RU2606849C1 - Method of preventing and treating gastrointestinal diseases in calves - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: invention relates to veterinary science and is intended for preventing and treating gastrointestinal diseases in calves. Method involves using mixture of water-alcohol infusion of purple echinacea herb and inula rootstock in preparation on basis of 70 % of ethyl alcohol and water solution of silver, dissolved in boiled water. Mixture is administered to calves orally 20–30 minutes before feeding twice a day in morning and evening for no more than 10 days, in dose of 0.10–0.12 ml per 1 kg of body weight. Water-alcohol infusion diluted with boiled water in amount of 130–150 ml per 180–220 kg of animal's weight is prepared as follows: 2 parts of purple echinacea herb, 1 part of inula rootstock and 1 part of calendula flowers are mixed with 70 vol% of ethanol in ratio 1:5 and settled for 5-9 days at temperature of 36–44 °C. Produced tincture is brought to 30 % concentration with distilled water, and water solution of silver is introduced into tincture in dose of no more than 200 ml, which is not more than 8 mg of silver ions with 100–120 ml of boiled water per 30–40 kg of body weight.

EFFECT: disclosed method enables more effective prevention and treatment of gastrointestinal diseases in calves.

1 cl, 2 tbl, 1 ex

Description

The invention relates to veterinary medicine, in particular veterinary pharmacology.

A known method for the prevention and treatment of gastrointestinal diseases (see RF patent No. 2404728, class A61K 31/00, 2010), including the introduction of a drug containing ASD F-2, succinic acid, vitamins and distilled water.

The closest technical solution is a method for the prevention of immunodeficiency in newborn calves (see RF patent No. 2349332, class A61K 36/00 (2006.01) and A61K 33/38 (2006.01)), including the use of a mixture of water-alcohol tincture from the herb Echinacea purpurea and rhizomes of elecampane, prepared on the basis of 70% ethyl alcohol and diluted with boiled water in an aqueous solution of silver, which the animal is given orally 20-30 minutes before feeding.

A disadvantage of the known proposal is limited action and not effective enough.

The technical result is to increase the effectiveness of prevention and treatment.

The technical result is achieved in that in a method for the prevention and treatment of gastrointestinal diseases in calves, comprising the use of a mixture of water-alcohol tincture from the herb Echinacea purpurea and rhizome of elecampane, prepared with 70% ethyl alcohol and an aqueous silver solution diluted with boiled water, which the animal give orally 20-30 minutes before feeding according to the invention, calves are given twice a day in the morning and evening for no more than 10 days, at a dose of 0.10-0.12 ml per 1 kg of animal weight, diluted with boiled water in an amount of 130-150 ml per 180-220 kg of the animal’s mass of water-alcohol tincture, which is prepared as follows: 2 parts of purple coneflower herb, 1 part of elecampane rhizome and 1 part of calendula officinalis flowers are mixed with 70 vol.% ethyl alcohol in the ratio 1: 5 and insist for 5-9 days at a temperature of 36-44 ° C and the resulting tincture is adjusted to 30% concentration with distilled water, and an aqueous silver solution is introduced into the tincture in a dose of not more than 200 ml, which is not more than 8 mg of ions silver with 100-120 ml of boiled water per 30-40 kg of animal weight st.

The production name of the herbal preparation obtained for the implementation of the indicated method for the prevention and treatment of gastrointestinal diseases is the word formed from the words calendula, elecampane, echinacea and silver ions, included in the form of a water-alcohol tincture - “Kargdekhin”.

The novelty of the proposed proposal is due to the fact that due to the combined use of cargdechin (a water-alcohol tincture from Echinacea purpurea grass, elecampane rhizomes and calendula flowers with an aqueous silver solution), the prevention and treatment of gastrointestinal diseases in calves is enhanced.

A method for the prevention and treatment of gastrointestinal diseases is as follows: calves are given orally twice a day in the morning and evening for no more than 10 days, 20-30 minutes before feeding at a dose of 0.10-0.12 ml per 1 kg of body weight animal diluted with 130-150 ml of boiled water per 180-220 kg of animal weight water-alcohol tincture with silver ions, purple echinacea grass, elecampane rhizome and calendula flowers, having the working name "Kargdekhin", with the following ratio of plant materials - 2: 1: 1, and insist for 5-9 days at t mperature 36-44 ° C, and aqueous solution of silver introduced into the infusion in a dose of not more than 200 ml, which is no more than 8 mg of silver ions with 100-120 ml of water for 30-40 kg animal weight.

The mechanism of action used in the method of the drug is based on the chemical composition of biologically active substances that are part of the plant material used for its preparation.

The aqueous solution of silver used was obtained in a uniform electric field between the silver electrodes (Cp-9.999). The set parameters of the mode (distance between the electrodes, temperature of the electrolysis process, current strength and voltage) ensure the transfer of silver ions into the distillate with the formation of a solvate shell of dipole water molecules around them. The accumulation (concentration) in the liquid of a kind of "attached" to the water molecules of silver ions is produced in a moving fluid stream, which is in the periodic braking mode. The resulting aqueous solution of silver with a wide spectrum of action, in appearance it is a colorless and odorless liquid with an acidic taste, has a high microbicidal activity of the substance at a concentration of silver ions from 0.001 mg / l, which is significantly lower than the permissible sanitary norm (0.01 g / l ), against cultures of Candida albicans, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli. The stable microbicidal properties of silver ions are enhanced by its acid reaction.

The aqueous silver solution used is developed in accordance with TU 9154-001-10073810-2002, “Chemically pure hydrated silver concentrate” and is produced by Kubanagrotok LLC.

Echinacea purpurea (Echinacea purpurea) in its composition contains the substance echinacin and hydroxycinnamic acids, which stimulate the body to produce its own interferon - an important protein that blocks the division of RNA and DNA viruses. Stimulates the activity of T cells, which are an important mediator in the body's immune system. It stimulates the adrenal cortex, increasing metabolic processes, while not causing its depletion. It also has antibacterial, antifungicidal and antiviral properties.

Elecampane high (Inula helenium L.) contains essential oil (up to 3%), consisting of gelenin, alanthol, proazulene and a mixture of sesquiterpenic lactones (alantolactone, isoalantolactone, dihydroalantolactone), polysaccharides - inulin (up to 40-45%), inulinine, pseudoinulin; traces of alkaloids, saponins, resins, gums, pigments, vitamin E, fatty oil, mucous membranes and bitter substances. Elecampane high has an immunostimulating, general strengthening, secretolytic, enhancing the function of the sex glands (males and females), anti-inflammatory, antimicrobial, antihypoxic, diuretic, sedative, expectorant, analgesic, enveloping, astringent, reparative, sweating, sweating.

Calendula officinalis (Calendula officinalis L.) in flower baskets contains carotenoids and flavonoids (carotene, lycopene, violaxanthin, citraxanthin, rubixanthin, flavoxanthin, flavochrom). Calendula inflorescences also contain polysaccharides, polyphenols, resins (3.4%), mucus (2.5%), nitrogen-containing mucus (1.5%), organic acids (malic, ascorbic and traces of salicylic). In the aerial parts of the plant, up to 10% of the bitter substance of calendula was found, photophytoncidal properties are due to the presence of essential oil. Calendula officinalis has the same properties as elecampane, as well as hypotensive, cardiotonic, anticoagulant, desensitizing.

The drug Kargdekhin based on echinacea purpurea, elecampane rhizome and calendula flowers does not give side effects, it is simple in the technology of preparation and method of use, in therapeutic doses it is not toxic.

For its manufacture, non-scarce, environmentally friendly raw materials are used, which provides economic benefits. The active principle of Kargdekhin is hydroxycinnamic acids, sesquiterpene lactones, inulin, carotenoids, flavonoids and a number of other compounds that make up the tincture obtained from the claimed herbs. These substances play an important role in the manifestation of the immunostimulating properties of the plant. "Kargdekhin" normalizes factors of nonspecific resistance of the body, the activity of immunocompetent cells stimulates metabolic processes.

The drug has a general strengthening, secretolytic, enhancing the function of the endocrine glands, anti-inflammatory, antimicrobial, antispasmodic, hypotensive, cardiotonic, antihypoxic, diuretic, immunostimulating, sedative, anticoagulant, analgesic, voluptuous, antipyretic, voluptuous, antipyretic, voluptuous, antipyretic.

The drug Kargdekhin based on Echinacea purpurea, elecampane rhizome and calendula flowers was used daily 2 times a day for no more than - 10 days until complete recovery. If the timing of the use of the drug on heifers was less than 10 days, then this reduced its prophylactic effect on the animal's body, since during this time the biologically active components did not stimulate the lymphatic system of the body (i.e., the activity of T and B lymphocytes did not increase) and the metabolism was not activated. Using the drug for more than 10 days did not increase its biological activity and made this treatment more expensive.

When using the drug on calves, it must be given to the animal 20-30 minutes before feeding. If the drug is used earlier than 20 minutes before the feed intake, then this time did not ensure its absorption of biologically active substances before the feed intake, as a result, the effectiveness of the drug decreased. If the drug is used later than 30 minutes before the feed intake, the effectiveness of the drug also decreased, since the calf’s body did not have time to adapt and the assimilation of biologically active substances did not occur at the proper level. For optimal effect from the use of the inventive preparation, it is necessary to apply 25 minutes before the start of feeding.

The drug must be used on calves at a dose of 0.10-0.12 ml per 1 kg of animal weight. If the dosage of the drug was less than 0.1 ml per 1 kg of calf weight, then a longer period of use is required.

If the dosage of the drug was more than 0.12 ml per 1 kg of calf weight, the effect remains the same that is economically disadvantageous, since the drug is overused and the cost of the resulting product increases.

An example of a specific implementation of the method of prevention and treatment of gastrointestinal diseases in calves

Pre-prepared tincture based on the collection of herbs Echinacea purpurea, rhizome of elecampane and calendula flowers. Raw materials were purchased at the enterprise of the North Caucasian AIA VILAR. Dried raw materials were crushed, rhizomes, stems, leaves - up to 3 mm, flowers - up to 5 mm, which were mixed in certain mass ratios: 2 parts of purple coneflower, 1 part of elecampane rhizome, 1 part of calendula flowers. The technological process for the preparation of preparations consists of the following stages: mixing the collection of purple coneflower, elecampane rhizome and calendula flowers with 70 vol.% Ethyl rectified alcohol in a ratio of 1: 5. To do this, take 1 part of the raw material, add 5 parts of 70 vol.% Ethyl alcohol (i.e. at a dilution of 1: 5), mix, place in a dark glass dish, close tightly and place in a thermostat at a temperature of 37 ° C for 14 days, periodically mixed. Then it was filtered through a paper filter, after pressing the raw materials well. The resulting tincture was brought to 30% concentration with distilled water, then it was stirred, insisted for 24 hours for better diffusion and an aqueous silver solution was introduced into the tincture in a dose of not more than 200 ml, which is not more than 8 mg of silver ions, with 100-120 ml of boiled water per 30-40 kg of animal mass. During long-term storage (for three years), the drug did not change its properties (Table 1).

In appearance, the phytopreparation is a dark brown liquid with a specific herbal odor and taste, containing 1.0 ± 0.1% hydroxycinnamic acids, essential oils - 3.5%, inulin polysaccharide - 42%, vitamin E, ascorbic acid.

To confirm the effectiveness of the proposed treatment, 3 groups of 25 calves were equipped, one of which was a control, where the calves were not given drugs, in the 2nd group, the calves were given tactivin in a dose of 6.7 × 10 ^-4 ml, and in The 3rd calves were given kalendekhin - 3-10 ^-3 ml.

As a result, it was found that the use of Cargdechin helped to prevent dyspepsia in calves. So, from 25 calves in the control group, 20 sick animals were identified, which amounted to 80% of the total number of animals. The use of tactivin helped to reduce the disease by 76% compared with the control group of animals.

Cargdechin was administered to calves orally for no more than 10 days, twice a day, 20-30 minutes before feeding at a dose of 0.10-0.12 ml per 1 kg of animal weight, diluted with 180-150 ml of boiled water for 180-220 kg of animal mass, after which the morphological, biochemical and immunological parameters of the blood of animals were examined. Tactivin was administered subcutaneously 0.02 ml / kg for 3 days.

Studies of morphological indicators showed that in animals treated with tactivin, the number of red blood cells increased by 9%, and kargdekhin increased by 16% in relation to the control group; white blood cells in the first experimental group - by 15%, and in the second experimental group - by 16%. At the same time, there was a decrease in the number of lymphocytes in the experimental groups (by 11% and 14%, respectively), due to an increase in segmented neutrophils (1.3 times and 1.4 times, respectively), relative to the control group of animals (Table 2).

During biochemical studies of the blood serum of heifers, the following data were obtained: the total protein in animals of the control group was 71.0 g / l, in the first experimental group this indicator was higher than the control group by 22%, in the second experimental group - by 27%.

The blood glucose content in the control group was 5.36 mmol / L, in the heifers of the first experimental group - 5.89 mmol / L, which is 9% higher than in the control, in the second experimental group - 6.80 mmol / L, or 27% higher than in control.

The urea content in the control was 4.32 mmol / L, in the first experimental group - 3.05 mmol / L (29% lower relative to the control), in the second experimental group - 2.65 mmol / L (39% lower relative to the control ) The cholesterol content in all groups was approximately the same.

From the results obtained, it can be concluded that the use of tactivin and kargdekhin stimulated metabolic processes in the body and increased the natural resistance of animals.

A study of bacterial phagocytosis showed that the percentage of actively phagocytic neutrophils in the control group of animals was 43%, in the first and second experimental groups - 50% and 55%, respectively (16% and 28% higher than the control group); the absorption capacity of neutrophilic granulocytes in the control group was 2.54, and in the first experimental group - 2.67 (5% higher than in the control), in the second experimental group - 3.9 (53% higher than in the control) . The percentage of digestion of neutrophilic granulocytes in the control group was 67%, in the first experimental group - 72% (7% higher), in the second experimental group - 78% (16% higher than the control group).

There was an increase in the content of T-lymphocytes in the first experimental group by 65%, in the second experimental group - by 80%, relative to the control group. The number of B-lymphocytes increased in the first experimental group by 75%, in the second experimental group - by 84%, relative to the control group of animals.

In addition, the drug kargdekhin contributed to an increase in reproductive function, which indicated the timely insemination of animals and a successful outcome of pregnancy, obtaining a healthy offspring.

Therefore, from the results of the studies, we can conclude that the drugs tactivin and cargdechin had a stimulating effect on the metabolic processes and the immune system of animals, namely the production of red blood cells, hemoglobin, total protein and increased the activity of T and B lymphocytes, which allows us to recommend the use of kargdekhin in animal husbandry for the prevention and treatment of gastrointestinal diseases in calves.

Claims (1)

A method for the prevention and treatment of gastrointestinal diseases in calves, including the use of a mixture of water-alcohol tincture from the herb Echinacea purpurea and rhizome of elecampane, prepared on the basis of 70% ethyl alcohol and an aqueous solution of silver diluted in boiled water, which the animal is given orally for 20- 30 minutes before feeding, characterized in that the calves are given twice daily in the morning and evening for no more than 10 days, at a dose of 0.10-0.12 ml per 1 kg of animal weight, diluted with boiled water in an amount of 130-150 ml 180-220 kg weight w just a water-alcohol tincture, which is prepared as follows: 2 parts of Echinacea purpurea herb, 1 part of elecampane rhizome and 1 part of calendula officinalis flowers are mixed with 70 vol.% ethyl alcohol in a ratio of 1: 5 and insisted for 5-9 days at a temperature 36-44 ° C, and the resulting tincture is adjusted to 30% concentration with distilled water, and an aqueous silver solution is introduced into the tincture in a dose of not more than 200 ml, which is not more than 8 mg of silver ions with 100-120 ml of boiled water for 30-40 kg of animal weight.