[go: up one dir, main page]

PL2635595T3 - Lucyferazy otrzymane z krewetki oplophorus, nowe substraty koelenterazyny oraz sposoby stosowania - Google Patents

Lucyferazy otrzymane z krewetki oplophorus, nowe substraty koelenterazyny oraz sposoby stosowania

Info

Publication number
PL2635595T3
PL2635595T3 PL11785534T PL11785534T PL2635595T3 PL 2635595 T3 PL2635595 T3 PL 2635595T3 PL 11785534 T PL11785534 T PL 11785534T PL 11785534 T PL11785534 T PL 11785534T PL 2635595 T3 PL2635595 T3 PL 2635595T3
Authority
PL
Poland
Prior art keywords
coelenterine
luciferases
substances
ways
received
Prior art date
Application number
PL11785534T
Other languages
English (en)
Other versions
PL2635595T4 (pl
Inventor
Brock Binkowski
Lance P. Encell
Mary Hall
Matthew B. Robers
Michael R. Slater
Keith V. Wood
Monika G. Wood
Dieter Klaubert
Poncho Meisenheimer
James Unch
Michael P. Valley
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44983728&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PL2635595(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Promega Corp filed Critical Promega Corp
Publication of PL2635595T4 publication Critical patent/PL2635595T4/pl
Publication of PL2635595T3 publication Critical patent/PL2635595T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12005—Renilla-luciferin 2-monooxygenase (1.13.12.5), i.e. renilla-luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12013—Oplophorus-luciferin 2-monooxygenase (1.13.12.13)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
PL11785534T 2010-11-02 2011-11-02 Lucyferazy otrzymane z krewetki oplophorus, nowe substraty koelenterazyny oraz sposoby stosowania PL2635595T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US40942210P 2010-11-02 2010-11-02
PCT/US2011/059018 WO2012061530A2 (en) 2010-11-02 2011-11-02 Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
EP11785534.6A EP2635595B1 (en) 2010-11-02 2011-11-02 Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use

Publications (2)

Publication Number Publication Date
PL2635595T4 PL2635595T4 (pl) 2019-04-30
PL2635595T3 true PL2635595T3 (pl) 2019-04-30

Family

ID=44983728

Family Applications (2)

Application Number Title Priority Date Filing Date
PL11782732T PL2635582T3 (pl) 2010-11-02 2011-11-02 Nowe substraty będące koelenterazynami i sposoby zastosowania
PL11785534T PL2635595T3 (pl) 2010-11-02 2011-11-02 Lucyferazy otrzymane z krewetki oplophorus, nowe substraty koelenterazyny oraz sposoby stosowania

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PL11782732T PL2635582T3 (pl) 2010-11-02 2011-11-02 Nowe substraty będące koelenterazynami i sposoby zastosowania

Country Status (15)

Country Link
US (10) US8809529B2 (pl)
EP (4) EP3498709A1 (pl)
JP (6) JP6155424B2 (pl)
KR (2) KR101538624B1 (pl)
CN (3) CN103370319B (pl)
AU (2) AU2011323418B2 (pl)
BR (2) BR112013010855A2 (pl)
CA (3) CA3081115A1 (pl)
DK (2) DK2635582T3 (pl)
ES (2) ES2567410T3 (pl)
IL (5) IL225867B (pl)
PL (2) PL2635582T3 (pl)
PT (1) PT2635595T (pl)
SG (5) SG190104A1 (pl)
WO (2) WO2012061530A2 (pl)

Families Citing this family (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2298902B1 (en) 2003-10-10 2015-04-22 Promega Corporation Luciferase biosensor
JP5684984B2 (ja) 2006-04-03 2015-03-18 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 順列置換及び非順列置換ルシフェラーゼバイオセンサー
ATE542903T1 (de) 2008-05-19 2012-02-15 Promega Corp Luciferase-biosensoren für camp
KR101665352B1 (ko) 2009-05-01 2016-10-12 프로메가 코포레이션 광 출력이 향상된 합성 오플로포러스 루시퍼라제
US9290794B2 (en) * 2010-05-11 2016-03-22 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
EP2569423B1 (en) 2010-05-11 2015-12-02 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
EP2635583B1 (en) 2010-11-02 2015-06-03 Promega Corporation Coelenterazine derivatives and methods of using same
AU2011323418B2 (en) 2010-11-02 2017-07-27 Promega Corporation Novel coelenterazine substrates and methods of use
EP2782916B1 (en) 2011-11-21 2018-02-28 Promega Corporation Carboxy x rhodamine analogs
US10067149B2 (en) 2012-12-12 2018-09-04 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
KR102272233B1 (ko) * 2012-12-12 2021-07-01 프로메가 코포레이션 생체활성 제제의 세포 표적 포획을 위한 조성물 및 방법
US10024862B2 (en) * 2012-12-12 2018-07-17 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
JP6511720B2 (ja) * 2013-02-28 2019-05-15 Jnc株式会社 コドン最適化変異エビルシフェラーゼ遺伝子およびその使用方法
US10168323B2 (en) 2013-03-15 2019-01-01 Promega Corporation Compositions and methods for capture of cellular targets of bioactive agents
ES2926463T3 (es) * 2013-03-15 2022-10-26 Promega Corp Activación de la bioluminiscencia mediante complementación estructural
US11072811B2 (en) 2013-03-15 2021-07-27 Promega Corporation Substrates for covalent tethering of proteins to functional groups or solid surfaces
EP2978857A2 (en) 2013-03-27 2016-02-03 Sample Technologies, Inc. Recombinant phage and bacterial detection methods
JP6040846B2 (ja) * 2013-04-08 2016-12-07 Jnc株式会社 セレンテラジン類縁体
CA2915820A1 (en) 2013-06-19 2014-12-24 Michael Sandor Koeris Phage-based bacterial detection assay
RU2705249C2 (ru) * 2013-07-12 2019-11-06 Дзе Чилдрен'З Хоспитал Оф Филадельфия Вектор aav и анализ на нейтрализующие антитела против aav (аденоассоциированного вируса)
CA2918426C (en) 2013-07-18 2021-10-19 Ecole Polytechnique Federale De Lausanne (Epfl) Means and methods for bioluminescence resonance energy transfer (bret) analysis in a biological sample
JP6484987B2 (ja) * 2013-10-21 2019-03-20 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
WO2015067302A1 (en) 2013-11-05 2015-05-14 Ecole Polytechnique Federale De Lausanne (Epfl) Sensor molecules and uses thereof
JP6442825B2 (ja) 2013-12-26 2018-12-26 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
US9927430B2 (en) 2014-01-29 2018-03-27 Promega Corporation Pro-substrates for live cell applications
JP6703484B2 (ja) 2014-01-29 2020-06-03 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 細胞による取り込み測定のための、標識用試薬としての、キノンでマスクされたプローブ
JP6256118B2 (ja) * 2014-03-11 2018-01-10 Jnc株式会社 エビルシフェラーゼの触媒蛋白質のキメラ遺伝子及びその使用法
JP6265020B2 (ja) 2014-04-16 2018-01-24 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
JP6641297B2 (ja) * 2014-05-23 2020-02-05 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 発光源基質を安定させる組成物及び方法
US9732373B2 (en) 2014-09-11 2017-08-15 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
US11365402B2 (en) * 2014-09-12 2022-06-21 Promega Corporation Internal protein tags
EP3191601B1 (en) * 2014-09-12 2022-11-02 Promega Corporation Internal protein tags
WO2016098078A2 (en) * 2014-12-19 2016-06-23 Novartis Ag Dimerization switches and uses thereof
WO2016127100A1 (en) 2015-02-05 2016-08-11 Promega Corporation Luciferase-based thermal shift assays
JP6876002B2 (ja) 2015-06-05 2021-05-26 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 機能的要素を共有結合により係留させるための細胞透過性、細胞適合性、かつ開裂可能であるリンカー
EP3313846B1 (en) 2015-06-25 2020-05-06 Promega Corporation Thienopyrrole compounds and uses thereof as inhibitors of oplophorus-derived luciferases
US20170029798A1 (en) * 2015-07-27 2017-02-02 Cellivery Therapeutics, Inc. Development of Improved Cell-Permeable (iCP) Parkin Recombinant Protein as a Protein-Based Anti-Neurodegenerative Agent for the Treatment of Parkinson's Disease-Associated Phenotypes by Utilizing BBB-Penetrating Protein Delivery System MITT, Enabled by Advanced Macromolecule Transduction Domain (aMTD)
US9924073B2 (en) * 2016-02-15 2018-03-20 Promega Corporation Substituted imidazo[1,2-a]pyrazines as luciferase substrates and preparation method thereof
US11015176B2 (en) 2016-03-11 2021-05-25 National Institute Of Advanced Industrial Science And Technology Nucleic acid encoding a luminescent enzyme protein
WO2017189751A1 (en) 2016-04-26 2017-11-02 The University Of Utah Research Foundation Target-binding activated split reporter systems for analyte detection and related components and methods
CN105968114B (zh) * 2016-05-27 2018-11-09 山东大学 一种腔肠素类似物及其制备方法与应用
EP3490991B1 (en) 2016-07-28 2021-01-06 Promega Corporation Coelenterazine analogues
US10202584B2 (en) * 2016-09-01 2019-02-12 The Regents Of The University Of California Red-shifted luciferase-luciferin pairs for enhanced bioluminescence
US10316070B2 (en) 2016-09-09 2019-06-11 Promega Corporation Dual protected pro-coelenterazine substrates
JP7200102B2 (ja) 2016-11-01 2023-01-06 プロメガ コーポレイション エネルギー受容体に係留されたセレンテラジン類縁体
DK3548492T5 (en) * 2016-12-01 2024-08-05 Promega Corp Cell impermeable coelenterazine analogues
EP3548470B1 (en) * 2016-12-01 2023-11-01 Promega Corporation 5,5-disubstituted luciferins and their use in luciferase-based assays
JP7161475B2 (ja) 2016-12-28 2022-10-26 プロメガ コーポレイション 官能化ｎａｎｏｌｕｃ阻害剤
WO2018219953A1 (en) 2017-05-30 2018-12-06 Ecole polytechnique fédérale de Lausanne (EPFL) Sensors, methods and kits for detecting nadph based on resonance energy transfer.
ES2983923T3 (es) 2017-07-06 2024-10-28 Promega Corp Análogos de la pro-coelenterazina estables en suero
CN108107209B (zh) * 2017-09-19 2020-07-24 中国中医科学院医学实验中心 一种检测分泌型报告蛋白含量的优化方法及其所用生物材料
WO2019213119A1 (en) * 2018-05-01 2019-11-07 Promega Corporation Coelenterazine compounds as nanoluc suicide substrates
US11442063B2 (en) 2018-05-30 2022-09-13 Promega Corporation Broad spectrum kinase binding agents
US11390599B2 (en) 2018-06-01 2022-07-19 Promega Corporation Inhibitors of oplophorus luciferase-derived bioluminescent complexes
JP2021526832A (ja) * 2018-06-12 2021-10-11 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 多分子ルシフェラーゼ
US10962538B2 (en) 2018-06-14 2021-03-30 The Trustees Of Columbia University In The City Of New York Assays using arrestin recruitment and unmodified receptors
WO2020023871A1 (en) 2018-07-27 2020-01-30 Promega Corporation Quinone-containing conjugates
WO2020041579A1 (en) * 2018-08-23 2020-02-27 Promega Corporation Coelenterazine analogues
CN119955899A (zh) 2018-10-03 2025-05-09 普洛麦格公司 用于稳定腔肠素及其类似物和衍生物的组合物、方法和试剂盒
JP7703446B2 (ja) 2018-11-28 2025-07-07 プロメガ コーポレイション 反応性ペプチド標識付け
JP7634479B2 (ja) 2018-12-04 2025-02-21 プロメガ コーポレイション 広域スペクトルｇｐｃｒ結合剤
WO2020176755A1 (en) * 2019-02-27 2020-09-03 University Of Virginia Patent Foundation Atp-independent bioluminescent reporter variants to improve in vivo imaging
JP7584433B2 (ja) 2019-03-20 2024-11-15 プロメガ コーポレイション 光親和性プローブ
US11360096B2 (en) 2019-05-16 2022-06-14 Duke University Complex BRET technique for measuring biological interactions
CN114450326B (zh) 2019-06-24 2025-02-11 普罗美加公司 用于将生物分子递送到细胞中的改性聚胺聚合物
BR112022010153A2 (pt) 2019-11-27 2022-08-09 Promega Corp Peptídeos, ácido nucleico, polipeptídeo, composição, complexo bioluminescente, métodos, dipeptídeo, sistema e kit
EP4073512B1 (en) 2019-12-10 2025-11-12 Promega Corporation Compositions and methods for bioluminescent detection using multifunctional probes
EP3882632A1 (en) 2020-03-18 2021-09-22 Universite De Bordeaux Novel ion conducting channel fusion subunits and methods of use thereof
CN113527231A (zh) * 2020-04-15 2021-10-22 深圳华大生命科学研究院 杂环取代腔肠素类化合物的合成方法
EP3904508A1 (en) 2020-04-27 2021-11-03 Institut Pasteur Luciferase linked immunosorbent assay
US20210363565A1 (en) 2020-05-22 2021-11-25 Promega Corporation Enhancement of kinase target engagement
US20220064696A1 (en) 2020-08-28 2022-03-03 Promega Corporation Target engagement assay for ras proteins
EP4257978A4 (en) 2020-12-25 2024-11-13 Chugai Seiyaku Kabushiki Kaisha METHOD FOR SCREENING CANDIDATE MOLECULES CAPABLE OF FORMING A COMPLEX IN CONJUNCTION WITH MULTIPLE TARGET MOLECULES
CN118703457A (zh) * 2021-04-01 2024-09-27 深圳华大生命科学研究院 一种刺虾荧光素酶Nluc的突变体及其应用
CN118139895A (zh) * 2021-08-24 2024-06-04 巴斯德研究所 基于发光的抗原测定法
US20230357266A1 (en) 2022-05-04 2023-11-09 Promega Corporation Photoactivatable compounds and uses thereof
US20240174992A1 (en) 2022-05-04 2024-05-30 Promega Corporation Split modified dehalogenase variants
JP2025515179A (ja) 2022-05-04 2025-05-13 プロメガ コーポレイション 円順列置換デハロゲナーゼバリアント
JP2025515181A (ja) 2022-05-04 2025-05-13 プロメガ コーポレイション 伸長表面ループ領域を有する修飾デハロゲナーゼ
CN115349611B (zh) * 2022-08-05 2023-10-24 江南大学 一种改善南美白对虾虾糜凝胶品质的方法
CN116068198B (zh) * 2022-11-30 2024-01-09 深圳湾实验室 Ppi原位检测方法及其载体、诊断试剂、试剂盒和应用
US20240377404A1 (en) * 2023-03-24 2024-11-14 Promega Corporation Bioluminescence-triggered photocatalytic activation
JP7482563B1 (ja) 2023-06-21 2024-05-14 国立大学法人大阪大学 シイノトモシビタケ由来の発光関連遺伝子
DE102023120682B3 (de) 2023-08-03 2024-10-24 Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut Zytotoxizitätstest zum Nachweis von Zellschäden
GB202404021D0 (en) 2024-03-20 2024-05-01 Ipsen Biopharm Ltd Cell-based neurotoxin assay

Family Cites Families (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3477549D1 (en) 1983-08-17 1989-05-11 Allied Signal Inc Gas generator and method of generating gas
JPH0515398A (ja) 1991-07-15 1993-01-26 Nippon Oil & Fats Co Ltd オキシダーゼ酵素の定量方法
US5368484A (en) 1992-05-22 1994-11-29 Atari Games Corp. Vehicle simulator with realistic operating feedback
US6387675B1 (en) 1994-01-03 2002-05-14 Promega Corporation Mutant luciferases
DE69535744T2 (de) 1994-01-03 2009-07-02 Promega Corp., Madison Mutierte luziferasen
GB9501170D0 (en) 1994-03-23 1995-03-08 Secr Defence Luciferases
JP3648763B2 (ja) 1994-08-23 2005-05-18 日本油脂株式会社 ウミホタルルシフェリン誘導体および糖加水分解酵素の定量方法
GB9417593D0 (en) 1994-09-01 1994-10-19 Secr Defence Luciferase labelling method
US5670356A (en) 1994-12-12 1997-09-23 Promega Corporation Modified luciferase
JP4342608B2 (ja) 1995-01-20 2009-10-14 スリーエム イノベイティブ プロパティーズ カンパニー 突然変異体ルシフェラーゼ
JPH08294397A (ja) 1995-04-27 1996-11-12 Nippon Oil & Fats Co Ltd 免疫学的活性物質の測定方法
US5998204A (en) 1997-03-14 1999-12-07 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
GB9707486D0 (en) 1997-04-11 1997-05-28 Secr Defence Enzyme assays
WO1998046739A1 (fr) 1997-04-16 1998-10-22 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Molecules immunomodulatrices et leur procede de production
JP4537573B2 (ja) 1997-09-19 2010-09-01 プロメガ・コーポレイシヨン 熱安定ルシフェラーゼと生産方法
US6602677B1 (en) 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
GB9823468D0 (en) 1998-10-28 1998-12-23 Secr Defence Novel enzyme
JP3694181B2 (ja) * 1999-01-05 2005-09-14 キッコーマン株式会社 変異型ルシフェラーゼ、変異型ルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び変異型ルシフェラーゼの製造法
EP1224294B1 (en) 1999-10-26 2007-10-03 The Secretary of State for Defence Mutant luciferase
GB9925161D0 (en) 1999-10-26 1999-12-22 Secr Defence Novel enzyme
US20010046687A1 (en) 2000-03-31 2001-11-29 Dicesare Joseph L. Method for the enhancement of luminescence intensity from a reaction between adenosine triphosphate (ATP) and a luciferase/luciferin reactant system
EP1156103B1 (en) 2000-04-26 2010-08-11 Chisso Corporation Oplophorus luciferase
JP4613441B2 (ja) * 2000-04-26 2011-01-19 チッソ株式会社 新規ルシフェラーゼおよび発光蛋白質
US7118878B1 (en) 2000-06-09 2006-10-10 Promega Corporation Method for increasing luminescence assay sensitivity
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
US7268229B2 (en) 2001-11-02 2007-09-11 Promega Corporation Compounds to co-localize luminophores with luminescent proteins
EP1472238B1 (en) 2002-02-01 2012-07-04 Promega Corporation Bioluminescent protease assay
ATE513838T1 (de) 2002-09-20 2011-07-15 Promega Corp Auf lumineszenz basierende verfahren und sonden zur messung der cytochrom-p450-aktivität
ES2237643T3 (es) 2002-10-21 2005-08-01 Axxam S.R.L. Fotoproteina con bioluminiscencia mejorada.
JP4485470B2 (ja) 2002-12-23 2010-06-23 プロメガ コーポレイション ルシフェラーゼ酵素活性を保護するための方法及びキット
US7429472B2 (en) 2003-01-31 2008-09-30 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
EP1594962B1 (en) 2003-01-31 2014-08-27 Promega Corporation Covalent tethering of functional groups to proteins
EP1592805A1 (en) 2003-02-12 2005-11-09 Promega Corporation Methods and kits for dual enzymatic assays whereby light is quenched from luminescent reactions
US20040178545A1 (en) 2003-03-14 2004-09-16 Cates Larry E. System for optically analyzing a molten metal bath
EP2298902B1 (en) 2003-10-10 2015-04-22 Promega Corporation Luciferase biosensor
WO2005068483A1 (en) 2004-01-08 2005-07-28 Universität Zürich Metal complexes having vitamin b12 as a ligand
US7416854B2 (en) 2004-01-22 2008-08-26 Promega Corporation Luminogenic and nonluminogenic multiplex assay
US7553632B2 (en) 2004-01-22 2009-06-30 Promega Corporation Luminogenic and nonluminogenic multiplex assay
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
EP3179252B1 (en) 2004-07-30 2018-11-14 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
US20070087400A1 (en) 2004-07-30 2007-04-19 Aldis Darzins Covalent tethering of functional groups to proteins and substrates therefor
US7728118B2 (en) 2004-09-17 2010-06-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
JP4639904B2 (ja) 2005-03-30 2011-02-23 チッソ株式会社 蛍光活性を有するルシフェラーゼの活性増強方法
JP3844082B2 (ja) * 2005-04-08 2006-11-08 キッコーマン株式会社 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び耐熱性ホタルルシフェラーゼの製造法
EP1935986B1 (en) 2005-05-31 2014-04-16 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
WO2006130551A2 (en) 2005-05-31 2006-12-07 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
US7495579B2 (en) 2005-06-13 2009-02-24 Sirota J Marcos Traffic light status remote sensor system
MX2008002123A (es) 2005-08-17 2008-04-17 Yeda Res & Dev Metodos y composiciones para determinar un nivel de paraoxonasa de suero biologicamente activa.
JP4999341B2 (ja) * 2005-09-06 2012-08-15 株式会社バイオエネックス 変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法
JP5684984B2 (ja) * 2006-04-03 2015-03-18 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 順列置換及び非順列置換ルシフェラーゼバイオセンサー
JP2007306871A (ja) 2006-05-19 2007-11-29 Chisso Corp 封入体可溶化方法及び精製方法
CA2667697A1 (en) 2006-10-30 2008-05-08 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and functional expression
WO2008086035A2 (en) 2007-01-10 2008-07-17 Promega Corporation Split mutant hydrolase fusion reporter and uses thereof
WO2008118445A1 (en) 2007-03-26 2008-10-02 Promega Corporation Methods to quench light from optical reactions
EP2215227A2 (en) 2007-11-05 2010-08-11 Promega Corporation Hybrid fusion reporter and uses thereof
ATE542903T1 (de) 2008-05-19 2012-02-15 Promega Corp Luciferase-biosensoren für camp
US8183007B2 (en) 2008-07-22 2012-05-22 Promega Corporation ADP detection based methods using adenylate cyclase and bioluminescence
TWI375901B (en) 2008-08-05 2012-11-01 Elan Microelectronics Corp Touch screen and method for positioning coordinate
WO2010090319A1 (ja) * 2009-02-09 2010-08-12 チッソ株式会社 セレンテラジン類縁体及びその製造方法
EP2420573A1 (en) * 2009-04-17 2012-02-22 National Institute of Advanced Industrial Science And Technology Stable artificial bioluminescent enzyme having super-high brightness
KR101665352B1 (ko) * 2009-05-01 2016-10-12 프로메가 코포레이션 광 출력이 향상된 합성 오플로포러스 루시퍼라제
WO2011038219A1 (en) 2009-09-24 2011-03-31 Promega Corporation Proteases having improved stability, solubility and activity
JP5605727B2 (ja) * 2010-04-06 2014-10-15 Ｊｎｃ株式会社 セレンテラジン類縁体及びセレンテラミド類縁体
EP2569423B1 (en) 2010-05-11 2015-12-02 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
EP2611929B1 (en) 2010-09-01 2016-01-13 Promega Corporation Oxidized glutathione assay
US9284188B2 (en) 2010-09-03 2016-03-15 The Regents Of The University Of California Cytosolic delivery of materials with endosome-disrupting colloids
EP2635583B1 (en) 2010-11-02 2015-06-03 Promega Corporation Coelenterazine derivatives and methods of using same
AU2011323418B2 (en) * 2010-11-02 2017-07-27 Promega Corporation Novel coelenterazine substrates and methods of use
WO2013033515A1 (en) 2011-09-02 2013-03-07 Promega Corporation Compounds and methods for assaying redox state of metabolically active cells and methods for measuring nad(p)/nad(p)h

Also Published As

Publication number Publication date
JP5889910B2 (ja) 2016-03-22
SG10201608550SA (en) 2016-12-29
ES2723773T3 (es) 2019-09-02
KR20130084312A (ko) 2013-07-24
US20220348983A1 (en) 2022-11-03
EP2635595B1 (en) 2019-01-09
IL226114B (en) 2018-04-30
EP2635582B1 (en) 2016-01-27
WO2012061530A8 (en) 2013-06-06
EP2635595A2 (en) 2013-09-11
AU2016213854A1 (en) 2016-09-01
US9139836B2 (en) 2015-09-22
US20120174242A1 (en) 2012-07-05
US20140227759A1 (en) 2014-08-14
SG189496A1 (en) 2013-05-31
IL225867A0 (en) 2013-07-31
CN103370319A (zh) 2013-10-23
AU2011323418A1 (en) 2013-05-30
US9840730B2 (en) 2017-12-12
SG190104A1 (en) 2013-06-28
ES2567410T3 (es) 2016-04-22
US20240158831A1 (en) 2024-05-16
SG10201607774RA (en) 2016-11-29
CN103443121B (zh) 2016-04-20
US10774364B2 (en) 2020-09-15
WO2012061530A3 (en) 2012-09-27
US20140223590A1 (en) 2014-08-07
EP3498709A1 (en) 2019-06-19
CA3081115A1 (en) 2012-05-10
IL225867B (en) 2018-07-31
JP2016145219A (ja) 2016-08-12
IL258790A (en) 2018-06-28
CA2817102C (en) 2020-07-28
PL2635595T4 (pl) 2019-04-30
KR101538624B1 (ko) 2015-07-22
JP6374420B2 (ja) 2018-08-15
IL288070A (en) 2022-01-01
CN103370319B (zh) 2016-08-24
JP2016144454A (ja) 2016-08-12
CA2815227C (en) 2019-03-26
WO2012061530A2 (en) 2012-05-10
US20200399676A1 (en) 2020-12-24
EP3498710A1 (en) 2019-06-19
IL258790B (en) 2020-05-31
JP2014500011A (ja) 2014-01-09
BR112013010855A2 (pt) 2017-06-27
DK2635595T3 (en) 2019-03-04
US8669103B2 (en) 2014-03-11
IL274316B (en) 2021-12-01
DK2635582T3 (da) 2016-03-14
JP2018127459A (ja) 2018-08-16
CN106148362A (zh) 2016-11-23
PL2635582T3 (pl) 2016-08-31
WO2012061529A1 (en) 2012-05-10
IL288070B2 (en) 2023-02-01
BR112013010487A2 (pt) 2016-11-22
US20180327810A1 (en) 2018-11-15
US9951373B2 (en) 2018-04-24
CN103443121A (zh) 2013-12-11
CA2817102A1 (en) 2012-05-10
PT2635595T (pt) 2019-01-29
JP6155424B2 (ja) 2017-07-05
CA2815227A1 (en) 2012-05-10
KR101939553B1 (ko) 2019-01-17
BR112013010487B1 (pt) 2021-02-02
SG10202103336SA (en) 2021-04-29
JP6539689B2 (ja) 2019-07-03
JP2014503485A (ja) 2014-02-13
EP2635582A1 (en) 2013-09-11
IL288070B (en) 2022-10-01
AU2016213854B2 (en) 2018-01-04
US8809529B2 (en) 2014-08-19
IL274316A (en) 2020-06-30
JP6752839B2 (ja) 2020-09-09
US9938564B2 (en) 2018-04-10
US20120117667A1 (en) 2012-05-10
IL226114A0 (en) 2013-06-27
US12215375B2 (en) 2025-02-04
US11661623B2 (en) 2023-05-30
US20150064731A1 (en) 2015-03-05
US20160002703A1 (en) 2016-01-07
AU2011323419A1 (en) 2013-05-30
US12435354B2 (en) 2025-10-07
KR20130099997A (ko) 2013-09-06
JP2017176182A (ja) 2017-10-05
AU2011323418B2 (en) 2017-07-27
JP6574721B2 (ja) 2019-09-11
CN106148362B (zh) 2021-04-30

Similar Documents

Publication Publication Date Title
PL2635595T3 (pl) Lucyferazy otrzymane z krewetki oplophorus, nowe substraty koelenterazyny oraz sposoby stosowania
IL250824B (en) Certain amino-pyrimidines, their assemblies, and methods of using them
AP2011005943A0 (en) Atropisomers of 2-purinyl-3-tolylquinazolinone derivatives and methods of use.
ME03766B (me) Određeni amino-pirimidini, njihove kompozicije, i metode njihove upotrebe
PL2552959T3 (pl) Przeciwciała przeciwko MUC16 i metody stosowania
BR112012017441A2 (pt) compostos e métodos
IL230662A0 (en) Indazoles
BR112013008905A2 (pt) mecanismo de dosagem e método de dosagem
IL225869A (en) Colentrazine derivatives and methods for their use
IT1400121B1 (it) Copoliestere alifatico-aromatico e sue miscele.
FI20105572A0 (fi) Muokattu beeta-laktamaasi ja siihen liittyvät menetelmät ja käytöt
PT3650125T (pt) Atomizador de líquidos e métodos
HRP20160922T1 (hr) Oligopeptidni spojevi i njihove uporabe
IL250606A0 (en) D2 antagonists, methods of synthesis and methods of use
BR112013002544A2 (pt) seção de armario e armario
IL225785A0 (en) Heterocyclic compounds, compositions comprising the same and uses thereof
EP2742033A4 (en) FLAVONOID COMPOUNDS AND METHODS OF USE
EP2534954A4 (en) COOKING DEVICE, SMOKING AND AROMATISATION
HRP20170225T1 (hr) Fosfoplatini i njihova uporaba za liječenje karcinoma
ZA201308268B (en) Herbicidal composition comprising pinoxaden and fluroxypyrester, and methods of use thereof
HRP20182083T1 (hr) Nova kombinacija i uporaba
DK2758074T3 (da) Bakteriestammer der udtrykker methylasegener og anvendelser deraf
HRP20181744T1 (hr) Komplet za pronalaženje, praćenje i određivanje gvhd
PT2580226T (pt) Derivados de trissacárido e sua utilização como adjuvantes
ES1071902Y (es) Mecanismo de termosoldadura y precorte