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WO1981001290A1 - Gel de polyacrylamide pour application medicale et biologique et son procede de preparation - Google Patents

Gel de polyacrylamide pour application medicale et biologique et son procede de preparation Download PDF

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Publication number
WO1981001290A1
WO1981001290A1 PCT/SU1980/000104 SU8000104W WO8101290A1 WO 1981001290 A1 WO1981001290 A1 WO 1981001290A1 SU 8000104 W SU8000104 W SU 8000104W WO 8101290 A1 WO8101290 A1 WO 8101290A1
Authority
WO
WIPO (PCT)
Prior art keywords
gel
aκρilamida
purposes
medical
polylamide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/SU1980/000104
Other languages
English (en)
Russian (ru)
Inventor
V Gashinsky
A Sokolyuk
I Bilko
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KIEVSKY MEDITSINSKY INST
KI MED I
Original Assignee
KIEVSKY MEDITSINSKY INST
KI MED I
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from SU792831351A external-priority patent/SU977466A1/ru
Application filed by KIEVSKY MEDITSINSKY INST, KI MED I filed Critical KIEVSKY MEDITSINSKY INST
Priority to JP80501574A priority Critical patent/JPS6240997B2/ja
Priority to DE803050012T priority patent/DE3050012A1/de
Publication of WO1981001290A1 publication Critical patent/WO1981001290A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/74—Synthetic polymeric materials
    • A61K31/765—Polymers containing oxygen
    • A61K31/78—Polymers containing oxygen of acrylic acid or derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/16—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/52—Amides or imides
    • C08F220/54—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide
    • C08F220/56—Acrylamide; Methacrylamide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B1/00—Optical elements characterised by the material of which they are made; Optical coatings for optical elements
    • G02B1/04—Optical elements characterised by the material of which they are made; Optical coatings for optical elements made of organic materials, e.g. plastics
    • G02B1/041—Lenses
    • G02B1/043—Contact lenses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers

Definitions

  • the invention is available for polylactylamide gel 5 for medical and biological purposes and the method of its use.
  • the invention is intended for use in medicine and biology.
  • the preceding level is the present in the case of microprocessor.
  • 20% of the product is 3 to 20 parts by weight of water per I part of the consumable mixture of monomers, which, in turn, consumes 0.01% of the total consumption or the consumption of alcohol.
  • the indicated polylamide gel is the basis
  • the one indicated synthetic environment also contains a selectable quantity of toxic sources.
  • ⁇ me ⁇ g ⁇ , ⁇ a ⁇ ⁇ a ⁇ sin ⁇ e ⁇ iches ⁇ aya ⁇ sn ⁇ va imee ⁇ ⁇ isl ⁇ ⁇ e " ⁇ ea ⁇ tsiyu / ⁇ 3,5 - 4 / are not any ' ⁇ i ⁇ a ⁇ elnye subs ⁇ a ⁇ y m ⁇ gu ⁇ by ⁇ zvedy in ⁇ sn ⁇ vu E ⁇ ⁇ bs ⁇ ya ⁇ el- 35 s ⁇ v ⁇ ⁇ g ⁇ anichivae ⁇ v ⁇ zm ⁇ n ⁇ s ⁇ is ⁇ lz ⁇ vaniya u ⁇ azann ⁇ y sin ⁇ e ⁇ iches ⁇ y ⁇ sn ⁇ vy.. . - 2 -
  • the aforementioned method includes the elimination of aka rilamide and methylene bis acrylamide in a separate environment from the following with its natural source.
  • the washing of the product is beneficial, it also removes toxic substances from the body and does not neglect the product
  • the size of a polylamide gel, 20 contacting with living organisms, is not stable.
  • P ⁇ s ⁇ avlennaya task ⁇ eshae ⁇ sya ⁇ em, ch ⁇ izves ⁇ ny ⁇ lia ⁇ ilamidny gel, s ⁇ de ⁇ zhaschy ⁇ lime ⁇ a ⁇ ilamida and me ⁇ ilen bis a ⁇ ilamida, s ⁇ glasn ⁇ iz ⁇ b ⁇ e ⁇ eniyu, d ⁇ l- 30 ni ⁇ oln ⁇ s ⁇ de ⁇ zhi ⁇ ⁇ izi ⁇ l ⁇ giches ⁇ y ⁇ as ⁇ v ⁇ ⁇ i following present s ⁇ de ⁇ zhanii ⁇ m ⁇ nen ⁇ v / in wt. /: polylactylamide - 3.0 - 28.0 ⁇ _ * • biological treatment - 72.0 - 97.0
  • the indicated polyl-amylamide gel is not toxic and possesses high efficiency, hydro- philicity, elasticity, and therefore, stability, stability.
  • a polylactic gel is available in a variety of 5 with microorganisms, cartilage, tissue, and volatility, which makes it possible to stabilize it. All this makes it possible to have a harmless contact with polylamide gel with lives of organisms and 10 thereby significantly increase the risk of harm to health.
  • polylactylamide gel in ka-20 has a very large proportion of 0.5% water consumption, which is due to the following: 0.5% WATER CONSUMPTION 25 CHLOROUS INDUSTRY - 85.0 - 94.0
  • the polylactylamide gel in the physiological product contains a 0.9% aqueous distillation unit after the next medium /: 35 polylactylamide - 5.0 - 18.0
  • the indicated modification of polylamine amide gel ensures optimal accessibility to live organisms and is used in cases of neglect.
  • the indicated modi fi cation of the method will allow the use of polylamine amide, as a result of the historic art. When this is done, the automation of the tissues of the eye during implantation is reduced due to the performance of minimal discharges, and the complete complete care of the eye. ''
  • the indicated method of medication will help to use polylamine amide in the form of a contact lens. With this, a long-lasting continuous wearing is ensured when the anomalies of the anomalies are repaired in a wide range of cases.
  • Polyacrylamide gel is obtained by the elimination of acrylamide and methylene bis acrylamide or other alkalidine bis acrylamide.
  • the basic mixture typically contains 80-99.5% by weight acrylamide, 0.5-20%. methyl bis acrylamide or another suitable alkylidine bis acrylamide.
  • the original components are damaged by a physiological solution. 3 In the case of a physiological product, use: 0, 5% water. distilled water or 0, 9th water
  • the optimization of the original methods may result in no load or heat of the reactive mixture, addition of the known initiation, catalysis.
  • the speed of the reaction to the polymerization is directly related to the tempera- ture, the quantity of catalysis and the intensity of the irradiation.
  • Conventional catalytic converts in the amount of 0.05 - 0, mass% of the quantity of initial sources.
  • the reactive mixture may also be prepared and mixed by drying the other products with the following processing in a physiological process. To speed up the recovery, the physiological growth is heated. It is possible to realize a reduction in the volume of a reactive mixture in the volume of a given concentration - in glass, metal, and ceramic; in fact, The gel obtained in the process of polymerization turns on the size and size of the used capacitance.
  • the washing of the gel is not based on the usual methods, which are not subject to the reaction of optimization, may be subject to the usual conditions and in case of an elevated temperature. And - 7 - by this means, the original measures that did not enter into the reaction, are resolved in a physiological process and are transferred from the gel to the negative. By changing the physiological solution, it is possible to completely remove toxic products from the gel.
  • Saturation of the gel with substances for the nutrition of microorganisms and the culture of the cell can be carried out before or after sterilization.
  • the saturation method is shared by a non-nutritious substance.
  • thermostable substances in the foodstuff Subject to the availability of thermostable substances in the foodstuff, saturations are carried out after sterilization.
  • the composition of the foodstuffs is distributed by foodstuffs of large groups or species of microorganisms and cages. To saturate
  • livestock and human cells are used in well-known research methods.
  • Polyacrylamide gel in accordance with the invention containing mass% polylactylamide - II, 0.5 ⁇ -different water treatment - 89, were obtained
  • the resulting disks were placed in a container and poured with a 0.5% aqueous solution from a solution of 20 ml of solution in the first disk. The disks were then kept in a 0.5-liter one-way cooling unit for 12 hours, replacing the disk after every 4 hours.
  • the obtained polyl-amylamide gel is not toxic; it possesses high efficiency, hydro- philicity, cosmetic, surgical, thermal stability.
  • the gel was saturated with food substrates
  • Polyacrylamide gel in accordance with the invention containing / in mass ⁇ / poliakrilamide - 8.0, physiological solution - 92.0, the case was received.
  • Opportunity 5 in Example I For the sake of physiological use, a 0.9-liter aqueous solution was used.
  • the obtained polyl-amylamide gel was not toxic, 'it was equipped with a quick accessibility, hydraulics, elasticity, processability, and stability.
  • Gel ⁇ sh ⁇ saturated subs ⁇ a ⁇ ami for power The mi ⁇ ga- nizm ⁇ v, ⁇ le ⁇ ' ⁇ zhiv ⁇ ny ⁇ and chel ⁇ ve ⁇ a.-
  • Fully-disgusting gel in accordance with the invention, containing / in wt.% / Polylactylamide - 3.0; used the extract of the Kingge-Loca.
  • Polyacrylamide gel in accordance with the invention containing / s of mass. / polylamide-11.0, a physiological product - 89.0, were obtained by method 5 described in Example I. Three methods of physical use were used.
  • the gel plates were put on a stand-alone circuit, and 10 sizes, sizes and life characteristics of fibers, kletos ⁇ 1, ⁇ and ⁇ were used for the specified case are given for 6.
  • Polyacrylamide gel in accordance with the invention containing / in mass. / polylamide - 20.0, physiological product - 80.0 were obtained from the apparatus described in Example I. 25, due to physical injury, it was used
  • polylamine amide gel The properties of the obtained polylamine amide gel were similar to the properties described in Example 2.4.
  • the size, size and life of the fibers, the plet ⁇ 5- ⁇ s ⁇ and ⁇ on the indicated gel were achieved up to 8-10 days at a temperature of 4 ° ⁇ .
  • Polyacrylamide gel in accordance with the invention containing poliakrilamide - 15.0, physiological solution -85.0, was obtained by the method described in I.
  • the properties of the obtained polylamine amide gel were similar to those described in Example 2.4.
  • the small cell that grew on the gel plates was easily washed off, so that it could easily accumulate the cell mass.
  • the Needle was used as a physiological disaster.
  • the initial preparations were made for basic products / ⁇ , 3, C /.
  • Preparation of 25 discharges ⁇ - 5 ml of methyl ester of dihydrogen was dissolved in 995 ml of a 0, 9% aqueous solution of the chemical treatment.
  • Food preparation ⁇ - 7.35 g of methylene-bis-acryl amide was dissolved in 350 ml of a 0, 9% aqueous solution, which was processed
  • the ⁇ , ⁇ , and C discharges were taken in large volumes 1: 2: 4. - 15 -
  • the volume of the basic equipment in connection with the production of the working mixture may be altered due to the unavoidable degree of elasticity.
  • 0.5 ml of the prepared reactive mixture was poured into the reaction, the internal area of the quick-coupled model wastes a separate patient.
  • the time for the initialization of the reaction mixture is ' 3 min. ⁇ and temperature of 20 ° ⁇ . At the end of the indicated time period, the resulting artifact has been removed from the previously indicated process. Artificial.
  • the size of the heater is characterized by the following parameters: The radius of the front of the rear is 27.22 mm, the rear is small and the dimer is 18 mm.
  • the washout was washed in 20 ml of 0.9 - a new, clean product for the first two days with a quick change of solution.
  • the company was stabilized in a 0.9-year-old industrial unit for 40 minutes. he kept in the same storage room for use in a hermetic closed court.
  • Polyacrylamide gel in accordance with the invention containing / in wt.% / Polylacrylamide - 5.0, physiological solution - 95, received a trial,
  • Example 10 20 nd in Example 10.
  • the 0.9th aqueous solution was used. Polymization was carried out in the factory, while the internal area of the model was simulated by the device.
  • a soft contact lens was obtained with the following parameters: Radius of the front side of the front -
  • ⁇ ⁇ aches ⁇ ve ⁇ izi ⁇ l ⁇ giches ⁇ g ⁇ ⁇ as ⁇ - v ⁇ a is ⁇ lz ⁇ vali 0
  • the 9th Western Union is located in the southern part of the country.
  • Example 13 Comparative / Polylamide gel, containing / in wt.% / Polylacrylamide - 2.0, the physiological solution - 98.0, we have taken the a new disinfectant.
  • the obtained polylactylamide gel had a convenient structure that did not allow the receiving of the gel plate, - 18 - their washing and saturation of food for the supply of microorganisms, live and human cells, planting of microorganisms and carcasses.
  • Example 14 Comparative /
  • Polyacrylamide gel in accordance with the invention containing / in mass. / polylamide - 29.0, physiological product - 71.0 were obtained from the method described in Example I.
  • the 0.9-liter water treatment unit was used as a physiological product.
  • this received poliakrilamidny gel had a very tight interference and there was a lot of bending. Flushing the gel plate from the original components was not very effective, it took a long time / 15 or more /.
  • the gel was saturated with substrates for food of microorganisms and the culture of cells, quickly dried with the appearance of cracks at 37 ° C and was detected in the cups.
  • the polylactylamide gel can be used as a simple base for the application of healthy substances for the development of the disease.
  • polylamilide gel in the form of a clean and healthy environment ensures that there is a slight increased risk of increased physical activity.
  • P ⁇ lia ⁇ ilamidny gel ⁇ a ⁇ sin ⁇ e ⁇ iches ⁇ y ⁇ e ⁇ a ⁇ a ⁇ imee ⁇ izves ⁇ ny and ⁇ s ⁇ yanny s ⁇ s ⁇ av, ch ⁇ ⁇ bes ⁇ echivae ⁇ v ⁇ s ⁇ izv ⁇ 'dim ⁇ s ⁇ ⁇ l ⁇ n ⁇ y ⁇ sn ⁇ vy ⁇ i ⁇ a ⁇ elny ⁇ s ⁇ ed and related e ⁇ im s ⁇ anda ⁇ izatsiyu mi ⁇ bi ⁇ l ⁇ giches ⁇ i ⁇ used sled ⁇ vany, ⁇ zv ⁇ lyayuschuyu s ⁇ s ⁇ avlya ⁇ ⁇ ezul ⁇ a ⁇ y ⁇ az- lichny ⁇ lab ⁇ a ⁇ y.
  • the production of polylamide gel can be automated.
  • Sterilization may be carried out by the general methods and in ordinary conditions.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Polymers & Plastics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Cell Biology (AREA)
  • Dermatology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Medicinal Preparation (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Polyamides (AREA)

Abstract

Un gel de polyacrylamide pour application medicale et biologique contient du polyacrylamide et une solution physiologique dont les proportions en pourcentages ponderaux sont les suivantes: polyacrylamide: 3,0 a 28,0; solution physiologique: 72,0 a 97,0. Le procede de preparation de ce gel de polyacrylamide comprend la polymerisation d'acrylamide avec du methylene-bis-acrylamide, et l'elution du produit final, la polymerisation et l'elution s'effectuant dans le milieu de la solution physiologique. Le gel de polyacrylamide peut etre utilise comme base de milieux nutritifs pour la croissance de micro-organismes, de lentille cristalline artificielle et de lentille de contact elastique.
PCT/SU1980/000104 1979-11-06 1980-06-19 Gel de polyacrylamide pour application medicale et biologique et son procede de preparation Ceased WO1981001290A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP80501574A JPS6240997B2 (fr) 1979-11-06 1980-06-19
DE803050012T DE3050012A1 (de) 1979-11-06 1980-06-19 Polyacrylamide gel for medical and biological application and method of its preparation

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
SU2831351 1979-11-06
SU792831351A SU977466A1 (ru) 1979-11-06 1979-11-06 Полиакриламидный гель дл медико-биологических целей и способ его получени
SU2912552 1980-02-28
SU2912551 1980-02-28

Publications (1)

Publication Number Publication Date
WO1981001290A1 true WO1981001290A1 (fr) 1981-05-14

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PCT/SU1980/000104 Ceased WO1981001290A1 (fr) 1979-11-06 1980-06-19 Gel de polyacrylamide pour application medicale et biologique et son procede de preparation

Country Status (3)

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DE (1) DE3050012A1 (fr)
GB (1) GB2114578B (fr)
WO (1) WO1981001290A1 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4454065A (en) * 1982-05-18 1984-06-12 Smithkline Beckman Corporation Oligopeptide prodrugs
US4939150A (en) * 1987-01-30 1990-07-03 Gashinsky Vladimir V Method for preparing dense nutrient medium for culturing microorganisms
US5415864A (en) * 1990-04-18 1995-05-16 University Of Utah Research Foundation Colonic-targeted oral drug-dosage forms based on crosslinked hydrogels containing azobonds and exhibiting PH-dependent swelling
US7162291B1 (en) 2000-03-01 2007-01-09 Mirabel Medical Systems Ltd. Uniform, disposable, interface for multi-element probe
US7186419B2 (en) 2000-08-25 2007-03-06 Contura Sa Polyacrylamide hydrogel for arthritis
US7678146B2 (en) 2000-08-25 2010-03-16 Contura A/S Polyacrylamide hydrogel and its use as an endoprosthesis

Families Citing this family (17)

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US4540568A (en) * 1982-10-14 1985-09-10 Trager Seymour F Injectionable viscoelastic ophthalmic gel
AU4198785A (en) * 1984-05-07 1985-11-14 Lloyd A. Ferreira Conductive material and biomedical electrode
GB2162198B (en) * 1984-07-27 1987-12-02 Ki Med I Process for producing dense nutrient medium for culturing microorganisms and macroorganism cell cultures
DE3430316A1 (de) * 1984-08-17 1986-02-27 Ki Medicinskij I Im Akademika Verfahren zur herstellung eines fluessigen naehrbodens zur zuechtung von mikroorganismen und zellenkulturen von makroorganismen
EP0237267B1 (fr) * 1986-03-11 1991-01-23 Barry Anthony Thompson Manière de nourrir du bétail avec des polymères
US5480950A (en) * 1992-09-28 1996-01-02 Kabi Pharmacia Ophthalmics, Inc. High refractive index hydrogels and uses thereof
US5316704B1 (en) * 1992-09-28 1996-05-21 Kabi Pharmacia Ophthalmics Inc Process for fabricating full sized expansible hydrogel intraocular lenses
WO1994013717A1 (fr) * 1992-12-04 1994-06-23 958075 Ontario Inc. Carrying On Business As Eurocan Ventures Procede de production d'une lentille de contact douce
US5439950A (en) * 1994-06-27 1995-08-08 Kabi Pharmacia Ophthalmics, Inc. Water miscible non-hydrolyzable cross-linkers and high refractive index hydrogels prepared therewith
UA10911C2 (uk) * 1994-08-10 1996-12-25 Мале Впроваджувальне Підприємство "Іhтерфалл" Біосумісhий гідрогель
US5941909A (en) * 1995-02-14 1999-08-24 Mentor Corporation Filling material for soft tissue implant prostheses and implants made therewith
US6972194B1 (en) * 1999-12-08 2005-12-06 Dmitry Vladimirovich Zybin Use of polyacrylamide gel for forming a connective-tissue capsule in a mammal for cultivating allogenic and xenogenic cells
PT1287048E (pt) 2000-08-25 2005-08-31 Contura Sa Hidrogel de poliacrilamida e sua utilizacao como endoprotese
ES2298195T3 (es) 2001-09-28 2008-05-16 Biopharma Development Ltd Hidrogel biocompatible multifuncional y su proceso de produccion.
KR100501225B1 (ko) * 2001-12-13 2005-07-18 휴젤(주) 모노머 제거 폴리아크릴아마이드 및 그의 제조방법
RU2236872C1 (ru) 2002-04-10 2004-09-27 Общество с ограниченной ответственностью "Витагель" Полифункциональный биосовместимый гидрогель и способ его получения
CN103224679B (zh) * 2013-05-09 2015-10-21 重庆大学 壳聚糖聚丙烯酰胺水凝胶基底材料及其制备方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3046201A (en) * 1961-07-17 1962-07-24 American Cyanamid Co Synthetic culture media
GB1288438A (fr) * 1969-11-03 1972-09-06
GB1319751A (en) * 1970-03-26 1973-06-06 Ceskoslovenska Akademie Ved Method of manufacturing articles exposed to a repeated or longtermed contact with live tissues or mucous membranes
SU659619A1 (ru) * 1972-04-21 1979-04-30 Киевский Медицинский Институт Им. Академика А.А.Богомольца Способ приготовлени питательной среды дл культивировани микроорганизмов

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3046201A (en) * 1961-07-17 1962-07-24 American Cyanamid Co Synthetic culture media
GB1288438A (fr) * 1969-11-03 1972-09-06
GB1319751A (en) * 1970-03-26 1973-06-06 Ceskoslovenska Akademie Ved Method of manufacturing articles exposed to a repeated or longtermed contact with live tissues or mucous membranes
SU659619A1 (ru) * 1972-04-21 1979-04-30 Киевский Медицинский Институт Им. Академика А.А.Богомольца Способ приготовлени питательной среды дл культивировани микроорганизмов

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4454065A (en) * 1982-05-18 1984-06-12 Smithkline Beckman Corporation Oligopeptide prodrugs
US4939150A (en) * 1987-01-30 1990-07-03 Gashinsky Vladimir V Method for preparing dense nutrient medium for culturing microorganisms
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GB2114578A (en) 1983-08-24

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