RU2252768C1 - Method for production of peloid water extract - Google Patents

Abstract

FIELD: method for production of bioproducts from native divot peloid with high biological activity.

SUBSTANCE: claimed method includes extraction of fine dispersed divot peloid having plant residual decomposition ratio of 50 % or more with sodium hydrocarbonate-containing water at elevated temperature under intensive agitation to produce homogeneous suspension followed by separation under certain conditions.

EFFECT: ecologically pure concentrated peloid extract with high biological activity.

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Description

The invention relates to a method for producing bioproducts, in particular water extracts from native peat mud, and can be widely used in veterinary medicine, medicine, agriculture - crop production, horticulture, animal husbandry, poultry farming.

A known method of obtaining an extract from sludge therapeutic mud, including grinding the mud with a moisture content of 50-60% to a pasty state, its extraction with 96% ethyl alcohol twice with a ratio of 6-10 volumes of alcohol per 1 kg of raw material at a temperature of 30-50 ° C and intense stirring for 20-25 minutes, sedimenting the mud mass for 3-4 hours, after which the alcohol micelle is drained. Vaseline oil is added to the fused micelle in an amount ensuring the extract of the desired concentration, and distillation of the alcohol is carried out under a vacuum of 05-07 atm at a temperature not exceeding 50 ° C with periodic stirring. The remaining extract is defended (the waste is mainly salt water), and then dissolved in liquid paraffin (RU, p. No. 2073999, A 61 K 35/10).

The disadvantage of this method is the incomplete extraction of useful substances in connection with oxidative processes that occur during extraction in alcohol, as well as with subsequent distillation of alcohol with the addition of paraffin oil, which has poor absorption and poor extraction. Inadequate temperature of 30-50 ° C also contributes to a decrease in the percentage of release of biologically active substances into the extract.

A known method of obtaining an extract having fungicidal activity from low-lying sedimentary peat, including freezing peat at a temperature of -20 ° C for 72 hours, extraction with water heated to 90 ° C for 2 hours, with a ratio of raw material: extractant 1: 12-15 filtering, after which the extract is evaporated to 1/5 of the volume, and purification of the target product by precipitation with ethyl alcohol (RU, p. No. 2036647, A 61 K 35/10).

This method is inherent in the main disadvantage characteristic of the first method, namely: obtaining an extract with low biological activity. A decrease in biological activity is also promoted by the high temperature of 90 ° С extraction, in which part of the biologically active substances is destroyed.

The closest technical solution to the claimed is a method of obtaining an aqueous extract (mother liquor of sodium humate) from peat, including the extraction of ground peat in an alkaline medium - an aqueous solution of NaOH at a temperature of 80-90 ° C and periodic stirring for 1 hour, draining the finished product in storage capacity by centrifugation followed by settling for 3-4 hours (RU, p. No. 2177021, C 10 F 7/00).

The main disadvantages of this method are similar to the disadvantages of the first and second analogues. In addition, a significant drawback is the fact that an aqueous solution of NaOH is used as the extractant, i.e., acid-base hydrolysis is carried out, which causes strong, destructive physicochemical effects on the native structure of the fractions of humic compounds (FGS).

The technical result of the present invention is to obtain an environmentally friendly, concentrated extract of therapeutic mud with high biological activity.

The technical result is achieved by the fact that the method of obtaining an aqueous extract of therapeutic mud involves the extraction of finely divided therapeutic mud in an alkaline environment at elevated temperature and intensive mixing until a homogeneous suspension is obtained, followed by its separation, while peat mud with a degree of decomposition of plant residues 50 is used as a therapeutic mud % or more, and the extraction is carried out with 7-9 volumes of sodium bicarbonate mineral water at a temperature of 65-70 ° C, the suspension is separated by settling curing for 36-48 hours with the selection of the liquid phase, followed by filtration of the liquid phase through a fine mesh filter and centrifuging it at a speed of 2500-3000 rpm

In the inventive method, peat mud is used with a degree of decomposition of plant residues of 50% or more, the extraction of which is carried out at a temperature of 65-70 ° C.

It is such peat that is highly mineralized and rich in organic substances with a high content of aromatic carbon atoms.

When such peat is extracted with natural hydrocarbonate sodium mineral water of the Khabarovskaya 1 type, the pentocyclic hydrocarbon С ₃₀ Н ₅₀ , which belongs to the new class of triterpinoids, is fully extracted, which serves as a new allotropic form of hydrocarbon and is included in the so-called fullerene class of materials or preparations, a distinctive feature which is a high degree of aromaticity, which increases the biological activity of the finished product. Pentocyclic hydrocarbon C ₃₀ H ₅₀ is recovered only in a slightly alkaline medium at pH 6-8.

Furthermore, the extraction of various groups of extracted hydrocarbons aromatic structure, a high content suggested by the data fragmented composition of the extract claimed nmr spectra ¹³ C obtained in chemistry laboratories and wood Irkutsk Institute of Chemistry and listed in the table.

The temperature of extraction of raw materials 65-70 ° C is the optimum temperature at which decomposition and loss of biologically active substances is excluded.

The solution of the problem contributes to the separation of the suspension in three stages:

- sedimentation for 36-48 hours with the release of the liquid phase 36-48 hours - this is time sufficient to maximize the separation of the whole complex of biologically active substances from raw materials and to achieve a complete, final form of formation and interaction of active substances passing into the finished product, making it more saturated and concentrated;

- filtration of the liquid phase of the suspension through a fine-mesh filter after settling and subsequent centrifugation at a speed of 2500-3000 rpm, provide final separation of the raw materials and purification from any mechanical impurities to obtain an environmentally friendly concentrated extract.

At the same time, the optimal parameters of technological operations - the time of suspension sedimentation 36-48 hours, the extraction temperature of 65-70 ° C and the ratio of raw materials to extractant 1: 7-9 was determined taking into account the degree of intensity of extraction of the organic component of the raw material and extractant, since when using peat raw materials the extraction is predominantly organic component.

To prepare an aqueous extract of therapeutic mud, native peat mud with a degree of decomposition of 50% or more is used as a therapeutic mud, as well as a paste made from the above mud, for example, Kiinskaya peat mud paste (TU 9365-014-2698312-2001) , Bichevskoye deposit, Khabarovsk Territory.

As the extractant, sodium bicarbonate mineral water such as “Khabarovskaya 1”, “Mukhenskoye field”, Khabarovsk Territory (TU 10 RSFSR 363) or any mineral water similar in composition to the above mineral water is used.

The extract is prepared as follows.

Example 1

The Kiinskaya peat paste and the Khabarovskaya 1 mineral hydrocarbonate-sodium water in a ratio of 1: 7 are placed in a mixer-extractor, mixed at a temperature of 65 ° C until a homogeneous suspension is obtained. The suspension is poured into plastic containers or barrels with a polyethylene liner, defended for 36-48 hours. Next, the liquid phase of the suspension is poured into another container and filtered through a fine-mesh filter, after which it is subjected to centrifugation at a speed of 2500-3000 rpm, obtaining a pure aqueous extract without any mechanical impurities, saturated with biologically active substances with the following parameters. Organoleptic and physico-chemical:

1. Appearance The liquid is opaque, dark brown 2. Impurities Absence, a small natural precipitate of humic and mineral substances is allowed. 3.Smell Specific peat up to 3 points 4. The indicator of the concentration of hydrogen ions (pH) 6.0-8.0 5. The total concentration of water-soluble humic-mineral substances, g / l 3.5-4.0 6. The concentration of phenols, ml / l No more than 0,001 Microbiological: 1. Permissible rates of microbial contamination (OMC) Not more than 10 ³ per 1 g of product 2. Mold fungi, Candida mushrooms Not more than 10 ² per 1 g of product 3. Heavy metals (amount), including: No more than 20 mg / kg - lead 5 mg / kg - arsenic 5 mg / kg - mercury 1 mg / kg

Designation The content of C in structural fragments q _x ,% rel. The range of chemical shifts (CS) δ, ppm, the assignment of signals C = O Carbon atoms of carbomyl groups 4.4 220-186 СО _chin Carbon atoms of quinoid groups 9.8 186-180 C (O) OH, C (O) OR Carbon atoms of carboxyl, ester groups 15,2 185-168 C _ap - O Aromatic carbon atoms bound to oxygen atoms 17.9 164-140 C _ar C, H C and H substituted aromatic carbon atoms 38,4 140-103 Cl _{carbohydrates} Anomeric carbon atoms 5.1 103-96 С _{α, β -O-4} Carbon atoms in α -О-4 and β -О-4-bonds of “lignin” components 3.6 93-80 CHO, CH ₂ O Carbon atoms of —CH — O, —CH — N fragments 5.5 80-58 CH ₃ O Carbon atoms of methoxy groups 0 58-54 C _alk Carbon atoms of alkyl fragments 0.1 54-20 Aromaticity (fa) fa = Iap / I _total 57 164-103

The solid sediment of the mud suspension can be used as a standalone product for skin applications.

Example 2

The method is carried out as in example 1, only native peat with a natural moisture content of 60-80% and a degree of decomposition of plant residues of 50% or more and 8 parts of Khabarovskaya 1 mineral water is placed in the mixer-extractor, and the suspension is mixed at a temperature of 67 ° C. The indicators of the obtained extract correspond to the indicators of example 1.

Example 3

The method is carried out as in example 2, only the ratio of raw materials: extractant is taken 1: 9, and the suspension is mixed at a temperature of 70 ° C. The indicators of the obtained extract correspond to those of example 1.

Silt mud (sapropel) of the freshwater lakes of the Amur region can also be used as feedstock by this method.

The inventive method, which allows to obtain an environmentally friendly, biologically active drug prepared from natural raw materials and having a wide spectrum of action, will find, according to the author, wide industrial application.

Claims (1)

A method of obtaining an aqueous extract of therapeutic mud, including the extraction of finely dispersed therapeutic mud in an alkaline medium at elevated temperature and vigorous stirring to obtain a homogeneous suspension with its subsequent separation, characterized in that peat mud with a degree of decomposition of plant residues of 50% or more is used as a therapeutic mud the extraction is carried out with 7-9 volumes of sodium bicarbonate mineral water at a temperature of 65-70 ° C, the suspension is separated by settling for 36-48 hours liquid phase followed by filtration of the liquid phase through a fine mesh filter and centrifugation at a speed of 2500-3000 rpm