RU2554498C2 - Composition for prevention of metabolic disorders, increased growth activity in piglets - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: composition comprises succinic acid, trace elements in the form of sulphates of iron, copper, cobalt, zinc and additionally comprises methionine and beet-root molasses at the following content of components in 1000 ml of an aqueous solution: succinic acid - 5.0 g, beet-root molasses - 150.0 ml, methionine - 2.0 g, ferrous sulphate - 10.0 g, copper sulphate - 0.1 g, cobalt sulphate - 0.5 g, zinc sulphate - 0.5 g.

EFFECT: use of the claimed composition has a positive effect on the immune-metabolic status and growth activity of piglets.

3 tbl, 2 ex

Description

The invention relates to veterinary medicine and can be used in animal husbandry to stimulate metabolic processes, growth activity, prevention of hypomicroelementoses and gastrointestinal diseases, to increase the overall resistance of the organism of piglets.

For the prevention of pathophysiological conditions and diseases in newborn piglets, various stimulants of metabolic and immune processes, chemo and antibiotic therapy are widely practiced. In the vast majority of these funds are used as single drugs. The narrow focus of these funds is not effective enough when applied in the system of ensuring the health and safety of newborn piglets.

Currently, in medicine and veterinary medicine, complex drugs of metabolic action based on succinic acid are widely used. The properties of succinic acid and its derivatives have a positive effect even at absolutely low dosages, and enhance the effect of other drugs make it very valuable in the development of complex preparations and biologically active additives (BAA).

The well-known drug "Purivetin" (RF Patent No. 2138271, 09/27/1999). The composition of the drug "Purivetin" includes the following components: weight%: - Riboxinum - 10.0-40.0; calcium glycerophosphate 30.0-40.0; vitamin B 12 - 0.02-0.04; ascorbic acid 2-4; citric acid - 2; succinic acid 2-4; folic acid 1-2, fructose 5-10, glucose - up to 100.

The drug significantly improves metabolic processes, increases resistance to infectious diseases. However, the absence of trace elements in the preparation reduces its effectiveness.

The known composition of a biologically active additive (BAA) (RF Patent No. 2086149 of 08/10/1997): ascorbic acid is additionally introduced into the feed additive containing carboxylic acid salts, and succinic and citric iron and copper salts are added as carboxylic acid salts in the following ratio components, wt.%: succinic iron 30-38, succinic copper 32-40, citric iron 3-7, citric copper 3-7, ascorbic acid 16-20. However, these known compositions do not have a complex immunometabolic effect with the manifestation of an energy-stimulating effect.

The objective of the present invention was to obtain a comprehensive biologically active additives for the prevention and treatment of metabolic disorders, increase growth activity in piglets of the early postnatal period.

An important distinguishing feature of the invention is the inclusion of molasses (molasses), which is a secondary product in the production of sugar from beets, and the essential amino acid methionine with sulfate salts of iron, copper, cobalt and zinc.

To solve this problem, a comprehensive energy-stimulating composition with immunometabolic activity is proposed. The main energy component in the composition is molasses and succinic acid. Succinic acid (UC) is a powerful stimulator of energy production in cells, which is especially important in pathophysiological conditions, when the body does not have enough energy to ensure normal vital functions. Succinic acid and its salts have a wide range of effects on various mechanisms of regulation of the metabolic activity of cells. The object of the action of UC are cells and tissues that are in a state of excitement or pathologically altered. The absolute harmlessness of UC and its compounds, which have the ability to have a positive effect at the lowest dosages, make it very attractive when developing a new generation of so-called “smart” drugs (MN Kondrashova, 1996):

- succinic acid enhances cellular respiration tens of times, which improves the absorption of oxygen by cells, tissues, organs and, conversely, neutralizes aggressive forms of oxygen;

- succinic acid normalizes the functioning of the nervous system, which is of great importance in combating stress;

- succinic acid inhibits inflammatory processes and neutralizes toxins.

The stimulating effect of succinic acid is especially pronounced when weakening the body and its disease.

The physical basis of the proposed composition is beet molasses, which is widely used in industrial animal husbandry as a source of carbohydrates and is a fairly voluminous by-product in the production of sugar.

To enhance metabolic and hepatoprotective activity, methionine is introduced into the composition. Methionine is an essential amino acid and is a “critical” amino acid for newborn animals, because their digestive system cannot synthesize it from nitrogen-containing feed substances. With a lack of methionine, fatty and toxic liver dystrophy is often observed. In the body, methionine activates the action of a number of enzymes, hormones, vitamin B ₁₂ , folic and ascorbic acids. Biochemical processes of neutralization of toxins in the liver occur with the participation of methionine.

For the prevention and treatment of hypomicroelementoses, the composition of the drug includes salts of macro- and microelements: iron sulfate, copper sulfate, cobalt sulfate, zinc sulfate.

Iron sulfate. Iron is involved in hematopoiesis, is part of hemoglobin, and increases the overall resistance of the body. Iron sulfate is prescribed to improve digestion.

Copper sulfate. Copper, like iron, is involved in the processes of hematopoiesis, osteogenesis, is part of the enzyme. Copper accelerates the transition of iron from inorganic forms to organic. This favorably affects the processes of hematopoiesis, especially with alimentary anemia.

Cobalt sulfate. Cobalt affects the hematopoietic functions of the bone marrow, increases the level of hemoglobin and the number of red blood cells, increases the overall resistance.

Zinc sulfate. Zinc stimulates hematopoiesis, the exchange of nucleic acids, proteins, carbohydrates, the growth and development of animals.

The activity of the complex of trace elements and amino acids is enhanced by the action of succinic acid. Succinic acid, as a regulator of metabolic processes, reduces the risk of an overdose of any trace element.

The proposed compositions allow you to get both metabolic and energy-stimulating effects.

Getting the composition.

Example 1

Samples containing 5.0 g of succinic acid, sulfate salts: iron - 10.0 g; copper - 0.1 g; zinc - 0.5 g and cobalt 0.5 g; methionine - 2.0 g, successively dissolved in a volume of 100.0 ml of distilled water when heated. After that, the contents of the flask are poured into a container with 1: 5 molasses diluted with water, respectively 150 ml of molasses and 750 ml of heated drinking water. The resulting composition of biologically active additives is packaged in 1.0 liter plastic bottles.

Example 2

Samples containing 5.0 g of succinic acid and 2.0 g of methionine are dissolved in a volume of 93.0 ml of distilled water with heating. After that, the contents of the flask are poured into a container with 1: 3 molasses diluted with water, respectively 227 ml of molasses and 680 ml of heated drinking water. The resulting composition of biologically active additives is packaged in 1.0 liter plastic bottles.

The experiment was conducted on three groups of piglets with 10 goals in each group, selected on the basis of analogues. The first group of piglets was a control. The second group of piglets was fed test formulations of dietary supplements through a medication with free access to prescription 1: succinic acid - 5 g, molasses - 150 ml, trace elements: iron - 10.0 g, copper - 0.1 g, cobalt - 0.5 g, zinc - 0.5 g, methionine - 2.0 g in a volume of 50.0 ml of an aqueous solution per day; the third group received dietary supplement according to prescription 2: succinic acid - 5 g, molasses - 227 g, methionine - 2 g, in a volume of 50.0 ml of an aqueous solution per day.

Analysis of the indicators shown in table 1, allows us to note a significant increase in hemoglobin in the second group of piglets by 39% and 31% in the third group compared with the control group of animals. A similar increase trend was established in terms of total protein in the range of 11.1% -11.5%, as well as the gamma-globulin fraction, respectively, by 1.0% -4.0%. At the same time, an increase in indicators of natural resistance was established.

It should be noted that the use of an energy-stimulating additive to piglets in the post-weaning period during the growing period from 35 to 65 days significantly affects their growth activity (Table 2).

Thus, an increase in live weight of piglets when using a biologically active additive in prescription 2 occurred by 31.6% compared to the control group, while in piglets receiving dietary supplement for prescription 1, by 48.6%. Thus, the use of a complex of trace elements with methionine in prescription 1 was the most effective.

It should be noted the positive dynamics of increasing hemoglobin by 12.9% -11.4% and total protein by 9.4% -13.4% in the experimental groups of piglets, while a significant increase in reserve alkalinity and an increase in the level of iron in the blood were found. Indicators of copper and cobalt zinc tended to increase and were within the physiological norm (Table 3).

Thus, the use of biologically active additives (BAA) based on molasses with microelements and methionine in piglets during suckling and post-weaning periods has a positive effect on the immunometabolic status and growth activity of piglets.

Table 1 Hematological blood counts of hypotrophic piglets (n = 10) Indicators 1 - (control) 2 - (experienced) 3 - (experienced) age, days 35 35 35 live weight, kg 5.9 ± 0.53 6.3 ± 0.41 6.0 ± 0.35 erythrocytes, × October ¹² / l 8.7 ± 0.54 9.3 ± 0.35 9.1 ± 0.55 hemoglobin, g / l 100.0 ± 0.31 139.0 ± 0.25 * 131.0 ± 0.29 * white blood cells × 10 ⁹ / l 6.9 ± 0.42 7.7 ± 0.27 * 7.5 ± 0.33 total protein, g / l 65.0 ± 0.31 75.0 ± 0.33 * 71.0 ± 0.51 * α-globulins,% fourteen fifteen fourteen β-globulins,% 16 17 16 γ-globulins,% 17 21 * eighteen* BASK,% 47.0 ± 1.3 56.4 ± 3.8 * 55.6 ± 3.95 * FASK,% 16.6 ± 1.88 20.4 ± 1.75 * 19.5 ± 2.1 * LASK,% 35.7 ± 0.35 37.2 ± 0.26 * 34.8 ± 0.26 Note: * - the difference is significant at P≤0,05

table 2 The influence of the tested compounds on the stimulation of the growth activity of hypotrophic piglets Group A drug Live weight kg Average daily gain (g) At the beginning of the experiment
(35 days) At the end of the experiment
(65 days) 1 to. The control 6.2 ± 2.3 13.8 ± 2.9 253.0 2 - op. By recipe 1 6.1 ± 2.4 17.4 ± 2.7 * 376.0 3 - op. According to the recipe 2 6.5 ± 2.3 16.5 ± 3.1 * 333.0 Note: P≤0.05-0.01

Table 3 The dynamics of hematological and biochemical blood parameters in piglets using dietary supplements Indicators Background data On the 10th day Ha 30 days Hemoglobin, g / l 78.56 ± 2.84 81.37 ± 2.94 82.47 ± 3.25 79.42 ± 3.26 89.68 ± 2.75 * 88.46 ± 2.67 * 76.47 ± 2.21 77.25 ± 2.54 79.47 ± 3.11 Erythrocytes, g / l 4.0 ± 0.2 4,5 ± 0,3 4.4 ± 0.3 3.8 ± 0.2 4.0 ± 0.2 3.9 ± 0.2 3.9 ± 0.7 4.1 ± 0.5 4.3 ± 0.3 Total protein, g / l 52.3 ± 2.2 57.2 ± 3.4 * 59.3 ± 3.7 * 53.7 ± 2.4 57.2 ± 2.6 64.8 ± 3.6 * 50.7 ± 0.39 50.7 ± 0.31 51.7 ± 1.35 Reserve alkalinity, total. CO ₂ mg /% 35.1 ± 1.6 39.4 ± 3.2 * 39.8 ± 2.6 * 35.3 ± 1.7 45.4 ± 1.5 * 55.8 ± 1.9 * 35.7 ± 1.7 36.7 ± 1.3 36.7 ± 0.9 Calcium, mmol / L 1.44 ± 0.10 1.98 ± 0.17 2.05 ± 0.12 1.48 ± 0.18 1.55 ± 0.16 1.59 ± 0.13 1.47 ± 0.97 1.51 ± 1.35 1.41 ± 1.23 Inorganic phosphorus, mmol / l 1.32 ± 0.16 1.36 ± 0.18 1.45 ± 0.14 1.31 ± 0.12 1.33 ± 0.11 1.38 ± 0.12 1.33 ± 0.31 1.34 ± 0.87 1.31 ± 0.51 Iron, mcg /% 280.5 ± 2.77 285.5 ± 2.87 290.5 ± 2.33 * 285.5 ± 1.25 298.5 ± 3.25 385.5 ± 1.25 * 281.1 ± 3.17 254.1 ± 3.17 248.1 ± 3.17 Copper, µmol / L 15.7 ± 2.11 16.7 ± 1.11 16.9 ± 0.11 16.3 ± 1.47 15.3 ± 3.47 17.3 ± 3.47 14.1 ± 0.95 15.1 ± 1.95 14.1 ± 2.95 Cobalt, μmol / L 0.43 ± 1.75 0.47 ± 2.75 0.47 ± 1.79 0.48 ± 1.23 0.44 ± 0.23 0.49 ± 1.27 0.44 ± 2.01 0.43 ± 2.47 0.44 ± 1.44 Zinc, micromol / l 43.3 ± 0.53 42.3 ± 4.53 46.3 ± 1.33 41.6 ± 1.09 42.7 ± 3.09 47.6 ± 2.19 44.3 ± 0.98 45.3 ± 2.98 45.3 ± 1.31 Note: the top line - indicators in calves of the first group; middle row - indicators in calves of the second group; bottom line - indicators in calves of the control group. * - P≤0.05-0.01

Claims (1)

Composition for the prevention of metabolic disturbances, increase growth activity in piglets, containing trace elements and organic acid, characterized in that it contains succinic acid, trace elements in the form of iron, copper, cobalt, zinc sulfates and additionally contains methionine and beet molasses with the following components in 1000 ml of an aqueous solution:
succinic acid 5.0 g Beet molasses 150.0 ml Methionine 2.0 g Iron sulfate 10.0 g Copper sulfate 0.1 g Cobalt sulfate 0.5 g Zinc sulfate 0.5 g