SU1745256A1 - Method for preparation of remedy, showing adaptogenic activity - Google Patents

Abstract

The invention relates to medicine, namely to pharmacy, and concerns a method of polt a student from a mixture of vegetable raw materials with adaptogenic activity. The goal is to increase the yield of extractive substances. The method consists in extracting a 35-45% ethanol solution of the dried ground mixture of the following plant composition, parts by weight: roots and rhizomes of virgins high 2.3-3-3.0; Fruits Blony fit 1.8-2.5; cardamom fruits 1.8-2.5; fruits bo ryshnika 1.8-2.5; ginger roots 2.3-2.8; wild rose 1.8-2.5; grass tim on creeping 1.8-2.5; fruits of juniper ordinary 0.8-1.5; licorice roots 1.8-2,5. The method allows to increase the yield of extractive substances up to 20-22%. 5 tab.

Description

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The invention relates to the field of pharmacy and relates to a method for the preparation of an agent from plant raw materials having adaptogenic activity.

A known method of producing an agent with adaptogenic activity, including extraction of the roots of Eleutherococcus with a solution of ethanol.

The disadvantage of this method is the low yield of extractives, as well as the limited resources of this raw material and the high cost associated with it. The yield of extractives is 6%.

The aim of the proposed method is to increase the yield of extractives.

The goal is achieved by the fact that the method involves the extraction with 35-45% ethanol solution of the dried ground mixture of the following plant composition, wt.h .;

Dev forces high f roots and rhizomes) 2.3-3.0 Apple trees fit

(fruits) 1.8-2.5

Cardamom

(fruits) 1.8-2.5

Bo ryshnik

(fruits) 1.8-2.5

Ginger

(roots) 2.3-2.8

Rosehip

(fruits) 1.8-2.5

Tim n creeping (grass) 1.3-2.0

2

Sya n) sya os

Juniper

ordinary

(fruits) 0.8-1.5

Licorice Goals

(roots) 1.8-2.5

Examples of specific implementation method.

Example 1. Preparation of the agent at lower boundary values of the ratio of ingredients of the raw materials. Crushed (sieve No. 30J roots and rhizomes of virgins strength high 230 g, roots of ginger 230 g, licorice roots 180 g crushed (sieve No. 05) blonds of 180 g, cardamom fruits 180 g, boys of 180 g, rosehips 180 g, juniper fruit 80 g, crushed (Ms 50 sieve), tim grass per creeping 130 g mixed and then loaded into a percolator, filled with 43.75 l of a 35% ethanol solution in the ratio of raw material: extractant 1:25 taking into account the water absorption coefficient. Extraction is carried out at room temperature with occasional stirring for 96 hours. Removed The mixture is drained, allowed to stand and filtered and the volume drained is 39.370 l. The clear light brown liquid has a density of 0.956 and the percentage of ethanol is 34.7%. The dry residue is 21.3%.

PRI mme R 2. Preparation of the agent at average values of the ratio of ingredients of the raw materials. Shredded (sieve No. 30) roots and rhizomes of virgins high 250 g, roots of ginger 250 g, licorice roots 200 g, crushed (sieve No. 05) blonds 200 g, fruits of cardamom 200 g, fruits of boyster 200 g, hips. 200 g, juniper fruits 100 g, chopped grass (sieve No. 50), thyme per creeping 150 g are mixed and then loaded into a percolator, 52.500 l of 40% ethanol solution is poured at a ratio of raw materials: extragent 1:30 taking into account the water absorption coefficient . Extraction is carried out at room temperature with occasional stirring for 96 hours. The extracts are discarded, allowed to stand and filtered. The volume of the drain is equal to 48,125 l. A clear, light brown liquid is obtained. Density 0,9484. The percentage of ethanol is 39.78%. The dry residue is 23.56%.

Example 3: Preparation of the agent at the upper limit values of the ratio of ingredients of the raw materials. Crushed (sieve No. 30) roots and rhizomes of virgins high power 300 g, ginger roots 280 g, licorice roots 250 g, crushed (sieve No. 05) blonds 250 g, cardamom fruits 250 g, boys of 250 g, hips. 250 g, juniper fruit 150 g, crushed (sieve number 50) grass tim on creeping

200 g are mixed and then loaded into the percolator, 61.25 l of a 45% ethanol solution is poured in the ratio of raw materials: extragent 1:35 taking into account the absorption coefficient

Extraction is carried out at room temperature with occasional stirring for 96 hours. The extracts are discarded, allowed to stand and filtered. The volume of the drain is 56.88 dPuy transparent liquid is light brown. The density is 0.9396, the percentage is 44.98%, the dry residue is 20.8%.

Pharmacological studies of the means obtained by the proposed method; carried out in accordance with generally accepted requirements. Experiments were carried out on white male rats with an initial mass of 150-160 g, which were intraperitoneally administered once.

0 means in a dose of 0.01 g / kg - 0.05 g / kg. Eleutherococcus extract was administered to the second group of rats in isoeffective doses of 0.01 g / kg - 0.05 g / kg in a similar pattern. A third group of animals received an equivalent volume in distilled water. The degree of adaptogenic activity was judged by the duration of the running of animals in a treadmill, the duration of swimming of white mice with a load, the effect of the agent on the immune system was studied in experiments on mice with FI hybrids when administered once a day for 10 days , 01 g / kg. Statistical data processing was performed using the Moldenhauer coefficient.

The results of the dependence of the degree of adaptogenic activity of the obtained agent on the limits of the ratios of the incoming ingredients are given in Table. one.

The results of the effect of the agent with a single injection at a dose of 0.03 g / kg and 0.05 g / kg to rats on the duration of the run in the treadmill are given in Table 2.

In the continuation of pharmacological studies, experiments were carried out with the introduction of the obtained agent once a day at a dose of 0.01 g / kg for 10 days. Another group of rats under similar conditions was administered an extract of Eleutherococcus at a dose of 0.1 g / kg. The control group of rats received an equivalent amount of distilled water. The results of the effect of the agent in a dose of 0.01 g / kg on the duration of the run in the treadmill (after 10 days) are presented in Table. 3. Along with these experiments was

5, an experiment was conducted to evaluate the effect of the obtained agent on the duration of swimming of white mice at a water temperature of + 28 ° C. Animals with a mass of 20 g were injected with a dose of 0.01 g / kg once a day for 10 days. After 10 days, white mice attached the load to the tail - 10% of the total mass. Another group of mice, instead of the agent, were administered the extract of Eleutherococcus according to the same scheme, and the control group of animals received an equivalent amount of distilled water.

The results of the funds obtained for the duration of swimming of white mice are given in Table. four.

In addition, the nature of the effect of the obtained agent on the body’s immune system was carried out. Experiments were carried out on FI hybrids with an initial weight of 20 g, which were administered intragastrically once a dose of 0.01 g / kg for 10 days. After 10 days, animals were immunized with sheep erythrocytes (EB), and on the fourth day thereafter, the number of antibody-forming cells (KLA) was determined according to the Cunningham method. A mixture of a suspension of spleen cells, sheep erythrocytes and complement in equal volumes was introduced into a chamber made of glass slides and incubated for one hour at a temperature of + 37 ° C. Then, the number of plaques in the chamber (the number of AFC) was counted. To calculate the number of AFC per number of spleen cells, they counted the number of the latter in the Gorev chamber.

The results of the influence of the obtained agent on the body’s immune system are given in Table. five.

The tool increases the number of antibody-forming cells in mice, i.e. stimulates the primary immune response to the introduction of antigen in the form of red blood cells ba5

10 15 0 5

0 -

five

Claims (1)

wound, has a pronounced adaptogenic activity. The degree of specific activity of the agent is not inferior to the extract of Eleutherococcus, and the amount of extractive substances is increased to 20-22%. Claims for obtaining an agent with adaptogenic activity by extracting vegetable raw materials with aqueous ethanol, characterized in that. In order to increase the yield of extractive substances, a mixture of roots and rhizomes of high virgins, fruits of blonds, fruits of cardamom, berries, ginger, rose hips, thyme on creeping, juniper fruits, licorice roots is used as vegetable raw materials. in the following ratio of components, parts by weight: Roots and rhizomes of virgins power high 2.3-3.0 Fruits Blony suitable 1.8-2.5 Fruits of cardamom 1.8-2.5 Fruits of bo ryshnika1,8-2,5 Roots ginger 2,3-2,8 Rose hips1,8-2,5 Grass Tim on creeping 1.3-2.0 Fruits of juniper vulgaris 0.8-1.5 Roots of licorice 1.8-2.5 Extraction is carried out with 35-45% ethanol solution in the ratio of raw materials: extragent 1 25-35 for 96 h at room temperature Table 1 at Conditions of experience The number of animals in the group Control group (with NAO) Experimental group (with means) Experimental group (with extract of Eleutherococcus) Continued tabl, 1 table 2 Table 3 Table 4 Duration of swimming mice, min 23.0 ± 1.5 31.3 ± 1.9 , 05 30.1 ± 2.2, 05 Table 5