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UY37312A - Sustituciones nucleotídicas predeterminadas - Google Patents

Sustituciones nucleotídicas predeterminadas

Info

Publication number
UY37312A
UY37312A UY0001037312A UY37312A UY37312A UY 37312 A UY37312 A UY 37312A UY 0001037312 A UY0001037312 A UY 0001037312A UY 37312 A UY37312 A UY 37312A UY 37312 A UY37312 A UY 37312A
Authority
UY
Uruguay
Prior art keywords
dpcr
finding
substitutions
nucleotide
mutated
Prior art date
Application number
UY0001037312A
Other languages
English (en)
Inventor
Toni Wendt
Ole Olsen
Knudsen Søren
Cecilie Thomsen Hanne
Alexander Striebeck
Birgitte Skadhauge
Wohlfahrt Rasmussen Magnus
Massimiliano Carciofi
Original Assignee
Carlsberg As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carlsberg As filed Critical Carlsberg As
Publication of UY37312A publication Critical patent/UY37312A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4624—Hordeum vulgarus [barley]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Physiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

En los enfoques de mejoramiento de plantas tradicionales, puede utilizarse mutagénesis química para introducir sustituciones nucleotídicas aleatoriamente en el genoma de una planta, es decir sin posibilidades de controlar los sitios de los cambios nucleotídicos. A causa de las complejidades genómicas, la probabilidad estadística es extremadamente baja en cuanto a hallar una sustitución nucleotídica predeterminada. La presente invención, sin embargo, demuestra cómo se desarrolla un uso novedoso, alternativo, de la reacción en cadena de polimerasa digital (dPCR), preferiblemente dPCR en gotas (ddPCR), para explotar el hallazgo de sustituciones nucleotídicas específicas en genes mutados. La plataforma completa comprende un método de relevamiento con una biblioteca de organismos mutagenizados, sistemas basados en PCR digital y una configuración para propagar y analizar organismos mutados identificados.
UY0001037312A 2016-07-01 2017-06-30 Sustituciones nucleotídicas predeterminadas UY37312A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DKPA201670485 2016-07-01

Publications (1)

Publication Number Publication Date
UY37312A true UY37312A (es) 2018-02-28

Family

ID=56681913

Family Applications (1)

Application Number Title Priority Date Filing Date
UY0001037312A UY37312A (es) 2016-07-01 2017-06-30 Sustituciones nucleotídicas predeterminadas

Country Status (25)

Country Link
US (2) US11884972B2 (es)
EP (3) EP3693458A1 (es)
JP (2) JP7208797B2 (es)
CN (2) CN114959098A (es)
AR (1) AR110103A1 (es)
AU (3) AU2017288960B2 (es)
BR (1) BR112018076429A2 (es)
CA (1) CA3026016A1 (es)
CL (1) CL2018003710A1 (es)
CO (1) CO2019000828A2 (es)
CR (1) CR20190040A (es)
CU (1) CU20180158A7 (es)
DK (1) DK3478832T3 (es)
EA (1) EA201892809A1 (es)
ES (2) ES3032697T3 (es)
IL (1) IL263451B (es)
MA (3) MA52594A (es)
MX (2) MX2018016110A (es)
PE (1) PE20190228A1 (es)
PL (2) PL3478832T3 (es)
PT (1) PT3478832T (es)
SG (1) SG11201811021YA (es)
UY (1) UY37312A (es)
WO (1) WO2018001884A1 (es)
ZA (3) ZA201808218B (es)

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EP3693458A1 (en) * 2016-07-01 2020-08-12 Carlsberg A/S Method of identifying a sub-pool comprising a mutant organism
CA3085987A1 (en) 2017-12-28 2019-07-04 Carlsberg A/S Cereal plants with improved cell wall properties
WO2020012004A1 (en) * 2018-07-13 2020-01-16 Hifibio Sas Use of droplet single cell epigenome profiling for patient stratification
WO2020011998A1 (en) * 2018-07-13 2020-01-16 Hifibio Sas Use of droplet single cell epigenome profiling for patient stratification
CN108913698B (zh) * 2018-07-25 2021-10-26 安徽农业大学 一种与小麦穗发芽抗性/感性相关的caps标记及其应用
CN110724741B (zh) * 2019-07-24 2020-05-19 艾普拜生物科技(苏州)有限公司 一种检测微小残留白血病相关融合基因的引物、探针及其试剂盒
WO2021034191A1 (en) 2019-08-16 2021-02-25 Heineken Supply Chain B.V. Production of an alcohol-free beverage
EP3785529A1 (en) 2019-08-28 2021-03-03 Carlsberg A/S Barley plants with altered protein content in grains
CA3152086A1 (en) * 2019-10-10 2021-04-15 Carlsberg A/S Methods for preparing mutant plants
US20240043911A1 (en) * 2020-02-06 2024-02-08 The Administrators Of The Tulane Educational Fund Crispr-based assay for detecting tb in bodily fluids
BR112022015008A2 (pt) 2020-03-02 2022-10-11 Carlsberg As Plantas de cevada com alto limite de atividade de dextrinase
EP4029935A1 (en) 2021-01-14 2022-07-20 Carlsberg A/S S. eubayanus strain and hybrids thereof
AU2022375767A1 (en) * 2021-10-25 2024-05-02 Gt Molecular, Inc. Off-target blocking sequences to improve target discrimination by polymerase chain reaction
CN118510388A (zh) 2021-12-22 2024-08-16 巴斯夫农业种子解决方案美国有限责任公司 改进的用于基因组编辑事件的筛选方法
WO2023170272A1 (en) 2022-03-11 2023-09-14 Carlsberg A/S Modulation of protein levels
EP4618742A1 (en) 2022-11-16 2025-09-24 University of Copenhagen Plants with low seed glucosinolate content
WO2024133900A1 (en) 2022-12-22 2024-06-27 University Of Copenhagen Improved (-)-sparteine production
AU2024265403A1 (en) 2023-05-01 2025-10-16 Carlsberg A/S Barley with improved properties
WO2025108936A1 (en) * 2023-11-20 2025-05-30 Eth Zurich Method for providing genetically-modified cells
CN117965576B (zh) * 2024-02-01 2024-08-27 北京林业大学 一种王百合苷生物合成基因LlHCT1及其应用
EP4644572A1 (en) * 2024-04-29 2025-11-05 KWS SAAT SE & Co. KGaA Method for analysing trait purity in large seed bulks

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Also Published As

Publication number Publication date
CN109477087B (zh) 2022-06-24
NZ749382A (en) 2025-06-27
SG11201811021YA (en) 2019-01-30
PL3478832T3 (pl) 2022-06-06
IL263451B (en) 2022-08-01
AU2019268102B2 (en) 2020-03-05
EP3693458A1 (en) 2020-08-12
ES2916221T3 (es) 2022-06-29
CO2019000828A2 (es) 2019-04-30
AU2017288960B2 (en) 2019-12-12
US20240182961A1 (en) 2024-06-06
JP2022101703A (ja) 2022-07-06
DK3478832T3 (da) 2022-05-30
CN114959098A (zh) 2022-08-30
ZA201907775B (en) 2022-09-28
ES3032697T3 (en) 2025-07-23
EP3478832A1 (en) 2019-05-08
US12428678B2 (en) 2025-09-30
IL263451A (en) 2019-01-31
CL2018003710A1 (es) 2019-03-22
JP2019524083A (ja) 2019-09-05
AU2019268102A1 (en) 2019-12-12
US11884972B2 (en) 2024-01-30
MA45531A (fr) 2019-05-08
ZA201808218B (en) 2020-02-26
ZA201907774B (en) 2022-07-27
EP3792357A1 (en) 2021-03-17
AU2017288960A1 (en) 2019-01-17
MA52594A (fr) 2021-03-17
CR20190040A (es) 2019-05-09
US20190194723A1 (en) 2019-06-27
EP3478832B1 (en) 2022-02-23
EA201892809A1 (ru) 2019-06-28
CU20180158A7 (es) 2019-08-06
EP3792357C0 (en) 2025-05-07
PE20190228A1 (es) 2019-02-13
WO2018001884A1 (en) 2018-01-04
AR110103A1 (es) 2019-02-27
PT3478832T (pt) 2022-05-19
MX2018016110A (es) 2019-05-30
PL3792357T3 (pl) 2025-07-07
AU2019268103A1 (en) 2019-12-12
MA54568A (fr) 2021-10-27
EP3792357B1 (en) 2025-05-07
CA3026016A1 (en) 2018-01-04
CN109477087A (zh) 2019-03-15
MX2022014965A (es) 2023-01-11
JP7539428B2 (ja) 2024-08-23
BR112018076429A2 (pt) 2019-10-01
AU2019268103B2 (en) 2020-03-12
JP7208797B2 (ja) 2023-01-19

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