RU1836095C - Mode to obtain the drug for treatment gastro-intestinal disorders - Google Patents

Abstract

Usage: in medicine, in particular in the technology of medicines. SUBSTANCE: ascorbic acid and 9-21 g of block pectin per initial volume are added to the obtained extract after filtration, while extraction is carried out at a raw material-water ratio of 1:30. Positive effect: the method allows to increase the yield of the target product, and in addition, the target product has antidote and radionuclide binding properties. 1 ill., 2 tablets

Description

The invention relates to medicine, in particular to the technology of drugs.

The aim of the invention is to increase the stability of the target product and give it anti-acid and radionuclide-binding properties.

This goal is achieved by using 9-21 g of block pectin, which is introduced into a decoction of a sheet of plantain prepared in a ratio of 10: 300, moreover, the decoction of a sheet of plantain is pre-stabilized with ascorbic acid 0.25 g.

} The method is carried out as follows: J30M.

I For the extraction of biologically active substances from boletus leaves, boiling water is used. The boiled leaves of boletus majora are placed in boiled water and boiled for 25-30 minutes with slight heating. Then the broth is cooled for 20-30 minutes and filtered. The remaining raw material is squeezed in. The volume of the broth is adjusted with water to a predetermined value.

The broth is then stabilized. Ascorbic acid is dissolved in the resulting broth, and then block pectin is dissolved in the stabilized broth.

An example of a specific implementation of the goal can be the study of three options for the composition of the agent (Table 1).

Biologically active substances extracted from the leaves of plantain: pectin, flavonoids, iridoids, saponins, etc. Pectin, saponins, flavonoids and hydroxycinnamic acids help to reduce blood cholesterol and have a wound healing and hypocholesterolemic effect. Iridoid glycosides aucubin, catalpol, etc., as well as hydroxycinnamic acids.

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you have a pronounced anti-inflammatory effect (2,3),

In order to stabilize iridoid glycosides and hydroxycinnamic acids, which are among the most unstable compounds, we included ascorbic acid in the composition of the claimed agent.

However, it is known that ascorbic acid itself is stable only in a dry state (crystalline form), while in solution it is easily oxidized.

Ethylene diamine-tetraacetic acid, thioacetals, some N-heterocyclic compounds, and others inhibit the reaction of ascorbic acid oxidation in solutions.

To stabilize the ascorbic acid, which is part of the claimed agent and to achieve its storage stability, we used block pectin.

The drawing shows the effect of pectin on the stability of ascorbic acid in aqueous solutions.

The following symbols are given in the drawing:

1–5% block pectin solution prepared on a decoction of plantain leaf (the claimed agent);

2-3% block pectin solution prepared on a decoction of plantain leaf;

3-2% block pectin solution prepared on a decoction of plantain leaf; .-

4-5% block pectin solution;

5-1% block pectin solution;

6 - water.

As shown by the data presented in the drawing, the highest stability of ascorbic acid was achieved in the claimed agent (composition No. 2), where its constant concentration was observed for 12 months. The concentration of ascorbic acid was monitored at optical density at A 265 nm. This variant of the prophylactic is also optimal for industrial production. Composition 3 is less convenient due to the high viscosity of the solution.

To confirm the usefulness of the claimed technical solution, the authors additionally carried out clinical and experimental studies confirming the claimed properties of the agent. Its long-term effects on the body of immature rats have been investigated.

When setting up and conducting the experiment, we met the requirements in accordance with the Guidelines for the study of the carcinogenic properties of chemicals and biological products in chronic animal experiments. M.-L., 1981, as well as the Guidelines for the experimental and clinical study of new antibacterial drugs and antibiotics, M., 1970.

The search for methods and approaches should not be standard, since the studied product contains various biological, chemical or physical compounds with different effects on the body.

Therefore, when conducting a long experiment with the claimed substance, we paid special attention to studying the possibility of its effect on the function of the liver, kidneys, gastrointestinal tract, redox reactions, and other indicators.

Immature young individuals (t-weaned rats), the organism of which with an immune and nervous system that has not yet been restored and easily exposed to exo and enzofactors, were taken into the experiment. Group 1 — control animals were kept on a standard diet of Vivarium, and group 11 — animals received a daily oral dose of 120 mg / kg body weight. The duration of the experiment is 1 month.

Studies have shown that the most characteristic of physiological indicators was the data on the protein efficiency coefficient (CEB), which characterizes the degree of metabolic processes in the body, i.e. the growth and development of a young organism that is most sensitive to the effects of possible negative factors (Table 2).

The data of Table 2 indicate that the experimental animals of the I group receiving the indicated agent grew and developed quite intensively. The increase in body weight tended to increase. Animals had a good appetite, a quick reaction to external stimuli. Coat smooth, shiny. This is explained by rather intense metabolic processes at the stage of physiological and puberty, which is confirmed by a high protein metabolic index (CEB).

In addition, the effect of the claimed agent on the body of patients living in areas contaminated with radionuclides and in contact with microbiological and chemical synthesis products was studied.

j The indicated drug was prescribed up to 50 ml 2-3 times a day for 1-8-20 days. Repeated examination of patients revealed that the dynamics of clinical manifestations were positive (: see the act of approbation).

 Thus, the results of experimental and clinical studies carried out at the Department of Pharmaceutical Chemistry made it possible to recommend an antidote and radionuclide-producing properties for prophylactic purposes and as a product of therapeutic nutrition in diseases of the gastrointestinal tract. moreover, the means

good at storage, while maintaining healing and preventive properties. Method and method for preparing an agent for treating diseases of the gastrointestinal tract by water extraction of plantain leaf and filtering it, characterized in that that, in order to increase the stability of the target product and give it antidote and radionuclide-binding properties, extraction is carried out at a ratio of raw materials: water of 1:30, after filtration, the extract is stabilized with ascorbic acid, then 9-21 g of block pectin are added to it initial volume.

Table 1

table 2

0.2

S

Continuation of the table. 2