RU2462260C2 - Herbal anthelminthic - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention refers to veterinary helminthology and consists in the use of common agrimony Agrimonia eupatoria L. as an agent for "экцистированных" Fasciola hepatica larvae in preventing fascioliasis.

EFFECT: invention provides higher clinical effectiveness of the agent.

3 tbl, 2 ex

Description

The invention relates to veterinary helminthology, relates to funds based on plant materials for the prevention of helminthiasis of farm animals, in particular fascioliasis of cattle.

For the treatment and prevention of helminthiases in farm animals, synthetic anthelmintics are used. Our studies have shown that the effectiveness of known methods depends on the intensity of invasion. With a high intensity of invasion (II - 183 ind. Per animal), about 80% of fasciol in the liver of cattle parasitize for a long time in a state that has not developed to the imaginal stage, and with AI less than 50 ind. per animal in this state are 10-25%. Considering that synthetic anthelmintics are effective mainly against mature forms of fasciol, after treatment, parasites in a state that has not developed to the imaginal stage remain alive and continue to negatively affect the organism of the definitive host. In addition, a number of anthelmintics, in particular phenothiazine, piperazine salts, carbon tetrachloride, arsenic, cadmium, and copper preparations, are capable of causing toxicosis, dysbiosis, immunosuppression, and even death of animals (niclofolone in therapeutic doses), other anthelmintics, in particular, oxfendazole, , cambendazole, parbendazole, carbon tetrachloride have an embryotoxic effect. Even non-toxic anthelmintics can cause poisoning caused by the action of helminth decomposition products absorbed from the intestine. Helminth decomposition products cause general intoxication and reduce immunity. In addition, when using individual anthelmintics, certain restrictions and waiting times must be taken into account. So, when albendazole is administered to inseminated cows at a dose of 25 mg / kg body weight on 7 and 14 days of pregnancy, embryo-lethality increases, praziquatel is not recommended for lactating cows and young animals under 1 month old, and cambendazole for dairy cattle and egg-laying birds (increases the number eggs incapable of fertilization) / 1 /. Low-toxic and non-toxic preparations are considered to be preparations based on plant materials, which are widely used in medical practice, for example, hepatoprotective, choleretic and anthelmintic agent “Kumivit”, which is a processed product from the production of pumpkin seed oil / 2 /, antiparasitic tincture of biologically mature walnut pericarp walnut (before the fetus falls out of the pericarp) / 3 /, a medicinal product, including alcohol tincture of walnut, wormwood and cloves / 4 /, angel intoe means comprising a dry powder extract and wormwood herb and Tansy, powder carnation flower buds / 5 /. In veterinary practice, preparations based on plant materials are used for the prevention and treatment of mainly nematodoses and cestodoses (table 1). Pumpkin cake treat helminthiases of farm animals / 6 /. Known plant-based anthelmintics, like synthetic anthelmintics, act primarily on mature parasites.

Table 1 Anthelmintic plants Plant Disease Kind of animal Application Male fern fascioliasis sheeps free group feeding cestodoses dogs
birds individually-compulsory individual or free group feeding Needles ascariasis pigs free group feeding Pumpkin seeds cestodoses dogs, geese free feeding Blueberries cestodoses of nematodoses foxes free feeding Tansy nematodoses horses, dogs individual forced feeding Sagebrush moniesiosis calves, lambs individual forced feeding Ledum makrakantorinhoz pigs group feeding Garlic cestodoses birds free or compulsory Hypericum perforatum nematodoses pigs group feeding Snowdrop nematodoses, cestodoses sheeps free feeding Aspen leaves nematodoses horses free feeding Aft lupine ascariasis pigs, birds free feeding Yarrow nematodoses sheeps free feeding Sorrel nematodoses sheeps free feeding Stinging nettle trematodoses sheeps individually forced The cinquefoil is erect trematodoses sheeps free feeding

The problem to which the invention is directed, is to expand the range of anthelmintic agents based on plant materials by creating an anthelmintic agent that acts on the excised Fasciola hepatica larvae that are at the beginning of invasion in the digestive tract of the definitive host, which are temporarily immobilized or killed in transit are excreted masses.

The problem is solved by using, as an anthelmintic, a powder of dried grass, an ordinary (pharmacy) Agrimonia eupatoria L.

Common agrimony (pharmacy) - Agrimonia eupatoria L. Rosaceous family - a perennial herb. Distributed in the European part of Russia and the Caucasus. It grows in bright forests, at the edges, meadows, pastures and along fields. In medicine, the aerial part of the plant is used. The grass contains carbohydrates: polysaccharides, glucose, fructose, sucrose; fatty and organic acids (citric, malic, tartaric, oxalic, quinic); steroids; nitrogen containing compounds; phenylcarboxylic acids; tannins; catechins; flavonoids; vitamin C; higher aliphatic hydrocarbons and alcohols; wax. It has astringent, choleretic, antibacterial and anti-inflammatory effects. In folk medicine, an infusion of grass is an ordinary (pharmacy) mortar used for diseases of the liver and gall bladder, as a gastric drug and an antidote for poisoning, as well as diarrhea, worms, hemorrhoids, warts, hemoptysis, renal and uterine bleeding, inflammation of the bladder, and nighttime incontinence urine and swelling. As an external remedy, the plant is used in inflammatory processes. In veterinary practice, an ordinary (apothecary) insect repellent is used for lice in horses. Honey plant.

The essence of the method is illustrated by examples.

Example 1. The rabbits of the breed gray giant 4 months of age were divided into two groups: experimental and control 5 animals each. Animals of both groups were infected twice with an interval of 14 days by oral administration of two-month-old F. hepatica adolescari at the rate of 50 ind. per animal. The day before infection, on the day of infection and on the next day, the experimental animals were fed an ordinary (pharmacy) replica in the form of a large powder (dried raw materials were crushed mechanically to a particle size of 1-3 mm), which was mixed with bran (rye or wheat) before use in a ratio of 1: 1, it was poured with warm water (35-40 ° C) until a wet mix was formed. The daily dose is 10 g. Rabbits of the control group and the experimental group received helminth-free cereal hay during the waiting period. The duration of the experiment was 72 days (after the first infection) until fascioli reached maturity, and the growth period of trematodes from the second infection was 58 days. Animals were killed and the liver was examined for the presence of grown helminths from anthelmintic exposed excised F.hepatica larvae.

table 2 The effectiveness of the action of the common reprosy (pharmacy) on excised F. hepatica larvae during experimental infection of rabbits Indicators Experienced group - common repeshka (pharmacy) Control group after the first infection after the second infection in experience Trematode size, cm 1.4-1.5 0.7-1.0 0.7-1.5 0.7-1.5 The intensity of invasion (II), ind. 7 7 fourteen 34 % AI to control 20.58 20.58 41.17 100.0 Intensity Efficiency (IE),% 79.42 79.42 58.83 0

As can be seen from table 2, the common agaric (pharmacy) is effective against excised F.hepatica larvae: the number of helminths per liver in the experiment is 2.43 times less than in the control. In addition, with the repeated use of the preparation of common (pharmaceutical) flaxseed during the period of ingestion of F. hepatica adolescarius into the gastrointestinal tract of rabbits, its effectiveness was maintained, as evidenced by the ratio of the number of grown trematodes after the first and second infections

Example 2. Gobies of black-motley breed of 5-6 months of age, twice examined for the presence of helminths with negative results, were divided into 2 groups of 5 animals each: experimental and control. Animals of both groups were infected twice with an interval of 14 days by oral administration of two-month-old F. hepatica adolescari at the rate of 125 ind. per animal. The day before infection, on the day of infection and the next day, the experimental animals were fed an ordinary (pharmacy) replica in the form of a large powder (dried raw materials were crushed mechanically to a particle size of 1-3 mm), which was mixed with bran (rye or wheat) before use in a ratio of 1: 1, it was poured with warm water (35-40 ° C) until a wet mix was formed. The daily dose is 50 g. The rest of the feed is by the age norm: 50% of the daily diet was made up of concentrates and hay from cereals harvested from land plots. Box contents, feeding conditions and watering excluded the possibility of infection of animals with fascioli. The duration of the experiment is 72 days after the first infection and 58 days after the second infection. Animals were killed and the liver was examined for the presence of fasciol. The results are presented in table 3.

Table 3 Efficiency of the action of the common reprosy (pharmacy) on excised F. hepatica larvae during experimental infection of gobies Indicators Experienced group - common repeshka (pharmacy) Control group after the first infection after the second infection in experience Trematode size, cm 2.0-2.1 0.5-1.4 0.5-2.1 0.5-2.1 The intensity of invasion (II), ind. four four 8 eighteen % AI to control 22.22 22.22 44,44 100.0 Intensity Efficiency (IE),% 77.78 77.78 55.56 0

As can be seen from table 3, the common lichen (pharmacy) leaf is effective against excised F.hepatica larvae: the number of helminths per liver in the experiment is 2.25 times less than in the control. In addition, with repeated use of the drug, ordinary (pharmacy) flare, during the period when the gobies of F. bullia hepatica enter the gastrointestinal tract, its effectiveness was maintained, as evidenced by the ratio of the number of grown trematodes after the first and second infections.

When studying the toxic properties of an ordinary (pharmacy) mortar, it was found that concentrations of SK ₁₀ , SK ₅₀ , SK ₁₀₀ allowed it to be assigned to the group of low toxic substances for mammals, fish and other aquatic organisms, and according to the accumulation coefficient, to drugs that did not have cumulative properties.

Information sources

1.http: //vetmedical.ru.

2. RF patent No. 2280445, 2006.

3. RF patent No. 2182012, 2002.

4. RF patent No. 2132693, 1999.

5. RF patent No. 2241482, 2004.

6. RF patent No. 2241482, 2004.

Claims (1)

The use of common repress (Agrimonia eupatoria L.) as a remedy against excised Fasciola hepatica larvae in the prevention of fascioliasis.