RU2437674C1 - Method of obtaining medication, possessing adaptogenic activity - Google Patents

Abstract

FIELD: medicine, pharmaceutics. ^ SUBSTANCE: invention relates to pharmaceutic industry, in particular to method of obtaining medication, possessing adaptogenic activity. Method obtaining medication, possessing adaptogenic activity, by extraction of milled natural raw material, which contains seeds of elettaria, rhizome of elecampane, rhizome of Zingiber oficinale, wood of Caragana jubata with 40% ethanol at room temperature with the following filtration, under defined conditions. ^ EFFECT: medication, obtained by method described above, has expressed adaptogenic activity. ^ 8 tbl, 2 ex

Description

The invention relates to pharmacy and relates to a method for producing funds from a plant collection with adaptogenic activity.

Known and popular adaptogens of plant and animal origin (tincture of magnolia vine, zamani, aralia, ginseng root, lewsee, rhodiola rosea, eleutherococcus, pantocrine, rantacrine, etc.) have the ability to enter the body into a state of non-specifically increased resistance by regulating the course of stress reactions , increasing the resistance of cells and the body in the damaging effect of harmful factors [K.V. Yaremenko. Teaching N.V. Lazarev about SNPS and adaptogens as the basic theory of preventive medicine. // Psychopharmacology and biological narcology. 2005.V.5. Number 4. S.1086-1092]. Despite the growing arsenal of new adaptogens, the development and implementation of new agents with adaptogenic activity from widespread and affordable plant materials remains an urgent task in practical pharmacy.

In connection with the foregoing, based on the study of literature data on the chemical composition and pharmacological properties of individual plants, a formulation of a plant composition consisting of 4 plants was developed. The problem to which the invention is directed, is to create a medicinal product with adaptogenic activity and representing a 40% alcoholic extract obtained from a crushed plant mixture of the following composition (wt.):

real cardamom seeds 34.0 wood caraganes mane 24.0 Elecampane Rhizomes High 23.0 rhizomes of ginger ordinary 19.0

To prepare the proposed tool 34.0 g of cardamom seeds, 24.0 g of carafes mane, 23.0 g of rhizomes of elecampane high, 19.0 g of rhizomes of ginger crushed to a particle size of 0.5-2.0 mm, mixed, extracted at room at a temperature of 40% ethyl alcohol at a ratio of raw materials: extractant 1:10 for 72 hours (3 days), then the extraction is repeated under the same conditions for 24 hours, the extracts are filtered, separated for purification from ballast substances.

The experimental data presented in the text allow us to conclude that the proposed product meets the criterion of "significant differences", because a similar composition was not found in available literature, and the composition and ratio of the ingredients of the product proposed in the claims make it possible to obtain a product with a constant composition of biologically active substances (BAS) with adaptogenic activity. The technical result obtained by carrying out the invention is expressed in expanding the range of highly effective and harmless drugs to increase the body's resistance with increased physical and mental stress.

To prove the materiality of the proposed features of the method, namely, the concentration of the extractant, the ratio of raw materials: extractant, extraction time, a series of experiments were carried out, the data of which are presented below.

For the experiments used raw materials, the quality of which corresponds to the current articles of the Global Fund XI ed. [State Pharmacopoeia of the USSR: Issue 1. General methods of analysis. Ministry of Health of the USSR. - 11th ed., Ext. - M.: Medicine. - 1987. - 337 p .; Issue 2. General methods of analysis. Medicinal plant material. Ministry of Health of the USSR. - 11th ed., Ext. - M.: Medicine. - 1990. - 400 p.] And the corresponding GOSTs [GOST 29046-91. Spices. Ginger. TU; GOST 29052-91. Spices. Cardamom. TU]. The raw materials were crushed separately, in accordance with the requirements of the relevant GOSTs and GF X ed. [State Pharmacopoeia of the USSR. X ed. - M., 1968. Article 349 "Infusions and decoctions." S.370-372]: rhizomes of elecampane high and wood of caragana mane - up to a particle size of 0.5-2.0 mm; ginger rhizomes - up to 0.5 mm, cardamom seeds - up to 0.5 mm. Ready-made dry powders of food spices were used as raw materials: ground ginger, ground cardamom (manufacturer of Novosibirsk Food Plant LLC), fresh ginger rhizomes. The quantitative determination of solids, active substances in the extract was carried out according to the methods described in the literature: polyphenols (tannins), water-soluble polysaccharides according to the methods of the Global Fund XI edition .; ascorbic acid - photocolorimetric method [E.A. Pristupa, D.M. Popov. Improving the technology of preparation and quality control of vitamin teas. / Actual problems of pharmaceutical technology: Scientific. works of VNIIF. - T.32. - M., 1997. - S.151-159]. The active substances of the present cardamom seeds [Formazyuk V.I. Encyclopedia of edible medicinal plants: Cultivated and wild plants in practical medicine. - K .: Publishing house A.S.K., 2003. - 792 p.], Rhizomes of common ginger [Badreldin H. Ali, G. Blunden, M.O. Tanira, A. Nemmar. Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): A review of recent research. // Food and Chemical Toxicology. 2008. V. 43. P.409-420], rhizomes of elecampane high [Belyakov K.V. The use of physico-chemical methods of analysis in the quality control and standardization of raw elecampane high, coltsfoot, common dandelion. // Abstract. diss ... cand. farm.science. - M. 1999. - 23 p .; Konishi T., Shimada Y., Nagao T., Okabe N., Konoshima T. Antiproliferative sesquiterpene lactones from the roots of Inula helenium. // Biol. Pharm. Bull. 2002. V.25. No 10. P.1370-1372] are essential oils. When determining extractives, essential oils volatilize during the drying of an analytical sample. Since the quantitative content of essential oils in alcoholic extracts is difficult to evaluate, the yield of biologically active substances was controlled by the content of polyphenols in terms of tannin. The results of experiments on the selection of extraction parameters are given in table 1.

Table 1 Selection of extraction parameters of the initial plant mixture Test parameter The yield of extractives,% The yield of polyphenols,% 1. The concentration of extractant 20% ethanol 15.26 2,32 40% ethanol 14.54 2.63 50% ethanol 14.86 2.60 60% ethanol 13.34 2.54 70% ethanol 13.15 2.01 80% ethanol 12.06 1.80 96% ethanol 9.35 1.55 2. The ratio of raw materials: extractant 1: 8 11.50 2.05 1:10 14.42 2.63 1:12 14.65 2.69 1:15 16.14 2.74 1:20 18.47 2.98 1:25 21.02 3.15 3. The time and frequency of insisting I phase contact, setting time, h 24 14.42 2,56 48 15.37 2.63 72 18.23 2.83 96 17.28 2.88 II phase contact 24 13.54 0.91 48 13.32 0.92 72 13.45 0.88 96 13.65 0.85 III phase contact 24 5.79 0.24 48 5.85 0.24 72 5.80 0.27 IV phase contact 24 3,7 0.09 48 3.3 0.12 72 3,5 0.08

When the plant mixture is extracted with 40-50% ethanol, a sufficient amount of extractive and polyphenolic substances is extracted, and 40% ethanol is selected as the optimal extractant to obtain the adaptogenic agent. With an increase in the volume of extractant by 50% (ratio of raw materials: extractant 1:15), the yield of extractives increases by only 11.9%, polyphenols - by 4.1%, which indicates the inappropriateness of increasing the ratio of raw materials: extractant above 1:10. When the adaptogenic agent is infused for 72 hours (I extraction) with a ratio of raw materials: extractant 1:10, an equilibrium concentration of extractives and polyphenols is achieved. In the second extraction, the equilibrium concentration is reached within 24 hours. When the raw materials are extracted twice during 72 hours and 24 hours, the yield of extractives and polyphenols is 77% and 91% of the initial content in the raw materials, respectively. In the third and fourth extraction, the yield of polyphenols is 5.8% and 2%, respectively. Thus, the most optimal conditions for obtaining an alcohol extract from the initial mixture are 40% ethyl alcohol, the ratio of raw materials: extractant is 1:10, the first extraction time is 72 hours, the second is 24 hours.

Example 1. Crushed to a particle size of 0.5-2.0 mm and sifted wood, caragana mane, freed from bark - 24.0 g; rhizomes of elecampane high - 23.0 g; mixed with ground cardamom powder - 34.0 g; ground ginger powder - 19.0 g, extracted with 1000 ml of 40% ethyl alcohol at room temperature for 72 hours with periodic stirring, the extraction is filtered into the collector through a dense layer of cloth. 800-850 ml of extraction is obtained, 40% ethyl alcohol in the same volume is added to the meal, it is insisted with periodic stirring for 24 hours, the extraction is filtered in a collection box. The resulting discharge in a volume of 750-800 ml is combined with the first discharge, cleaned from vegetable dust by separation and left to stand for 2 days in the cold (temperature not higher than 10-12 ° C) to remove ballast substances that precipitate during long-term storage. Get 1550-1650 ml of 40% alcohol extract. The solids content of the obtained extract was 18.00 ± 1.50 mg / ml.

The content of biologically active substances in the initial plant mixture, an extract of water-alcohol, meal is shown in table 2.

table 2 The content of biologically active substances in the initial plant mixture, extract, water-alcohol, meal Name The content of biologically active substances (in%) in the original mixture in tincture in meal Polyphenols, calculated on tannin 1.56 ± 0.07 2.63 ± 0.17 0.47 ± 0.03 Vitamin C 5.95 ± 0.15 16.77 ± 0.03 0.70 ± 0.01 Water Soluble Polysaccharides 22.5 ± 1.90 - 19.5 ± 1.22 Note: A dash means that no substances were detected.

To assess the adaptogenic activity of the test substance, we studied the effect of the test substance on the total physical, strength, speed endurance of experimental animals in various models. Previously, before experiments, in order to exclude the influence of ethyl alcohol, the test substance and the reference preparation — liquid eleutherococcus extract (VFS 42-1281-82) were dealcoholized on a rotary evaporator at 37 ° C to 1/10 of the initial volume, the resulting aqueous residue was adjusted with distilled water to the original volume. Statistical processing of the obtained data was carried out by the generally accepted method using the Mann-Whitney criterion [Sergienko VI, Bondareva IB mathematical statistics in clinical studies. M., 2000. - 236 p.]. Differences were considered significant with a probability of 95% (P <0.05).

Determination of acute toxicity of an adaptogenic agent. The experiments were carried out on mice of the CBA line of both sexes weighing 18-20 g. The acute toxicity of the adaptogenic agent was determined using the Kerber method [Belenky M.L. Elements of a quantitative assessment of the pharmacological effect. - L., 1963. - 148 p.] With its single intragastric and intraperitoneal administration in the form of a dealcoholized aqueous solution in doses from 1.0 to 395.0 ml / kg of animal weight. The final volume administered to the animals was 1.0 ml / kg. Animals of the control groups received an equivolume amount of distilled water. Observation of the animals was carried out for 14 days from the date of administration of the test drug. Throughout the entire period of the experiment, the general condition and behavior of animals was observed, visible signs of intoxication and the number of dead animals were recorded with examination of internal organs and histological examination of vital organs during the death of animals.

It was found that intragastric and intraperitoneal administration of an adaptogenic agent in volumes of 1.0-395 ml / kg did not cause the death of animals during the entire observation period. In animals treated with the test drug, there were no visible signs of intoxication; behavioral reactions and general condition did not differ from that in animals of the control group; the animals of the experimental groups remained active, took good food, and the stool was normal during the entire observation period. The data obtained allow us to attribute the adaptogenic agent to the group of practically non-toxic substances according to the classification of K. Sidorov. [Sidorov K.K. On the classification of toxicity of poisons with parenteral routes of administration. // Toxicology of new industrial chemicals. - M., 1973. S. 47-51].

Studying the influence of adaptogenic agents on the general physical endurance of experimental animals.

The experiments were carried out on CBA mice of both sexes weighing 19 - 20 g. To determine the total physical endurance, we used the generally accepted method of swimming in a pool with a water temperature of 20.0 ± 0.5 ° C with a load of 5% of body weight in accordance with the methodological recommendations set out in the manual [Determining the safety and effectiveness of dietary supplements. Methodical instructions. - M., 1999. - 86 p.]. In order to determine the dose-dependent effect, a dealcoholized solution of the test agent was administered intragastrically once 1 hour before testing in volumes of 0.5; 1.0; 3.0; 5.0 and 7.0 ml / kg. When studying the time-dependent effect, the drug was administered prophylactically once in a volume of 3.0 ml / kg per 0.5; 1.0; 3.0; 6.0 and 24.0 hours before testing. To study the dependence on the frequency of administration, the test agent was administered prophylactically in volumes of 1.0; 3.0 and 5.0 ml / kg for 3, 7 and 14 days 1 time per day 30 minutes before feeding. Animals of the control group received an equivolume amount of distilled water. Hereafter, a dealcoholized solution of the extract of Eleutherococcus in volumes of 3.0 and 5.0 ml / kg was used as a comparison drug. The total physical endurance of animals was determined by swimming the animals to complete fatigue, the criterion of which was a 10-second immersion of the animal under water. The data obtained are given in table 3-5.

As follows from the data given in table 3, a single administration of the test drug to white mice in volumes of 0.5 and 1.0 ml / kg did not significantly affect the duration of swimming of animals, whereas when using the tool in a volume of 3.0 ml / kg d there was an increase in overall physical endurance by 32% compared with data in mice of the control group. With an increase in the volume of the test drug, an increase in its adaptogenic activity was noted. So, when introduced in a volume of 5.0 ml / kg, the swimming time of animals of the experimental group increased by an average of 45% compared with that of mice of the control group. A single administration of the drug in higher doses (5.0 and 7.0 ml / kg) revealed a similar adaptogenic effect: physical performance increased by an average of 40-45% compared with the data of animals in the control group. At the same time, the effectiveness of the test drug was similar to that of the comparison drug - Eleutherococcus extract in similar doses. Given that the swimming time of animals receiving the test drug in a volume of 3.0 ml / kg was not statistically different from that in mice receiving it in volumes of 5.0 and 7.0 ml / kg, in further experiments, as experimental the therapeutic volume of the test agent corresponding to 3.0 ml / kg was used.

Table 3 The effect of adaptogenic agents on the overall physical endurance of white mice, depending on the volume administered Groups of animals Volume ml / kg n Duration of swimming, min Control (H ₂ O) - 12 14.6 ± 1.32 Experimental 1 (test tool) 0.5 fifteen 14.8 ± 1.35 Experienced 2 (test tool) 1,0 fifteen 15.5 ± 1.40 Experienced 3 (test tool) 3.0 fifteen 19.3 ± 1.70 ^∗ Experimental 4 (test tool) 5,0 fifteen 22.2 ± 1.48 ^∗ Experimental 5 (test tool) 7.0 12 20.5 ± 2.08 ^∗ Experienced 6 (Eleutherococcus) 3.0 12 19.8 ± 1.23 ^∗ Experienced 7 (Eleutherococcus) 5,0 8 20.4 ± 2.34 ^∗ Note: ^∗ - hereinafter, values significantly different from the data of the control group at P <0.05

It was also established that the severity of the adaptogenic effect of the test drug depends on the time of administration (Table 4).

Table 4 The effect of adaptogenic agent in a volume of 3.0 ml / kg on the total physical endurance of white mice depending on the time of administration Groups of animals Time after administration, h n Duration of swimming, min Control (H ₂ O) - twenty 15.2 ± 1.38 Experimental 1 (test tool) 0.5 fifteen 18.9 ± 1.50 Experienced 2 (test tool) 1,0 fifteen 21.4 ± 1.46 ^∗ Experienced 3 (test tool) 3.0 fifteen 20.3 ± 2.05 ^∗ Experimental 4 (test tool) 6.0 fifteen 19.2 ± 1.23 ^∗ Experimental 5 (test tool) 24.0 fifteen 15.7 ± 1.20

From the data given in the table, it follows that the stimulating effect of the test drug is manifested already 30 minutes after its single administration in the volume of 3.0 ml / kg: the duration of swimming of white mice increased by 25% compared with the control (the difference is not significant) . In 1.0 hour after drug administration, the efficiency of white mice increased by almost 40% and remained at that level for another 3 hours, after which the adaptogenic effect began to decrease: after 6 hours, the swimming time of the mice of the experimental group exceeded the control data only by 24%, and after a day from the moment of drug administration, the working capacity of animals of the experimental group decreased to the level of mice in the control.

When studying the dependence of the pharmacological effect of the drug on the frequency of its administration, it was found that repeated prophylactic administration to white mice is accompanied by an increase in adaptogenic activity (Table 5).

Table 5 The effect of adaptogenic agents on the overall physical endurance of white mice, depending on the frequency of administration Groups of animals Volume ml / kg n Duration of swimming, min with 3-day administration with 7-day administration with a 14-day administration Control (H ₂ O) - 12 16.4 ± 1.53 17.5 ± 1.55 17.2 ± 1.42 Experimental 1 (test tool) 1,0 fifteen 23.4 ± 2.30 ^∗ 25.6 ± 1.33 ^∗ 28.3 ± 2.15 ^∗ Experienced 2 (test tool) 3.0 fifteen 28.0 ± 2.11 ^∗ 33.8 ± 2.40 ^∗ 29.4 ± 1.46 ^∗ Experienced 3 (test tool) 5,0 fifteen 29.3 ± 2.52 ^∗ 30.5 ± 3.02 ^∗ 31.2 ± 2.58 ^∗ Experienced 4 (Eleutherococcus) 3.0 12 28.6 ± 1.84 ^∗ 28.5 ± 1.85 ^∗ 27.6 ± 2.35 ^∗

As follows from the table, the most pronounced increase in physical endurance is observed when it is administered for 7 days in volumes of 3.0 and 5.0 ml / kg: the swimming time of mice of the indicated experimental groups increases by 93% and 74%, respectively, compared with animal data control group. The drug exerted a slightly less pronounced effect when administered within 3 days, and when administered within 14 days, its effectiveness was similar to that when administered within 7 days. At the same time, the adaptogenic activity of the test agent with repeated administration was superior to that of the comparison drug - Eleutherococcus extract.

Thus, the data obtained indicate that the test tool has a stimulating effect on overall physical endurance, characterized by the predominance of aerobic processes of tissue energy supply. It was found that with a single injection, the drug has the most pronounced adaptogenic effect in volumes of 3.0-5.0 ml / kg when administered 1.0 hour before the study; its effectiveness increases with repeated prophylactic administration: the most pronounced increase in physical endurance is observed with its 7-day prophylactic administration in a volume of 3.0 ml / kg.

Example 2. Crushed to a particle size of 0.5-2.0 mm and sifted wood caraganes mane - 24.0 g; rhizomes of elecampane high - 23.0 g; cardamom seeds - 34.0 g; fresh ginger rhizomes with a moisture content of 84%, cut to a particle size of 0.5 mm - 35.0 g, are mixed, extracted with 1000 ml of 40% ethyl alcohol at room temperature for 72 hours with periodic stirring, the extraction is filtered into the collector through a dense layer of cloth . 880 ml of extraction is obtained, ethyl alcohol is poured into the meal in the same volume, it is insisted with periodic stirring for 24 hours, the extraction is filtered into a collection box. The resulting discharge in a volume of 850 ml is combined with the first discharge, cleaned from vegetable dust by separation and left to stand for 2 days in the cold (temperature not higher than 10-12 ° C) to remove ballast substances that precipitate during long-term storage. Obtain 1730 ml of 40% alcohol extract. The solids content of the obtained aqueous-alcoholic extract was 14.54 ± 1.20 mg / ml.

Study of the effect of adaptogenic agents on the speed endurance of experimental animals.

The experiments were carried out on mice of a CBA line of both sexes weighing 18-20 g. The effect of the test substance on high-speed physical endurance was determined by the duration of the animals running on a 10-track treadmill at a treadmill blade speed of 40 m / min [Determination of the safety and effectiveness of biologically active food additives . Methodical instructions. - M., 1999. - 86 p.]. The test agent was administered intragastrically in a volume of 3.0 ml / kg once 1 hour before testing, as well as prophylactically, repeatedly, for 7 days in the indicated volume 1 time per day 30 minutes before feeding. The animals of the control group were intragastrically injected with a similar volume of distilled water. As a comparison drug, a dealcoholized solution of Eleutherococcus extract was used in a volume of 3.0 ml / kg. High-speed physical endurance was evaluated by the duration of the run of the animals until complete fatigue, the criterion of which was the 10-second stay of the animal on the treadmill electric grid under a voltage of 70 V. The data obtained are shown in table 6.

Table 6 The effect of adaptogenic agents on the speed physical endurance of white mice Groups of animals n Running Duration, s with a single administration with 7-day administration Control (H ₂ O) 10 210.3 ± 15.6 218.5 ± 23.6 Experimental 1 (test tool) 12 226.5 ± 19.3 296.0 ± 29.4 ^∗ Experienced 2 (Eleutherococcus) 12 218.0 ± 23.5 268.3 ± 25.5 ^∗

As follows from the data given in Table 6, a single administration of this agent in the indicated volume, as well as the comparison drug, did not significantly affect the speed physical endurance of white mice, the running time of the animals of the experimental group did not statistically differ from that of the animals of the control group (p ≥0.05).

It was found that repeated prophylactic administration of the test drug for 7 days was accompanied by a significant increase in speed endurance, as evidenced by an increase in the running time of animals of the experimental group by 35% compared with the data of mice in the control group. At the same time, the effectiveness of the test agent exceeded that of the extract of Eleutherococcus. Thus, the obtained data indicate that the test drug in the volume of 3.0 ml / kg increases the speed physical endurance, characterized by the predominance of anaerobic processes in the tissues only with repeated administration.

The effect of adaptogenic agent on speed endurance against the background of dosed physical activity was studied. The experiments were carried out on mice of the CBA line of both sexes weighing 18-20 g. Preliminary dosed physical activity was carried out using forced run of animals in a treadmill at a belt speed of 28 m / min for 5 minutes. Animals that received preliminary physical activity were divided into 2 subgroups. In the first subgroup, the mice were subjected to a single physical activity for 5 minutes. After this, the test substance was intragastrically administered to the animals in a volume of 3.0 ml / kg, and after 1 hour, fast physical endurance was determined using the method described in the previous example. Animals of the second subgroup received daily dosed physical activity for 7 days. On the first day, the duration of the run was 5 minutes; on the following days, the duration of the load was sequentially increased by 1 minute. Immediately after the load, the test substance was administered to the mice in a volume of 3.0 ml / kg. The animals in the control group were injected with distilled water in a similar volume. On the 7th day, 1 h after the last injection of the indicated agent, speed endurance was determined by the duration of the run in the treadmill until fatigue. The data obtained are given in table.7.

Table 7 The effect of adaptogenic agents on the speed endurance of white mice on the background of preliminary physical exertion Groups of animals Running Duration, s at single load at 7 days load Intact (n = 8) 248.5 ± 23.4 ^∗ 248.5 ± 23.4 Control (H ₂ O), (n = 15) 153.3 ± 14.5 294.0 ± 26.5 Experienced (test agent), (n = 15) 235.7 ± 22.6 ^∗ 496.0 ± 28.2 ^∗

The data shown in table 7 indicate that a single dosed load causes a decrease in performance in mice of the control group, as indicated by a significant decrease in the duration of running of animals of this group - an average of 40% compared with data from intact mice). A single injection of the indicated agent in a volume of 3.0 ml / kg against a background of a single dosage load had a protective effect, preventing a decrease in the physical performance of the mice of the experimental group. So, in mice that received the test drug, the duration of running did not statistically differ from that in intact animals.

In the second series of experiments, it was found that in animals of the control group subjected to preliminary (7-day) dosed physical activity, there is a tendency to increase the duration of running in the treadmill (the difference is not statistically significant). Moreover, the daily administration of the test drug against the background of dosed loads had a pronounced stimulating effect on the speed physical endurance of the mice of the experimental group, as evidenced by an increase in the running time of mice of the experimental group by an average of 70% compared with the data of animals in the control group (p≤0.01) .

The data obtained indicate that the introduction of the test substance in the indicated volume against a background of dosed physical exertion has an adaptogenic effect, preventing the development of fatigue after a single load and maintaining the physical performance of mice at the level of indicators of intact animals. The test agent exerts a more pronounced adaptogenic effect upon repeated administration against the background of daily dosed physical activity.

The study of the influence of the test tool on the endurance of experimental animals.

The experiments were carried out on mice of the CBA line of both sexes weighing 18-20 g. In the experiments, the model “hanging on a pole” was used. The animals of the experimental group were intragastrically injected with a dealcoholized solution of the indicated agent in a volume of 3.0 ml / kg once for 1 hour before testing, and also repeatedly for 7 days in the indicated volume, 1 time per day 30 minutes before feeding. The animals of the control group were intragastrically injected with a similar volume of distilled water. The effect of the test substance on endurance was determined by the time "animal hanging on a pole" from the beginning of retention to the time of fall. The experimental data are given in table.8.

Table 8 The effect of adaptogenic agents on the strength endurance of white mice Groups of animals Duration of hanging on a pole, min with a single administration with 7-day administration Control (H ₂ O, n = 10) 12.5 ± 1.42 13.0 ± 1.62 Experimental 1 (test tool, n = 15) 16.3 ± 1.35 ^∗ 20.4 ± 1.45 ^∗ Experienced 2 (Eleutherococcus, n = 12) 16.5 ± 1.32 ^∗ 19.4 ± 1.06 ^∗

As follows from the data given in table 8, with a single administration of this agent in a volume of 3.0 ml / kg, the hanging time of mice of the experimental group increases by 30% compared to that in animals of the control group. Repeated administration of the test drug is accompanied by a more pronounced increase in the animal's endurance of strength, as evidenced by an increase in the duration of the hanging mice of the experimental group by an average of 60% compared with the data in animals of the control group. The effectiveness of the test drug was similar to that of the comparison drug.

Thus, the data obtained indicate that the test agent has adaptogenic activity, while the most pronounced activity was manifested with repeated prophylactic administration in a volume of 3.0 ml / kg against a background of daily dosed physical activity.

Claims (1)

The method of obtaining funds with adaptogenic activity by extracting plant materials containing cardamom seeds of the present, elecampane root rhizome, ordinary ginger rhizome, crushed to a particle size of 0.5-2.0 mm, 40% ethyl alcohol at room temperature, followed by by filtering, characterized in that the feedstock additionally contains wood of carafes of mane, extraction is carried out twice for 72 hours and 24 hours at a ratio of feed: extractant 1:10, the extracts are combined, while the feed is taken into parts by weight:
real cardamom seeds 34.0 wood caraganes mane 24.0 Elecampane Rhizomes High 23.0 rhizomes of ginger ordinary 19.0