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GB2450454A - Terminating substrates for DNA polymerases - Google Patents

Terminating substrates for DNA polymerases Download PDF

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Publication number
GB2450454A
GB2450454A GB0818768A GB0818768A GB2450454A GB 2450454 A GB2450454 A GB 2450454A GB 0818768 A GB0818768 A GB 0818768A GB 0818768 A GB0818768 A GB 0818768A GB 2450454 A GB2450454 A GB 2450454A
Authority
GB
United Kingdom
Prior art keywords
dna polymerases
substrates
terminating
terminating substrates
invention concerns
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB0818768A
Other versions
GB0818768D0 (en
GB2450454B (en
Inventor
Jari Hovinen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wallac Oy
Original Assignee
Wallac Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wallac Oy filed Critical Wallac Oy
Publication of GB0818768D0 publication Critical patent/GB0818768D0/en
Publication of GB2450454A publication Critical patent/GB2450454A/en
Application granted granted Critical
Publication of GB2450454B publication Critical patent/GB2450454B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/6558—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system
    • C07F9/65586—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system at least one of the hetero rings does not contain nitrogen as ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/6558—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system
    • C07F9/65583—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system each of the hetero rings containing nitrogen as ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/6561—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing systems of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring or ring system, with or without other non-condensed hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H23/00—Compounds containing boron, silicon or a metal, e.g. chelates or vitamin B12
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

This invention concerns novel terminating substrates for DNA polymerases. The substrates are nucleoside triphosphates labeled with lanthanide chelates.
GB0818768A 2006-03-13 2007-03-08 Terminating substrates for DNA polymerases Expired - Fee Related GB2450454B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20065162A FI20065162A0 (en) 2006-03-13 2006-03-13 Terminating substrates for DNA polymerases
PCT/FI2007/050127 WO2007104834A1 (en) 2006-03-13 2007-03-08 Terminating substrates for dna polymerases

Publications (3)

Publication Number Publication Date
GB0818768D0 GB0818768D0 (en) 2008-11-19
GB2450454A true GB2450454A (en) 2008-12-24
GB2450454B GB2450454B (en) 2011-04-13

Family

ID=36192007

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0818768A Expired - Fee Related GB2450454B (en) 2006-03-13 2007-03-08 Terminating substrates for DNA polymerases

Country Status (3)

Country Link
FI (1) FI20065162A0 (en)
GB (1) GB2450454B (en)
WO (1) WO2007104834A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100041798A (en) 2007-06-29 2010-04-22 한국화학연구원 Novel hiv reverse transcriptase inhibitors
BRPI0813269A2 (en) 2007-06-29 2014-12-30 Korean Res Inst Of Chemical Technology Reverse HIV TRANSCRIPTASE INHIBITORS
US8119800B2 (en) 2007-12-21 2012-02-21 Korea Research Institute Of Chemical Technology Processes for preparing HIV reverse transcriptase inhibitors
CR20170420A (en) 2015-03-13 2017-10-03 Forma Therapeutics Inc ALFA-CINAMIDE COMPOUNDS AND COMPOSITIONS AS HDAC8 INHIBITORS

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0251786A2 (en) * 1986-07-02 1988-01-07 E.I. Du Pont De Nemours And Company Alkynylamino-nucleotides
EP0340675A2 (en) * 1988-05-02 1989-11-08 The Perkin-Elmer Corporation Time-resolved fluorimetric detection of lanthanide labeled nucleotides
WO1990000623A1 (en) * 1988-07-08 1990-01-25 Wallac Oy Multi-label time-resolved fluorescence analysis of nucleic acid sequences using lanthanide chelates
US5798210A (en) * 1993-03-26 1998-08-25 Institut Pasteur Derivatives utilizable in nucleic acid sequencing
US6255475B1 (en) * 1995-01-31 2001-07-03 Marek Kwiatkowski Chain terminators, the use thereof for nucleic acid sequencing and synthesis and a method of their preparation
US20050084451A1 (en) * 2003-08-29 2005-04-21 Wallac Oy Novel chelating agents and chelates and their use
US20050181393A1 (en) * 2003-12-18 2005-08-18 Wallac Oy Novel chelating agents and highly luminescent and stable chelates and their use

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0251786A2 (en) * 1986-07-02 1988-01-07 E.I. Du Pont De Nemours And Company Alkynylamino-nucleotides
EP0340675A2 (en) * 1988-05-02 1989-11-08 The Perkin-Elmer Corporation Time-resolved fluorimetric detection of lanthanide labeled nucleotides
WO1990000623A1 (en) * 1988-07-08 1990-01-25 Wallac Oy Multi-label time-resolved fluorescence analysis of nucleic acid sequences using lanthanide chelates
US5798210A (en) * 1993-03-26 1998-08-25 Institut Pasteur Derivatives utilizable in nucleic acid sequencing
US6255475B1 (en) * 1995-01-31 2001-07-03 Marek Kwiatkowski Chain terminators, the use thereof for nucleic acid sequencing and synthesis and a method of their preparation
US20050084451A1 (en) * 2003-08-29 2005-04-21 Wallac Oy Novel chelating agents and chelates and their use
US20050181393A1 (en) * 2003-12-18 2005-08-18 Wallac Oy Novel chelating agents and highly luminescent and stable chelates and their use

Also Published As

Publication number Publication date
GB0818768D0 (en) 2008-11-19
GB2450454B (en) 2011-04-13
WO2007104834A1 (en) 2007-09-20
FI20065162A0 (en) 2006-03-13

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20200308