RU2760855C1 - Method for experimental modeling of echinococcal cyst - Google Patents

Abstract

FIELD: medicine; experimental surgery.

SUBSTANCE: section of the peritoneum with intra-abdominal fascia is separated from the anterior abdominal wall, forming a flap on a feeding leg. Next, a daughter bubble of echinococcus is placed on the formed flap. Free edges of the flap are sewn over the bubble with the peritoneal surface outward, forming a closed cavity.

EFFECT: method allows one to bring the model of an echinococcal cyst closer to its clinical course, perform the operation in one step, reproduce the cyst in less time, and it can be used to study an echinococcal cyst in the abdominal cavity.

1 cl, 2 dwg, 1 ex

Description

The invention relates to medicine, namely to the field of experimental surgery, and can be used to model an echinococcal cyst of the abdominal cavity.

In the literature, a method is known for modeling solitary liver echinococcosis in an experiment, which consists in implanting a daughter echinococcal cyst into the cavity, which is formed by bringing together two purse-string sutures of all five lobes of the liver of white rats. (RF Patent No. 2222052, IPC G09B 23/28, 2000.01. echinococcosis of the liver in experiment Applicant: Dagestan State Medical Academy).

A known method of reproduction of echinococcosis, which consists in the introduction of embryonic elements intraperitoneally (Lubinsky GA - Canad J Zool - 1960, vol. 38 - p. 1177-1185) directly into the liver parenchyma or into the vessels that bring blood to the organ (Ruzakov V.A., Pavlov V.V., Kovalenko F.P. Medical parasitology - 1987, 5 - pp. 17-19; Kovalenko F.P., Ballad N.Ye., Dzhabarov V.I. Method of modeling lavral alveococcosis. 991485. USSR. Bulletin of Discovery. Inventions. 1983. 3, p. 2).

The disadvantages of these methods are:

- the impossibility of creating a given size of the cavity and the implementation of dynamic control over the cavity of the created cyst,

- long term modeling of the cyst,

- possible subcapsular ruptures of the liver, leading to bleeding and bile leakage,

- the likelihood of seeding with echinococcosis of the abdominal cavity.

- with the introduction of the germ elements of echinococcus into the liver parenchyma, obstruction of large vessels and organ infarction are possible.

As a prototype, a method of creating an echinococcal cyst by implanting daughter bubbles into the fibrous cavity of the liver formed around a foreign body was chosen (Omarov M.M., in the book Questions of diagnosis and surgical treatment of echinococcosis. 1979, - pp. 131-134; Omarov M.M. Diss. Candidate of medical sciences, Baku, 1987, p. 67-85). The method is as follows: the experimental animals are operated twice. During the first operation, an inflatable rubber balloon is implanted into the liver, around which a fibrous capsule is gradually formed. After 40 days, during the second operation, the balloon is removed and a simulated fibrous cavity is found in the liver, into which a living daughter bladder of echinococcus is lowered in compliance with asepsis of the appropriate size. For further studies, the animals are operated on for the third time.

The disadvantages of this method are:

- "open" traumatic way of modeling,

- multi-stage technique,

- impossibility of dynamic assessment of the volume of a simulated cyst,

- the need for a second operation to remove the balloon and implant a daughter bladder,

- long term modeling of an echinococcal cyst.

The proposed invention is aimed at improving the method for modeling the echinococcal cyst of the abdominal cavity. The specified technical result is achieved by the fact that a section of the peritoneum with an intra-abdominal fascia is dissected from the anterior abdominal wall, forming a flap on the feeding leg, then a daughter echinococcus bubble is placed on the formed flap, the free edges of the flap are sutured above it with the peritoneal surface outward, forming a closed cavity.

The proposed method is illustrated by drawings, where; fig. 1 - laparotomy cutting out a peritoneal flap with an intra-abdominal fascia on the feeding leg, where:

1 - Edges of the formed flap

2 - Peritoneal flap with intra-abdominal fascia on the feeding pedicle

3 - Abdominal wall

4 - Laparotomic wound

fig. 2 - the formation of a closed cavity by stitching the free edges of the formed flap with the peritoneal surface outside, where:

3 - Abdominal wall

4 - Laparotomic wound

5 - Cyst model

6 - Daughter bladder of echinococcus

The method is carried out as follows: after a midline laparotomy, a section of the peritoneum with an intra-abdominal fascia on the feeding leg is dissected from the anterior abdominal wall (Fig. 1). After that, the daughter bladder of echinococcus is implanted, followed by implantation of the daughter bladder of echinococcus into the cavity, which is formed by stitching the free edges of the formed flap with the peritoneal surface outside (Fig. 2), thereby forming an echinococcal cyst.

This method was tested on 4 rabbits of the chinchilla breed, aged 0.5-1.5 years and weighing 1.5-2.0 kg in accordance with the "International Recommendations for Biomedical Research Using Laboratory Animals"

Example. Chinchilla rabbit, weight - 2.0 kg.

Below are the results of testing.

After premedication, a midline laparotomy was performed under rometar-zoletil anesthesia. A site was found for performing a model of an echinococcal cyst, a site of the peritoneum and intra-abdominal fascia was prepared from the anterior abdominal wall with a scalpel, and a flap was formed (Fig. 1). Next, we place the daughter bladder of the echinococcus on the formed flap and sew over it the free edges of the formed flap, a closed cavity is formed (Fig. 2) with the peritoneal surface outside (model of the echinococcal cyst). After that, the wound was sutured tightly.

The proposed method improves the method for modeling the echinococcal cyst of the abdominal cavity:

- providing the possibility of modeling the echinococcal cyst of the abdominal cavity,

- approximation of the echinococcal cyst model to its clinical course,

- performing the operation in one step,

- reproduction of the cyst in less time,

- technically easy to implement,

- the possibility of using for the study of the echinococcal cyst of the abdominal cavity.

Claims (1)

A method for experimental modeling of an echinococcal cyst, including the creation of an echinococcal cyst by performing a median laparotomy, characterized in that a section of the peritoneum with an intra-abdominal fascia is dissected from the anterior abdominal wall, forming a flap on the feeding leg, then a daughter bubble of an echinococcus is placed on the formed flap, the free edges are sutured over it flap with the peritoneal surface outward, forming a closed cavity.

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