FR3003167A1 - COSMETIC OR DERMATOLOGICAL COMPOSITION AND USE THEREOF - Google Patents

Abstract

The present invention relates to a cosmetic or dermatological composition comprising an aqueous extract of immortelle, an oily immortelle extract, an essential oil of immortelle and an essential oil of myrtle. The present invention also relates to a cosmetic cosmetic care process comprising the use of a cosmetic composition comprising an aqueous extract of immortelle, an oily immortelle extract, an immortelle essential oil and an essential oil of myrtle. The present invention finds application particularly in the cosmetic and dermatological fields.

Description

TECHNICAL FIELD The present invention relates to a cosmetic or dermatological composition comprising an aqueous extract of immortelle, an oily immortelle extract, an immortelle essential oil and an essential oil of myrtle. The present invention also relates to a cosmetic treatment method comprising the use of a cosmetic composition comprising an aqueous extract of immortelle, an oily immortelle extract, an immortelle essential oil and an essential oil of myrtle. The present invention finds application particularly in the cosmetic and dermatological fields. In the description below, references in brackets ([]) refer to the list of references at the end of the text.

State of the art The skin is the heaviest organ of the human body and has a very complex architecture. Barrier between the outer and inner environments of our body, its operation has two ends: to ensure communication between the body and the external environment and protect it from aggression. The functions of the skin are multiple, but four main roles are recognized: protection because the skin constitutes a physical, immunological and biological barrier, thermoregulation through its role in the maintenance of body temperature, metabolism through the hypodermis, place of main storage of triglycerides produced during lipogenesis and which can be mobilized for lipolysis and sensation at the origin of one of the five senses: the touch. Skin aging can lead to an alteration of body image perception with, ultimately, dysmorphophobic or depressive symptoms as described in Koblenzer CS. Psychological aspects of aging and the skin. Clinics Dermatol 1996; 14: 171-7 [1]. On the other hand, people with signs of skin aging are often perceived by our society as being in poor health and incapable of initiative (Koblenzer CS, Psychologic aspects of aging and the skin.

Clinics Dermatol 1996; 14: 171-7 [1]). Thus, the therapeutic demand to prevent skin aging is today stronger and stronger. To answer it, a better knowledge of its physiopathology, but also of its clinical consequences, is necessary. Like all other organs, the skin is subject to aging. Chronological, genetic, and endocrine factors (Pierard GE, Theory of climacteric skin aging, Dermatology 1996, 193: 273-4 [3]), such as postmenopausal changes in women, determine intrinsic skin aging. The skin is also subjected to extrinsic aggressions which, over time, amplify the phenomenon of intrinsic aging. The role of ultraviolet (UV) radiation is major. They are responsible for photo-aging. UVA rays are certainly at least as harmful as UVB rays as indicated in Petersen MJ, Hansen C, Craig S. Ultraviolet A irradiation stimulates collagenase production in cultured human fibroblasts. J Invest Dermatol 1992; 99: 440-4 [4]. Environmental factors, primarily tobacco and alcohol, also contribute to skin aging. Other radiations (infrared, X-rays, gamma rays, but probably also visible light), gravity occur to a lesser extent. 3003 16 7 3 A / Intrinsic aging: Biological mechanisms are linked to an imbalance between cellular degradation phenomena (active forms of oxygen, end-products of glycation, etc.) and repair systems (anti-oxidants, enzymes DNA repair, proteosomal enzymes, proteases, proteinases, phospholipases and acyltransferases, enzymes involved in link stability etc.). DNA repair mechanisms play a major role in the fight against aging. Several enzymatic systems cooperate to repair mutations spontaneously generated by the body or induced by external aggressions (eg UV radiation). B / Extrinsic aging: UV radiation is the main factor responsible for extrinsic aging. Its mechanism of action is twofold: either by direct interaction on cellular DNA (interference primarily due to UVB) or indirectly through the active forms of oxygen, which is the main mode of action of UVA ( West MD, The Cellular and Molecular Biology of Skin Aging, Arch Dermatol 1994; 130: 87-95 [5]). By interacting directly with the DNA, UVs induce the formation of photoproducts (especially thymidine dimers for UVB) that can lead to mutations, initiation of carcinogenesis, or even cell death. When the UV doses received are too great, the activation of the p53 protein makes it possible to cause the death of the keratinocyte by apoptosis phenomenon. UV radiation also increases the expression of metalloproteinases (MPs) in the epidermis and dermis (these enzymes degrade collagen and other macromolecules in the extracellular dermal matrix). Sun exposure of only 5 to 15 minutes at noon results in the production of collagenase, stromelysin-1 and gelatinase at 92 kDa. Collagenase hydrolyzes the collagen fibers, then stromelysin-1 and gelatinase of 92kd eventually degrade them. CI Histological, clinical and functional consequences Intrinsic aging and extrinsic aging thus add up to deleterious effects on all cutaneous structures. The respective part of the changes caused by intrinsic or extrinsic aging is often difficult to individualize because of the existence of sometimes conflicting bibliographic data. With age, the different skin structures are morphologically and functionally altered. The main consequences are a decline in defense, healing, perception and thermoregulation functions as well as the establishment of a chronic inflammatory state corresponding to an increase in the number of macrophages and persistent dry skin (Harvell JD, Maibach HI, Percutaneous uptake and inflammation in aged skin: a review, J Am Acad Dermatol 1994; 31: 1015-21 [6]). On the morphohistological level, intrinsic skin aging is manifested by atrophy of the dermis, that is to say a reduction in the number of fibroblasts and mast cells, and the epidermis. In the epidermis, the turnover of the epidermis goes from 21 days to 40 days hence the negative impact on healing and tissue repair (Grove GL, Kligman AM Age-associated changes in human epidermal cell renewal J Gerontol 38: 137-42 [7]). The keratinocytes are decreasing in size and their number is decreasing. Cell renewal slows down and cohesion between the dermis and the epidermis diminishes. In the dermis, the collagen fibers break, their natural renewal becomes more difficult, elastin loses its elasticity. The dermis gradually loses its density, it thins, wrinkles and fine lines appear.

Extrinsic aging leads to a reduction in both the number of collagen fibers and their quality. It causes the accumulation of poor quality elastic fibers, thus reducing the elasticity of the skin. Like intrinsic aging, it slows the proliferation of cells. Finally, it reduces the uniformity of pigmentation, which gives the skin a duller and older appearance.

Many anti-aging compositions are known in the state of the art. However, these compositions do not allow both to fight, in a systematic and pronounced manner, against the effects of skin aging while protecting the skin from external aggression.

There is therefore a real need to find a new compound, composition and / or a means for simultaneously treating skin aging and / or causes causing aging while protecting the skin from external aggression palliating all of defects, disadvantages and obstacles of the prior art cited above.

SUMMARY OF THE INVENTION The present invention is specifically intended to meet this need by providing a cosmetic or dermatological composition comprising an aqueous extract of immortelle, an oily immortelle extract, an essential oil of immortelle and an essential oil of myrtle. The present invention also relates to the cosmetic and / or dermatological use of this composition. In particular, the present invention relates to the cosmetic and / or dermatological use of a cosmetic or dermatological composition comprising an aqueous extract of immortelle, an oily immortelle extract, an immortelle essential oil and an essential oil of myrtle. As used herein, "extract" means an extract obtained for example from a plant and / or part of a plant. It may be for example an extract obtained from flowers or flower heads, leaves, seeds, roots, stems, seeds, pericarp. It may be for example an extract obtained from whole flowers.

In the present "immortelle" is meant a species of flowering plants of the family Asteraceae and genus Helichrysum. In particular, it may be Helictysum itaticum which may be present all around the Mediterranean. It may be for example the immortelle described in French Patent Application No. 01/11224. In the present "aqueous extract" is meant any aqueous extract obtained by any method known to those skilled in the art. For example, it can be obtained by aqueous extraction, by hydrodistillation, hydro-glycerinated or hydro-alcoholic extraction.

According to the invention, the aqueous extract of immortelle can be any aqueous extract of immortelle known to those skilled in the art. It may be, for example, a biological aqueous extract of Immortelle, for example a biological immortelle extract from Corsica from Greentech. According to the invention, the aqueous extract may comprise, for example, from 0.1 to 0.3% of sugars, for example 0.2% by weight of sugars, from 1 to 2 ppm of vitamin E, for example 1.7 ppm. of vitamin E and from 0.2 to 0.5% by weight of polyphenols, for example 0.44% by weight of polyphenols including 5Oppm of caffeic acid. As used herein, "oily extract" means an extract rich in fatty acid and / or fat and / or a non-water soluble extract. According to the invention, the oily extract may be any oily extract obtained by any method known to those skilled in the art. For example, it can be obtained by oily extraction by microwaves, microwave, supercritical extraction, maceration in an oily solvent, ultrasonic assisted oily extraction. It may be, for example, an organic oily extract of immortelle by microwave extraction assisted by ultra sounds according to a process comprising a first step of grinding the immortelle flowering top followed by an ultrasound-assisted extraction and by microwaves, then a clarification and finally a filtration step to recover the oily extract.

As used herein, the oily immortelle extract can be obtained from a plant and / or part of a plant. It may be for example an oily extract obtained from flowers or flower heads, leaves, seeds, roots, stems, pericarp.

As used herein, the oily immortelle extract can comprise from 0.1 to 0.3% by weight of sugars, for example 0.2% of sugars, from 0.04 to 0.06% by weight of vitamin E , for example 0.051% of vitamin E, from 99% to 99.9% by weight of fatty acids, for example 99.8% by weight of fatty acids, of which from 80 to 85% of oleic acid, for example 83.4% oleic acid, 6 to 8% linoleic acid (omega-6), 2 to 5% palmitic acid and 2 to 4% stearic acid, but is particularly distinguished by its content in phytosterols from 0.2% to 0.5%, for example 0.35% of which a majority of beta-sitosterol 0.2% and polyphenols of 0.01 to 0.1%. In the present invention, the sterol profile can be measured according to any known method, for example according to ISO 1228; the dosage of vitamin E can be measured according to any known method, for example according to standard NF V18-402; the fatty acid profile may be measured according to any known method, for example according to standard NF EN ISO 5508/5509; the profile of the sugars can be measured by any known method, for example by a chromatographic technique such as ion chromatography, the total polyphenol assay can be measured by any known method, for example according to the ISO 14502-1 standard. In the present "essential oil" means any essential oil obtained by any method known to those skilled in the art. For example, it can be obtained by hydrodistillation. It may be for example a biological essential oil of immortelle, that is to say from organic farming, for example a biological essential oil of Corsica, companies TEPPE ROSSE, STEPHAN FRANCISCI, GAEC of ASTRATELLA or a mixture of two or three of these oils. As used herein, the essential oil can be obtained, for example from a plant and / or part of a plant. It may be for example an oil obtained from flowers or flower heads, leaves, seeds, roots, stems. As used herein, the organic essential oil of immortelle can comprise from 35 to 50% by weight of neryl acetate.

Whatever the nature of the immortelle extract, the extracts can be obtained by any of the techniques known to those skilled in the art, for example supercritical or hydroalcoholic extraction. The term "cosmetic composition" is intended to mean any cosmetic composition, for example a composition that can be brought into contact with the superficial parts of the human body, for example the epidermis, the hair and capillary systems, the external organs, the teeth and mucous. According to the invention, the aqueous extract of immortelle may be present in the composition, for example at a concentration of 0.01 to 10% by weight relative to the total weight of said composition, for example from 0.01 to 5% by weight relative to the total weight of said composition. According to the invention, the oily immortelle extract may be present in the composition, for example at a concentration of 0.001 to 5% by weight relative to the total weight of said composition, for example from 0.001 to 2% by weight relative to to the total weight of said composition. According to the invention, the immortelle essential oil may be present in the composition at a concentration of from 0.001% to 2% by weight relative to the total weight of said composition, for example from 0.001% to 1% by weight relative to total weight of said composition. According to the invention, the sum of the aqueous, oily extract and the essential oil in the composition of the invention may be from 0.001 to 16% by weight relative to the total weight of said composition, for example from 0, From 1 to 8% by weight relative to the total weight of said composition.

According to the invention, the essential oil of myrtle may be, for example, an essential oil of green myrtle, for example an essential oil of myrtle green Corsican. This oil can for example be a biological oil. According to the invention, the essential oil of myrtle may be present in the composition at a concentration of, for example, from 0.001% to 1%, for example from 0.01% to 0.5%. The composition according to the present invention may further comprise spherulites of myrtle essential oil, for example of Myrtus communis essential oil, for example an essential oil of green myrtle, for example of corse green myrtle essential oil, for example, organic essential oil of Corsican green myrtle. It may be for example the essential oil marketed by the company GAEC ASTRATELLA. Herein, the concentration of spherulites of myrtle essential oil can range, for example, from 0.05% to 7%, for example from 0.1% to 3%. The composition according to the present invention may furthermore comprise spherulites of immortelle essential oil, for example essential oil of Helichtysum italicum, for example an essential oil of immortelle Corse, for example a biological essential oil. immortal Corsica. Herein, the spherulite concentration of immortelle essential oil may be, for example from 0.05% to 7%, for example from 0.1% to 3%. Spherulites are vesicles composed of alternating lipid bilayers of about one micron. Advantageously according to the invention, the composition comprising essential oil of myrtle, an aqueous extract of immortelle, an oily immortelle extract, an essential oil of immortelle advantageously makes it possible to simultaneously increase the smoothing, the tonicity of the 30 skin via synergistic stimulation of the expression of genes coding for the proteins constituting corneocytes and genes involved in the metabolism of sphingolipids. In particular, the composition according to the invention advantageously and simultaneously makes it possible to reduce wrinkles and fine lines while plumping the skin. According to the invention, the composition may further comprise honey. It may be for example a spring honey from Corsica, for example Corsican honey marketed by beekeeper producer Franck Dupré located in Calenzana. According to the invention, the honey may be present in the composition, for example at a concentration of from 0.01 to 50% by weight relative to the total weight of the composition, for example from 0.1 to 15%, for example from 0.1 to 5%, for example from 0.1 to 2% by weight relative to the total weight of the composition. Advantageously according to the invention, the composition comprising an essential oil of immortelle, an aqueous extract of immortelle, an oily extract of immortelle, an essential oil of myrtle and honey advantageously makes it possible to treat simultaneously and in a synergistic manner the signs of aging skin, for example wrinkles / fine lines, age spots, increase the suppleness and tone of the skin via the stimulation of genes involved in the cohesion of cutaneous cells, in the structure of the collagen fibers, and in cell repair. In particular, the composition according to the invention advantageously makes it possible to reinforce the skin barrier effect and / or to protect the skin from external aggressions such as ultraviolet rays via, in particular, the synergistic stimulation of the expression of genes encoding Proteins Involved in the Reinforcement of the Extracellular Matrix and Genes Involved in DNA Repair According to the invention, the composition may further comprise the hydroglycerine extract of water clover. In the present "water clover" is the Menyanth 30 or water clover, Menyanthes trifoliata.

According to the invention, the hydroglycerinated extract of water clover can be, for example, the extract sold by the company Codif under the catalog reference hydrofiltrate Menyanthes G. According to the invention, the hydroglycerine extract of water clover can be present in the composition for example at a concentration of 0.01% to 15% by weight relative to the total weight of said composition, for example from 0.01 to 5% by weight relative to the total weight of said composition. The hydroglycerine extract of water clover makes it particularly advantageous to firm the skin and improve the oval of the face. It also has antioxidant properties, particularly advantageously to prevent skin aging. According to the invention, the composition may comprise, for example a compound selected from the group comprising a hyaluronic acid derivative, for example calcium hyaluronate, sodium hyaluronate, an extract of Bellis Perennis, a mixture of vegetable oils evening primrose, borage, echium and camelina. According to the invention, the Bellis Perennis extract may be present in the composition, for example at a concentration of 0.01 to 15% by weight relative to the total weight of the composition, for example from 0.01 to 5% by weight per relative to the total weight of said composition. According to the invention, when the Bellis Perennis extract is an aqueous extract, it may be for example the extract sold by the company CLR under the reference Belides catalog. The aqueous extract of Bellis Perennis advantageously allows in particular to reduce the appearance of pigmentation spots. According to the invention the hyaluronic acid derivative may be present in the composition, for example at a concentration of 0.001 to 3% by weight relative to the total weight of the composition. According to the invention, the hyaluronic acid derivative may be for example the "Hyaluronic acid sodium knows" marketed by Laserson.

The hyaluronic acid derivative advantageously makes it possible in particular to improve the hydration of the skin. According to the invention, the composition may furthermore comprise at least one oil chosen from evening primrose vegetable oil, borage oil, echium oil and / or camelina oil. According to the invention, the evening primrose vegetable oil may be any evening primrose vegetable oil known to a person skilled in the art, it may be, for example, the organic evening primrose oil marketed by the company DSKH. According to the invention borage oil may be any borage plant known to those skilled in the art, it may be for example organic borage oil marketed by the Emile Noël oil mill. According to the invention, the echium oil may be any echium oil known to those skilled in the art, for example it may be HU echium Plantagineum oil marketed by Croda.

According to the invention the camelina oil can be any camelina oil known to those skilled in the art, it may be for example organic camelina oil marketed by the company Codina, the oil "gold of pleasure Marketed by IMCD. According to the invention, said at least one oil chosen from vegetable oil of evening primrose, borage, echium and camelina may be present in the composition for example at a concentration of 0.1 to 10% by weight relative to to the total weight of said composition, for example from 0.1 to 5% by weight relative to the total of said composition According to the invention, the composition may furthermore comprise a mixture of vegetable oil of evening primrose, borage, d echium and camelina. According to the invention, the vegetable oil mixture of evening primrose, borage, echium and camelina may be present in the composition, for example at a concentration of 0.1 to 10% by weight relative to the total weight of said composition, for example from 0.1 to 5% by weight relative to the total of said composition.

According to the invention, the cosmetic composition may be in any form known to those skilled in the field of cosmetics and dermatology, without any particular pharmaceutical restriction.

The cosmetic or dermatological composition may be in any galenical form known to those skilled in the art, for example in the form of an ointment, a cream, an oil, a milk, an ointment, a powder, a stick, a soaked swab, a transdermal patch, a solution, a gel, a serum, a spray, a lotion or a suspension . It may be for example a cream, a milk, a gel, a lotion, an ointment, a transdermal patch. The cosmetic or dermatological composition according to the present invention may be, for example a composition for the care of the face, of the body, for example compositions for the face and / or the body.

The cosmetic or dermatological composition of the invention can be obtained by any appropriate method known to those skilled in the art for the manufacture of a cosmetic and / or dermatological composition. It may be, for example a simple mixture. It may also be, for example, a process comprising a step of incorporating an internal phase into an external phase by means of an emulsifier, for example a rotor-stator turbine. It may also be for example a method using the Phase Inversion Temperature (TIP) method conventionally used by those skilled in the art to obtain oil-in-water emulsions whose dispersed droplets are particularly fine, for example with a diameter of 0.1 to 1 μm. According to the invention, the cosmetic composition may be a composition used in an anti-aging treatment. It may be, for example, a cosmetic composition for the treatment of cutaneous aging, for example wrinkles and fine lines. The present invention also relates to a cosmetic treatment method comprising the application to the skin of a cosmetic composition comprising an aqueous extract of immortelle, an oily extract of immortelle, an essential oil of immortelle and an essential oil of myrtle. The aqueous extract of immortelle, an oily extract of immortelle, an essential oil of immortelle and an essential oil of myrtle are as defined above. The cosmetic composition may also further comprise honey and / or a clover extract of water and / or an extract of Bellis perennis and / or a derivative of hyaluronic acid and / or a mixture of vegetable oils. The honey, the water clover extract, the Bellis perennis extract, the hyaluronic acid derivative and the vegetable oil mixture are as defined previously. The present invention also relates to a cosmetic treatment method comprising the application on the skin of a composition according to the invention. According to the invention, the cosmetic treatment may be an anti-aging treatment, a protective treatment, a repairing treatment, a smoothing treatment. For this application, it is possible, for example, to use the dosage forms described above. The application to the skin can therefore be carried out according to the dosage form used. This may be, for example, a simple application to the skin or an application accompanied, for example, by a skin massage with the composition of the present invention. The application is preferably carried out with a sufficient amount of the composition so that the entire surface of the skin to be treated is treated. It may be for example a conventional application, such as a cream on the skin. For example, it may also be an application for forming a treating mask.

The application can be any application desired by the user, for example a daily, twice daily, weekly and / or bi-monthly application. This may be for example an application once a day, twice a day or more. The present invention also relates to a cosmetic care kit comprising a cosmetic composition according to the present invention and a manual or instructions for use. The kit can be for example a packaging. The manual or this notice may have the particular function of explaining to the user the manner and frequency of application to the skin of the composition of the present invention. According to the invention, the cosmetic care kit may also comprise an applicator, for example a spatula, a brush, a dynamic applicator, for example a massage device impregnated with the cosmetic composition of the invention. EXAMPLES Example 1: Example of a cosmetic composition for the care of the face (cream) A base composition described in Table 1 below was prepared by mixing the various components indicated in Table 1 below: Table 1: Composition base ingredients (cream) Ingredients Quantity in% by weight based on the total weight of the composition (%) Alkyl polyglucoside 2-5 Hard fat compounds 1-2,5 Capric capric triglycerides 5-15 Ester 10-20 Vegetable oils 1 -5 Glycerin 3-8 Acrylate Gelling Agent 1-2 Trace Elements 0.1-1 Vitamin C Derivatives 0.5-2 Scent Qs Preservatives, Antioxidants, pH Regulator Qs Water qs 100% The base composition was prepared according to the following process: the glycerine was introduced into water and mixed at 1500 rpm, then the mixture was heated to 80 ° C the gelling agent was dispersed in this mixture with stirring at 1500 rpm until dissolution . the oily phase previously prepared by mixing, for example emulsifier, hard fat, caprylic capric triglycerides, ester and vegetable oils, at 80 ° C., until a homogeneous mixture, was introduced with stirring at a speed of 1500 rpm - the cream thus obtained was then cooled to 40 ° C with stirring at 1500 rpm - the active ingredients, for example, trace elements, the derivative Vitamin C, as well as the fragrance, preservatives and pH regulator were then added. From this base composition, for facial care, 6 different compositions were made by adding the different active ingredients in the proportions indicated in Table 2 below: Table 2: percentage by weight of the compounds in the composition Composition% in weight% by weight% by weight ° A) by weight% by weight of honey relative to the total weight ° A) by weight% by weight% by weight of hyaluronic acid derivative relative to the total weight of the composition of extract of oil extract of clover extract of Bellis Perennis in relation to the total weight of the composition (%) of aqueous oily essential essential water composition by (%) immortelle of everlasting immortelle myrtle ratio (%) relative to the total weight to weight to weight total weight of the total of the total of the total of the composition composition composition composition composition (%) (%) (%) (%) (%) No. 1 0.1 0.03 0 , 075 0.045 0.1 2 1 0.002 No. 2 0.01 0.01 0.02 0.01 0.01 0.1 0.1 0.001 No. 3 0.1 0.03 0.075 0.045 0.1 2 1 - No. 4 0.1 0.03 0.075 0.045 0.1 2 - 0.002 No. 5 0.1 0.03 0.075 0.045 0.1 - 1 0.002 No. 6 0.2 0.03 0.075 0.045 0.2 - 1 - 00 Example 2: Example of a cosmetic composition for the care of eyes (Divine Gaze) A basic composition described in Table 3 below was prepared by mixing different components: Table 3: base composition for the face ( Regard) Ingredients Quantity in% by weight relative to the total weight of the composition (%) Alkyl polyglucoside 2-5 Shea butter 0.5-2 Caprylic capric triglycerides 1-5 Ester 10-20 Vegetable oils 0.5-5 Glycerin 3 -8 Carbomer 0.1-0.5 Wheatwort extract 0.1-5 Ash extract 0.1-5 Rose water 1-10 Preservatives, antioxidants, pH regulator Qs Water qs 100% The composition of The base was prepared according to the following process: - the glycerin was introduced into the water and mixed with 15 00 rpm, then the mixture was heated to 80 ° C - the gelling agent was dispersed in this mixture with stirring at 1500 rpm until dissolution. the oily phase previously prepared by mixing, for example emulsifier, shea butter, caprylic capric triglycerides, and vegetable oils, at 80 ° C., until a homogeneous mixture is obtained; The cream thus obtained was then stirred at a speed of 1500 rpm. The cream thus obtained was then cooled to 40 ° C. with stirring at 1500 rpm. The active ingredients, for example, black wheat wax extract, ash extract, rose water as well as preservatives and pH regulator were then added. From this base composition, for eye care (Divine Gaze), 6 compositions were made by adding different actives in different amounts: Table 4:% by weight of the compounds in the composition Composition% by weight% by weight% by weight% by weight% by weight of honey relative to the total weight of extract of oil extract of the oil oily essential essential composition of immortelle immortelle of immortelle of myrtle by (%) relative to ratio to weight to weight to weight total total weight of total of total composition composition composition composition (%) (%) (%) (%) No. 1 0.01 0.01 0 , 01 0.005 0.01 No. 2 0.001 0.001 0.002 0.001 0.001 No. 3 0.1 0.03 0.01 0.005 0.1 No. 4 0.2 0.01 0.01 0.005 0.1 No. 5 0 , 02 0.02 0.01 0.005 0.02 No. 6 0.1 0.01 0.01 0.005 0.1 Example 3: Example of a cosmetic composition for the care of the face (Divine Extract) A basic composition described in table The following is prepared by mixing different components: Table 5: Base composition for the face (Extract) Ingredients Quantity in% by weight relative to the total weight of the composition (`) / 0) Alkyl polyglucoside 2-5 Ester 0.5-5 Vegetable Oils 0.5-5 Glycerin 3-8 Gelling Agents (Xanthan Gum, Acrylate) 0.1-0.5 Vitamin C Derivative 0.5-2 Trace Elements 0.1-1 Preservatives, Antioxidants pH regulator Qs Water qs 100% The base composition was prepared according to the following method: the glycerine was introduced into water and mixed at 1500 rpm, and then the mixture was heated to 80 ° C. the gelling agents were dispersed in this mixture with stirring at 1500 rpm until dissolution. the oily phase previously prepared by mixing, for example with emulsifier, ester and vegetable oils, at 80 ° C., until a homogeneous mixture is obtained, was introduced with stirring at a temperature of 1500 rpm speed - the cream thus obtained was then cooled to 40 ° C with stirring at 1500 rpm - the active ingredients, for example, the derivative of vitamin C, the trace elements as well as the perfume, the preservatives the antioxidants and the pH regulator were then added. From this base composition, for facial care (Divine extract), 6 compositions were made by adding different active ingredients in different amounts: Table 6: percentage by weight of the compounds in the composition Composition% by weight% by weight% by weight% by weight% by weight of honey relative to the total weight% by weight% by weight c1 / 0 by weight of hyaluronic acid derivative relative to the total weight of the extract oil extract oil composition water clover compared to the total weight of (%) essential oily aqueous extract composition of the immortelle immortelle immortelle Bellis Perennis composition of myrtle immortelle by (%) composition compared to relative to ratio in relation to (%) total weight of product by weight to weight by weight total weight total composition of total of (%) composition composition composition composition (%) (%) (%) (%) No. 1 0.1 0.03 0.08 0.05 0.2 2 1 0.022 No. 2 0.01 0.01 0.0 2 0.01 0.01 0.1 0.1 0.01 No. 3 0.1 0.03 0.08 0.05 0.2 2 1 - No. 4 0.1 0.03 0.08 0 , 05 0.2 2 - 0.022 No. 5 0.1 0.03 0.08 0.05 0.2 - 1 0.022 No. 6 0.2 0.03 0.08 0.05 0.2 - 1 - Example 4: Example of a Cosmetic Composition for Facial Care (Divine Lotion) A base composition described in Table 7 below was prepared by mixing different components: Table 7: Basic Composition for the Face (Lotion) Ingredients Quantity in% by weight with respect to the total weight of the composition (io) Glycerine 3-8 Gelling agents (xanthan gum, acrylate, cellulose) 0.1-0.5 Immortelle floral water 0.5-5 Trace elements 0.1- 1 Preservatives, pH regulator Qs Water qs 100% The base composition was prepared according to the following process: the glycerin was introduced into water and mixed at 1500 rpm, then the mixture was heated to 60 ° The gelling agents were dispersed in this mixture with stirring at 1500 rpm until dissolution. The active ingredients were cooled to 40 ° C. with stirring at 1500 rpm, for example trace elements, immortal floral water, perfume, preservatives and pH regulator were then added.

From this base composition, for facial care (Divine Lotion), 6 compositions were made by adding different active ingredients in different amounts: Table 8: Weight percentage of the compounds in the composition Composition% by weight% by weight% in weight% by weight% by weight% by weight ° / 0 by weight of hyaluronic acid derivative relative to the total weight of the aqueous immortelle extract composition relative to the total weight of the extract composition honey oil oil per clover of water in relation to the total weight (%) (%) essential essential oil compared to the immortelle of immortelle of myrtle by total weight composition compared to the ratio of the (%) ) weight to weight total weight total composition of the total of the (%) composition composition composition (%) (%) (%) No. 1 0.1 0.03 0.03 0.01 0.1 2 0.002 Nr 2 0.01 0.01 0.02 0.01 0.01 0.1 0.01 Nr 3 0.1 0.03 0.03 0.01 0.1 2 - No 4 0, 1 0.03 0.03 0.01 _ 2 0.002 No. 0.103 0.03 0.01 0.1-0.002 No.6 0.1 0.03 0.03 0.01 0.1 - -N) Example 5: Anti-aging effect of composition of the invention The purpose of these experiments was to validate ex vivo by a transcriptomic analysis by DNA chips, the stimulation of the constitution proteins which have an action on the integrity of the corneocytes and the stimulation of the metabolism of shingolipids by an example composition according to the invention. The transcriptome is the set of messenger RNAs, molecules that serve as a template for the synthesis of proteins, resulting from the expression of a part of the genome of a cellular tissue or a cell type. The characterization and quantification of the transcriptome in a given tissue and under given conditions made it possible to identify the active genes, to determine the mechanisms of regulation of gene expression and to define the expression networks of the genes. One of the techniques used to simultaneously measure the level of expression of a large number of different types of messenger RNA is that of the DNA chip as described in the publication of Seltz et al (Seitz G, Bonin M, Fuchs J et al Inhibition of glutathione-S-transferase as a treatment strategy for multidrug resistance in childhood rhabdomyosarcoma International Journal of Oncology 2010 36: 491-500 [2]). DNA microarrays can measure and visualize very quickly the differences in expression between genes at the level of a complete genome. 1. Material and method 1.1 Preparation of samples: After carrying out a cell viability test, according to the method described in T. Mosmann's publication (Tim Mosmann, Rapid Colorimetric Assay for Cell Growth and Survival: Application to Proliferation and Cytotoxicity Assays. Pages 55-63 [81] to determine for each extract the maximum non-toxic diluted concentration to be applied in-vitro, skin explants originating from facial facelift. One patient was incubated independently in the presence of each extract or mixtures of 3 or 4 of them for 24 hours in DMEM (DulbeccoNogt modified Eagle's minimal essential medium) without serum at 37 ° C in a humid atmosphere at 5% CO 2. . An untreated skin explant was incubated under the same conditions. Table 9 summarizes the concentrations applied for each extract. Table 9: non-toxic maximum concentrations expressed in% for the myrtle essential oil, the aqueous extract of immortelle, the oily immortelle extract, the essential oil of immortelle and the honey composition% by weight essential oil of 1 X 10 -5 Myrtle Aqueous Extract 0.5 Immortelle Oily Extract 0.1 Immortelle Essential Oil 1 X 10-5 Immortelle Honey 1 1.2 Extraction of Total RNAs and Linear Amplification After incubation, skin explants were crushed and then the total RNAs from each explant were extracted using the RNeasy Kit (Qiagen, Hilden, Germany). The total RNAs were then quantified and then the quality was verified using the BlOanalyzer 2100 with the RNA 6000 Nano LabChip Kit (Agilent Technologies, Boblingen, Germany). An INR obtained between 7.5 and 10 attests to the good quality of the total RNAs from each explant previously incubated or not with myrtle essential oil, an aqueous extract of immortelle, an oily immortelle extract, an oil immortelle essential oil or mixture thereof, or with a mixture comprising myrtle essential oil, an aqueous extract of immortelle, an oily immortelle extract, an immortelle essential oil or with a mixture comprising oil essential oil of myrtle, an aqueous extract of immortelle, an oily extract of immortelle, an essential oil of immortelle and honey. 1.3 Measurement of Gene Expression on an Oligonucleotide Chip and Data Acquisition: The Affymetrix Human Gene 1.0 ST Whole Genome oligonucleotide chips were used for each gene expression analysis (GeneChip HU Gene 1.0, Affymetrix, Santa Clara, USA). Full documentation and the experimental protocol [8] is available at: http://media.affymetrix.com/support/technical/datasheets/gene_1_0_st_dat asheet.pdf. . More simply, the cycles of retrotranscription and amplification of the total RNAs in cRNA then in cDNA were carried out using the Ambion WT Expression kits (Ambion, Applied Biosystem, USA) and the GeneChip WT kit Terminal Labeling and Hybridization User (Affymetrix). The hybridization of the chips and the measurements were carried out by Microarray Facility Tubingen. The chips were then scanned using the Gene Chip GCS3000 scanner (Affymetrix) and the GCOS 1.4 software. The scanned images were then analyzed using the Expression 1.0 (Affymetrix) console to convert light intensity measurements into digital data and normalize this data. Comprehensive data analysis and statistical analysis were performed using Genespring GX 11 software (Agilent Technologies) based, in particular, on classification algorithms. Differences in gene expression thus obtained following the application of the different extracts and / or their mixture on skin explants with respect to an untreated explant are collated in Tables 10, 11 and 12. differentially expressed genes between untreated skin and treated skin has a p-value <0.01 (very significant statistical value).

Table 10: Difference in gene expression and biological significance of the genes following incubation in the presence of myrtle essential oil GENES db EST FUNCTIONS STATUS: stimulation compared to untreated skin CXCL1 (BT006880.1 Regulates cellular traffic and x 10 "chemoki signaling of different types of ne (CXC leukocytes via G. motif receptors) ligand 1") CXCL2 NM 0020e ° Regulates cellular trafficking and the x 8 ("chemoki ne (CXC` '' 'signaling different types of motif) 3 leukocytes via G. ligand receptors 2 ") CXCL5 NM 002994 Is a chemokine involved in x 2.5 (" chemoki - 'activation of neutrophils only (CXC 3 motif) ligand 5 ") CYP27B1 NM 000785. Mitochondrial monoxygenase x8 ("polypeptide 3 involved in the elimination of 1 of drugs and toxins / plays a role in cytochrom synthesis of active vitamin D P450, family 27, subfamily B") STEAP4 BCO20600.1 Participate in the regulation of e x 3 (member 4 insulin sensitivity of human adipocytes / is decreased in family obese patients and may play a role STEAP) important in the development of human obesity. P4HA2 Key enzyme in the synthesis of x4 ("alpha collagen, polypeptide II prolyl-4-hydroxylase") P4HA1 BC034998.1 Key enzyme in the synthesis of x6 ("alpha collagen, polypeptide and prolyl-4-hydroxylase") ") UGDH AJ007702.1 Participates in the biosynthesis of x 2 UDP-glycosaminoglycans such as (" hyaluronan glucose, 6-chondretin sulfate, and heparan sulfate, dehydrogenated plays a role in nase transduction ") signal, the cell migration. ITGB1 BCO20057.1 Members of the x2 family (integrin integrins are beta 1 receptors) membrane involved in cell adhesion and resulting in various biological processes (repair / immunity / embryogenesis) ITGB3 NM 000212. Members of the family of x 4 (integrin 2 integrins are beta 3) membrane receptors involved in cell adhesion and resulting in various biological processes (repair / immunity / embryogenesis) ITGA5 NM_002205. Forms a fibronectin x 4 receptor ("integrin 2 and is involved in alpha 5 cell surface signaling") SOD2 BT006967.1 This protein binds x6 superoxides ("superoxins derived from oxidative phosphorilation and converts them into oxygen This protein binds ubiquinone, which is a protein that allows the transfer of electrons. It binds an important role in induced angiogenesis-induced hypoxia. ubiquinol- the elimination of cytochrom reactive species of oxygen ec reductase "ubiquinol - cytochrom ec reductase binding protein") TNF (tumor necrosis factor) NM 000594. - involved in the regulation of an x4 2 wide range of biological processes, including cell proliferation, differentiation, apoptosis, lipid metabolism TNFAIP6 NM 007115. involved in the stability of x 35 (protein 3 matrix extracellular and cell-induced alpha migration 6 necrotizing tumor factor "tumor necrosis factor, alpha-induced protein 6") HSPA1B NM_005346. stabilizes existing proteins and x 6 (protein 4 protects functional shock proteins against heat shock protein 1) HSPA6 NM_002155. stabilizes existing proteins and x 10 (protein 3 protects the functional proteins from shock against aggregation / thermal effects cytoprotective 6 "heat shock protein 6") IL1RL1 BC030975.1 a member of the x 8 family (receptor receptors of the interleukin 1, type-involved in the function of helper interleukin T-cells e 1 "receptor-like 1" HSPA1A / h NM_005345 stabilizes existing proteins and x9 (protein 5 protects functional proteins from shock against aggregation / cytoprotective thermal effects 1A "Heat Shock Protein 1A") HIST2H2A NM_003517. Histones are nuclear basic 2-core histone 2 x4 C proteins that are responsible for the structure of H2ac nucleosomes, physically protecting DNA and preserving the integrity of the ubiquitin genome AF201370. 1 It interacts with cullin and plays a ligase x 5 role in the progression of RNF168 cell cycle HIST2H2B NM 003528. Histones are proteins x4 E 2 2 nuclear basic which are (histone responsible for cluster 2 structure, nucleosomes, protecting H2be) physically DNA and preserving the integrity of the genome GADD45A M60974.1 Gene responding to environmental stress X4 DCD NM 053283. expressed constitutively in x 3 (dermcidin 2 sweat and has e) antibacterial and antifungal activities OXSR1 NM_005109. Regulates downstream kinases in x3 (protein1 2 response to environmental stress response oxidative stress "oxidative -stress responsive 1") TXNRD1 AY423378.1 Role in the metabolism of x 2.5 (thioredoxi selenium and protection against ne oxidative stress reductase 1) GSTM1 BC036805.1 This enzyme eliminates x2.5 (glutathione carcinogens, drugs S-therapeutics, environmental transferas toxins, compounds e mu 1) from oxidative stress in partnership with gluthation GSTM2 BT019947. 1 This enzyme eliminates x 2 (carcinogenic glutathione, e-therapeutic drugs, environmental transferase toxins, e mu 2 compounds) from oxidative stress in partnership with PIGA glutathione D11466.1 Protein required for x3 synthesis phosphatid N-acetylglucosaminyl ylinositol phosphatidylinositol (GIcNAc-PI) glycan anchor biosynthes is, class A VLDLR D16494.1 plays a very important role in x 3 (metaboli receptor triglycerides and in the signaling pathway of the very low density lipoprotein e lipoprotein e) FABP3 NM 004102. - Participates in cellular uptake, intracellular metabolism and / or fatty acid transport long chains. x2 (fatty acid binding protein 3) FABP5 NM 001444. Participates in cell uptake, intracellular metabolism and / or long-chain fatty acid transport. x 2 (fatty acid binding protein 2) Nedd4-2 AF210730.1 Plays in the sodium transport x4 E3 epithelium by regulating ubiquitin the cell surface expression of sodium channel ligase HLA-M21008.1 plays a central role in the immune system by presenting peptides derived from extracellular proteins immunizing x 4 DQB1 (major complex histocom-class II, DQ beta 1) In the above table, x N represents the factor As shown in Table 10 above, myrtle essential oil stimulates a large number of genes that may have effects, for example at the level of tight junctions and / or or at the level of detoxification of the cells. Table 11: Difference in Gene Expression Following Incubation in the Presence of a Mixture of Immortelle Essential Oil, Aqueous Immortelle Extract, Oily Immortelle Extract and Myrtle Essential Oil GENES dbEST FUNCTIONS STATUS: stimulation compared to untreated skin Late cornified envelope 1C (LCE1C) NM 178351.3 Involved in the x 11 terminal differentiation of keratinocytes and constitute a family of proteins with others that constitute stratum corneum LCE2B NM _014357.4 Involved in the x terminal differentiation of keratinocytes and constitute a family of proteins with others that constitute stratum corneum LCE2C NM_178429.2 Involved in X 80 terminal differentiation of keratinocytes and constitute a family of proteins with others that constitute stratum corneum SPRR2C BC120937.1 Involved in the x22 (small protein 2C late differentiation rich in proline keratinocytes and 'small proline-r ich constitutes a family of protein 2C ') structural proteins forming the horny envelope with involucrine and loricrin SPRR2E NM - Implicated in the x 20 (small protein 2E late differentiation rich in proline keratinocytes and' small proline-rich constitutes a family of protein 2E ") structural proteins forming the horny envelope with involucrine and loricrin SGPL1 NM 003901.3 Enzyme involved in x 2 with the (sphingosine-1-metabolism of phosphate lyase 1) sphingolipids, important aqueous lipid extract , oily constituents and cell oil membranes and intercorneocyte essential space of immortelle x 4 with aqueous extract mixture, oily, essential oil of immortelle and myrtle essential oil PSAPL1 NM 001085382.1 - No defined function X 4.5 (prosaposin -like 1) SMPD3 NM 018667.3 Enzyme involved in x 4.5 (sphingomyelin metabolism of phosphodiesterase sphingolipids, 3) important complex lipid constituents cell membranes and intercorneocyte space ARV1 BC001977.1 Enzyme involved in x 2 the metabolism of sphingolipids, important complex lipid constituents cell membranes and intercorneocyte space ELOV1 NM 001146393.1 Enzyme involved in x 2.5 (protein 1 the long chain metabolism of long chain fatty acid chains constituting fatty acid cell chain chains in the above table, x N represents the multiplication factor of the stimulation by compared to untreated skin. As demonstrated in Table 11 above, the mixture of immortelle essential oil, aqueous immortelle extract, immortelle oily extract and myrtle essential oil stimulates the expression of genes coding for : - proteins of constitution of the stratum corneum which have an action on the integrity of the corneocytes allowing, in addition, a smoothing of the skin on the surface via the obtaining of a more regular stratum corneum - the metabolism of the sphingolipids which are involved in the cellular cohesion and advantageously allows a strengthening of the skin barrier. Thus, the composition of the invention advantageously makes it possible to reinforce the surface smoothing of the skin, to reinforce the barrier effect, to treat the effects of aging, for example by reducing wrinkles and by increasing the firmness and elasticity, while protecting the skin from external aggressions.

Table 12: Difference in gene expression and biological significance of the genes following incubation in the presence of a mixture of essential oil of immortelle, aqueous extract of immortelle, oily extract of immortelle, of essential oil of myrtle and honey GENES dbEST FUNCTIONS STATUS: stimulation compared to untreated skin PRG4 proteoglycan U70136.1 This gene codes for a large x 3 4 or proteoglycan lubricine whose function is cartilage, hydrate the matrix extracellular and maintain dermal elasticity LUM Lumican NM_002345.3 This gene encodes a proteoglycan involved in the organization of collagen fibers and x 3 their spacing SPDYA Speedy BC093005.1 This gene codes for x 2 a protein involved in cellular protection following DNA damage EX01 exonuclease AF060479.1 This gene codes for x 2 1 an enzyme involved in the repair of AND / mismatch repair and recombination In the above table, x N represents the multiplication factor of the stimulation compared to the untreated skin. As demonstrated in Table 12 above, the mixture comprising an essential oil of immortelle, an aqueous extract of immortelle, an oily immortelle extract, an essential oil of myrtle and honey advantageously allows to stimulate the expression genes encoding proteoglycan 4 thus reducing lines and wrinkles. Indeed, proteoglycans form a fundamental substance of the highly hydrated dermis. These molecules are charged, they are extremely hydrophilic and attract a large amount of water. They are structures, of variable size, very stretched, which occupy a considerable volume. They play a very important role in the filling of spaces thus allowing cell migration. The lubricine can be described as a kind of biological lubricant. The mixture comprising an essential oil of immortelle, an aqueous extract of immortelle, an oily immortelle extract, an essential oil of myrtle and honey also advantageously makes it possible to stimulate the expression of genes coding for lumican which is a protelycane to organize the collagen fibers and their spacing. Thus, the mixture advantageously allows to fight against skin aging. In particular, the mixture makes it possible to obtain a firmer and more elastic skin. The mixture comprising an essential oil of immortelle, a 421-aqueous extract of immortelle, an oily immortelle extract, an essential oil of myrtle and honey also advantageously makes it possible to stimulate the expression of the genes encoding the SPDYA Speedy protein. x2 that intervenes in the regulation of the cell cycle, that is cell division. It plays a key role in controlling normal cell growth and inhibiting cell death. Thus the mixture comprising immortelle essential oil, aqueous immortelle extract, oily immortelle extract, myrtle essential oil and honey advantageously protects the skin cells from external aggressions, such as ultra-violet radiation. -violets. Finally, the mixture comprising an immortelle essential oil, an aqueous immortelle extract, an oily immortelle extract, an essential oil of myrtle and honey also advantageously makes it possible to stimulate the expression of the genes coding for the enzyme. Exonuclease 1 EX01 x2 which is involved in the removal of altered parts of the DNA to prepare its repair. Thus, the mixture advantageously stimulates the repair mechanisms of skin cells. As demonstrated in this example, especially Tables 12 and 13 above, the mixture comprising immortelle essential oil, aqueous immortelle extract, immortelle oily extract, myrtle essential oil and honey, allows a synergistic stimulation of genes allowing the reduction of wrinkles / fine lines, an increase in the smoothing of the skin, a reinforcement of the cutaneous barrier, an increase of the cutaneous firmness and elasticity and an increase of the cellular repair mechanisms. EXAMPLE 6 Test of Use and Efficacy of the Composition According to the Invention In order to validate the efficacy and the anti-aging action of the composition of the invention, 50 women aged between 40 and 70 (average age is 64.7 years) were recruited for this study to test the anti-wrinkle / anti-aging activity and the toning effect of the composition as well as the cosmetic qualities of this composition. As a result, the composition was applied daily for 28 days by 50 women on the face and neck under normal conditions of use. All these volunteers had wrinkles. In this example, the composition used was the composition 1 of example 1 above. This composition is referred to below as "Divine Cream". The method used is summarized in Table 13 below. Table 13: Dermatologist Investigator Clinical Evaluation Procedure Criterion (s) Area (s) Principle Kinetics Condition Assessed (s) of Sewing System Appearance / Contour 9-point scoring Volunteer JO Intensity eyes (1) (1 to 9 - seated in front of (global) visual reference) (3) Investigator and J28 wrinkles under controlled conditions Overall Appearance / Intensity of Wrinkles System Contour Depth the eye wrinkle (1) scoring in (crow's feet) 10 points (1 to 9 - visual) (2) Number of wrinkle system in 6 points (0 to 5 - visual) (2) In table 13 ci above: (1) indicates: left or right side according to randomization (2) Double entry IEC visual scale (number of wrinkles in 6 points and depth of wrinkles in 10 points), and (3) 1 = no / little and 9 = much / very Table 14 Clinical evaluation procedure by dermatologist investigator Criterion (ies) Primary area (s) eg Kinetic Condition Assessed (s) of scoring Ptosis of lower part of face Scale Volunteer OO the oval of the photographic seated in front of (reference) and J28 face in the Investigator 6 grades (0 to 5 sub - visual), conditions page 57 of Controlled atlas of skin aging (2) Smooth aspect Full face Skin system 9-point scoring (1 to 9 - visual) (5) Skin radiance Number of scour spot system in 7 pigmentation points (0 to 6 - visual) (3) Intensity of System Spot color pigmentation scouring in 9 the spot of selected points pigmentation by (1 to 9 - visual) the investigator Dermatologist, '' spot of (5) Size of the Measure using pigmentation of a slider (mm) Firmness of Cheek (1) System of skin scoring in 9 points (1 to 9 - touch, skin density to the pinch) (5) Skin tone In table 14 above: (1) indicates Right or left side according to randomization (2) Atlas of aging c utane, Vol.1. European population, R. Bazin & E. Doublet [9]. (3) Scoring system representing the percentage of the pigmented part of the face: 0: No pigmentation spot 4: 50 to 69% 1: <10% 5: 70 to 89% 2: 10 to 29% 6: 90 to 100 % 3: 30 to 49% (4) At OJ, selection by the Dermatologist Investigator of a significant pigmentation spot (color, size). In order to ensure a good repositioning at each time of the study, a transparent plastic mask is made. (5) 1 = no / little and 9 = much / very In terms of anti-wrinkle performance, the overall results are shown in Table 15 below. Table 15: Antiaging properties of the composition Averages and standard deviations (Sd) Probability Percentage of variation Delta p: effect # time Wilcoxon test N OJ J28 Initial value Appearance / intensity 48 6.10 + 1.22 5.96 +1.32 0.063 -2% +0.56 overall wrinkles Appearance / intensity 48 5.56 + 0.82 5.13 + 0.98 <0.001 -8% -0.43 overall wrinkles Depth of main wrinkle 48 7.04 + 1.16 6.67 + 1.33 <0.001 _5% -0.37 Number of wrinkles 48 4.27 ± 34.20 ± 0.031 -2% 0.81 0.87 -0.07 Ptosis of the oval of AQ 4.20 ± 4.00 ± <0001 -5% -0.20 face -ru 0.56 0.59 Smooth appearance of the 48 4.27 ± 5.10 ± <0.001 + 19% +1,19 skin (visual 1.01 0.97 Intensity of the 48 5.56 ± 4'48 ± <0.001 -19% -1.08 color of the spot 0.80 1.13 pigmentation ° (visual) Spot size of 48 7.08 ± 6.92 ± 0.016 -2% -0.16 pigmentation ° 2.97 2.92 Skin firmness zin 4.40 ± 5.08 ± <0.001 + 15% +0 , 68 (tactile) `- '0.74 0.99 Skin density zin 4.54 ± 5.15 ± <0.001 + 13% +0.61 (tactile) w 0.99 1.03 Tonicity of skin 48 4.52 ± 5.33 ± <0.001 + 18% + 0.81 (tactile) to J 0.85 0.93 In the table above: "N" corresponds to the number of subjects "OJ" corresponds to at the first day of treatment, "D28" is the 28th day of treatment, "#" is the change from baseline, "Delta" is the difference (s) between the time averages. considered evaluation (s) and the initial values, "p" corresponds to the statistical validity threshold, a value of p <0.05 is very significant; a value of p is close to the significance when 0.05 5 p 0.10; a value of p 0.10 is not significant. The statistical test used for this analysis is a Wilcoxon test. In terms of performance, the composition was recognized as being very effective by users as shown in Table 16. After 1 and 4 weeks of use, the effects of the cosmetic composition according to the invention were also validated by the volunteers. Table 16: Overall Judgment of the Composition A APPLICATION Texture penetrates quickly 96% The product leaves a non-greasy finish 92% The product leaves a non-sticky finish 92% IMMEDIATE RESULTS The skin is soft 98% The skin is comfortable 98% The skin is intensely nourished 94% The skin reveals a new softness 96% The skin has a silky touch 96% The texture of the skin appears improved 81% The skin is velvety 90% The skin regains its radiance 71% The complexion is fresher 85% The complexion is more uniform 67% The complexion is more rested 75% The skin is more radiant 65% The skin is sublimated 56% The skin is plumper 52% The skin looks smoother 75% The skin is come back 50% The skin is firmer 52% The skin is revitalized 65% The skin is more toned 63% AFTER 1 WEEK The skin is intensely nourished 94% The skin reveals a new softness 98% The skin has a silky touch 98% The texture of the skin skin appears improved 94% Skin is velvety 92% Skin found e are radiance 85% The complexion is fresher 88% The complexion is more harmonious 85% The complexion is more uniform 83% The complexion is unified 85% The complexion is clarified 92% The skin is rid of its dull veil 85% The skin is more radiant 85% The skin texture looks refined 83% The skin is more beautiful 83% The skin is sublimated 67% The skin is stretched 79% The skin is plumper 71% The skin is plumped 69% The skin is smoother 81% The wrinkles are visibly smoothed 71% The wrinkles are filled 65% The deep wrinkles seem less pronounced 65% The skin regains its elasticity 79% The skin is firmer 79% The skin regains its tone 77% The skin is more dense 71 % The skin regains consistency 65% The skin is revitalized 88% The skin is like restructured 63% The substance of your skin is improved 83% The firmness of the skin is restored 73% The oval of the face is better drawn 60% The contours of the face are better defined 63% The volumes of the face are more harmonious 65% The strokes are enhanced 60% The strokes are remodeled 60% The skin behaves like a young skin 50% The skin seems to be reprogrammed 58% The skin regains its dynamism 65% Your skin reacts again like a young skin 50 % Your skin seems to recover characteristics 50% of a young skin The skin reveals a new youth 50% The skin looks younger 67% The product makes it possible to fight against the effects of time 75% You are told that you do not make your skin Age 63% Touching, you feel the difference 81% You have noticed the improvement of the state of your skin, 79% in the sight as in the touch You did not expect such a result 60% The speed of results you Impressed 65% You have noticed a clear improvement in the overall appearance of your skin 81% AFTER 4 WEEKS The skin is intensely nourished 92% The skin reveals a new softness 96% The skin has a silky touch 96% The texture of the skin skin looks improved 92% The pea u is velvety 96% The skin is radiant 92% The complexion is fresher 85% The complexion is more harmonious 85% The complexion is more uniform 79% The complexion is unified 79% The complexion is clarified 85% The pigmentary spots are blurred 60% The skin is rid of its dull veil 81% The skin is more radiant 79% The skin is sublimated 69% The skin is tightened 75% The skin is plumper 67% The skin is plumped 69% The skin is smoother 88 % The wrinkles are visibly smoothed 71% The wrinkles are filled 67% The deep wrinkles seem less pronounced 58% The skin regains its elasticity 83% The skin seems regenerated 85% The skin regains its tone 79% The skin is more dense 73% La skin regains consistency 77% Skin is revitalized 85% Skin is firmer 75% Skin is restructured 71% Skin substance is improved 83% Skin firmness is restored 73% The oval of the face is better drawn 60% The contours of the face are better d Finished 63% The volumes of the face are more harmonious 65% The strokes are enhanced 60% The strokes are like remodeled 60% The skin behaves like a young skin 50% The skin seems to be reprogrammed 58% The skin regains its dynamism 65% Your skin reacts again like a young skin 50% Your skin seems to find the characteristics of a young skin 50% The skin reveals a new youth 50% The skin looks younger 67% The product helps to fight against the effects of time 75% You are told that you are not your age 63% At the touch, you feel the difference 81 ° / 0 You have noticed the improvement of the state of your skin, 79 ° / o in the sight as in the touch You do not you do not expect such a result 60% The speed of the results impressed you 65% The composition was therefore recognized as being very efficient by the users. After 4 weeks of use, the claimed actions on the active complex are validated by the volunteers as shown in Table 16 above. The composition was therefore recognized as being very effective by users after 4 weeks of applications. The composition comprising the mixture of the aqueous extract, oily and essential oil of immortelle and the essential oil of myrtle has therefore been recognized as being very effective by the users after 4 weeks of applications. EXAMPLE 7 Test of the Composition According to the Invention In order to validate the effectiveness and the moisturizing action of a composition according to the invention, 10 women between 26 and 70 years old (average age is 56) have were recruited for this study to test the effectiveness and moisturizing action at 24 hours of the composition as well as the cosmetic qualities of this composition. As a result, the composition was applied to one of the two zones of 20 cm 2 delimited on the forearms of the volunteers. The quantity applied was 2 mg / cm 2, ie 40 mg. The area on which no composition was applied served as control. A measurement of the electrical capacitance of the skin at three different locations of the skin was made by a corneometer, namely the CN 825 corneometer (Courage + Khazaka electronic GmbH). Measurements were made at the application (TO), 2 hours (T2), 8 hours (T8) and 24 hours (T24) after application. The moisturizing effect of the tested composition was evaluated by calculating the average variation in capacitance according to the following formula. You noted a marked improvement in the overall appearance of your skin. 81% Mean variation = [((Tx hours - T0) / TO) x100] processed - [((Tx hours - T0) / T0) x100] control where x is the time after application. Table 17 shows the measurements of capacitance at different times. Table 17: Capacitance Measurement Control Zone Treated Area Average Deviation Mean Standard Deviation Standard Initial Measurement (TO) 32.1 4.6 33.1 3.1 Measurement at 2 hours 32.1 4.0 46.0 5.9 (T2) Measurement at 8 o'clock 32.0 4.7 45.7 8.7 (T8) 24-hour measurement 32.3 4.5 39.5 4.1 (T24) Table 18 shows the difference in electrical capacitance compared to the initial measurement.

Table 18: Variation of capacitance over time Control area Treated area Average Deviation Mean Standard deviation Standard Measurement at 2 hours 0.0 2.1 12.9 4.7 (T2) - initial measurement (TO) Measurement at 8 o'clock -0.1 1.9 12.6 7.2 (T8) - initial measurement (TO) 24-hour measurement 0.2 1.9 6.4 3.6 (T24) - initial measurement (TO) The results obtained showed a statistical increase of 39% in hydration at 2 hours, 38% at 8 hours and 19% at 24 hours.

As demonstrated in this example, the composition according to the invention also makes it possible to have a moisturizing effect on the epidermis, in particular a moisturizing effect for 24 hours after application. EXAMPLE 8 Test of Use and Efficacy of a Composition and a Combination of Compositions According to the Invention In order to validate the effectiveness of a composition of the invention or a combination of compositions according to the invention 54 women aged between 41 and 67 years (mean age 57 years) were recruited for this study in order to test the cosmetic qualities of a composition according to the invention or a mixture of compositions according to the invention. the invention. In this example, the compositions used were composition 1 of example 1 and composition 1 of example 3 above. These compositions are respectively referred to below as "Divine Cream" and "Divine Extract". As a result, Divine Cream or the combination "Divine Cream" and "Divine Extract" were applied independently 2 times daily for 28 days by the 54 women half of the face under normal conditions of use. The volunteers had wrinkles / fine lines / spots or relaxed skin. In terms of performance, the composition has been recognized as having an anti-aging and cosmetically effective effect by the users as shown in Table 19: Table 19: Texture and perfume The combination of products leaves a non-tacky finish 1000 / The combination of products does not leave any lint on the skin 94% Cream Combination only cream extract At the application The skin seems nourished 94% 93% The skin is comfortable 98% 96% The complexion is illuminated 76% 81% The wrinkles 54% 61% The skin appears to be plumped 63% 67% The skin looks visibly younger 61% 61% After 7 days The complexion is radiant 83% 83% The wrinkles seem smooth 72% 80% The skin is plump 61% 70% The skin is firmer 74% 83% The oval of the face is redrawn 54% 63% After 4 weeks The texture of the skin appears visibly 93% 94% improved The complexion is bright 89% 89% The spots are blurred 54 % 63% The spots are corrected 43% 50% The complexion is standardized 80% 87% The skin is more beautiful 89% 93% The complexion is more harmonious 89% 89% The skin is smoother 89% 87% The wrinkles are reduced 70% 80% The wrinkles are filled 50% 59% The wrinkles are corrected 52% 63% Expression wrinkles appear reduced 67% 74% Deep wrinkles appear less pronounced 56% 63% Skin is firmer 87 %% 91% Skin is denser 80% 85% Skin is tightened 74% 87% overall facial architecture is like 52 ° / 0 63 ° / 0 restored The skin looks plumped, plump 78% 80% The contours are like remodeled 52% 67% The facial volumes are more harmonious 80% 89% The oval of the face redraws 76% 81% Skin slackening is reduced 83% 83% Skin is sublimated, more beautiful 67% 72% Skin is like regenerated 72% 80% Serum seems to have doubled anti-aging efficacy 80% Serum seems to have increased anti-aging efficacy 65% Serum has increased anti-aging efficacy 83% Serum has strengthened the effectiveness firmness 83% Serum has strengthened 80% Efficiency Serum enhanced stain-free efficacy 61% The combination resulted in faster anti-aging results 76% The combination resulted in more visible anti-aging results 74 % The serum boosts the global anti-aging corrective action 80% The skin is more beautiful with the 83% combination As demonstrated in this example, the composition according to the invention or a mixture of compositions according to the invention makes it possible to have an anti-aging effect, a lightening effect of the complexion, and an anti-cutaneous effect, while moisturizing the epidermis.

EXAMPLE 9 Test of Use and Efficacy of a Composition According to the Invention In order to validate the effectiveness and the anti-aging action of the composition of the invention, 51 women between 40 and 70 years old (average age is 57 years) were recruited for this study in order to test the anti-wrinkle / anti-aging activity and the tolerance of the composition as well as the cosmetic qualities of this composition. As a result, the composition was applied twice daily for 56 days to the face and neck, with the exception of the eye contour, under normal conditions of use.

All these volunteers had wrinkles / fine lines. The composition used was Composition 1 of Table 6. This composition is referred to below as "Divine Extract". For the effectiveness of the composition, an evaluation was carried out on the first day, the 28th day and the 56th day according to the following scenario: - Wrinkles or fine lines at the crow's feet: 0 = no wrinkles , 6 = many wrinkles - Black dots: 0 = wide, 9 = small - Intensity of the color of the spots: 0 = black, 9 = clear - Uniqueness of the complexion: 0 = not uniform, 9 uniform - Skin sagging o 0 = non-dense skin, 9 = dense skin o 0 = non-firm skin, 9 = firm skin o 0 = non-toned skin, 9 = tonic skin - main wrinkle 6 = deep, 0 = thin line A significant improvement in the uniqueness of the complexion : + 19.18% after 28 days, + 29.17% after 56 days, the firmness of the skin: + 17.05% after 28 days and skin tone; + 17 In terms of performance, the composition was found to be effective by users as shown in Table 20: Table 20: Overall Judgment of Texture and Fragrance Composition m The texture is fresh 100% The texture is velvety 98% The texture is melting 100% The fragrance is natural 96% At the application The product penetrates quickly 100% The product leaves a non-sticky finish 92% The product leaves a finish no 100% fat The application gives a feeling of freshness 100% Immediate effect The skin is velvety 100% The skin is radiant 96% The complexion is illuminated 94% The wrinkles seem to fade 78% The skin seems smoothed The skin seems retightened 88% The skin is plumped 90% The skin is filled 86% The skin is sublimed 86% The skin is like processed 80% The damage caused by the time seems 80% attenuated After 7 days The appearance of the skin seems visibly improved 88% The complexion is radiant 90% The spots seem to fade 72% The complexion seems standardized 90% The complexion finds brightness and transparency 88% The wrinkles are faded 82% The wrinkles seem reduced 76% The skin seems firmed 86% The skin is rebounded 84% The architectu overall face is like restored 78% The contours of the face are as redefined 80% The oval of the face is redrawn 78% The skin is sublimated 82% The skin appears to be metamorphosed 78% The skin is more beautiful 94 ° / 0 La skin looks younger 84% After 4 weeks The skin is velvety 100% The complexion is brighter 98% The spots are blurred 92% The complexion is unified 96% The complexion regains brightness and transparency 94% Wrinkles seem reduced 96% Wrinkles 92% The skin looks firmer 98% The skin is more dense 98% The sagging skin is reduced 96% The overall architecture of the face is as restored 94% The finesse of the features seems redefined 94% The oval redraws on the face 90% The skin appears to be metamorphosed 94% A new impulse of youth seems to come from the very heart of your skin 94% The whole of the signs of the age is attenuated The skin looks younger 96% After 8 weeks The complexion is bright 100% The stain 84% The complexion is unified 94% The complexion is more harmonious The complexion regains luminosity and transparency 92% The wrinkles are corrected 90% The wrinkles of expression seem reduced 92% The deep wrinkles appear reduced 84% The skin is more 96% The skin is more dense 92% The overall architecture of the face is as restored 90% The contours of the face are as redefined 90% The contours are like remodeled 88% The oval is redrawn on the face 88% The skin is sublimated 90% The skin appears to be metamorphosed 86% The skin appears younger 92% The skin appears plumped 92% The cutaneous relaxation is reduced 90% A new impulse of youth comes from the very heart of your skin 82% The skin is visibly younger 86% You are told that you are not your age 88% At touch, you feel the difference 96% You have become very addicted to the product 82% You feel younger 86% You have noticed a clear improvement of appearance As shown in this example, the composition has therefore been recognized as being very effective by the users. After 7 days, 4 and 8 weeks of use, the claimed actions on the active complex were validated by the volunteers as shown in Table 20 above. Moreover, the moisturizing effect of composition 1 of Table 6 over 24 hours was also tested. 12 women between 33 and 69 years old with dry skin and with a corneometric index equal to 25.03 u.a. were recruited. As a result, 0.07 ml of composition was applied to a surface of 35 cm 2 of the skin. A comparison was made between the skin surface on which the composition was applied vs. a similar skin surface without application of the composition. A measurement of the electrical capacitance of the skin at three different locations on the skin was performed by a corneometer, the CN 825 corneometer (Courage + Khazaka electronic GmbH).

Measurements were made at the application (TO), 2 hours (T2), 8 hours (T8) and 24 hours (T24) after application. The results of variation of hydration as a function of time and of the treated or non-treated zone are shown in Table 21 below: Table 21: variation of hydration as a function of time 2 hours 4 hours 8 hours 24 hours Variation on the zone + 56.67% + 60.47% + 36.86% + 9.30% treated Variation on the zone 0.11% 2.11% -1.66% -2.55% untreated Variation between the treated zone and the untreated zone 52.56% 58.36% 38.56% 11.86% As demonstrated in this example, a composition according to the invention also makes it possible to significantly increase the hydration of the skin 24 hours compared to untreated skin.

Example 10: Use test of a composition according to the invention In order to validate the effectiveness and the anti-aging action of the composition of the invention, 52 women aged between 41 and 70 years (average age is 62 years old) were recruited for this study in order to test the anti-wrinkle / anti-aging activity and the tolerance of the composition as well as the cosmetic qualities of this composition. As a result, the composition was applied twice a day for 28 days to the face and neck using a cotton pad, except for the eye area, under normal conditions of use. All these volunteers had wrinkles / fine lines.

The composition used was composition 1 of Table 8. This composition is referred to below as "Divine lotion".

In terms of cosmetic quality and efficacy, the composition has been recognized as being effective by users as shown in Table 22: Table 22: Overall Judgment of Composition Texture and Fragrance Texture is Fresh 98% Texture has a wraparound freshness 96% The texture offers a comfort veil 96% The perfume is pleasant 92% The perfume is natural 94% The texture is rich 96% At the application The product is easily applied 100% The product penetrates quickly 100 % The product leaves a non-sticky finish 94% The product leaves a non-greasy finish 100% The product delivers instant intense comfort and well-being 90% The application provides a feeling of freshness 94% The skin is balanced 96% La skin is refreshed 96% Skin is toned 96% Skin is softer 98% Skin feels hydrated 92% Skin regains a delicious feeling of suppleness 94% Skin tone looks fresher 96% Skin feels more energetic 92% Pea u looks brighter 90% Complexion glows 92% Skin texture looks smoother 96% Skin looks smoother 96% Hair looks visibly smooth 90% Skin looks plumped 87% Skin texture looks visibly improved 92% The skin seems better prepared to receive the other care you use 98% The skin is ideally prepared to receive the benefits of your daily care 96% A 7 days The balance of the skin is maintained 100% The skin is more velvety 94% The skin is soft and hydrated 94c / 0 The skin is hydrated 98% The skin is intensely hydrated 79'Y ° The skin is fortified 90% The skin is supple 96% The skin offers an incredible sensation of suppleness 88% The skin is more toned 94 ° / 0 The strokes are smooth 90% The complexion is brighter 85% The complexion seems unified 92% The complexion is more harmonious 88% The skin texture is refined 90% The skin is smoother 94% The skin appears refreshed and relipidée 79% The skin seems firmer 83% The skin is tightened 81% The skin looks more beautiful 85% The skin is sublimated 75% The skin regains its dynamism 83% The skin is ideally prepared to receive the benefits of your daily care 94% The product optimizes the benefits of others care that you use 92% At 4 weeks The balance of the skin is preserved 96% The skin is infinitely velvety 92% The skin is infinitely soft 92% The skin is intensely hydrated 85% The skin is more resistant 92% The complexion is radiant 88% The complexion is more harmonious 83% The complexion finds brightness and transparency 85% The skin radiates youth 79% The skin texture is refined 87% The appearance of the pores is reduced 87% The skin is smoother 94% The complexion is more toned 90% The strokes are visibly smoothed 88% The skin appears refreshed and relipidated 79% The skin is tightened 81% The skin regains firmness and elasticity 77% The oval is redrawn on the face 67% The skin is sublimated 79 % The skin looks metamorphos 62% The skin is more beautiful 75% The skin looks younger 71% A pulse seems to come from the very heart of the skin 63% The quality of the skin is like transformed 69% The skin appears regenerated from the inside 65% L all signs of age seem attenuated 65% As demonstrated in this example and in table 22, the composition tested was therefore recognized as being very effective by the users. After 7 days and 4 weeks of use, the claimed actions on the active complex were validated by volunteers as shown in Table 22 above.

References 1. Koblenzer CS. Psychological aspects of aging and the skin. Dermatol Clinics 1996; 14: 171-7. 2. Seitz G, Bonin M, Fuchs J et al. Inhibition of glutathione-S-transferase as a treatment strategy for multidrug resistance in childhood rhabdomyosarcoma. International Journal of Oncology 2010 36: 491-500 3. Pierard GE. The when of climacteric skin aging. Dermatology 1996; 193: 273-4 4. Petersen MJ, Hansen C, Craig S. Ultraviolet A irradiation stimulates collagenase production in cultured human fibroblasts. J Invest Dermatol 1992; 99: 440-4 5. West MD. The cellular and molecular biology of skin aging. Arch Dermatol 1994; 130: 87-95 6. Harvell JD, Maibach HI. Percutaneous absorption and inflammation in aged skin: a review. J Am Acad Dermatol 1994; 31: 1015-21. 7. Grove GL, Kligman AM. Age-associated changes in human epidermal cell renewal. J Gerontol 38: 137-42 8. Tim Mosmann. Rapid colorimetric assay for cell growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods Volume 65, Issues 1-2, 16 December 1983 Pages 55-63 9. Atlas of Skin Aging, Vol.1. European population, R. Bazin & E. Doublet.

Claims (17)

REVENDICATIONS1. Cosmetic composition comprising an aqueous extract of immortelle, an oily extract of immortelle, an essential oil of immortelle and an essential oil of myrtle. 2. Composition according to claim 1 wherein the concentration of the aqueous extract of immortelle is from 0.01% to 10% by weight relative to the total weight of the composition. 3. Composition according to claim 1 or 2 wherein the concentration of oily extract of immortelle is from 0.001% to 5% by weight relative to the total weight of the composition. 4. Composition according to any one of claims 1 to 3 wherein the essential oil concentration of immortelle is from 0.001% to 2% by weight relative to the total weight of the composition. 5. Composition according to any one of claims 1 to 4, wherein the concentration of essential oil of myrtle is from 0.001% to 1% by weight relative to the total weight of the composition. 6. Composition according to any one of claims 1 to 5, wherein the composition further comprises honey. 7. Composition according to claim 6, wherein the honey is spring honey from Corsica. 8. The composition of claim 6 or 7 wherein the honey concentration is 0.01 to 15% by weight based on the total weight of the composition. 9. Composition according to any one of claims 1 to 7, wherein the composition further comprises a hydroglycerine extract of clover water. 10. Composition according to claim 9, wherein the concentration of the hydroglycerine extract of water clover is 0.01 to 15% by weight relative to the total weight of the composition. 11. Composition according to any one of claims 1 to 10, wherein the composition further comprises a hyaluronic acid derivative. 12. Composition according to claim 11, wherein the concentration of the hyaluronic acid derivative is from 0.001 to 3% by weight relative to the total weight of the composition. 13. A composition according to any one of claims 1 to 12, wherein the composition further comprises an extract of Bellis Perennis. 14. The composition of claim 13, wherein the concentration of the Bellis Perennis extract is 0.01 to 15% by weight relative to the total weight of the composition. 15. Composition according to any one of claims 1 to 14, wherein the composition further comprises at least one oil selected from evening primrose, borage, echium and / or camelina vegetable oil. 16. Composition according to any one of claims 1 to 15, said composition being in a form selected from the group comprising a cream, a milk, a lotion, a gel, a serum, an ointment. 17. A method of anti-aging cosmetic care comprising a step of applying to the skin of a cosmetic composition as defined in any one of claims 1 to 16.