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ES2663955T3 - Evaluación de la susceptibilidad a antibióticos utilizando sondas para arn prerribosómico - Google Patents

Evaluación de la susceptibilidad a antibióticos utilizando sondas para arn prerribosómico Download PDF

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Publication number
ES2663955T3
ES2663955T3 ES13784842T ES13784842T ES2663955T3 ES 2663955 T3 ES2663955 T3 ES 2663955T3 ES 13784842 T ES13784842 T ES 13784842T ES 13784842 T ES13784842 T ES 13784842T ES 2663955 T3 ES2663955 T3 ES 2663955T3
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ES
Spain
Prior art keywords
target sequence
probe
probes
sample
pair
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES13784842T
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English (en)
Inventor
David Haake
Colin HALFORD
Jane T. BABBITT
Bernard M. Churchill
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Veterans Affairs
University of California Berkeley
University of California San Diego UCSD
Original Assignee
US Department of Veterans Affairs
University of California Berkeley
University of California San Diego UCSD
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Application filed by US Department of Veterans Affairs, University of California Berkeley, University of California San Diego UCSD filed Critical US Department of Veterans Affairs
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Publication of ES2663955T3 publication Critical patent/ES2663955T3/es
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un método para determinar si una muestra de bacterias es susceptible a un agente antibiótico, comprendiendo el método los pasos de: (a) (a) poner en contacto un espécimen obtenido a partir de la muestra con una sonda o un par de sondas de oligonucleótidos en ausencia del agente, en donde la sonda o el par de sondas hibrida específicamente a una secuencia diana sobre la longitud completa de la secuencia diana, en donde la secuencia diana comprende 25-35 nucleótidos contiguos de ARN ribosómico (rARN) bacteriano que abarca un sitio de empalme entre una cola de ARN prerribosómico (prARN) y ARN ribosómico maduro (mrARN) (b) (b) poner en contacto un espécimen obtenido a partir de la muestra con la sonda o par de sondas en presencia del agente antibiótico; (c) (c) detectar las cantidades relativas de hibridación de la sonda a la secuencia diana en los especímenes de (a) y (b); (d) (d) identificar la muestra como susceptible al tratamiento con antibiótico si la cantidad de hibridación de la sonda a la secuencia diana en el paso (b) se reduce al menos un 80% con relación a la cantidad de hibridación de la sonda a la secuencia diana en el paso (a).
ES13784842T 2012-05-04 2013-05-03 Evaluación de la susceptibilidad a antibióticos utilizando sondas para arn prerribosómico Active ES2663955T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261643009P 2012-05-04 2012-05-04
PCT/US2013/039574 WO2013166460A1 (en) 2012-05-04 2013-05-03 Antibiotic susceptibility testing using probes for preribosomal rna

Publications (1)

Publication Number Publication Date
ES2663955T3 true ES2663955T3 (es) 2018-04-17

Family

ID=49514929

Family Applications (1)

Application Number Title Priority Date Filing Date
ES13784842T Active ES2663955T3 (es) 2012-05-04 2013-05-03 Evaluación de la susceptibilidad a antibióticos utilizando sondas para arn prerribosómico

Country Status (6)

Country Link
US (1) US10370728B2 (es)
EP (1) EP2844762B1 (es)
DK (1) DK2844762T3 (es)
ES (1) ES2663955T3 (es)
PL (1) PL2844762T3 (es)
WO (1) WO2013166460A1 (es)

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CN105277694B (zh) * 2014-08-18 2017-01-18 董俊 人a群链球菌量子点免疫层析检测卡及其制备方法和应用
CN104374922A (zh) * 2014-11-26 2015-02-25 绍兴康知生物科技有限公司 一种检测氯霉素的蛋白制备方法
WO2018058078A1 (en) 2016-09-23 2018-03-29 California Institute Of Technology Digital quantification of dna replication and/or chromosome segregation based determination of antimicrobial susceptibility
SI3551293T1 (sl) * 2016-12-06 2022-06-30 Microbedx, Inc. RNAZA za izboljšano odkrivanje mikrobov in testiranje protimikrobne dovzetnosti
US11053532B2 (en) 2017-04-19 2021-07-06 CAP Diagnostics, LLC Methods for treating polymicrobial infections
NZ759292A (en) * 2017-04-19 2022-11-25 Cap Diagnostics Llc Dba Pathnostics Assay for the comprehensive identification of antibiotic sensitivity
US20200263224A1 (en) * 2017-08-18 2020-08-20 Microbedx, Inc. Methods for Antimicrobial Susceptibility Testing
US11827944B2 (en) 2017-10-11 2023-11-28 California Institute Of Technology Antibiotic susceptibility of microorganisms and related compositions, methods and systems
US12448655B2 (en) 2017-10-11 2025-10-21 California Institute Of Technology Antibiotic susceptibility of microorganisms and related methods and systems
US20210246489A1 (en) * 2018-05-14 2021-08-12 Microbedx, Inc. Methods for estimating microbial density in specimens by measurement of ribosomal rna
US20230183818A1 (en) * 2018-08-01 2023-06-15 California Institute Of Technology Antibiotic susceptibility of microorganisms and related markers, compositions, methods and systems
WO2021021782A1 (en) * 2019-07-29 2021-02-04 Microbedx Inc. Method for antibiotic susceptibility testing of blood samples

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FI843529A0 (fi) 1983-01-10 1984-09-10 Gen Probe Inc Foerfarande foer detektering, identifiering och kvantitering av organismer och virus.
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US5712095A (en) 1994-06-16 1998-01-27 Becton Dickinson And Company Rapid and sensitive detection of antibiotic-resistant mycobacteria using oligonucleotide probes specific for ribosomal RNA precursors
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US6391558B1 (en) 1997-03-18 2002-05-21 Andcare, Inc. Electrochemical detection of nucleic acid sequences
DE19731292A1 (de) 1997-07-21 1999-01-28 Biotecon Ges Fuer Biotechnologische Entwicklung & Consulting Mbh Nucleinsäuremolekül, Kit und Verwendung
GB9805918D0 (en) * 1998-03-19 1998-05-13 Nycomed Amersham Plc Sequencing by hybridisation
CA2407973C (en) 2000-05-03 2011-06-07 Jen-Jr Gau Biological identification system with integrated sensor chip
DE60333032D1 (de) 2002-10-23 2010-07-29 Virxsys Corp Screening-verfahren zur identifizierung wirksamer prä-trans-spleissmoleküle
AU2004265570B2 (en) * 2003-05-13 2009-01-08 Gen-Probe Incorporated Method and kit for identifying antibiotic-resistant microorganisms
US7943754B2 (en) * 2004-04-02 2011-05-17 Rosetta-Genomics Bioinformatically detectable group of novel regulatory bacterial and bacterial associated oligonucleotides and uses thereof
US7763426B2 (en) * 2004-11-01 2010-07-27 The Regents Of The University Of California Probes and methods for detection of Escheridia coli and antibiotic resistance
DE602007005235D1 (en) * 2006-03-31 2010-04-22 Eidgenoess Tech Hochschule Screening-test für ribosomale antibiotika
WO2012027302A2 (en) 2010-08-21 2012-03-01 The Regents Of The University Of California Systems and methods for detecting antibiotic resistance
US9512474B2 (en) * 2011-09-28 2016-12-06 Lonza Walkersville, Inc. Molecular targets and methods for formulation screening and preservative efficacy testing

Also Published As

Publication number Publication date
EP2844762A1 (en) 2015-03-11
DK2844762T3 (en) 2018-03-26
PL2844762T3 (pl) 2018-07-31
US10370728B2 (en) 2019-08-06
EP2844762A4 (en) 2015-12-30
EP2844762B1 (en) 2017-12-20
US20150104789A1 (en) 2015-04-16
WO2013166460A1 (en) 2013-11-07

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