calculations to be carried out and the MTS processing parameters most precisely defined for each particular objective to be defined.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Procédé pour la destruction de microorganismes et enzymes par application combinée de chaleur et d'ultrasons sous-pression (Procédé MTS) dans des conditions contrôlées de pression, temps, température, fréquence et amplitude de sonication. L'application d'ultrasons sous-pression combinée au traitement thermique génère par synergie l'effet destructeur sur les microorganismes et les enzymes, permettant ainsi de réduire le temps et/ou la température de traitement. Le rythme de destruction des micro-organismes et des enzymes soumis à la Mano-Thermo-Sonication suit une cinétique de réaction de premier ordre, ce qui permet de calculer les traitements de stérilisation ou d'hygiène à appliquer pour obtenir un effet prédéterminé. Une fois établies les conditions préalablement calculées de vitesse du flux, température, pression et intensité de sonication dans la chambre de Mano-Thermo-Sonication, le produit à traiter est pulsé à travers ladite chambre.
PCT/ES1993/000021 1992-03-31 1993-03-31 Procede pour la destruction de microorganismes et d'enzymes: procede mts (mano-thermo-sonication) Ceased WO1993019619A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ESP9200686 1992-03-31
ES09200686A ES2046944B1 (es) 1992-03-31 1992-03-31 Procedimiento para la destruccion de microorganismos y enzimas mediante la aplicacion combinada de calor y ultrasonidos bajo presion: proceso mts (mano-termo-sonicacion).

Publications (1)

Publication Number Publication Date
WO1993019619A1 true WO1993019619A1 (fr) 1993-10-14

Family

ID=8276574

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES1993/000021 Ceased WO1993019619A1 (fr) 1992-03-31 1993-03-31 Procede pour la destruction de microorganismes et d'enzymes: procede mts (mano-thermo-sonication)

Country Status (3)

Country Link
EP (1) EP0587854A1 (fr)
ES (1) ES2046944B1 (fr)
WO (1) WO1993019619A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2516665C1 (ru) * 2012-12-03 2014-05-20 Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Челябинская государственная агроинженерная академия" Устройство для очистки и обеззараживания молочной продукции
EP3402342A4 (fr) * 2016-01-15 2019-07-31 The Coca-Cola Company Traitement à haute pression continu de produits alimentaires et de boissons

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2291051B1 (es) * 2001-12-14 2009-01-16 Universidad Zaragoza Mejora de la textura del yogur mediante la manotermosonicacion (mts) previa de la leche.
ES2231030B1 (es) * 2003-10-29 2006-07-16 Universidad Politecnica De Cartagena. Termorresistometro para la medida de la resistencia al calor de microorganismos en condiciones controladas de temperatura, capaz de simular condiciones de tratamiento isotermico y no isotermo.
ES2366066B1 (es) * 2009-11-26 2012-05-16 Carlos V�?Zquez Montufo Equipo de filtración previsto para el filtrado de fluidos utilizados o contaminados y procedimiento de filtrado para fluidos utilizados o contaminados.
ES2526824B1 (es) 2012-08-09 2016-02-12 Universidad Miguel Hernández De Elche Equipo de expansión instantánea a vacío y ultrasonidos
FR3114942B1 (fr) * 2020-10-12 2024-03-22 Algama Procédé d'obtention d'une boisson de substitution au lait à base de microalgue par manothermosonication

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4211744A (en) * 1978-05-24 1980-07-08 Biophysics Research & Consulting Corporation Process for ultrasonic pasteurization
US5026564A (en) * 1990-07-02 1991-06-25 Hayden Steven M Apparatus and method for treatment of various liquid or slurry by ultrasonification in conjuction with heat and pressure

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4211744A (en) * 1978-05-24 1980-07-08 Biophysics Research & Consulting Corporation Process for ultrasonic pasteurization
US5026564A (en) * 1990-07-02 1991-06-25 Hayden Steven M Apparatus and method for treatment of various liquid or slurry by ultrasonification in conjuction with heat and pressure

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2516665C1 (ru) * 2012-12-03 2014-05-20 Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Челябинская государственная агроинженерная академия" Устройство для очистки и обеззараживания молочной продукции
EP3402342A4 (fr) * 2016-01-15 2019-07-31 The Coca-Cola Company Traitement à haute pression continu de produits alimentaires et de boissons

Also Published As

Publication number Publication date
ES2046944B1 (es) 1994-08-16
EP0587854A1 (fr) 1994-03-23
ES2046944A1 (es) 1994-02-01

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