[go: up one dir, main page]

PL2635595T4 - Lucyferazy otrzymane z krewetki oplophorus, nowe substraty koelenterazyny oraz sposoby stosowania - Google Patents

Lucyferazy otrzymane z krewetki oplophorus, nowe substraty koelenterazyny oraz sposoby stosowania

Info

Publication number
PL2635595T4
PL2635595T4 PL11785534T PL11785534T PL2635595T4 PL 2635595 T4 PL2635595 T4 PL 2635595T4 PL 11785534 T PL11785534 T PL 11785534T PL 11785534 T PL11785534 T PL 11785534T PL 2635595 T4 PL2635595 T4 PL 2635595T4
Authority
PL
Poland
Prior art keywords
oplophorus
methods
novel coelenterazine
derived luciferases
coelenterazine substrates
Prior art date
Application number
PL11785534T
Other languages
English (en)
Other versions
PL2635595T3 (pl
Inventor
Brock Binkowski
Lance P. Encell
Mary Hall
Matthew B. Robers
Michael R. Slater
Keith V. Wood
Monika G. Wood
Dieter Klaubert
Poncho Meisenheimer
James Unch
Michael P. Valley
Original Assignee
Promega Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44983728&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PL2635595(T4) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Promega Corporation filed Critical Promega Corporation
Publication of PL2635595T4 publication Critical patent/PL2635595T4/pl
Publication of PL2635595T3 publication Critical patent/PL2635595T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12005—Renilla-luciferin 2-monooxygenase (1.13.12.5), i.e. renilla-luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12013—Oplophorus-luciferin 2-monooxygenase (1.13.12.13)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
PL11785534T 2010-11-02 2011-11-02 Lucyferazy otrzymane z krewetki oplophorus, nowe substraty koelenterazyny oraz sposoby stosowania PL2635595T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US40942210P 2010-11-02 2010-11-02
PCT/US2011/059018 WO2012061530A2 (en) 2010-11-02 2011-11-02 Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
EP11785534.6A EP2635595B1 (en) 2010-11-02 2011-11-02 Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use

Publications (2)

Publication Number Publication Date
PL2635595T4 true PL2635595T4 (pl) 2019-04-30
PL2635595T3 PL2635595T3 (pl) 2019-04-30

Family

ID=44983728

Family Applications (2)

Application Number Title Priority Date Filing Date
PL11785534T PL2635595T3 (pl) 2010-11-02 2011-11-02 Lucyferazy otrzymane z krewetki oplophorus, nowe substraty koelenterazyny oraz sposoby stosowania
PL11782732T PL2635582T3 (pl) 2010-11-02 2011-11-02 Nowe substraty będące koelenterazynami i sposoby zastosowania

Family Applications After (1)

Application Number Title Priority Date Filing Date
PL11782732T PL2635582T3 (pl) 2010-11-02 2011-11-02 Nowe substraty będące koelenterazynami i sposoby zastosowania

Country Status (15)

Country Link
US (10) US8669103B2 (pl)
EP (4) EP2635595B1 (pl)
JP (6) JP5889910B2 (pl)
KR (2) KR101538624B1 (pl)
CN (3) CN103370319B (pl)
AU (2) AU2011323418B2 (pl)
BR (2) BR112013010487B1 (pl)
CA (3) CA2817102C (pl)
DK (2) DK2635582T3 (pl)
ES (2) ES2567410T3 (pl)
IL (5) IL225867B (pl)
PL (2) PL2635595T3 (pl)
PT (1) PT2635595T (pl)
SG (5) SG10202103336SA (pl)
WO (2) WO2012061529A1 (pl)

Families Citing this family (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8183036B2 (en) 2003-10-10 2012-05-22 Promega Corporation Luciferase biosensor
EP2327768B1 (en) 2006-04-03 2015-09-09 Promega Corporation Permuted and nonpermuted luciferase biosensors
JP6110591B2 (ja) 2008-05-19 2017-04-05 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ ｃＡＭＰのためのルシフェラーゼバイオセンサー
EP3744834B1 (en) * 2009-05-01 2025-09-10 Promega Corporation Synthetic oplophorus luciferases with enhanced light output
US9290794B2 (en) * 2010-05-11 2016-03-22 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
EP2990479B1 (en) 2010-05-11 2019-03-27 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
DK2635583T3 (en) 2010-11-02 2015-08-03 Promega Corp Coelenterazinderivater and methods of use thereof
SG10202103336SA (en) * 2010-11-02 2021-04-29 Promega Corp Novel coelenterazine substrates and methods of use
JP6522339B2 (ja) 2011-11-21 2019-05-29 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ カルボキシｘローダミン類似体
CA3174484A1 (en) 2012-12-12 2014-06-19 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
EP2931305B1 (en) * 2012-12-12 2018-11-28 Promega Corporation Compositions and methods for capture of cellular targets of bioactive agents
US10067149B2 (en) 2012-12-12 2018-09-04 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
JP6511720B2 (ja) 2013-02-28 2019-05-15 Jnc株式会社 コドン最適化変異エビルシフェラーゼ遺伝子およびその使用方法
BR112015023394B8 (pt) * 2013-03-15 2023-09-26 Promega Corp Sistema e complexo bioluminescente
US11072812B2 (en) 2013-03-15 2021-07-27 Promega Corporation Substrates for covalent tethering of proteins to functional groups or solid surfaces
US10168323B2 (en) 2013-03-15 2019-01-01 Promega Corporation Compositions and methods for capture of cellular targets of bioactive agents
CN105392892A (zh) * 2013-03-27 2016-03-09 六品科技公司 重组噬菌体和细菌检测方法
JP6040846B2 (ja) * 2013-04-08 2016-12-07 Jnc株式会社 セレンテラジン類縁体
CN105531382A (zh) * 2013-06-19 2016-04-27 六品科技公司 基于噬菌体的细菌检测法
WO2015006743A1 (en) 2013-07-12 2015-01-15 The Children's Hospital Of Philadelphia Aav vector and assay for anti-aav (adeno-associated virus) neutralizing antibodies
EP3022316B1 (en) 2013-07-18 2017-11-29 Ecole Polytechnique Fédérale de Lausanne (EPFL) Means and methods for bioluminescence resonance energy transfer (bret) analysis in a biological sample
JP6484987B2 (ja) 2013-10-21 2019-03-20 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
WO2015067302A1 (en) 2013-11-05 2015-05-14 Ecole Polytechnique Federale De Lausanne (Epfl) Sensor molecules and uses thereof
JP6442825B2 (ja) 2013-12-26 2018-12-26 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
WO2015116867A1 (en) 2014-01-29 2015-08-06 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements
JP6588917B2 (ja) 2014-01-29 2019-10-09 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 生細胞への適用のためのプロ基質
JP6256118B2 (ja) * 2014-03-11 2018-01-10 Jnc株式会社 エビルシフェラーゼの触媒蛋白質のキメラ遺伝子及びその使用法
JP6265020B2 (ja) 2014-04-16 2018-01-24 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
BR112016027504B1 (pt) * 2014-05-23 2023-04-18 Promega Corporation Composição e método para estabilizar um substrato luminogênico e kit que compreende a referida composição
WO2016040788A1 (en) 2014-09-11 2016-03-17 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
JP6814132B2 (ja) * 2014-09-12 2021-01-13 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 内部タンパク質タグ
US11365402B2 (en) * 2014-09-12 2022-06-21 Promega Corporation Internal protein tags
US20190054117A1 (en) * 2014-12-19 2019-02-21 Novartis Ag Dimerization switches and uses thereof
EP4455668A3 (en) 2015-02-05 2025-02-19 Promega Corporation Luciferase-based thermal shift assays
EP3304078B1 (en) 2015-06-05 2022-08-31 Promega Corporation Cell-permeable, cell-compatible, and cleavable linkers for covalent tethering of functional elements
CN107922428B (zh) 2015-06-25 2021-02-05 普洛麦格公司 噻吩并吡咯化合物及其作为刺虾源性荧光素酶的抑制剂的用途
US20170029798A1 (en) * 2015-07-27 2017-02-02 Cellivery Therapeutics, Inc. Development of Improved Cell-Permeable (iCP) Parkin Recombinant Protein as a Protein-Based Anti-Neurodegenerative Agent for the Treatment of Parkinson's Disease-Associated Phenotypes by Utilizing BBB-Penetrating Protein Delivery System MITT, Enabled by Advanced Macromolecule Transduction Domain (aMTD)
EP4086261B1 (en) 2016-02-15 2025-09-03 Promega Corporation Process for the preparation of coelenterazine analogues
EP3428277B1 (en) 2016-03-11 2023-05-10 National Institute of Advanced Industrial Science and Technology Light-emitting enzyme protein
US10634680B2 (en) 2016-04-26 2020-04-28 University Of Utah Research Foundation Target-binding activated split reporter systems for analyte detection and related components and methods
CN105968114B (zh) * 2016-05-27 2018-11-09 山东大学 一种腔肠素类似物及其制备方法与应用
US10000500B2 (en) 2016-07-28 2018-06-19 Promega Corporation Coelenterazine analogues
US10202584B2 (en) * 2016-09-01 2019-02-12 The Regents Of The University Of California Red-shifted luciferase-luciferin pairs for enhanced bioluminescence
EP3510035B1 (en) 2016-09-09 2021-04-07 Promega Corporation Dual protected pro-coelenterazine substrates
JP7200102B2 (ja) 2016-11-01 2023-01-06 プロメガ コーポレイション エネルギー受容体に係留されたセレンテラジン類縁体
EP4279490A3 (en) 2016-12-01 2024-04-10 Promega Corporation Cell impermeable coelenterazine analogues
WO2018102726A1 (en) * 2016-12-01 2018-06-07 Promega Corporation 5,5-disubstituted luciferins and their use in luciferase-based assays
JP7161475B2 (ja) 2016-12-28 2022-10-26 プロメガ コーポレイション 官能化ｎａｎｏｌｕｃ阻害剤
EP3630994B1 (en) 2017-05-30 2022-08-10 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Sensors, methods and kits for detecting nadph based on resonance energy transfer.
WO2019010094A1 (en) * 2017-07-06 2019-01-10 Promega Corporation STERILE SERIES PRO-COELENTERAZINE ANALOGS
CN108107209B (zh) * 2017-09-19 2020-07-24 中国中医科学院医学实验中心 一种检测分泌型报告蛋白含量的优化方法及其所用生物材料
WO2019213119A1 (en) 2018-05-01 2019-11-07 Promega Corporation Coelenterazine compounds as nanoluc suicide substrates
JP7432529B2 (ja) 2018-05-30 2024-02-16 プロメガ コーポレイション 広域スペクトルキナーゼ結合剤
WO2019232384A1 (en) 2018-06-01 2019-12-05 Promega Corporation Inhibitors of oplophorus luciferase-derived bioluminescent complexes
CN112567028A (zh) * 2018-06-12 2021-03-26 普洛麦格公司 多部分萤光素酶
US10962538B2 (en) 2018-06-14 2021-03-30 The Trustees Of Columbia University In The City Of New York Assays using arrestin recruitment and unmodified receptors
WO2020023871A1 (en) 2018-07-27 2020-01-30 Promega Corporation Quinone-containing conjugates
US11691976B2 (en) 2018-08-23 2023-07-04 Promega Corporation Coelenterazine analogues
US11534504B2 (en) 2018-10-03 2022-12-27 Promega Corporation Compositions and methods for stabilizing coelenterazine and analogs and derivatives thereof
WO2020113036A2 (en) 2018-11-28 2020-06-04 Promega Corporation Reactive peptide labeling
WO2020117954A2 (en) 2018-12-04 2020-06-11 Promega Corporation Broad spectrum gpcr binding agents
WO2020176755A1 (en) * 2019-02-27 2020-09-03 University Of Virginia Patent Foundation Atp-independent bioluminescent reporter variants to improve in vivo imaging
WO2020191339A1 (en) 2019-03-20 2020-09-24 Promega Corporation Photoaffinity probes
US11360096B2 (en) 2019-05-16 2022-06-14 Duke University Complex BRET technique for measuring biological interactions
EP3986956A1 (en) 2019-06-24 2022-04-27 Promega Corporation Modified polyamine polymers for delivery of biomolecules into cells
AU2020392245A1 (en) 2019-11-27 2022-06-23 Promega Corporation Multipartite luciferase peptides and polypeptides
EP4073512B1 (en) 2019-12-10 2025-11-12 Promega Corporation Compositions and methods for bioluminescent detection using multifunctional probes
EP3882632A1 (en) 2020-03-18 2021-09-22 Universite De Bordeaux Novel ion conducting channel fusion subunits and methods of use thereof
CN113527231A (zh) * 2020-04-15 2021-10-22 深圳华大生命科学研究院 杂环取代腔肠素类化合物的合成方法
EP3904508A1 (en) 2020-04-27 2021-11-03 Institut Pasteur Luciferase linked immunosorbent assay
WO2021237118A2 (en) 2020-05-22 2021-11-25 Promega Corporation Enhancement of kinase target engagement
EP4204808A1 (en) 2020-08-28 2023-07-05 Promega Corporation Target engagement assay for ras proteins
KR20230124938A (ko) 2020-12-25 2023-08-28 추가이 세이야쿠 가부시키가이샤 복수의 표적 분자와 함께 복합체를 형성할 수 있는후보 분자의 스크리닝 방법
CN118703457A (zh) * 2021-04-01 2024-09-27 深圳华大生命科学研究院 一种刺虾荧光素酶Nluc的突变体及其应用
EP4392460A1 (en) * 2021-08-24 2024-07-03 Institut Pasteur Luminescent based antigen assay
EP4519425A1 (en) 2022-05-04 2025-03-12 Promega Corporation Modified dehalogenase with extended surface loop regions
US20230357266A1 (en) 2022-05-04 2023-11-09 Promega Corporation Photoactivatable compounds and uses thereof
US20240060059A1 (en) 2022-05-04 2024-02-22 Promega Corporation Circularly permuted dehalogenase variants
US20240174992A1 (en) 2022-05-04 2024-05-30 Promega Corporation Split modified dehalogenase variants
CN115349611B (zh) * 2022-08-05 2023-10-24 江南大学 一种改善南美白对虾虾糜凝胶品质的方法
CN116068198B (zh) * 2022-11-30 2024-01-09 深圳湾实验室 Ppi原位检测方法及其载体、诊断试剂、试剂盒和应用
US20240377404A1 (en) * 2023-03-24 2024-11-14 Promega Corporation Bioluminescence-triggered photocatalytic activation
JP7482563B1 (ja) 2023-06-21 2024-05-14 国立大学法人大阪大学 シイノトモシビタケ由来の発光関連遺伝子
DE102023120682B3 (de) 2023-08-03 2024-10-24 Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut Zytotoxizitätstest zum Nachweis von Zellschäden
GB202404021D0 (en) 2024-03-20 2024-05-01 Ipsen Biopharm Ltd Cell-based neurotoxin assay

Family Cites Families (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3477549D1 (en) 1983-08-17 1989-05-11 Allied Signal Inc Gas generator and method of generating gas
JPH0515398A (ja) 1991-07-15 1993-01-26 Nippon Oil & Fats Co Ltd オキシダーゼ酵素の定量方法
US5368484A (en) 1992-05-22 1994-11-29 Atari Games Corp. Vehicle simulator with realistic operating feedback
US6387675B1 (en) 1994-01-03 2002-05-14 Promega Corporation Mutant luciferases
WO1995018853A1 (en) 1994-01-03 1995-07-13 Promega Corporation Mutant luciferases
GB9501170D0 (en) 1994-03-23 1995-03-08 Secr Defence Luciferases
JP3648763B2 (ja) 1994-08-23 2005-05-18 日本油脂株式会社 ウミホタルルシフェリン誘導体および糖加水分解酵素の定量方法
GB9417593D0 (en) 1994-09-01 1994-10-19 Secr Defence Luciferase labelling method
US5670356A (en) 1994-12-12 1997-09-23 Promega Corporation Modified luciferase
DK0804587T3 (da) 1995-01-20 2004-10-11 Secr Defence Mutantluciferaser
JPH08294397A (ja) 1995-04-27 1996-11-12 Nippon Oil & Fats Co Ltd 免疫学的活性物質の測定方法
US5998204A (en) 1997-03-14 1999-12-07 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
GB9707486D0 (en) 1997-04-11 1997-05-28 Secr Defence Enzyme assays
CA2285571C (en) 1997-04-16 2008-12-02 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Immunomodulatory molecules and process for producing the same
EP1015601B1 (en) 1997-09-19 2015-01-07 Promega Corporation Thermostable luciferases and methods of production
US6602677B1 (en) 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
GB9823468D0 (en) 1998-10-28 1998-12-23 Secr Defence Novel enzyme
JP3694181B2 (ja) 1999-01-05 2005-09-14 キッコーマン株式会社 変異型ルシフェラーゼ、変異型ルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び変異型ルシフェラーゼの製造法
GB9925161D0 (en) 1999-10-26 1999-12-22 Secr Defence Novel enzyme
WO2001031028A2 (en) 1999-10-26 2001-05-03 The Secretary Of State For Defence Mutant luciferase
US20010046687A1 (en) 2000-03-31 2001-11-29 Dicesare Joseph L. Method for the enhancement of luminescence intensity from a reaction between adenosine triphosphate (ATP) and a luciferase/luciferin reactant system
JP4613441B2 (ja) * 2000-04-26 2011-01-19 チッソ株式会社 新規ルシフェラーゼおよび発光蛋白質
DE60142761D1 (de) 2000-04-26 2010-09-23 Chisso Corp Oplophorus Luciferase
US7118878B1 (en) 2000-06-09 2006-10-10 Promega Corporation Method for increasing luminescence assay sensitivity
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
US7268229B2 (en) 2001-11-02 2007-09-11 Promega Corporation Compounds to co-localize luminophores with luminescent proteins
JP4451663B2 (ja) 2002-02-01 2010-04-14 プロメガ コーポレイション 生物発光プロテアーゼ分析方法
JP2006508339A (ja) 2002-09-20 2006-03-09 プロメガ　コーポレイション シトクロムｐ４５０活性を測定するための発光を利用する方法およびプローブ
DK1413584T3 (da) * 2002-10-21 2005-07-04 Axxam S R L Fotoprotein med forbedret bioluminescens
EP2325328B1 (en) 2002-12-23 2014-02-12 Promega Corporation Improved luciferase-based assays
CA2514564A1 (en) 2003-01-31 2005-07-26 Promega Corporation Covalent tethering of functional groups to proteins
US7429472B2 (en) 2003-01-31 2008-09-30 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
US20040224377A1 (en) 2003-02-12 2004-11-11 Erika Hawkins Compositions and methods to quench light from optical reactions
US20040178545A1 (en) 2003-03-14 2004-09-16 Cates Larry E. System for optically analyzing a molten metal bath
US8183036B2 (en) 2003-10-10 2012-05-22 Promega Corporation Luciferase biosensor
JP2007518727A (ja) 2004-01-08 2007-07-12 ウニヴェルジテート・チューリッヒ 配位子としてビタミンｂ１２を有する金属錯体
US7553632B2 (en) 2004-01-22 2009-06-30 Promega Corporation Luminogenic and nonluminogenic multiplex assay
US7416854B2 (en) 2004-01-22 2008-08-26 Promega Corporation Luminogenic and nonluminogenic multiplex assay
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
EP2395358B1 (en) 2004-07-30 2017-03-29 Promega Corporation Covalent tethering of functional groups to proteins
US20070087400A1 (en) 2004-07-30 2007-04-19 Aldis Darzins Covalent tethering of functional groups to proteins and substrates therefor
US7728118B2 (en) 2004-09-17 2010-06-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
JP4639904B2 (ja) 2005-03-30 2011-02-23 チッソ株式会社 蛍光活性を有するルシフェラーゼの活性増強方法
JP3844082B2 (ja) * 2005-04-08 2006-11-08 キッコーマン株式会社 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び耐熱性ホタルルシフェラーゼの製造法
EP2778234B1 (en) 2005-05-31 2017-09-27 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
EP1935986B1 (en) 2005-05-31 2014-04-16 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
US7495579B2 (en) 2005-06-13 2009-02-24 Sirota J Marcos Traffic light status remote sensor system
CN101287842A (zh) 2005-08-17 2008-10-15 耶达研究及发展有限公司 用于测定生物活性血清对氧磷酶水平的方法和组合物
JP4999341B2 (ja) * 2005-09-06 2012-08-15 株式会社バイオエネックス 変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法
EP2327768B1 (en) 2006-04-03 2015-09-09 Promega Corporation Permuted and nonpermuted luciferase biosensors
JP2007306871A (ja) 2006-05-19 2007-11-29 Chisso Corp 封入体可溶化方法及び精製方法
US8420367B2 (en) 2006-10-30 2013-04-16 Promega Corporation Polynucleotides encoding mutant hydrolase proteins with enhanced kinetics and functional expression
WO2008086035A2 (en) 2007-01-10 2008-07-17 Promega Corporation Split mutant hydrolase fusion reporter and uses thereof
US20080248511A1 (en) 2007-03-26 2008-10-09 Promega Corporation Methods to quench light from optical reactions
CN101849005A (zh) 2007-11-05 2010-09-29 普罗梅加公司 杂合融合报道子及其应用
JP6110591B2 (ja) 2008-05-19 2017-04-05 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ ｃＡＭＰのためのルシフェラーゼバイオセンサー
CN102159948B (zh) 2008-07-22 2014-06-25 普罗美加公司 基于adp检测的发光磷酸转移酶或atp水解酶测定
TWI375901B (en) 2008-08-05 2012-11-01 Elan Microelectronics Corp Touch screen and method for positioning coordinate
US8546568B2 (en) * 2009-02-09 2013-10-01 Jnc Corporation Coelenterazine analogs and manufacturing method thereof
JPWO2010119721A1 (ja) * 2009-04-17 2012-10-22 独立行政法人産業技術総合研究所 超高輝度で安定な人工生物発光酵素
EP3744834B1 (en) 2009-05-01 2025-09-10 Promega Corporation Synthetic oplophorus luciferases with enhanced light output
WO2011038219A1 (en) 2009-09-24 2011-03-31 Promega Corporation Proteases having improved stability, solubility and activity
JP5605727B2 (ja) * 2010-04-06 2014-10-15 Ｊｎｃ株式会社 セレンテラジン類縁体及びセレンテラミド類縁体
EP2990479B1 (en) 2010-05-11 2019-03-27 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
WO2012030960A1 (en) 2010-09-01 2012-03-08 Promega Corporation Oxidized glutathione assay
US9284188B2 (en) 2010-09-03 2016-03-15 The Regents Of The University Of California Cytosolic delivery of materials with endosome-disrupting colloids
DK2635583T3 (en) 2010-11-02 2015-08-03 Promega Corp Coelenterazinderivater and methods of use thereof
SG10202103336SA (en) * 2010-11-02 2021-04-29 Promega Corp Novel coelenterazine substrates and methods of use
EP2751089B1 (en) 2011-09-02 2018-11-14 Promega Corporation Compounds and methods for assaying redox state of metabolically active cells and methods for measuring nad(p)/nad(p)h

Also Published As

Publication number Publication date
IL288070B2 (en) 2023-02-01
US9951373B2 (en) 2018-04-24
US20120117667A1 (en) 2012-05-10
BR112013010855A2 (pt) 2017-06-27
IL258790B (en) 2020-05-31
SG10201607774RA (en) 2016-11-29
JP5889910B2 (ja) 2016-03-22
US20220348983A1 (en) 2022-11-03
PL2635582T3 (pl) 2016-08-31
CN103443121A (zh) 2013-12-11
JP2014500011A (ja) 2014-01-09
US8669103B2 (en) 2014-03-11
US10774364B2 (en) 2020-09-15
EP2635582B1 (en) 2016-01-27
JP2016144454A (ja) 2016-08-12
US9139836B2 (en) 2015-09-22
SG190104A1 (en) 2013-06-28
US20120174242A1 (en) 2012-07-05
CN106148362A (zh) 2016-11-23
CN103443121B (zh) 2016-04-20
JP6574721B2 (ja) 2019-09-11
WO2012061530A8 (en) 2013-06-06
AU2016213854B2 (en) 2018-01-04
EP2635595A2 (en) 2013-09-11
ES2723773T3 (es) 2019-09-02
US9840730B2 (en) 2017-12-12
CA2815227C (en) 2019-03-26
WO2012061529A1 (en) 2012-05-10
IL274316A (en) 2020-06-30
US20140223590A1 (en) 2014-08-07
US20150064731A1 (en) 2015-03-05
AU2016213854A1 (en) 2016-09-01
PL2635595T3 (pl) 2019-04-30
BR112013010487B1 (pt) 2021-02-02
JP2018127459A (ja) 2018-08-16
SG10202103336SA (en) 2021-04-29
PT2635595T (pt) 2019-01-29
AU2011323418B2 (en) 2017-07-27
EP2635582A1 (en) 2013-09-11
AU2011323418A1 (en) 2013-05-30
CA2817102A1 (en) 2012-05-10
WO2012061530A3 (en) 2012-09-27
JP2017176182A (ja) 2017-10-05
US8809529B2 (en) 2014-08-19
US20160002703A1 (en) 2016-01-07
SG10201608550SA (en) 2016-12-29
EP2635595B1 (en) 2019-01-09
CA2817102C (en) 2020-07-28
US9938564B2 (en) 2018-04-10
US12435354B2 (en) 2025-10-07
CN103370319B (zh) 2016-08-24
CN106148362B (zh) 2021-04-30
US20200399676A1 (en) 2020-12-24
CA3081115A1 (en) 2012-05-10
US11661623B2 (en) 2023-05-30
US20140227759A1 (en) 2014-08-14
IL274316B (en) 2021-12-01
CN103370319A (zh) 2013-10-23
JP6374420B2 (ja) 2018-08-15
US20180327810A1 (en) 2018-11-15
IL288070A (en) 2022-01-01
BR112013010487A2 (pt) 2016-11-22
CA2815227A1 (en) 2012-05-10
IL288070B (en) 2022-10-01
IL226114A0 (en) 2013-06-27
IL225867B (en) 2018-07-31
KR101939553B1 (ko) 2019-01-17
US20240158831A1 (en) 2024-05-16
JP6539689B2 (ja) 2019-07-03
JP2014503485A (ja) 2014-02-13
JP2016145219A (ja) 2016-08-12
DK2635582T3 (da) 2016-03-14
DK2635595T3 (en) 2019-03-04
KR20130084312A (ko) 2013-07-24
JP6155424B2 (ja) 2017-07-05
KR101538624B1 (ko) 2015-07-22
IL258790A (en) 2018-06-28
JP6752839B2 (ja) 2020-09-09
KR20130099997A (ko) 2013-09-06
ES2567410T3 (es) 2016-04-22
SG189496A1 (en) 2013-05-31
EP3498710A1 (en) 2019-06-19
WO2012061530A2 (en) 2012-05-10
EP3498709A1 (en) 2019-06-19
IL226114B (en) 2018-04-30
IL225867A0 (en) 2013-07-31
US12215375B2 (en) 2025-02-04
AU2011323419A1 (en) 2013-05-30

Similar Documents

Publication Publication Date Title
IL288070A (en) Luciferases derived from Eulophorus, new coelantrazine materials and methods of use
IL250824B (en) Certain amino-pyrimidines, their assemblies, and methods of using them
IL225869A0 (en) Colantrazin derivatives and methods of using them
IL214331A0 (en) Methods of reducing the proliferation and viability of microbial agents
ZA201300612B (en) Novel bacteria and methods of use thereof
SMT202200371T1 (it) Determinate triazolopirazine, loro composizioni e loro metodi d'uso
ZA201209205B (en) Pyridone and aza-pyridone compounds and methods of use
ZA201208578B (en) Composition,method and use
EP2663635A4 (en) MICROBIAL COMPOSITIONS AND METHOD THEREFOR
EP2536283B8 (en) Phenyl-heteroaryl derivatives and methods of use thereof
ZA201200226B (en) Opsin-binding ligands, compositions and methods of use
ZA201208948B (en) Modified beta-lactamases and methods and uses related thereto
ZA201308268B (en) Herbicidal composition comprising pinoxaden and fluroxypyrester, and methods of use thereof
IL207207A0 (en) Colorant compositions and production methods therefor
GB201019361D0 (en) Biosensor and uses thereof
GB201118217D0 (en) Carrier, kit and method of use
GB201115285D0 (en) Biosensor and uses thereof
HK1188445A (en) Hepatitis c inhibitors and uses thereof
HK1177747A (en) Hsa-related compositions and methods of use
HK1177104A (en) New forms of rifaximin and uses thereof
HK1188612A (en) Enzymes and uses thereof