RU2006118439A - Process for the preparation of genetically engineered HUMAN EPIDERMAL GROWTH FACTOR, with recombinant plasmid DNA pER-hEGF, encode fusion protein capable of autocatalytic cleavage WITH FORMATION OF HUMAN EPIDERMAL GROWTH FACTOR AND STRAIN Escherichia coli ER2566 / pER-hEGF - PRODUCER said protein - Google Patents
Process for the preparation of genetically engineered HUMAN EPIDERMAL GROWTH FACTOR, with recombinant plasmid DNA pER-hEGF, encode fusion protein capable of autocatalytic cleavage WITH FORMATION OF HUMAN EPIDERMAL GROWTH FACTOR AND STRAIN Escherichia coli ER2566 / pER-hEGF - PRODUCER said protein Download PDFInfo
- Publication number
- RU2006118439A RU2006118439A RU2006118439/13A RU2006118439A RU2006118439A RU 2006118439 A RU2006118439 A RU 2006118439A RU 2006118439/13 A RU2006118439/13 A RU 2006118439/13A RU 2006118439 A RU2006118439 A RU 2006118439A RU 2006118439 A RU2006118439 A RU 2006118439A
- Authority
- RU
- Russia
- Prior art keywords
- hegf
- growth factor
- per
- epidermal growth
- human epidermal
- Prior art date
Links
- 239000013612 plasmid Substances 0.000 title claims 7
- 241000588724 Escherichia coli Species 0.000 title claims 6
- 101500025419 Homo sapiens Epidermal growth factor Proteins 0.000 title claims 5
- 229940116978 human epidermal growth factor Drugs 0.000 title claims 5
- GVUGOAYIVIDWIO-UFWWTJHBSA-N nepidermin Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H](CS)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CS)NC(=O)[C@H](C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C(C)C)C(C)C)C1=CC=C(O)C=C1 GVUGOAYIVIDWIO-UFWWTJHBSA-N 0.000 title claims 5
- 108090000623 proteins and genes Proteins 0.000 title claims 4
- 108020001507 fusion proteins Proteins 0.000 title claims 3
- 102000037865 fusion proteins Human genes 0.000 title claims 3
- 102000004169 proteins and genes Human genes 0.000 title claims 3
- 238000003776 cleavage reaction Methods 0.000 title claims 2
- 238000000034 method Methods 0.000 title claims 2
- 230000007017 scission Effects 0.000 title claims 2
- 230000015572 biosynthetic process Effects 0.000 title 1
- 210000004027 cell Anatomy 0.000 claims 3
- 229920002101 Chitin Polymers 0.000 claims 2
- 108090000204 Dipeptidase 1 Proteins 0.000 claims 2
- 239000000872 buffer Substances 0.000 claims 2
- 239000012634 fragment Substances 0.000 claims 2
- 239000002594 sorbent Substances 0.000 claims 2
- 101150072531 10 gene Proteins 0.000 claims 1
- 102000009024 Epidermal Growth Factor Human genes 0.000 claims 1
- 101800003838 Epidermal growth factor Proteins 0.000 claims 1
- 101150084044 P gene Proteins 0.000 claims 1
- 229930182555 Penicillin Natural products 0.000 claims 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims 1
- 101710137500 T7 RNA polymerase Proteins 0.000 claims 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims 1
- 239000003242 anti bacterial agent Substances 0.000 claims 1
- 229940088710 antibiotic agent Drugs 0.000 claims 1
- 239000007853 buffer solution Substances 0.000 claims 1
- 239000004202 carbamide Substances 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 230000006378 damage Effects 0.000 claims 1
- 239000003623 enhancer Substances 0.000 claims 1
- 229940116977 epidermal growth factor Drugs 0.000 claims 1
- 230000002068 genetic effect Effects 0.000 claims 1
- 239000003102 growth factor Substances 0.000 claims 1
- 210000003000 inclusion body Anatomy 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- 229940049954 penicillin Drugs 0.000 claims 1
- 108091008146 restriction endonucleases Proteins 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
- 238000013518 transcription Methods 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
- 238000002604 ultrasonography Methods 0.000 claims 1
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 claims 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2006118439/13A RU2323976C2 (en) | 2006-05-30 | 2006-05-30 | METHOD OF OBTAINING OF GENI-ENGENIERING ECDERONIC FACTOR OF PERSON GROWTH, PLASM RECOMBINATION DNA pER-hEGF, CODING HYBRID PROTEIN CAPABLE FOR AUTO-CATALYTIC SPLITTING, CAPABLE FOR AUTO-CATALYTIC SPLITTING WITH GENERATING OF EPIDERMAL FACTOR OF PERSON GROWTH, AND STAMP Escherichia coli ER2566/pER-hEGF-PRODUCER OF MENTIONED PROTEIN |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2006118439/13A RU2323976C2 (en) | 2006-05-30 | 2006-05-30 | METHOD OF OBTAINING OF GENI-ENGENIERING ECDERONIC FACTOR OF PERSON GROWTH, PLASM RECOMBINATION DNA pER-hEGF, CODING HYBRID PROTEIN CAPABLE FOR AUTO-CATALYTIC SPLITTING, CAPABLE FOR AUTO-CATALYTIC SPLITTING WITH GENERATING OF EPIDERMAL FACTOR OF PERSON GROWTH, AND STAMP Escherichia coli ER2566/pER-hEGF-PRODUCER OF MENTIONED PROTEIN |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2006118439A true RU2006118439A (en) | 2007-12-10 |
| RU2323976C2 RU2323976C2 (en) | 2008-05-10 |
Family
ID=38903532
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2006118439/13A RU2323976C2 (en) | 2006-05-30 | 2006-05-30 | METHOD OF OBTAINING OF GENI-ENGENIERING ECDERONIC FACTOR OF PERSON GROWTH, PLASM RECOMBINATION DNA pER-hEGF, CODING HYBRID PROTEIN CAPABLE FOR AUTO-CATALYTIC SPLITTING, CAPABLE FOR AUTO-CATALYTIC SPLITTING WITH GENERATING OF EPIDERMAL FACTOR OF PERSON GROWTH, AND STAMP Escherichia coli ER2566/pER-hEGF-PRODUCER OF MENTIONED PROTEIN |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2323976C2 (en) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2248983C1 (en) * | 2003-08-18 | 2005-03-27 | Позмогова Галина Евгеньевна | Peptide vector, method for its preparing, nucleotide sequence, recombinant plasmid dna and strain escherichia coli b-8389 vkpm for its preparing, method for genetic modification of mammalian and human cells |
-
2006
- 2006-05-30 RU RU2006118439/13A patent/RU2323976C2/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| RU2323976C2 (en) | 2008-05-10 |
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| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20110531 |
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| NF4A | Reinstatement of patent |
Effective date: 20120610 |
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| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20170531 |