SG11202102029TA - Methods for generating spatially barcoded arrays - Google Patents
Methods for generating spatially barcoded arraysInfo
- Publication number
- SG11202102029TA SG11202102029TA SG11202102029TA SG11202102029TA SG11202102029TA SG 11202102029T A SG11202102029T A SG 11202102029TA SG 11202102029T A SG11202102029T A SG 11202102029TA SG 11202102029T A SG11202102029T A SG 11202102029TA SG 11202102029T A SG11202102029T A SG 11202102029TA
- Authority
- SG
- Singapore
- Prior art keywords
- methods
- generating spatially
- spatially barcoded
- barcoded arrays
- arrays
- Prior art date
Links
- 238000003491 array Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Lubricants (AREA)
- Compositions Of Macromolecular Compounds (AREA)
Applications Claiming Priority (43)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862723964P | 2018-08-28 | 2018-08-28 | |
| US201862723950P | 2018-08-28 | 2018-08-28 | |
| US201862723957P | 2018-08-28 | 2018-08-28 | |
| US201862723972P | 2018-08-28 | 2018-08-28 | |
| US201862723960P | 2018-08-28 | 2018-08-28 | |
| US201862723970P | 2018-08-28 | 2018-08-28 | |
| US201862724483P | 2018-08-29 | 2018-08-29 | |
| US201862724561P | 2018-08-29 | 2018-08-29 | |
| US201862724487P | 2018-08-29 | 2018-08-29 | |
| US201862724489P | 2018-08-29 | 2018-08-29 | |
| US201862779342P | 2018-12-13 | 2018-12-13 | |
| US201962788885P | 2019-01-06 | 2019-01-06 | |
| US201962788905P | 2019-01-06 | 2019-01-06 | |
| US201962788867P | 2019-01-06 | 2019-01-06 | |
| US201962788871P | 2019-01-06 | 2019-01-06 | |
| US201962788897P | 2019-01-06 | 2019-01-06 | |
| US201962812219P | 2019-02-28 | 2019-02-28 | |
| US201962819467P | 2019-03-15 | 2019-03-15 | |
| US201962819449P | 2019-03-15 | 2019-03-15 | |
| US201962819468P | 2019-03-15 | 2019-03-15 | |
| US201962819448P | 2019-03-15 | 2019-03-15 | |
| US201962819458P | 2019-03-15 | 2019-03-15 | |
| US201962819456P | 2019-03-15 | 2019-03-15 | |
| US201962819496P | 2019-03-15 | 2019-03-15 | |
| US201962819486P | 2019-03-15 | 2019-03-15 | |
| US201962819478P | 2019-03-15 | 2019-03-15 | |
| US201962822627P | 2019-03-22 | 2019-03-22 | |
| US201962822575P | 2019-03-22 | 2019-03-22 | |
| US201962822565P | 2019-03-22 | 2019-03-22 | |
| US201962822592P | 2019-03-22 | 2019-03-22 | |
| US201962822605P | 2019-03-22 | 2019-03-22 | |
| US201962822554P | 2019-03-22 | 2019-03-22 | |
| US201962822649P | 2019-03-22 | 2019-03-22 | |
| US201962822618P | 2019-03-22 | 2019-03-22 | |
| US201962822632P | 2019-03-22 | 2019-03-22 | |
| US201962839320P | 2019-04-26 | 2019-04-26 | |
| US201962839223P | 2019-04-26 | 2019-04-26 | |
| US201962839346P | 2019-04-26 | 2019-04-26 | |
| US201962839526P | 2019-04-26 | 2019-04-26 | |
| US201962842463P | 2019-05-02 | 2019-05-02 | |
| US201962858331P | 2019-06-07 | 2019-06-07 | |
| US201962860993P | 2019-06-13 | 2019-06-13 | |
| PCT/US2019/048430 WO2020047007A2 (en) | 2018-08-28 | 2019-08-27 | Methods for generating spatially barcoded arrays |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11202102029TA true SG11202102029TA (en) | 2021-03-30 |
Family
ID=69643282
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11202102019UA SG11202102019UA (en) | 2018-08-28 | 2019-08-27 | Method for transposase-mediated spatial tagging and analyzing genomic dna in a biological sample |
| SG11202101944RA SG11202101944RA (en) | 2018-08-28 | 2019-08-27 | Increasing spatial array resolution |
| SG11202102029TA SG11202102029TA (en) | 2018-08-28 | 2019-08-27 | Methods for generating spatially barcoded arrays |
| SG11202102003TA SG11202102003TA (en) | 2018-08-28 | 2019-08-27 | Methods of generating an array |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11202102019UA SG11202102019UA (en) | 2018-08-28 | 2019-08-27 | Method for transposase-mediated spatial tagging and analyzing genomic dna in a biological sample |
| SG11202101944RA SG11202101944RA (en) | 2018-08-28 | 2019-08-27 | Increasing spatial array resolution |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11202102003TA SG11202102003TA (en) | 2018-08-28 | 2019-08-27 | Methods of generating an array |
Country Status (6)
| Country | Link |
|---|---|
| US (6) | US20230323447A1 (en) |
| EP (6) | EP4464793A3 (en) |
| CN (5) | CN113366117B (en) |
| ES (1) | ES3000357T3 (en) |
| SG (4) | SG11202102019UA (en) |
| WO (4) | WO2020047002A1 (en) |
Families Citing this family (138)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2794522C (en) | 2010-04-05 | 2019-11-26 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| US11435358B2 (en) | 2011-06-23 | 2022-09-06 | Board Of Regents, The University Of Texas System | Single molecule peptide sequencing |
| DK3511423T4 (en) | 2012-10-17 | 2024-07-29 | Spatial Transcriptomics Ab | METHODS AND PRODUCT FOR OPTIMIZING LOCALIZED OR SPATIAL DETECTION OF GENE EXPRESSION IN A TISSUE SAMPLE |
| US9868979B2 (en) | 2013-06-25 | 2018-01-16 | Prognosys Biosciences, Inc. | Spatially encoded biological assays using a microfluidic device |
| CA2956925C (en) | 2014-08-01 | 2024-02-13 | Dovetail Genomics, Llc | Tagging nucleic acids for sequence assembly |
| WO2016162309A1 (en) | 2015-04-10 | 2016-10-13 | Spatial Transcriptomics Ab | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| EP3788171B1 (en) | 2018-05-03 | 2023-04-05 | Becton, Dickinson and Company | High throughput multiomics sample analysis |
| JP2021531750A (en) | 2018-07-12 | 2021-11-25 | ボード オブ リージェンツ, ザ ユニバーシティ オブ テキサス システムBoard Of Regents, The University Of Texas System | Molecular neighborhood detection by oligonucleotide |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| CN113767177B (en) | 2018-12-10 | 2025-01-14 | 10X基因组学有限公司 | Generation of capture probes for spatial analysis |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US20240026445A1 (en) * | 2019-01-06 | 2024-01-25 | 10X Genomics, Inc. | Methods for determining a location of a biological analyte in a biological sample |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| CA3131115A1 (en) * | 2019-02-21 | 2020-08-27 | Stratos Genomics, Inc. | Methods, compositions, and devices for solid-state synthesis of expandable polymers for use in single molecule sequencing |
| EP3976820A1 (en) | 2019-05-30 | 2022-04-06 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| US12157124B2 (en) | 2019-11-06 | 2024-12-03 | 10X Genomics, Inc. | Imaging system hardware |
| WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
| EP4055185A1 (en) | 2019-11-08 | 2022-09-14 | 10X Genomics, Inc. | Spatially-tagged analyte capture agents for analyte multiplexing |
| EP4589016A3 (en) * | 2019-11-13 | 2025-10-08 | 10x Genomics, Inc. | Generating capture probes for spatial analysis |
| CN115023734B (en) | 2019-11-22 | 2023-10-20 | 10X基因组学有限公司 | System and method for spatially analyzing analytes using fiducial alignment |
| WO2021119320A2 (en) | 2019-12-11 | 2021-06-17 | 10X Genomics, Inc. | Reverse transcriptase variants |
| CN115038794A (en) | 2019-12-23 | 2022-09-09 | 10X基因组学有限公司 | Compositions and methods for using fixed biological samples in partition-based assays |
| CN114885610B (en) | 2019-12-23 | 2025-09-05 | 10X基因组学有限公司 | Methods for spatial profiling using RNA-templated ligation |
| JP2023511279A (en) | 2020-01-13 | 2023-03-17 | フルーエント バイオサイエンシーズ インコーポレイテッド | Single cell sequencing |
| US12365942B2 (en) | 2020-01-13 | 2025-07-22 | 10X Genomics, Inc. | Methods of decreasing background on a spatial array |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
| US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
| US20210230681A1 (en) | 2020-01-24 | 2021-07-29 | 10X Genomics, Inc. | Methods for spatial analysis using proximity ligation |
| US11821035B1 (en) | 2020-01-29 | 2023-11-21 | 10X Genomics, Inc. | Compositions and methods of making gene expression libraries |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
| US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
| WO2021158925A1 (en) | 2020-02-07 | 2021-08-12 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
| US11835462B2 (en) | 2020-02-11 | 2023-12-05 | 10X Genomics, Inc. | Methods and compositions for partitioning a biological sample |
| US12281357B1 (en) | 2020-02-14 | 2025-04-22 | 10X Genomics, Inc. | In situ spatial barcoding |
| US12399123B1 (en) | 2020-02-14 | 2025-08-26 | 10X Genomics, Inc. | Spatial targeting of analytes |
| WO2021168278A1 (en) * | 2020-02-20 | 2021-08-26 | 10X Genomics, Inc. | METHODS TO COMBINE FIRST AND SECOND STRAND cDNA SYNTHESIS FOR SPATIAL ANALYSIS |
| WO2021168261A1 (en) * | 2020-02-21 | 2021-08-26 | 10X Genomics, Inc. | Capturing genetic targets using a hybridization approach |
| EP4107285B1 (en) | 2020-02-21 | 2024-10-09 | 10X Genomics, Inc. | Methods and compositions for integrated in situ spatial assay |
| US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
| US11926863B1 (en) | 2020-02-27 | 2024-03-12 | 10X Genomics, Inc. | Solid state single cell method for analyzing fixed biological cells |
| US11768175B1 (en) | 2020-03-04 | 2023-09-26 | 10X Genomics, Inc. | Electrophoretic methods for spatial analysis |
| EP4121016A4 (en) | 2020-03-16 | 2024-09-11 | Fluent Biosciences Inc. | MULTIOMIC ANALYSIS IN MONODISPERSE DROPLETS |
| WO2021195169A1 (en) | 2020-03-24 | 2021-09-30 | Fluent Biosciences Inc. | Viral detection using template emulsification |
| EP4136227A1 (en) | 2020-04-16 | 2023-02-22 | 10X Genomics, Inc. | Compositions and methods for use with fixed samples |
| WO2021216708A1 (en) | 2020-04-22 | 2021-10-28 | 10X Genomics, Inc. | Methods for spatial analysis using targeted rna depletion |
| US20230265491A1 (en) * | 2020-05-04 | 2023-08-24 | 10X Genomics, Inc. | Spatial transcriptomic transfer modes |
| US11851700B1 (en) | 2020-05-13 | 2023-12-26 | 10X Genomics, Inc. | Methods, kits, and compositions for processing extracellular molecules |
| WO2021236625A1 (en) | 2020-05-19 | 2021-11-25 | 10X Genomics, Inc. | Electrophoresis cassettes and instrumentation |
| EP4153776B1 (en) | 2020-05-22 | 2025-03-05 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
| EP4414459B1 (en) | 2020-05-22 | 2025-09-03 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| WO2021242834A1 (en) | 2020-05-26 | 2021-12-02 | 10X Genomics, Inc. | Method for resetting an array |
| EP4600376A3 (en) | 2020-06-02 | 2025-10-22 | 10X Genomics, Inc. | Spatial transcriptomics for antigen-receptors |
| WO2021247543A2 (en) | 2020-06-02 | 2021-12-09 | 10X Genomics, Inc. | Nucleic acid library methods |
| US12265079B1 (en) | 2020-06-02 | 2025-04-01 | 10X Genomics, Inc. | Systems and methods for detecting analytes from captured single biological particles |
| CN116234926A (en) * | 2020-06-03 | 2023-06-06 | 中国海洋大学 | Method for analyzing target nucleic acid from cells |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| EP4421186B1 (en) | 2020-06-08 | 2025-08-13 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
| US12435363B1 (en) | 2020-06-10 | 2025-10-07 | 10X Genomics, Inc. | Materials and methods for spatial transcriptomics |
| EP4165207B1 (en) | 2020-06-10 | 2024-09-25 | 10X Genomics, Inc. | Methods for determining a location of an analyte in a biological sample |
| WO2021252747A1 (en) | 2020-06-10 | 2021-12-16 | 1Ox Genomics, Inc. | Fluid delivery methods |
| ES2994976T3 (en) | 2020-06-25 | 2025-02-05 | 10X Genomics Inc | Spatial analysis of dna methylation |
| US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
| US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
| US12209280B1 (en) | 2020-07-06 | 2025-01-28 | 10X Genomics, Inc. | Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis |
| US12241059B2 (en) | 2020-07-15 | 2025-03-04 | Illumina, Inc. | Tiered ligation oligos |
| EP4182462A4 (en) * | 2020-07-17 | 2024-09-25 | The Regents Of The University Of Michigan | MATERIALS AND METHODS FOR LOCALIZED DETECTION OF NUCLEIC ACIDS IN A TISSUE SAMPLE |
| US11802312B2 (en) | 2020-08-10 | 2023-10-31 | Dimension Genomics Inc. | Devices and methods for multi-dimensional genome analysis |
| US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
| CN116457100A (en) * | 2020-09-18 | 2023-07-18 | 10X基因组学有限公司 | Sample processing apparatus and fluid delivery method |
| ES2993269T3 (en) | 2020-09-18 | 2024-12-26 | 10X Genomics Inc | Sample handling apparatus and image registration methods |
| WO2022061152A2 (en) * | 2020-09-18 | 2022-03-24 | 10X Genomics, Inc. | Sample handling apparatus and fluid delivery methods |
| US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
| WO2022067565A1 (en) * | 2020-09-29 | 2022-04-07 | 生物岛实验室 | Spatial omics sequencing method, single cell epitranscriptomic sequencing method, and localization labeling method |
| CN114045332A (en) * | 2020-09-29 | 2022-02-15 | 生物岛实验室 | A single-cell epitranscriptomic sequencing approach |
| US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
| US20240093291A1 (en) * | 2020-12-14 | 2024-03-21 | Cz Biohub Sf, Llc | Spatial genomics with single cell resolution |
| EP4121555A1 (en) | 2020-12-21 | 2023-01-25 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| EP4269615A4 (en) * | 2020-12-25 | 2025-01-08 | Intelligent Healthcare Technology Limited | Biochip for spatial transcriptomic analysis, manufacturing method therefor and application thereof |
| WO2022147296A1 (en) | 2020-12-30 | 2022-07-07 | 10X Genomics, Inc. | Cleavage of capture probes for spatial analysis |
| WO2022147134A1 (en) * | 2020-12-30 | 2022-07-07 | 10X Genomics, Inc. | Molecular arrays and methods for generating and using the arrays |
| US20220314187A1 (en) * | 2020-12-30 | 2022-10-06 | 10X Genomics, Inc. | Methods and compositions for light-controlled surface patterning using a polymer |
| AU2021411520A1 (en) * | 2020-12-30 | 2023-08-10 | Dovetail Genomics, Llc | Methods and compositions for sequencing library preparation |
| EP4274907A4 (en) | 2021-01-08 | 2025-01-15 | Cellanome, Inc. | Devices and methods for analyzing biological samples |
| WO2022152740A1 (en) * | 2021-01-12 | 2022-07-21 | Paris Sciences Et Lettres | Method for spatial mapping and sequencing of cells or organelles |
| EP4421491A3 (en) | 2021-02-19 | 2024-11-27 | 10X Genomics, Inc. | Method of using a modular assay support device |
| ES3008686T3 (en) | 2021-03-18 | 2025-03-24 | 10X Genomics Inc | Multiplex capture of gene and protein expression from a biological sample |
| EP4305196B1 (en) | 2021-04-14 | 2025-04-02 | 10X Genomics, Inc. | Methods of measuring mislocalization of an analyte |
| WO2022226057A1 (en) * | 2021-04-20 | 2022-10-27 | 10X Genomics, Inc. | Methods for assessing sample quality prior to spatial analysis using templated ligation |
| WO2022226372A1 (en) * | 2021-04-23 | 2022-10-27 | Ultima Genomics, Inc. | Systems and methods for spatial reference sequencing |
| EP4313911A4 (en) * | 2021-04-30 | 2025-01-01 | Quantum-si Incorporated | Universal adaptor for sequencing |
| WO2022232571A1 (en) | 2021-04-30 | 2022-11-03 | 10X Genomics, Inc. | Fusion rt variants for improved performance |
| WO2022236054A1 (en) | 2021-05-06 | 2022-11-10 | 10X Genomics, Inc. | Methods for increasing resolution of spatial analysis |
| CN117642633A (en) * | 2021-05-26 | 2024-03-01 | 德克萨斯大学系统董事会 | Methods and systems for single-cell protein analysis |
| WO2022251445A1 (en) * | 2021-05-28 | 2022-12-01 | Fluent Biosciences Inc. | Spatial transcriptomics in pips |
| EP4582555A3 (en) | 2021-06-03 | 2025-10-22 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| MX2023014290A (en) * | 2021-06-11 | 2024-02-27 | Cellanome Inc | Systems and methods for analyzing biological samples. |
| WO2022265965A1 (en) | 2021-06-14 | 2022-12-22 | 10X Genomics, Inc. | Reverse transcriptase variants for improved performance |
| EP4370675B1 (en) * | 2021-08-12 | 2025-10-01 | 10X Genomics, Inc. | Methods, compositions and systems for identifying antigen-binding molecules |
| EP4509614A3 (en) | 2021-09-01 | 2025-05-14 | 10X Genomics, Inc. | Methods, compositions, and kits for blocking a capture probe on a spatial array |
| WO2023059658A1 (en) * | 2021-10-04 | 2023-04-13 | AtlasXomics Inc. | System and methods for high throughput screening of tissue preparation conditions |
| WO2023076345A1 (en) * | 2021-10-26 | 2023-05-04 | 10X Genomics, Inc. | Methods for spatial analysis using targeted rna capture |
| EP4419707A1 (en) | 2021-11-10 | 2024-08-28 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of an analyte in a biological sample |
| WO2023102118A2 (en) | 2021-12-01 | 2023-06-08 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| WO2023114473A2 (en) | 2021-12-16 | 2023-06-22 | 10X Genomics, Inc. | Recombinant reverse transcriptase variants for improved performance |
| EP4441711A1 (en) | 2021-12-20 | 2024-10-09 | 10X Genomics, Inc. | Self-test for pathology/histology slide imaging device |
| CN116376662A (en) * | 2021-12-24 | 2023-07-04 | 映泰科技有限公司 | Biochip for space transcriptome analysis, its preparation method and application |
| CN116694730B (en) * | 2022-02-28 | 2025-02-14 | 南方科技大学 | A method for constructing a single-cell open chromatin and transcriptome co-sequencing library |
| CN114574569B (en) * | 2022-03-28 | 2023-05-16 | 浙江大学 | Genome sequencing kit and sequencing method based on terminal transferase |
| WO2023196526A1 (en) | 2022-04-06 | 2023-10-12 | 10X Genomics, Inc. | Methods for multiplex cell analysis |
| EP4519674A1 (en) | 2022-05-06 | 2025-03-12 | 10X Genomics, Inc. | Analysis of antigen and antigen receptor interactions |
| WO2023225519A1 (en) | 2022-05-17 | 2023-11-23 | 10X Genomics, Inc. | Modified transposons, compositions and uses thereof |
| WO2023250077A1 (en) * | 2022-06-22 | 2023-12-28 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| US20240002932A1 (en) * | 2022-06-29 | 2024-01-04 | 10X Genomics, Inc. | Click chemistry-based dna photo-ligation for manufacturing of high-resolution dna arrays |
| WO2024006799A1 (en) * | 2022-06-29 | 2024-01-04 | 10X Genomics, Inc. | Covalent attachment of splint oligonucleotides for molecular array generation using ligation |
| US20240182959A1 (en) * | 2022-11-21 | 2024-06-06 | Illumina, Inc. | Reversibly cross-linked hydrogels, and methods of using the same for cluster amplification |
| EP4623079A1 (en) | 2022-11-23 | 2025-10-01 | Alida Biosciences, Inc. | Chromatin profiling compositions and methods |
| WO2024124044A1 (en) | 2022-12-07 | 2024-06-13 | The Brigham And Women’S Hospital, Inc. | Compositions and methods targeting sat1 for enhancing anti¬ tumor immunity during tumor progression |
| CN116254611A (en) * | 2022-12-16 | 2023-06-13 | 南方科技大学 | Construction method of multi-sample ultrahigh-flux single-cell transcriptome sequencing library |
| WO2024141901A1 (en) * | 2022-12-28 | 2024-07-04 | Moleculent Ab | Heat-based transfer of reaction products made in situ to a planar support |
| CN116286753A (en) * | 2023-01-06 | 2023-06-23 | 广州达安基因股份有限公司 | Preparation method and application of recombinant type I collagenase and mutant thereof |
| CN117385477B (en) * | 2023-03-10 | 2024-09-20 | 深圳赛陆医疗科技有限公司 | Chip for space transcriptome sequencing, preparation method thereof and space transcriptome sequencing method |
| WO2024192141A1 (en) | 2023-03-13 | 2024-09-19 | Dana-Farber Cancer Institute, Inc. | Treatment of cancers having a drug-resistant mesenchymal cell state |
| CN121039290A (en) * | 2023-03-16 | 2025-11-28 | 生物辐射实验室股份有限公司 | Click chemistry-based barcode encoding |
| EP4435114A1 (en) * | 2023-03-24 | 2024-09-25 | Miltenyi Biotec B.V. & Co. KG | Syntheis of highly multiplexed oligo-barcoded fluorescent beads for single cell seq applications |
| US20240417792A1 (en) * | 2023-06-08 | 2024-12-19 | Illumina, Inc. | Capturing and amplifying polynucleotides, and reusing flowcells, using silica particles |
| WO2024258911A1 (en) | 2023-06-13 | 2024-12-19 | 10X Genomics, Inc. | Sequence specific dna binding proteins |
| WO2025072368A1 (en) * | 2023-09-28 | 2025-04-03 | Illumina, Inc. | Capture and selective release of biological material |
| WO2025081196A1 (en) * | 2023-10-12 | 2025-04-17 | Georgia Tech Research Corporation | Scarless isolation of antigen-specific t cells for car t cells for car t cell manufacturing via dnagated sorting |
| WO2025090912A1 (en) * | 2023-10-26 | 2025-05-01 | 10X Genomics, Inc. | Spatial arrays containing reusable master probes |
| US20250230431A1 (en) * | 2023-12-18 | 2025-07-17 | Genemind Biosciences Co., Ltd. | Spatial chip, method for preparing same, and use thereof |
| WO2025168224A1 (en) * | 2024-02-08 | 2025-08-14 | Leica Microsystems Cms Gmbh | Marker and method for analysing a biological sample |
| WO2025231225A1 (en) * | 2024-05-03 | 2025-11-06 | Place Genomics Corporation | Probe-based analysis of biological molecules |
Family Cites Families (139)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US4968601A (en) * | 1988-02-09 | 1990-11-06 | The United States Of America As Represented By The Dept. Of Health & Human Services | Method for diagnosing latent viral infection |
| US5472881A (en) | 1992-11-12 | 1995-12-05 | University Of Utah Research Foundation | Thiol labeling of DNA for attachment to gold surfaces |
| US5874239A (en) | 1993-07-30 | 1999-02-23 | Affymax Technologies N.V. | Biotinylation of proteins |
| US5610287A (en) | 1993-12-06 | 1997-03-11 | Molecular Tool, Inc. | Method for immobilizing nucleic acid molecules |
| US5512462A (en) | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
| US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
| US5552278A (en) | 1994-04-04 | 1996-09-03 | Spectragen, Inc. | DNA sequencing by stepwise ligation and cleavage |
| US5807522A (en) | 1994-06-17 | 1998-09-15 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for fabricating microarrays of biological samples |
| US5641658A (en) | 1994-08-03 | 1997-06-24 | Mosaic Technologies, Inc. | Method for performing amplification of nucleic acid with two primers bound to a single solid support |
| US5846719A (en) | 1994-10-13 | 1998-12-08 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| US5965443A (en) | 1996-09-09 | 1999-10-12 | Wisconsin Alumni Research Foundation | System for in vitro transposition |
| US5925545A (en) | 1996-09-09 | 1999-07-20 | Wisconsin Alumni Research Foundation | System for in vitro transposition |
| GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
| GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
| AU747242B2 (en) | 1997-01-08 | 2002-05-09 | Proligo Llc | Bioconjugation of macromolecules |
| US5837860A (en) | 1997-03-05 | 1998-11-17 | Molecular Tool, Inc. | Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds |
| US6023540A (en) | 1997-03-14 | 2000-02-08 | Trustees Of Tufts College | Fiber optic sensor with encoded microspheres |
| US6327410B1 (en) | 1997-03-14 | 2001-12-04 | The Trustees Of Tufts College | Target analyte sensors utilizing Microspheres |
| ATE269908T1 (en) | 1997-04-01 | 2004-07-15 | Manteia S A | METHOD FOR SEQUENCING NUCLEIC ACIDS |
| JP2002503954A (en) | 1997-04-01 | 2002-02-05 | グラクソ、グループ、リミテッド | Nucleic acid amplification method |
| US6969488B2 (en) | 1998-05-22 | 2005-11-29 | Solexa, Inc. | System and apparatus for sequential processing of analytes |
| US5919626A (en) | 1997-06-06 | 1999-07-06 | Orchid Bio Computer, Inc. | Attachment of unmodified nucleic acids to silanized solid phase surfaces |
| US7427678B2 (en) | 1998-01-08 | 2008-09-23 | Sigma-Aldrich Co. | Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method |
| AU3567099A (en) * | 1998-04-16 | 1999-11-01 | Packard Bioscience Company | Analysis of polynucleotide sequence |
| US6387632B2 (en) * | 1998-06-11 | 2002-05-14 | Hitachi, Ltd. | Polynucleotide separation method and apparatus therefor |
| WO1999067641A2 (en) | 1998-06-24 | 1999-12-29 | Illumina, Inc. | Decoding of array sensors with microspheres |
| US6159736A (en) | 1998-09-23 | 2000-12-12 | Wisconsin Alumni Research Foundation | Method for making insertional mutations using a Tn5 synaptic complex |
| AR021833A1 (en) | 1998-09-30 | 2002-08-07 | Applied Research Systems | METHODS OF AMPLIFICATION AND SEQUENCING OF NUCLEIC ACID |
| US6391937B1 (en) | 1998-11-25 | 2002-05-21 | Motorola, Inc. | Polyacrylamide hydrogels and hydrogel arrays made from polyacrylamide reactive prepolymers |
| US6355431B1 (en) | 1999-04-20 | 2002-03-12 | Illumina, Inc. | Detection of nucleic acid amplification reactions using bead arrays |
| ATE413467T1 (en) | 1999-04-20 | 2008-11-15 | Illumina Inc | DETECTION OF NUCLEIC ACID REACTIONS ON BEAD ARRAYS |
| US20060275782A1 (en) * | 1999-04-20 | 2006-12-07 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
| AU775043B2 (en) | 1999-08-02 | 2004-07-15 | Wisconsin Alumni Research Foundation | Mutant Tn5 transposase enzymes and method for their use |
| US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
| US7244559B2 (en) | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US6770441B2 (en) | 2000-02-10 | 2004-08-03 | Illumina, Inc. | Array compositions and methods of making same |
| US7001792B2 (en) | 2000-04-24 | 2006-02-21 | Eagle Research & Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| GB0018120D0 (en) | 2000-07-24 | 2000-09-13 | Fermentas Ab | Nuclease |
| AR031640A1 (en) | 2000-12-08 | 2003-09-24 | Applied Research Systems | ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS IN A SOLID SUPPORT |
| US7297778B2 (en) * | 2001-07-25 | 2007-11-20 | Affymetrix, Inc. | Complexity management of genomic DNA |
| US20040019005A1 (en) * | 2001-10-01 | 2004-01-29 | Jeffrey Van Ness | Methods for parallel measurement of genetic variations |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| WO2003070294A2 (en) | 2002-01-16 | 2003-08-28 | Mayo Foundation For Medical Education And Research | Method and apparatus for image-guided therapy |
| US20040002090A1 (en) | 2002-03-05 | 2004-01-01 | Pascal Mayer | Methods for detecting genome-wide sequence variations associated with a phenotype |
| WO2003101972A1 (en) | 2002-05-30 | 2003-12-11 | The Scripps Research Institute | Copper-catalysed ligation of azides and acetylenes |
| EP3795577A1 (en) | 2002-08-23 | 2021-03-24 | Illumina Cambridge Limited | Modified nucleotides |
| KR20040062847A (en) * | 2003-01-03 | 2004-07-09 | 삼성전자주식회사 | Method for replicating nucleic acid array |
| GB0302058D0 (en) * | 2003-01-29 | 2003-02-26 | Univ Cranfield | Replication of nucleic acid arrays |
| US7083980B2 (en) | 2003-04-17 | 2006-08-01 | Wisconsin Alumni Research Foundation | Tn5 transposase mutants and the use thereof |
| US20050031176A1 (en) | 2003-08-08 | 2005-02-10 | Hertel Sarah R. | Method and apparatus of multi-modality image fusion |
| GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
| US7259258B2 (en) | 2003-12-17 | 2007-08-21 | Illumina, Inc. | Methods of attaching biological compounds to solid supports using triazine |
| EP3175914A1 (en) | 2004-01-07 | 2017-06-07 | Illumina Cambridge Limited | Improvements in or relating to molecular arrays |
| US7608434B2 (en) | 2004-08-04 | 2009-10-27 | Wisconsin Alumni Research Foundation | Mutated Tn5 transposase proteins and the use thereof |
| EP1828412B2 (en) | 2004-12-13 | 2019-01-09 | Illumina Cambridge Limited | Improved method of nucleotide detection |
| GB0427236D0 (en) | 2004-12-13 | 2005-01-12 | Solexa Ltd | Improved method of nucleotide detection |
| NZ563193A (en) * | 2005-05-09 | 2010-05-28 | Ono Pharmaceutical Co | Human monoclonal antibodies to programmed death 1(PD-1) and methods for treating cancer using anti-PD-1 antibodies alone or in combination with other immunotherapeutics |
| US8623628B2 (en) | 2005-05-10 | 2014-01-07 | Illumina, Inc. | Polymerases |
| ES2434915T3 (en) | 2005-06-20 | 2013-12-18 | Advanced Cell Diagnostics, Inc. | Multiplex nucleic acid detection |
| GB0514936D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Preparation of templates for nucleic acid sequencing |
| GB0522310D0 (en) | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
| US20080009420A1 (en) | 2006-03-17 | 2008-01-10 | Schroth Gary P | Isothermal methods for creating clonal single molecule arrays |
| WO2007111937A1 (en) * | 2006-03-23 | 2007-10-04 | Applera Corporation | Directed enrichment of genomic dna for high-throughput sequencing |
| EP3536396B1 (en) | 2006-08-07 | 2022-03-30 | The President and Fellows of Harvard College | Fluorocarbon emulsion stabilizing surfactants |
| WO2008022332A2 (en) * | 2006-08-18 | 2008-02-21 | Board Of Regents, The University Of Texas System | System, method and kit for replicating a dna array |
| US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
| US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| EP4134667B1 (en) | 2006-12-14 | 2025-11-12 | Life Technologies Corporation | Apparatus for measuring analytes using fet arrays |
| US20100055733A1 (en) | 2008-09-04 | 2010-03-04 | Lutolf Matthias P | Manufacture and uses of reactive microcontact printing of biomolecules on soft hydrogels |
| US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| DE102009012169B3 (en) * | 2009-03-06 | 2010-11-04 | Albert-Ludwigs-Universität Freiburg | Apparatus and method for making a replica or derivative from an array of molecules and applications thereof |
| US20130171621A1 (en) | 2010-01-29 | 2013-07-04 | Advanced Cell Diagnostics Inc. | Methods of in situ detection of nucleic acids |
| EP2354242A1 (en) * | 2010-02-03 | 2011-08-10 | Epiontis GmbH | Assay for determining the type and/or status of a cell based on the epigenetic pattern and the chromatin structure |
| CA2794522C (en) | 2010-04-05 | 2019-11-26 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| US8951781B2 (en) | 2011-01-10 | 2015-02-10 | Illumina, Inc. | Systems, methods, and apparatuses to image a sample for biological or chemical analysis |
| GB201106254D0 (en) * | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| US8728987B2 (en) * | 2011-08-03 | 2014-05-20 | Bio-Rad Laboratories, Inc. | Filtering small nucleic acids using permeabilized cells |
| CA2859660C (en) | 2011-09-23 | 2021-02-09 | Illumina, Inc. | Methods and compositions for nucleic acid sequencing |
| WO2013096838A2 (en) * | 2011-12-22 | 2013-06-27 | Ibis Biosciences, Inc. | Systems and methods for isolating nucleic acids |
| NO2694769T3 (en) * | 2012-03-06 | 2018-03-03 | ||
| JP6159391B2 (en) | 2012-04-03 | 2017-07-05 | イラミーナ インコーポレーテッド | Integrated read head and fluid cartridge useful for nucleic acid sequencing |
| US9012022B2 (en) | 2012-06-08 | 2015-04-21 | Illumina, Inc. | Polymer coatings |
| US8895249B2 (en) | 2012-06-15 | 2014-11-25 | Illumina, Inc. | Kinetic exclusion amplification of nucleic acid libraries |
| CA2881685C (en) | 2012-08-14 | 2023-12-05 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| US20140378345A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US9783841B2 (en) | 2012-10-04 | 2017-10-10 | The Board Of Trustees Of The Leland Stanford Junior University | Detection of target nucleic acids in a cellular sample |
| DK3511423T4 (en) * | 2012-10-17 | 2024-07-29 | Spatial Transcriptomics Ab | METHODS AND PRODUCT FOR OPTIMIZING LOCALIZED OR SPATIAL DETECTION OF GENE EXPRESSION IN A TISSUE SAMPLE |
| WO2014085725A1 (en) | 2012-11-27 | 2014-06-05 | Tufts University | Biopolymer-based inks and use thereof |
| US9512422B2 (en) | 2013-02-26 | 2016-12-06 | Illumina, Inc. | Gel patterned surfaces |
| US10138509B2 (en) | 2013-03-12 | 2018-11-27 | President And Fellows Of Harvard College | Method for generating a three-dimensional nucleic acid containing matrix |
| US20160019337A1 (en) * | 2013-03-15 | 2016-01-21 | Htg Molecular Diagnostics, Inc. | Subtyping lung cancers |
| US9868979B2 (en) | 2013-06-25 | 2018-01-16 | Prognosys Biosciences, Inc. | Spatially encoded biological assays using a microfluidic device |
| US20150000854A1 (en) | 2013-06-27 | 2015-01-01 | The Procter & Gamble Company | Sheet products bearing designs that vary among successive sheets, and apparatus and methods for producing the same |
| EP3039158B1 (en) * | 2013-08-28 | 2018-11-14 | Cellular Research, Inc. | Massively parallel single cell analysis |
| US10041949B2 (en) | 2013-09-13 | 2018-08-07 | The Board Of Trustees Of The Leland Stanford Junior University | Multiplexed imaging of tissues using mass tags and secondary ion mass spectrometry |
| SG11201604965RA (en) * | 2013-12-20 | 2016-07-28 | Univ Singapore | Differentiation therapy with cd137 ligand agonists |
| WO2015161173A1 (en) | 2014-04-18 | 2015-10-22 | William Marsh Rice University | Competitive compositions of nucleic acid molecules for enrichment of rare-allele-bearing species |
| SG10202005892SA (en) | 2014-06-06 | 2020-07-29 | Herlev Hospital | Determining antigen recognition through barcoding of mhc multimers |
| WO2015188839A2 (en) | 2014-06-13 | 2015-12-17 | Immudex Aps | General detection and isolation of specific cells by binding of labeled molecules |
| WO2015200893A2 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| US10179932B2 (en) | 2014-07-11 | 2019-01-15 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
| US20170349940A1 (en) * | 2014-09-26 | 2017-12-07 | Two Pore Guys, Inc. | Targeted Sequence Detection by Nanopore Sensing of Synthetic Probes |
| US20160108458A1 (en) | 2014-10-06 | 2016-04-21 | The Board Of Trustees Of The Leland Stanford Junior University | Multiplexed detection and quantification of nucleic acids in single-cells |
| EP3262192B1 (en) | 2015-02-27 | 2020-09-16 | Becton, Dickinson and Company | Spatially addressable molecular barcoding |
| EP4180535A1 (en) * | 2015-03-30 | 2023-05-17 | Becton, Dickinson and Company | Methods and compositions for combinatorial barcoding |
| WO2016162309A1 (en) | 2015-04-10 | 2016-10-13 | Spatial Transcriptomics Ab | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| US10059990B2 (en) | 2015-04-14 | 2018-08-28 | Massachusetts Institute Of Technology | In situ nucleic acid sequencing of expanded biological samples |
| EP3283641B1 (en) | 2015-04-14 | 2019-11-27 | Koninklijke Philips N.V. | Spatial mapping of molecular profiles of biological tissue samples |
| WO2017015099A1 (en) | 2015-07-17 | 2017-01-26 | Nanostring Technologies, Inc. | Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue |
| CA3242290A1 (en) | 2015-07-27 | 2017-02-02 | Illumina, Inc. | Spatial mapping of nucleic acid sequence information |
| US10364457B2 (en) | 2015-08-07 | 2019-07-30 | Massachusetts Institute Of Technology | Nanoscale imaging of proteins and nucleic acids via expansion microscopy |
| CA2994957A1 (en) | 2015-08-07 | 2017-02-16 | Massachusetts Institute Of Technology | Protein retention expansion microscopy |
| EP3882357B1 (en) | 2015-12-04 | 2022-08-10 | 10X Genomics, Inc. | Methods and compositions for nucleic acid analysis |
| US20170241911A1 (en) | 2016-02-22 | 2017-08-24 | Miltenyi Biotec Gmbh | Automated analysis tool for biological specimens |
| JP7025340B2 (en) | 2016-02-26 | 2022-02-24 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | Multiplexed single molecule RNA visualization using a two-probe proximity ligation system |
| US20170253918A1 (en) | 2016-03-01 | 2017-09-07 | Expansion Technologies | Combining protein barcoding with expansion microscopy for in-situ, spatially-resolved proteomics |
| EP3868879A1 (en) * | 2016-03-10 | 2021-08-25 | The Board of Trustees of the Leland Stanford Junior University | Transposase-mediated imaging of the accessible genome |
| WO2017161251A1 (en) | 2016-03-17 | 2017-09-21 | President And Fellows Of Harvard College | Methods for detecting and identifying genomic nucleic acids |
| US20180052081A1 (en) | 2016-05-11 | 2018-02-22 | Expansion Technologies | Combining modified antibodies with expansion microscopy for in-situ, spatially-resolved proteomics |
| US10894990B2 (en) * | 2016-05-17 | 2021-01-19 | Shoreline Biome, Llc | High throughput method for identification and sequencing of unknown microbial and eukaryotic genomes from complex mixtures |
| EP3472359B1 (en) | 2016-06-21 | 2022-03-16 | 10X Genomics, Inc. | Nucleic acid sequencing |
| AU2017302300B2 (en) | 2016-07-27 | 2023-08-17 | The Board Of Trustees Of The Leland Stanford Junior University | Highly-multiplexed fluorescent imaging |
| CN118389650A (en) | 2016-08-31 | 2024-07-26 | 哈佛学院董事及会员团体 | Methods for generating nucleic acid sequence libraries for detection by fluorescent in situ sequencing |
| CN118853848A (en) | 2016-08-31 | 2024-10-29 | 哈佛学院董事及会员团体 | Methods for combining detection of biomolecules into a single assay using fluorescent in situ sequencing |
| US11505819B2 (en) | 2016-09-22 | 2022-11-22 | William Marsh Rice University | Molecular hybridization probes for complex sequence capture and analysis |
| SG10202012440VA (en) | 2016-10-19 | 2021-01-28 | 10X Genomics Inc | Methods and systems for barcoding nucleic acid molecules from individual cells or cell populations |
| GB201619458D0 (en) | 2016-11-17 | 2017-01-04 | Spatial Transcriptomics Ab | Method for spatial tagging and analysing nucleic acids in a biological specimen |
| US10656144B2 (en) | 2016-12-02 | 2020-05-19 | The Charlotte Mecklenburg Hospital Authority | Immune profiling and minimal residue disease following stem cell transplantation in multiple myeloma |
| CN118345145A (en) | 2016-12-09 | 2024-07-16 | 乌尔蒂维尤股份有限公司 | Improved methods for multiplexed imaging using labeled nucleic acid imaging agents |
| WO2018136856A1 (en) | 2017-01-23 | 2018-07-26 | Massachusetts Institute Of Technology | Multiplexed signal amplified fish via splinted ligation amplification and sequencing |
| CN120210336A (en) | 2017-10-06 | 2025-06-27 | 10X基因组学有限公司 | RNA templated ligation |
| WO2019075091A1 (en) | 2017-10-11 | 2019-04-18 | Expansion Technologies | Multiplexed in situ hybridization of tissue sections for spatially resolved transcriptomics with expansion microscopy |
| CN114807306A (en) | 2017-12-08 | 2022-07-29 | 10X基因组学有限公司 | Methods and compositions for labeling cells |
-
2019
- 2019-08-27 CN CN201980071274.0A patent/CN113366117B/en active Active
- 2019-08-27 WO PCT/US2019/048425 patent/WO2020047002A1/en not_active Ceased
- 2019-08-27 WO PCT/US2019/048427 patent/WO2020047004A2/en not_active Ceased
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- 2019-08-27 WO PCT/US2019/048434 patent/WO2020047010A2/en not_active Ceased
- 2019-08-27 EP EP19773221.7A patent/EP3844307A1/en active Pending
- 2019-08-27 CN CN201980071234.6A patent/CN113286893A/en active Pending
- 2019-08-27 US US18/023,257 patent/US20230323447A1/en active Pending
- 2019-08-27 SG SG11202102029TA patent/SG11202102029TA/en unknown
- 2019-08-27 WO PCT/US2019/048430 patent/WO2020047007A2/en not_active Ceased
- 2019-08-27 US US17/271,995 patent/US20210332425A1/en active Pending
- 2019-08-27 ES ES19765928T patent/ES3000357T3/en active Active
- 2019-08-27 EP EP19773222.5A patent/EP3844305A2/en active Pending
- 2019-08-27 CN CN202511001938.XA patent/CN120775968A/en active Pending
- 2019-08-27 EP EP19765928.7A patent/EP3844304B1/en active Active
- 2019-08-27 US US17/271,979 patent/US20210332424A1/en active Pending
- 2019-08-27 SG SG11202102003TA patent/SG11202102003TA/en unknown
-
2020
- 2020-05-18 US US16/876,709 patent/US20210010070A1/en not_active Abandoned
- 2020-05-18 US US16/876,682 patent/US20200407781A1/en not_active Abandoned
-
2025
- 2025-01-14 US US19/020,235 patent/US20250146057A1/en active Pending
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