RU2259818C1 - Combined incubated eggs treatment for disinfecting and stimulating embryonal and post-embryonal development in poultry - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: method involves sequentially treating eggs received from laying hens with ozone taken in concentration of 343-350 mg/m³ during 60 min, with succinic acid taken as 0.2-0.3% solution at a dose of 1.5-2 ml/m³ with exposure time in aerosol cloud being not longer than 30-35 min and with para-aminobenzoic acid given at calculated dose of 10^-22-10^-24 g during 18 days.

EFFECT: enhanced effectiveness in preserving chickens in post-embryonic period.

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Description

The invention relates to the field of agriculture and, in particular, to poultry farming (the complex use of ozone, succinic and para-aminobenzoic (PABA) acids).

The use of ozone for disinfection and stimulation of embryogenesis of agricultural production is known. birds [1].

A known method of stimulating the development of bird embryos by exposure to PABA self-evaporation in the gaseous phase [2].

These methods, taken as a prototype, consist in the fact that hatching eggs are treated either with ozone alone or only with PABA pairs. However, when using each of these methods, the hatching of chickens increased by no more than 2-4%, and the growth-promoting effect in the post-embryonic period lasted no more than 1-2 weeks.

The purpose of the invention is to significantly increase the hatchability of eggs and improve the safety of chickens in the postembryonic period by complex treatment with ozone, 0.3% solution of succinic acid (UC) and PABA (against the background of excluding pre-incubation treatment with formaldehyde).

The method is as follows.

Before laying in the incubator, the eggs are ozonized in an Antares ozonizer type chamber with a maximum concentration of 343 mg / m ^{3 of} ozone for 60 minutes.

Using a jet aerosol generator SAG-1, the eggs are aerosolized after 5-6 hours of heating them in an incubator with a 0.2-0.3% solution of UC. Aerosol exposure is carried out in the descamer at a temperature of 18-22 ° C at the rate of 1.5-2 ml / m ³ followed by exposure in an aerosol cloud for no more than 30 minutes. As a result of this, egg cooling is observed, due to which the aerosol preparation is suctioned by vacuum.

A Petri dish with 10 g PABA is placed in the incubation cabinet for 18 days. At a temperature of 37.6-37.7 ° C, intense evaporation of the drug occurs with a calculated treatment dose of 10 ^-22 -10 ^-24 g.

Example 1. Hatching eggs of the cross "Lohmann brown" obtained from one parent herd, subject to equality of weight, time of demolition and shelf life. The number of eggs in each batch is 816 pieces. The eggs of the experimental batch were processed according to the above method.

As a control, eggs of the same batch treated only with ozone were used (according to farm technology).

The data obtained are presented in Table 1: hatchability of eggs and hatching in the experimental batch increased by 5.9% (p <0.05) and 7.1% (p <0.01), respectively, compared with the control.

Example 2. Chickens hatched from processed eggs (Example 1) were raised under the conditions of the same farm until 67 days of age. The data are presented in table 2, the chicks' waste for 67 days of rearing decreased by 1.4 times compared with the control.

Example 3. The incubation eggs of hens of the cross "Lohmann brown" obtained from one house for two days of collection, sorted into trays. The treatment was carried out in accordance with example 1. The control batch was processed according to the farm scheme (formaldehyde), 1 experimental batch - ozone + 0.03% solution of YAK + PABA, and 2 - PABA. The data obtained are presented in table.3. The hatchability of eggs and hatching in 1 and 2 experimental lots were 8.4% (p <0.001) and 13% (p <0.001); 4% and 2.9%, respectively, are higher than in the control.

Example 4. In chickens hatched from treated eggs (Example 1) at the age of 24 hours, a biochemical study of the liver and blood serum showed an increase in ATP content, the activity of the enzyme ATPase and succinate dehydrogenase (SDH), respectively, by 14.7%, 77.8% ( p <0.05) and 6.3% compared with the control. For other indicators, the differences were insignificant (table. 4.).

Table 1 Lots of eggs Number of eggs Egg hatchability,% Δ,% The output of chickens,% Δ,% Control 816 72.6 ± 1.56 - 59.2 ± 1.72 - Experienced 816 78.5 ± 1.44 * +5.9 66.3 ± 1.65 ** +7.1 * p <0.05; ** - p <0.01

table 2 Groups of chickens Number of chickens, goal The waste of chickens for days of cultivation,% Control 150 5 Experienced 150 3,7

Table 3 Lots of eggs Number of eggs Egg hatchability,% Δ,% Conclusion
chickens,% Δ,% Control 408 79.9 ± 1.98 - 66.2 ± 2.34 - 1 Experienced 408 88.3 ± 1.59 * 8.4 79.2 ± 2.01 * 13.0 2 Experienced 408 83.9 ± 1.82 4.0 69.1 ± 2.29 2.9 * p <0.001

Table 4 Indicators Control Experienced Serum Total Protein 36.67 ± 0.15 36.76 ± 0.14 The content of ATP in the liver of chickens, mg% 1.63 ± 0.077 1.87 ± 0.098 The activity of the enzyme ATPase in the liver of chickens R / g / min 0.09 ± 0.029 0.16 ± 0.079 * The activity of LDH in the liver of chickens, N mol of succinate / min 1.9 ± 0.072 2.02 ± 0.031 Activity in blood serum AlAT usled 4.15 ± 0.102 4.32 ± 0.135 Activity in serum 51.01 ± 0.163 51.22 ± 0.147

Literature:

Krivopishin I.P. "Ozone in industrial poultry" M., Rosagropromizdat, 1998. - P.96

Shangin-Berezovsky G.N., Moloskin S.A., Ryhletskaya O.S. et al. “Comparative study of the effects of NDMM and PABA in experiments on stimulating the development of chickens. Chemical mutagenesis and agricultural quality products. " M., 1983. - p. 252-257.

Claims (1)

The method of disinfection and stimulation of embryonic development of poultry by processing eggs, characterized in that the eggs are aerated with ozone at a rate of 343-350 mg / m ³ , followed by aerosol treatment with 0.2-0.3% succinic acid in a dose of 1.5- 2 ml / m ³ for 30-35 min, then exposed to pairs of para-aminobenzoic acid in an incubation cabinet for 18 days.