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EP3673035B1 - Propriétés d'entretien améliorées de textiles polyester ii - Google Patents

Propriétés d'entretien améliorées de textiles polyester ii

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Publication number
EP3673035B1
EP3673035B1 EP18758569.0A EP18758569A EP3673035B1 EP 3673035 B1 EP3673035 B1 EP 3673035B1 EP 18758569 A EP18758569 A EP 18758569A EP 3673035 B1 EP3673035 B1 EP 3673035B1
Authority
EP
European Patent Office
Prior art keywords
cutinase
agent
amino acid
textiles
detergent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP18758569.0A
Other languages
German (de)
English (en)
Other versions
EP3673035A1 (fr
Inventor
Nina Mussmann
Susanne Wieland
Daniela HERBST
Margret VAN LIER
Ines Baranski
Alexander Schulz
Boray TORUN
Christina SCHÖNDELING
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Henkel AG and Co KGaA
Original Assignee
Henkel AG and Co KGaA
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Publication date
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Publication of EP3673035A1 publication Critical patent/EP3673035A1/fr
Application granted granted Critical
Publication of EP3673035B1 publication Critical patent/EP3673035B1/fr
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
    • C11D2111/10—Objects to be cleaned
    • C11D2111/12—Soft surfaces, e.g. textile

Definitions

  • the present invention lies in the field of enzyme technology, in particular the anti-pilling effect of enzymes, such as those used, for example, in washing or cleaning agents.
  • the invention relates to an agent, in particular a washing or cleaning agent, which contains a cutinase as defined herein.
  • the present invention relates to a method for cleaning textiles and the use of the agent according to the invention for removing soiling.
  • the invention is directed to the use of a cutinase for reducing pilling and graying in an agent, preferably a washing or cleaning agent.
  • Pilling is the formation of small balls or fuzz in fabrics. These small balls of fuzz occur particularly in short-fiber fabrics. Long-fiber and twisted fibers, on the other hand, pill less. Generally, these balls are caused by loose fibers in the fabric or fibers that have detached from the weave. Synthetic fibers, due to their smooth surface, are more prone to pilling than natural fibers because synthetic fibers can be released from the fabric more quickly than rough natural fibers. In wool fabrics, these fibers "felt” primarily due to mechanical friction, forming balls on the surface.
  • Pilling occurs particularly in areas subject to high mechanical stress, usually the shoulder and waistband areas. Due to the continuous thinning of the fabric, these stressed areas are particularly at risk of developing holes or even tearing. This unwanted pilling results in affected textiles being rejected and thrown away by consumers faster than would be necessary based on the functionality of the textile.
  • the cutinase used here shows rapid PET degradation at 50°C. It was found that the enzyme prevents pilling on new polyester textiles and, in combination with a cellulase, supports this effect on polyester/cotton blend textiles. Furthermore, it can reduce pills that have already formed, i.e., it can cause a so-called "renew" effect. Furthermore, the cutinase prevents the graying of white laundry and the fading/graying of colored laundry. Furthermore, the cutinase exhibits direct cleaning performance for stains on PET textiles. It was further found that all of these positive washing properties are achieved without significant damage to the fiber. Because the textiles look newer for longer, they are worn longer and replaced less frequently. This leads to a reduction in the CO2 footprint, as less polyester is used.
  • WO2015135757 discloses detergents containing PET esterases ⁇ 65% according to SEQ ID NO:1, which are suitable for reducing pilling in polyester-containing textiles. Therefore, the present invention is directed to a detergent or cleaning agent characterized in that it contains a cutinase that has at least 65% sequence identity with the amino acid sequence given in SEQ ID NO:1 over its entire length.
  • the present invention is directed to methods for cleaning textiles, characterized in that in at least one method step a The agent according to the invention is used.
  • the textiles are preferably polyester-containing textiles or are made of polyester.
  • the present invention is further directed to the use of a washing or cleaning agent as described herein, particularly preferably a liquid washing agent, for removing soiling.
  • a further aspect of the invention includes the use of the cutinase described herein for reducing pilling effects and/or increasing the anti-grey effect of a washing or cleaning agent, particularly preferably a liquid washing agent, wherein the agent contains the cutinase.
  • Cutinase (EC 3.1.1.74), also known as cutin hydrolase, is an enzyme belonging to the ⁇ / ⁇ -hydrolases family and hydrolyzes cutin. Cutinase is produced by some plant-pathogenic fungi and bacteria, among others (cutinase enables fungi to degrade the ester bond of cutin in the plant cuticle and thus penetrate the plant).
  • the cutinase is a cutinase which has at least 70% sequence identity with the amino acid sequence given in SEQ ID NO:1 over its entire length.
  • the cutinase contained in the agent according to the invention comprises the amino acid sequence given in SEQ ID NO:1 or consists essentially thereof or consists thereof.
  • the invention also encompasses cutinases derived from the amino acid sequence according to SEQ ID NO:1, for example by means of mutagenesis.
  • the invention also encompasses cutinases obtainable by expressing the nucleotide sequence according to SEQ ID NO:3.
  • the invention also comprises the nucleotide sequence according to SEQ ID NO:3 and nucleotide sequences which are at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5%, 98.8%, 99.0%, 99.2%, 99.4% or 99.6% identical to the nucleotide sequence given in SEQ ID NO:3 over its entire length. provided that the native sequence encoding LC cutinase is excluded.
  • the cutinase comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5%, 98.8%, 99.0%, 99.2%, 99.4% or 99.6% is identical or consists of such a sequence.
  • the agents according to the invention preferably contain the cutinase in an amount of 0.00001 - 1 wt.%, more preferably in an amount of 0.0001 - 0.5 wt.%, particularly preferably in an amount of 0.001 - 0.1 wt.%, in each case based on the active protein.
  • sequence comparison is based on the state-of-the-art and commonly used BLAST algorithm (see, for example, Altschul, SF, Gish, W., Miller, W., Myers, EW & Lipman, DJ (1990) "Basic local alignment search tool.” J Mol Biol 215:403-410 , and Altschul, Stephan F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Hheng Zhang, Webb Miller, and David J.
  • Lipman (1997): "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs"; Nucleic Acids Res., 25, pp.3389-3402 ) and is basically done by matching similar sequences of nucleotides or amino acids in the nucleic acid or amino acid sequences. A tabular assignment of the relevant positions is called an alignment.
  • Another algorithm available in the state of the art is the FASTA algorithm. Sequence comparisons (alignments), especially multiple sequence comparisons, are created using computer programs. For example, the Clustal series (see, for example, Chenna et al. (2003): Multiple sequence alignment with the Clustal series of programs.
  • T-Coffee see for example Notredame et al. (2000): T-Coffee: A novel method for multiple sequence alignments. J. Mol. Biol. 302, 205-217 ) or programs based on these programs or algorithms. Sequence comparisons (alignments) are also possible using the computer program Vector NTI ® Suite 10.3 (Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, California, USA) with the specified standard parameters; its AlignX module for sequence comparisons is based on ClustalW.
  • Such a comparison also allows a statement to be made about the similarity of the compared sequences. This is usually expressed as percent identity, i.e., the proportion of identical nucleotides or amino acid residues at the same or corresponding positions in an alignment.
  • the broader term "homology" in amino acid sequences includes conserved amino acid substitutions, i.e., amino acids with similar chemical activity, since these usually exert similar chemical activities within the protein. Therefore, the similarity of the compared sequences can also be expressed as percent homology or percent similarity.
  • Identity and/or homology statements can be made for entire polypeptides or genes, or just for individual regions. Homologous or identical regions of different nucleic acid or amino acid sequences are therefore defined by similarities in the sequences. Such regions often exhibit identical functions.
  • nucleic acid or amino acid sequence can be small and comprise only a few nucleotides or amino acids. Such small regions often perform essential functions for the overall activity of the protein. It can therefore be useful to refer sequence similarities only to individual, possibly small regions. Unless otherwise stated, details of identity or homology in the present application refer to the total length of the respective nucleic acid or amino acid sequence indicated.
  • the cutinase comprises an amino acid sequence that is at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5%, 98.8%, 99.0%, 99.2%, 99.4% or 99.6% homologous to the amino acid sequence given in SEQ ID NO:1 over its entire length.
  • the cutinase is characterized in that its anti-pilling performance is not significantly reduced compared to that of a cutinase comprising an amino acid sequence corresponding to the amino acid sequences given in SEQ ID NO:1, i.e., it has at least 70%, 75%, 80%, 85%, 90%, or 95% of the reference anti-pilling performance.
  • the anti-pilling performance can be determined in a washing system containing a detergent at a dosage of between 4.5 and 7.0 grams per liter of wash liquor and the cutinase, wherein the cutinases to be compared are used at the same concentration (based on active protein), and the anti-pilling performance is determined as described herein.
  • the washing process can be carried out for 60 minutes at a temperature of 60°C, and the water can have a water hardness of between 15.5 and 16.5°C (German hardness).
  • the concentration of cutinase in the detergent intended for this washing system is from 0.00001 to 1 wt.%, preferably from 0.0001 to 0.5 wt.%, particularly preferably from 0.001 to 0.1 wt.%, based on active, purified protein.
  • a preferred liquid detergent for such a washing system is composed as follows (all values in percent by weight): 4.4% alkylbenzenesulfonic acid, 5.6% anionic surfactants, 2.4% C12-C18 sodium salts of fatty acids, 4.4% nonionic surfactants, 0.2% phosphonates, 1.4% citric acid, 0.95% NaOH, 0.01% antifoam, 2% glycerin, 0.08% preservatives, 1% ethanol, 1.6% enzyme mix (protease, amylase, cellulase, mannase), and the remainder: demineralized water.
  • the preferred dosage of the liquid detergent is between 4.5 and 6.0 grams per liter of wash liquor, for example, 4.7, 4.9, or 5.9 grams per liter of wash liquor. Washing is preferably carried out in a pH range between pH 8 and pH 10.5, preferably between pH 8 and pH 9.
  • the anti-pilling performance is determined at 60°C using a liquid detergent as stated above, wherein the washing process is preferably carried out for 60 minutes.
  • Anti-pilling performance can be monitored through visual inspection.
  • a panel of testers assigns a score on a scale of 1-5 to the laundry being tested.
  • a score of 1 represents very heavily pilled laundry, while a score of 5 represents non-pilled laundry.
  • Proteins can be combined into groups of immunologically related proteins through reaction with an antiserum or a specific antibody.
  • the members of such a group are characterized by having the same antigenic determinant recognized by an antibody. They are therefore structurally so similar to one another that they are recognized by an antiserum or specific antibodies.
  • Another subject of the invention therefore relates to cutinases which are characterized by having at least one, and increasingly preferably two, three, or four, identical antigenic determinants with a cutinase used in an agent according to the invention. Due to their immunological similarities, such cutinases are structurally so similar to the cutinases used in the agents according to the invention that a similar function can be assumed.
  • cutinases used in the agents according to the invention may have further amino acid modifications, in particular amino acid substitutions, insertions, or deletions, compared to the cutinase described in SEQ ID NO:1.
  • Such cutinases are further developed, for example, through targeted genetic modification, i.e., through mutagenesis methods, and optimized for specific applications or with regard to specific properties (e.g., with regard to their catalytic activity, stability, etc.).
  • nucleic acids encoding the cutinases used can be incorporated into recombinant cultures and thus used to generate completely novel cutinases or other polypeptides.
  • the aim is to introduce targeted mutations such as substitutions, insertions, or deletions into the known molecules in order, for example, to improve the cleaning performance of enzymes according to the invention.
  • the surface charges and/or the isoelectric point of the molecules and thus their interactions with the substrate can be altered.
  • the net charge of the enzymes can be altered in order to influence substrate binding, particularly for use in detergents and cleaning agents.
  • one or more corresponding mutations can further increase the stability of the cutinase and thereby improve its cleaning performance.
  • Advantageous properties of individual mutations, e.g., individual substitutions can complement each other.
  • a cutinase that has already been optimized with regard to certain properties, for example, with regard to its activity and/or its anti-pilling performance, can therefore be further developed within the scope of the invention.
  • the invention therefore further relates to an agent containing a cutinase, which is characterized in that it is obtainable from a cutinase as described above as the starting molecule by single or multiple conservative amino acid substitution.
  • conservative amino acid substitution means the exchange (substitution) of one amino acid residue for another amino acid residue, whereby this exchange does not lead to a change in the polarity or charge at the position of the exchanged amino acid, e.g., the exchange of a non-polar amino acid residue for another non-polar amino acid residue.
  • the homology of the cutinases modified in this way to the cutinase with SEQ ID NO:1 is preferably as defined above.
  • the cutinase is characterized in that it is obtainable from a cutinase contained in an agent according to the invention as a starting molecule by fragmentation, deletion, insertion or substitution mutagenesis and comprises an amino acid sequence which has a length of at least 180, 190, 200, 210, 220, 230, 240, 245, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263 or 264 contiguous amino acids match the starting molecule.
  • the enzymes retain their hydrolytic activity even after mutagenesis, i.e., their hydrolytic activity corresponds at least to that of the starting enzyme, i.e., in a preferred embodiment, the hydrolytic activity is at least 80%, preferably at least 90% of the activity of the starting enzyme.
  • Other substitutions can also have beneficial effects. Both individual and multiple contiguous amino acids can be exchanged for other amino acids.
  • the cutinase may have one or more further amino acids at the N- or C-terminal end in addition to the sequence given in SEQ ID NO:1.
  • such N-terminal peptides may be the naturally occurring signal peptides for the cutinase or a single methionine residue.
  • the cutinase has, for example, the amino acid sequence given in SEQ ID NO:2. All embodiments disclosed above in the context of the mature cutinase according to SEQ ID NO:1 are also applicable to the cutinase with the sequence according to SEQ ID NO:2.
  • the invention relates to a washing or cleaning agent characterized by containing a cutinase as defined herein. Unless explicitly stated otherwise, all percentages given in connection with the compositions/agents described herein refer to wt. %, in each case based on the respective mixture/agent.
  • fatty acids or fatty alcohols or their derivatives represent branched or unbranched carboxylic acids or alcohols or their derivatives, preferably with 6 to 22 carbon atoms.
  • the oxo alcohols or their derivatives obtainable, for example, by the Roelen oxo synthesis can also be used accordingly.
  • alkaline earth metals are mentioned below as counterions for monovalent anions, this means that the alkaline earth metal is naturally present in only half the amount of the anion - sufficient to balance the charge.
  • washing or cleaning agents both concentrated and undiluted, for use on a commercial scale, in washing machines or for hand washing.
  • these include, for example, detergents for textiles, carpets, or natural fibers, for which the term "detergent" is used.
  • the washing and cleaning agents within the scope of the invention also include washing aids that are added to the actual washing agent during manual or machine washing to achieve an additional effect.
  • washing and cleaning agents within the scope of the invention also include textile pre- and post-treatment agents, i.e.
  • agents with which the laundry is brought into contact before the actual washing for example, to dissolve stubborn soils
  • agents that impart further desirable properties to the laundry such as a pleasant feel, resistance to creasing, or low static charge, in a step following the actual textile washing.
  • Fabric softeners are included among the latter agents.
  • the washing or cleaning agents according to the invention which can be in the form of powdered solids, in compacted particle form, as homogeneous solutions, gels, or suspensions, can contain, in addition to the cutinase described above, all known ingredients customary in such agents, with at least one further ingredient preferably being present in the agent.
  • the agents according to the invention can contain, in particular, surfactants, builders, bleaching agents, in particular peroxygen compounds, or bleach activators. They can also contain water-miscible organic solvents, other enzymes, sequestering agents, electrolytes, pH regulators, and/or other auxiliaries such as optical brighteners, graying inhibitors, foam regulators, dyes, and fragrances, as well as combinations thereof.
  • a combination of the agent according to the invention with one or more other ingredients is advantageous, since such an agent, in preferred embodiments according to the invention, exhibits improved cleaning performance due to resulting synergisms.
  • Such synergism can be achieved in particular by combining the agent according to the invention with a surfactant and/or a builder and/or a peroxygen compound and/or a bleach activator.
  • the agents according to the invention preferably also contain at least one compound from the class of surfactants, in particular selected from anionic and non-ionic, but also cationic, zwitterionic or amphoteric surfactants.
  • Suitable surfactants are, for example, anionic surfactants of the formula (I) R-SO 3 - Y + (I).
  • R represents a linear or branched unsubstituted alkylaryl radical.
  • Y represents a monovalent cation or the nth part of an n-valent cation.
  • Alkali metal ions are preferred, including Na + or K + , with Na + being most preferred.
  • Further cations Y + can be selected from NH4 + , 1 ⁇ 2Zn2 + , 1 ⁇ 2Mg2 + , 1 ⁇ 2Ca2 + , 1 ⁇ 2Mn2 + , and mixtures thereof.
  • Alkylaryl refers to organic radicals consisting of an alkyl radical and an aromatic radical. Typical examples of such radicals include, but are not limited to, alkylbenzene radicals such as benzyl, butylbenzene radicals, nonylbenzene radicals, decylbenzene radicals, undecylbenzene radicals, dodecylbenzene radicals, tridecylbenzene radicals, and the like.
  • such surfactants are selected from linear or branched alkylbenzenesulfonates of the formula A-1
  • R' and R" together contain 9 to 19, preferably 11 to 15 and in particular 11 to 13 C atoms.
  • a particularly preferred representative can be described by the formula A-1a:
  • the compound of formula (I) is preferably the sodium salt of a linear alkylbenzenesulfonate.
  • the at least one compound from the class of anionic surfactants of formula (I) is contained in the washing or cleaning agent in an amount of 0.001 to 30 wt.%, preferably 0.001 - 10 wt.%, further preferably 2 - 6 wt.%, even more preferably 3 - 5 wt.%, in each case based on the total weight of the cleaning agent.
  • the agents according to the invention preferably contain at least one anionic surfactant of the formula R 1 -O-(AO) n -SO 3 - X + (II).
  • R 1 represents a linear or branched, substituted or unsubstituted alkyl, aryl, or alkylaryl radical, preferably a linear, unsubstituted alkyl radical, particularly preferably a fatty alcohol radical.
  • Preferred R 1 radicals are selected from decyl, undecyl, dodecyl, tridecyl, tetradecyl, pentadecyl, hexadecyl, heptadecyl, octadecyl, nonadecyl, eicosyl radicals, and mixtures thereof, with preference given to those having an even number of carbon atoms.
  • R 1 radicals are derived from C 12 -C 18 fatty alcohols, for example from coconut fatty alcohol, tallow fatty alcohol, lauryl, myristyl, cetyl, or stearyl alcohol, or from C 10 -C 20 oxo alcohols.
  • AO stands for an ethylene oxide (EO) or propylene oxide (PO) group, preferably for an ethylene oxide group.
  • the index n stands for an integer from 1 to 50, preferably from 1 to 20 and in particular from 2 to 10. Most preferably, n stands for the numbers 2, 3, 4, 5, 6, 7 or 8.
  • X stands for a monovalent cation or the nth part of an n-valent cation, preferred are the alkali metal ions and among them Na + or K + , with Na + being extremely preferred. Further cations X+ can be selected from NH 4 + , 1 ⁇ 2 Zn 2+ , 1 ⁇ 2 Mg 2+ , 1 ⁇ 2 Ca 2+ , 1 ⁇ 2 Mn 2+ , and mixtures thereof.
  • the cleaning agent contains the at least one anionic surfactant of formula (II) in an amount of 2-10 wt.%, preferably 3-8 wt.%, based on the total weight of the cleaning agent.
  • anionic surfactants that can be used are the alkyl sulfates of the formula R 2 -O-SO 3 - X + (III).
  • R 2 represents a linear or branched, substituted or unsubstituted alkyl radical, preferably a linear, unsubstituted alkyl radical, particularly preferably a fatty alcohol radical.
  • Preferred R 2 radicals are selected from decyl, undecyl, dodecyl, tridecyl, tetradecyl, pentadecyl, hexadecyl, heptadecyl, octadecyl, nonadecyl, eicosyl radicals, and mixtures thereof, with preference being given to those having an even number of carbon atoms.
  • R 2 radicals are derived from C 12 -C 18 fatty alcohols, for example from coconut fatty alcohol, tallow fatty alcohol, lauryl, myristyl, cetyl, or stearyl alcohol, or from C 10 -C 20 oxo alcohols.
  • Y represents a monovalent cation or the nth part of an n-valent cation. Alkali metal ions are preferred, including Na + or K + , with Na + being most preferred.
  • Other cations Y + can be selected from NH4 + , 1 ⁇ 2 Zn2 + , 1 ⁇ 2 Mg2 + , 1 ⁇ 2 Ca2 + , 1 ⁇ 2 Mn2 + , and mixtures thereof.
  • the agent may contain at least one other surfactant in addition to the anionic surfactants described above, in particular those of formulas (I)-(III), or alternatively thereto.
  • Alternative or additional surfactants include, in particular, other anionic surfactants, nonionic surfactants, and mixtures thereof, as well as cationic, zwitterionic, and amphoteric surfactants.
  • the agents comprise at least one non-ionic surfactant, in particular at least one fatty alcohol alkoxylate.
  • R 3 represents a linear or branched, substituted or unsubstituted alkyl radical, preferably a linear, unsubstituted alkyl radical, particularly preferably a fatty alcohol radical.
  • Preferred R 2 radicals are selected from decyl, undecyl, dodecyl, tridecyl, tetradecyl, pentadecyl, hexadecyl, heptadecyl, octadecyl, nonadecyl, eicosyl radicals, and mixtures thereof, with preference given to those having an even number of carbon atoms.
  • R 3 radicals are derived from C 12 -C 18 fatty alcohols, for example from coconut fatty alcohol, tallow fatty alcohol, lauryl, myristyl, cetyl, or stearyl alcohol, or from C 10 -C 20 oxo alcohols.
  • AO represents an ethylene oxide (EO) or propylene oxide (PO) group, preferably an ethylene oxide group.
  • the index m represents an integer from 1 to 50, preferably from 1 to 20, and in particular from 2 to 10. Most preferably, m represents the numbers 2, 3, 4, 5, 6, 7, or 8.
  • nonionic surfactants which may be contained in the described agents within the meaning of the present invention include, but are not limited to, alkyl glycosides, alkoxylated fatty acid alkyl esters, amine oxides, fatty acid alkanolamides, hydroxy mixed ethers, sorbitan fatty acid esters, polyhydroxy fatty acid amides and alkoxylated alcohols.
  • Suitable amphoteric surfactants are, for example, betaines of the formula (R iii )(R iv )(R v )N + CH 2 COO - , in which R iii is an alkyl radical optionally interrupted by heteroatoms or heteroatom groups having 8 to 25, preferably 10 to 21 carbon atoms and R iv and R v are identical or different alkyl radicals having 1 to 3 carbon atoms, in particular C 10 -C 18 alkyldimethylcarboxymethylbetaine and C 11 -C 17 alkylamidopropyldimethylcarboxymethylbetaine.
  • Suitable cationic surfactants include the quaternary ammonium compounds of the formula (R vi )(R vii )(R viii )(R ix )N + X - , in which R vi to R ix represent four identical or different, in particular two long-chain and two short-chain, alkyl radicals and X - represents an anion, in particular a halide ion, for example didecyldimethylammonium chloride, alkylbenzyldidecylammonium chloride and mixtures thereof.
  • Suitable cationic surfactants are the quaternary surface-active compounds, in particular those containing a sulfonium, phosphonium, iodonium or arsonium group, which are also known as antimicrobial agents.
  • the agent can be given an antimicrobial effect or its antimicrobial effect, which may already be present due to other ingredients, can be improved.
  • the total amount of surfactants based on the weight of the agent is 2 to 30 wt.%, preferably 5 to 25 wt.%, more preferably 10 to 20 wt.%, most preferably 14 to 18 wt.%, wherein the (linear) alkylbenzenesulfonates are present at most in an amount of 0.001 to 30 wt.%, preferably 0.001 - 10 wt.%, further preferably 2 - 6 wt.%, more preferably 3 - 5 wt.%, based on the weight of the agent.
  • Detergents or cleaning agents according to the invention can contain other enzymes in addition to cutinase. These can be hydrolytic enzymes or other enzymes in a concentration appropriate for the effectiveness of the agent.
  • One embodiment of the invention thus represents agents that comprise one or more enzymes.
  • Preferred enzymes are all enzymes that can exhibit catalytic activity in the agent according to the invention, in particular a protease, amylase, cellulase, hemicellulase, mannanase, tannase, xylanase, xanthanase, xyloglucanase, ⁇ -glucosidase, pectinase, carrageenase, perhydrolase, oxidase, oxidoreductase or a lipase, as well as mixtures thereof.
  • Enzymes are advantageously present in the agent in an amount of 1 x 10 -8 to 5 wt.%, based on active protein.
  • each enzyme is present in agents according to the invention in an amount of 1 x 10 -7 -3 wt. %, from 0.00001-1 wt. %, from 0.00005-0.5 wt. %, from 0.0001 to 0.1 wt. %, and particularly preferably from 0.0001 to 0.05 wt. %, based on active protein.
  • the enzymes exhibit synergistic cleaning performance against specific soils or stains, i.e., the enzymes contained in the agent composition support one another in their cleaning performance. Synergistic effects can occur not only between different enzymes, but also between one or more enzymes and other ingredients of the agent according to the invention.
  • the amylase(s) is/are preferably an ⁇ -amylase.
  • the hemicellulase is preferably a ⁇ -glucanase, a pectinase, a pullulanase, and/or a mannanase.
  • the cellulase is preferably a cellulase mixture or a single-component cellulase, preferably or predominantly an endoglucanase and/or a cellobiohydrolase.
  • the oxidoreductase is preferably an oxidase, in particular a choline oxidase, or a perhydrolase.
  • proteases used are primarily alkaline serine proteases. They act as nonspecific endopeptidases, meaning they hydrolyze any acid amide bonds found within peptides or proteins, thereby breaking down protein-containing soils on the items being cleaned. Their pH optimum is usually in the significantly alkaline range.
  • the protein concentration can be determined using known methods, for example, the BCA method (bicinchoninic acid; 2,2'-biquinolyl-4,4'-dicarboxylic acid) or the biuret method.
  • the active protein concentration is determined by titrating the active sites using a suitable irreversible inhibitor (for proteases, for example, phenylmethylsulfonyl fluoride (PMSF)) and determining the residual activity (see also: M. Bender et al., J. Am. Chem. Soc. 88, 24 (1966), pp. 5890-5913 ).
  • a suitable irreversible inhibitor for proteases, for example, phenylmethylsulfonyl fluoride (PMSF)
  • the enzymes to be used can also be formulated together with accompanying substances, such as those from fermentation.
  • the enzymes are preferably used as liquid enzyme formulation(s).
  • the enzymes are generally not provided in the form of pure protein, but rather in the form of stabilized, storable, and transportable preparations.
  • These prefabricated preparations include, for example, solid preparations obtained by granulation, extrusion, or lyophilization or, particularly in the case of liquid or gel-like products, solutions of the enzymes, preferably as concentrated as possible, with little water content, and/or containing stabilizers or other additives.
  • the enzymes can be encapsulated for both solid and liquid dosage forms, for example by spray-drying or extrusion of the enzyme solution together with a preferably natural polymer, or in the form of capsules, for example those in which the enzymes are enclosed as if in a solidified gel, or in those of the core-shell type, in which an enzyme-containing core is coated with a water-, air-, and/or chemical-impermeable protective layer.
  • Additional active ingredients such as stabilizers, emulsifiers, pigments, bleaching agents, or dyes, can be applied in superimposed layers.
  • Such capsules are applied using methods known per se, for example by shake or roll granulation or in fluid-bed processes. Such granules, for example by applying polymeric film formers, are advantageously low in dust and, due to the coating, are stable during storage.
  • the agent according to the invention may comprise one or more enzyme stabilizers. Therefore, the agent according to the invention may further comprise an enzyme stabilizer selected, for example, from the group consisting of sodium formate, sodium sulfate, lower aliphatic alcohols, and boric acid, as well as their esters and salts. Of course, two or more of these compounds may also be used in combination.
  • the salts of the compounds mentioned can also be used in the form of hydrates, such as sodium sulfate decahydrate.
  • lower aliphatic alcohols includes monoalcohols, diols, and higher-hydric alcohols with up to 6 carbon atoms.
  • Polyols belonging to the group of lower aliphatic alcohols include, in particular, polyols, for example, glycerol, (mono)ethylene glycol, (mono)propylene glycol, or sorbitol, without the invention being limited to these.
  • composition according to the invention may also contain at least one further stabilizer.
  • Such stabilizers are known in the art.
  • Reversible protease inhibitors protect the enzymes contained in a detergent or cleaning agent from proteolytic degradation by reversibly inhibiting the enzymatic activity of the proteases contained in the agent.
  • Benzamidine hydrochloride, boronic acids, or their salts or esters are frequently used as reversible protease inhibitors, especially derivatives with aromatic groups, such as ortho-, meta-, or para-substituted phenylboronic acids, especially 4-formylphenyl boronic acid, or the salts or esters of the aforementioned compounds.
  • Peptide aldehydes i.e., oligopeptides with a reduced C-terminus, especially those consisting of 2 to 50 monomers, are also used for this purpose.
  • Peptide reversible protease inhibitors include, among others, ovomucoid and leupeptin.
  • enzyme stabilizers include amino alcohols such as mono-, di-, triethanolamine, and propanolamine and their mixtures; aliphatic carboxylic acids up to C12 , such as succinic acid; other dicarboxylic acids; or salts of these acids. End-capped fatty acid amide alkoxylates are also suitable for this purpose. Some organic acids used as builders can also stabilize an enzyme. Calcium and/or magnesium salts, such as calcium acetate, are also used for this purpose.
  • Polyamide oligomers or polymeric compounds such as lignin, water-soluble vinyl copolymers or cellulose ethers, acrylic polymers, and/or polyamides stabilize the enzyme preparation against, among other things, physical influences or pH fluctuations.
  • Polyamine N-oxide-containing polymers act simultaneously as enzyme stabilizers and dye transfer inhibitors.
  • Other polymeric stabilizers are linear C 8 -C 18 polyoxyalkylenes.
  • Alkyl polyglycosides can also stabilize the enzymatic components of the agent according to the invention and, preferably, can additionally enhance their performance.
  • Crosslinked Nitrogen-containing compounds preferably fulfill a dual function as soil-release agents and enzyme stabilizers. Hydrophobic, non-ionic polymers particularly stabilize any cellulase present.
  • Reducing agents and antioxidants increase the stability of enzymes against oxidative degradation; for example, sulfur-containing reducing agents, such as sodium sulfite and reducing sugars, are common for this purpose.
  • the washing or cleaning agents according to the present invention are liquid and contain water as the main solvent, i.e., they are aqueous agents.
  • the water content of the aqueous agent according to the invention is typically 15 to 70 wt.%, preferably 20 to 60 wt.%. In various embodiments, the water content is more than 5 wt.%, preferably more than 15 wt.%, and particularly preferably more than 50 wt.%, in each case based on the total amount of agent.
  • Non-aqueous solvents may also be added to the product.
  • Suitable non-aqueous solvents include mono- or polyhydric alcohols, alkanolamines, or glycol ethers, provided they are miscible with water within the specified concentration range.
  • the solvents are preferably selected from ethanol, n-propanol, i-propanol, butanols, glycol, propanediol, butanediol, methylpropanediol, glycerin, diglycol, propyl diglycol, butyl diglycol, hexylene glycol, ethylene glycol methyl ether, ethylene glycol ethyl ether, ethylene glycol propyl ether, ethylene glycol mono-n-butyl ether, diethylene glycol methyl ether, diethylene glycol ethyl ether, propylene glycol methyl ether, propylene glycol ethyl ether, propylene glycol propy
  • the one or more non-aqueous solvents are usually present in an amount of 0.1 to 10 wt.%, preferably 1 to 8 wt.%, based on the total composition.
  • the agents according to the invention may contain other ingredients that further improve the application and/or aesthetic properties of the cleaning agent.
  • these include, for example, additives to improve drainage and drying properties, to adjust viscosity and/or for stabilization, as well as other auxiliaries and additives commonly found in cleaning agents, such as UV stabilizers, perfume, pearlescent agents, dyes, corrosion inhibitors, preservatives, bittering agents, organic Salts, disinfectants, structuring polymers, defoamers, encapsulated ingredients (e.g. encapsulated perfume), pH adjusters and skin-feel-improving or caring additives.
  • additives to improve drainage and drying properties to adjust viscosity and/or for stabilization
  • auxiliaries and additives commonly found in cleaning agents such as UV stabilizers, perfume, pearlescent agents, dyes, corrosion inhibitors, preservatives, bittering agents, organic Salts, disinfectants, structuring polymers, defoamers, encapsulated ingredients (e.g. encapsulated perfume), pH adjusters and skin-feel
  • a washing or cleaning agent according to the invention preferably contains at least one water-soluble and/or water-insoluble, organic and/or inorganic builder.
  • Generally applicable builders include, in particular, aminocarboxylic acids and their salts, zeolites, silicates, carbonates, organic (co)builders, and—where there are no environmental objections to their use—phosphates.
  • these agents are preferably phosphate-free.
  • Water-soluble organic builder substances include polycarboxylic acids, especially citric acid and sugar acids, monomeric and polymeric aminopolycarboxylic acids, especially methylglycinediacetic acid, nitrilotriacetic acid, and ethylenediaminetetraacetic acid, as well as polyaspartic acid, polyphosphonic acids, especially aminotris(methylenephosphonic acid), ethylenediaminetetrakis(methylenephosphonic acid), and 1-hydroxyethane-1,1-diphosphonic acid, polymeric hydroxy compounds such as dextrin, as well as polymeric (poly)carboxylic acids, polymeric acrylic acids, methacrylic acids, maleic acids, and copolymers thereof, which may also contain small amounts of polymerizable substances without carboxylic acid functionality.
  • polycarboxylic acids especially citric acid and sugar acids
  • monomeric and polymeric aminopolycarboxylic acids especially methylglycinediacetic acid, nitrilotriacetic acid, and
  • Suitable, albeit less preferred, compounds of this class are copolymers of acrylic acid or methacrylic acid with vinyl ethers, such as vinyl methyl ethers, vinyl esters, ethylene, propylene, and styrene, in which the proportion of acid is at least 50% by weight.
  • the organic builders can be used, particularly for the production of liquid agents, in the form of aqueous solutions, preferably in the form of 30 to 50 percent by weight aqueous solutions. All of the acids mentioned are generally used in the form of their water-soluble salts, especially their alkali metal salts.
  • Organic builders can, if desired, be present in amounts of up to 40 wt.%, in particular up to 25 wt.%, and preferably from 1 wt.% to 8 wt.%. Amounts close to the stated upper limit are preferably used in paste-like or liquid, especially water-based, compositions according to the invention. Laundry aftertreatment compositions according to the invention, such as fabric softeners, can optionally also be free of organic builders.
  • Alkali silicates and, if there are no concerns about their use, polyphosphates, preferably sodium triphosphate, are particularly suitable as water-soluble inorganic builder materials.
  • Builder materials can be used, in particular crystalline or amorphous alkali aluminosilicates, if desired, in amounts of up to 50 wt.%, preferably not more than 40 wt.%, and in liquid compositions, in particular from 1 wt.% to 5 wt.%.
  • crystalline sodium aluminosilicates of detergent quality in particular zeolite A, P, and optionally X, are preferred. Amounts close to the stated upper limit are preferably used in solid, particulate compositions.
  • Suitable aluminosilicates in particular, have no particles with a grain size exceeding 30 ⁇ m and preferably consist of at least 80 wt.% particles with a size below 10 ⁇ m.
  • Suitable substitutes or partial substitutes for the aluminosilicate mentioned are crystalline alkali silicates, which can be present alone or in a mixture with amorphous silicates.
  • the alkali silicates usable as builders in the agents according to the invention preferably have a molar ratio of alkali oxide to SiO 2 of less than 0.95, in particular from 1:1.1 to 1:12, and can be amorphous or crystalline.
  • Preferred alkali silicates are sodium silicates, in particular amorphous sodium silicates, with a molar Na 2 O:SiO 2 ratio of 1:2 to 1:2.8.
  • crystalline silicates which can be present alone or in a mixture with amorphous silicates
  • Preferred crystalline layered silicates are those in which x in the general formula mentioned assumes the values 2 or 3.
  • beta- and delta-sodium disilicates Na 2 Si 2 O 5 y H 2 O
  • the weight ratio of aluminosilicate to silicate is preferably 1:10 to 10:1.
  • the weight ratio of amorphous alkali silicate to crystalline alkali silicate is preferably 1:2 to 2:1 and in particular 1:1 to 2:1.
  • Builder substances are, if desired, present in the compositions according to the invention preferably in amounts of up to 60% by weight, in particular from 5% to 40% by weight.
  • Water-soluble builders are particularly preferred in liquid formulations.
  • Laundry aftertreatment compositions according to the invention, such as fabric softeners, are preferably free of inorganic builders.
  • Polymeric thickeners within the meaning of the present invention are the polycarboxylates which act as polyelectrolytes to thicken, preferably homo- and copolymers of acrylic acid, in particular acrylic acid copolymers such as acrylic acid-methacrylic acid copolymers, and the polysaccharides, in particular heteropolysaccharides, as well as other customary thickening polymers.
  • Suitable polysaccharides or heteropolysaccharides are polysaccharide gums, for example, gum arabic, agar, alginates, carrageenans and their salts, guar, guaran, tragacanth, gellan gum, ramsan, dextran, or xanthan gum and their derivatives, e.g., propoxylated guar gum, as well as their mixtures.
  • Other polysaccharide thickeners such as starches or cellulose derivatives, can be used alternatively, but preferably in addition, to a polysaccharide gum.
  • starches of various origins include starches of various origins and starch derivatives, e.g., hydroxyethyl starch, starch phosphate esters, or starch acetates, or carboxymethylcellulose or its sodium salt, methyl, ethyl, hydroxyethyl, hydroxypropyl, hydroxypropylmethyl, or hydroxyethylmethylcellulose, or cellulose acetate.
  • starch derivatives e.g., hydroxyethyl starch, starch phosphate esters, or starch acetates, or carboxymethylcellulose or its sodium salt, methyl, ethyl, hydroxyethyl, hydroxypropyl, hydroxypropylmethyl, or hydroxyethylmethylcellulose, or cellulose acetate.
  • Acrylic acid polymers suitable as polymeric thickeners are, for example, high molecular weight homopolymers of acrylic acid (INCI Carbomer) crosslinked with a polyalkenyl polyether, in particular an allyl ether of sucrose, pentaerythritol or propylene, which are also referred to as carboxyvinyl polymers.
  • a polyalkenyl polyether in particular an allyl ether of sucrose, pentaerythritol or propylene, which are also referred to as carboxyvinyl polymers.
  • Particularly suitable polymeric thickeners are the following acrylic acid copolymers: (i) Copolymers of two or more monomers from the group of acrylic acid, methacrylic acid and their simple esters, preferably formed with C 1-4 alkanols (INCI Acrylates Copolymer), which include the copolymers of methacrylic acid, butyl acrylate and methyl methacrylate ( CAS 25035-69-2 ) or butyl acrylate and methyl methacrylate ( CAS 25852-37-3 ); (ii) cross-linked high molecular weight acrylic acid copolymers, which include, for example, copolymers of C 10-30 alkyl acrylates cross-linked with an allyl ether of sucrose or pentaerythritol with one or more monomers from the group consisting of acrylic acid, methacrylic acid and their simple esters, preferably formed with C 1-4 alkanols (INCI Acrylates/C10-30 Alkyl Acrylate Crosspolymer).
  • the content of polymeric thickener is usually not more than 8 wt.%, preferably between 0.1 and 7 wt.%, particularly preferably between 0.5 and 6 wt.%, in particular between 1 and 5 wt.% and most preferably between 1.5 and 4 wt.%, for example between 2 and 2.5 wt.%, based on the total weight of the agent.
  • one or more dicarboxylic acids and/or their salts can be added, in particular a composition of Na salts of adipic, succinic and glutaric acid, as described, for example, in the It is available under the trade name Sokalan® DSC. It is advantageously used in amounts of 0.1 to 8 wt.%, preferably 0.5 to 7 wt.%, in particular 1.3 to 6 wt.%, and particularly preferably 2 to 4 wt.%, based on the total weight of the cleaning agent. However, if their use can be omitted, the agent according to the invention is preferably free of dicarboxylic acid (salts).
  • the detergents according to the invention can be compared with reference detergents to determine the increased anti-pilling performance of the detergents according to the invention.
  • a detergent system can be composed as follows (all data in percent by weight): Reference detergent: 4.4% alkylbenzenesulfonic acid, 5.6% other anionic surfactants, 2.4% C12-C18 Na salts of fatty acids (soaps), 4.4% nonionic surfactants, 0.2% phosphonates, 1.4% citric acid, 0.95% NaOH, 0.01% defoamers, 2.0% glycerin, 0.08% preservatives, 1% ethanol, 1.6% enzyme mix (protease, amylase, cellulase, mannase), the remainder demineralized water.
  • Inventive agent 4.4% alkylbenzenesulfonic acid, 5.6% other anionic surfactants, 2.4% C12-C18 sodium salts of fatty acids (soaps), 4.4% nonionic surfactants, 0.2% phosphonates, 1.4% citric acid, 0.95% NaOH, 0.01% defoamer, 2.0% glycerin, 0.08% preservatives, 1% ethanol, 1.6% enzyme mix (protease, amylase, cellulase, mannase), 0.009% cutinase, balance demineralized water.
  • the preferred dosage of the liquid detergent is between 4.5 and 6.0 grams per liter of wash liquor, for example, 4.7, 4.9, or 5.9 grams per liter of wash liquor. Washing is preferably carried out in a pH range between pH 8 and pH 10.5, preferably between pH 8 and pH 9.
  • the aforementioned embodiments of the present invention encompass all solid, powdered, liquid, gel-like, or pasty dosage forms of washing or cleaning agents according to the invention, which may optionally also consist of multiple phases and may be present in compressed or uncompressed form.
  • the agent may be in the form of a free-flowing powder, in particular with a bulk density of 300 g/l to 1200 g/l, in particular 500 g/l to 900 g/l or 600 g/l to 850 g/l.
  • Solid dosage forms of the agent also include extrudates, granules, tablets, or pouches.
  • the agent may also be liquid, gel-like, or pasty, for example in the form of a non-aqueous liquid detergent or a non-aqueous paste, or in the form of an aqueous liquid detergent or a water-containing paste.
  • the agent may be in the form of a one-component system. Such agents consist of one phase.
  • a product can also consist of multiple phases, for example, single-phase, double-phase, or multi-phase. Such a product is therefore divided into several components (multi-component system).
  • the invention further relates to a process for cleaning textiles, characterized in that an agent according to the invention is used in at least one process step.
  • the textiles preferably contain or consist of polyester.
  • the process described above is characterized in that the agent according to the invention is used at a temperature of 0-100°C, preferably 0-80°C, more preferably 30-70°C, and most preferably 40-60°C.
  • Processes for cleaning textiles are generally characterized by the fact that various cleaning-active substances are applied to the item to be cleaned in several process steps and are washed off after the contact time, or that the item to be cleaned is treated in some other way with a detergent or a solution or dilution of this agent. All conceivable washing or cleaning processes can be enriched in at least one of the process steps by the use of a washing or cleaning agent according to the invention and then represent embodiments of the present invention. All facts, objects and embodiments described for agents according to the invention are also applicable to this subject matter of the invention. Therefore, express reference is made here to the disclosure at the appropriate point with the note that this disclosure also applies to the above inventive processes.
  • a single and/or the only step of such a process can consist of bringing a cutinase, the sole cleaning-active component, into contact with the soil, preferably in a buffer solution or water.
  • Alternative embodiments of this subject matter of the invention also include processes for treating textile raw materials or for textile care, in which an agent according to the invention is active in at least one process step.
  • processes for textile raw materials, fibers, or textiles with synthetic components are preferred, and especially for those with polyester.
  • the invention also covers the use of the washing or cleaning agent described herein as described above for the (improved) removal of soiling, for example from textiles, in particular polyester textiles.
  • the invention also relates to the use of a cutinase for reducing pilling effects of a detergent, particularly preferably a liquid detergent, wherein the detergent contains the cutinase.
  • the cutinase is a cutinase as defined herein.
  • the cutinase is present in the detergent in an amount of 0.00001-1 wt.%, preferably in an amount of 0.0001-0.5 wt.%, particularly preferably in an amount of 0.001-0.1 wt.%.
  • the cutinase, which reduces the pilling effect is applied to textiles, in particular textiles made of polyester or comprising polyester.
  • the gene was synthetically produced with codon optimization for expression in E. coli (SEQ ID NO: 3) and then cloned into the NdeI and XhoI sites of the PET-21b vector from Novagen, creating a C-terminal fusion with a 6x histidine tag.
  • the plasmid confirmed by sequencing, is transformed into electrocompetent E. coli BL21(DE3)CodonPlus RIPL cells (Agilent Technologies) using standard transformation protocols.
  • the cells are harvested by centrifugation and resuspended in lysis buffer (50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 20 mM imidazole). Cell disruption is performed using ultrasonication, followed by centrifugation (15,000 x g, 20 min, 4°C). The supernatant contains the His-tagged LC cutinase.
  • lysis buffer 50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 20 mM imidazole.
  • Cell disruption is performed using ultrasonication, followed by centrifugation (15,000 x g, 20 min, 4°C). The supernatant contains the His-tagged LC cutinase.
  • NiNTA Purification using NiNTA (Qiagen, Hilden, DE) is carried out according to the manufacturer Qiagen’s protocol.
  • the degradation kinetics of the polyesterases described here were analyzed using QCM-D (quartz crystal microbalance with dissipation monitoring).
  • the polyester model substrates were prepared starting from commercially available AT-cut QCM-D crystals (Biolin Scientific) with a typical resonance frequency of 5 MHz and a gold-terminated electrode. These crystals were modified with a polyester thin film using a spin-coating process.
  • Substrate PET film: 0.25 mm thick, amorphous, transparent from GoodFellow Cambridge Limited, ES301445 Carry out in 2 mL tubes
  • PET film (0.5 cm x 2.5 cm) was washed with (1) 1.7 mL of 0.1% SDS (w/v), 50 °C, 30 min, 400 rpm; (2) 1.5 mL of absolute ethanol, 50 °C, 5 min, 700 rpm; (3) 1.7 mL of double-distilled water, 50 °C, 5 min, 700 rpm, and dried to constant value for 48 h at 50 °C and weighed. This was followed by incubation (48–72 h at 55 °C) with a fixed amount of cutinase according to SEQ ID NO:1 (5, 25, 50 ⁇ g) in a total volume of 1.7 mL in a detergent matrix (see below, dosage 4.7 g/L).
  • the PET film was washed with (1) 1.7 mL of 0.1% SDS (w/v) + 0.5% Triton X-100 (w/v), 50 °C, 30 min, 400 rpm; (2) 1.5 mL of absolute ethanol, 50 °C, 5 min, 700 rpm; (3) 1.7 mL of double-distilled water, 50 °C, 5 min, 700 rpm; and dried to constant temperature for 48 h at 50 °C and weighed. The weight loss was determined.
  • a concentration-dependent PET degradation caused by the cutinase in the detergent matrix is clearly visible.
  • the pre-pilled fabrics are produced by 20 repeated washing cycles at 40°C in standard washing machines.
  • the cutinase according to the invention significantly improves the pill appearance.

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Claims (10)

  1. Détergent ou agent nettoyant, caractérisé en ce qu'il contient une cutinase qui présente au moins 65 % d'identité de séquence avec la séquence d'acides aminés spécifiée dans SEQ ID NO:1 sur toute sa longueur.
  2. Agent selon la revendication 1, caractérisé en ce que la cutinase comprend une séquence d'acides aminés qui est identique à la séquence d'acides aminés spécifiée dans SEQ ID NO:1 sur toute sa longueur au moins dans une mesure de 66 %, 67 %, 68 %, 69 %, 70 %, 71 %, 72 %, 73 %, 74 %, 75 %, 76 %, 77 %, 78 %, 79 %, 80 %, 81 %, 82 %, 83 %, 84 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 90,5 %, 91 %, 91,5 %, 92 %, 92,5 %, 93 %, 93,5 %, 94 %, 94,5 %, 95 %, 95,5 %, 96 %, 96,5 %, 97 %, 97,5 %, 98 %, 98,5 %, 98,8 %, 99,0 %, 99,2 %, 99,4 % ou 99,6 %.
  3. L'agent selon la revendication 1 ou 2, caractérisé en ce que
    (1) la cutinase peut être obtenue à partir d'une cutinase selon la revendication 1 ou 2 comme molécule de départ par une ou plusieurs substitutions conservatrices d'acides aminés ; et/ou
    (2) la cutinase peut être obtenue à partir d'une cutinase selon la revendication 1 ou 2 comme molécule de départ par fragmentation, délétion, insertion ou mutagenèse par substitution et comprend une séquence d'acides aminés qui correspond à la molécule de départ sur une longueur d'au moins 180, 190, 200, 210, 220, 230, 240, 245, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263 ou 264 acides aminés interconnectés.
  4. L'agent selon l'une quelconque des revendications 1 à 3, dans lequel la cutinase est contenue en une quantité de 0,00001 à 1 % en poids, de préférence en une quantité de 0,0001 à 0,5 % en poids, de manière particulièrement préférée en une quantité de 0,001 à 0,1 % en poids.
  5. Agent selon l'une quelconque des revendications 1 à 4, caractérisé en ce que
    a. il contient au moins un ingrédient supplémentaire choisi dans le groupe constitué par les tensioactifs, les adjuvants (matériaux structurels), les agents de blanchiment, les activateurs de blanchiment, les solvants organiques miscibles à l'eau, d'autres enzymes, les agents séquestrants, les électrolytes, les régulateurs de pH, les azurants optiques, les inhibiteurs de grisaillement, les régulateurs de mousse, les colorants et les substances aromatiques, et leurs combinaisons ; et/ou
    b. il se présente sous forme solide ou liquide, de préférence liquide.
  6. Procédé de nettoyage de textiles, caractérisé en ce qu'un agent selon l'une quelconque des revendications 1 à 5 est utilisé dans au moins une étape du procédé.
  7. Procédé selon la revendication 6, caractérisé en ce que les textiles contiennent du polyester ou sont constitués de polyester.
  8. Utilisation d'un agent selon l'une quelconque des revendications 1 à 5 pour éliminer les salissures, en particulier sur des textiles contenant du polyester.
  9. Utilisation d'une cutinase qui présente au moins 65 % d'identité de séquence avec la séquence d'acides aminés spécifiée dans SEQ ID NO:1 sur toute sa longueur pour réduire les effets de boulochage et/ou augmenter l'effet anti-grisaillement d'un agent, de préférence un détergent, en particulier un détergent liquide, dans lequel l'agent contient la cutinase.
  10. Utilisation selon la revendication 9, dans laquelle
    a. la cutinase est telle que définie dans la revendication 2 ou 3 ; et/ou
    b. la cutinase est contenue dans l'agent en une quantité telle que définie dans la revendication 4 ; et/ou
    c. l'agent est tel que défini dans la revendication 5.
EP18758569.0A 2017-08-24 2018-08-16 Propriétés d'entretien améliorées de textiles polyester ii Active EP3673035B1 (fr)

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DE102018210609A1 (de) * 2018-06-28 2020-01-02 Henkel Ag & Co. Kgaa Polyesterase II
EP4424823B1 (fr) * 2018-07-27 2025-12-03 Carbios Nouvelles estérases et leurs utilisations
EP3699272A1 (fr) * 2019-02-21 2020-08-26 Henkel AG & Co. KGaA Propriétés de soin améliorées sur des textiles en polyester par l'utilisation d'un cutinase
WO2023019222A1 (fr) * 2021-08-11 2023-02-16 Biometis Technology, Inc. Dégradation enzymatique de polyéthylène téréphtalate
WO2023116569A1 (fr) 2021-12-21 2023-06-29 Novozymes A/S Composition comprenant une lipase et un renforçateur
EP4630529A1 (fr) 2022-12-05 2025-10-15 Novozymes A/S Composition comprenant une lipase et un peptide

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AU710006B2 (en) 1995-04-28 1999-09-09 Genencor International, Inc. Detergents comprising cellulases
ES2554983T3 (es) * 2007-04-03 2015-12-28 Henkel Ag & Co. Kgaa Agente de lavado inhibidor del agrisado
DE102008017103A1 (de) 2008-04-02 2009-10-08 Henkel Ag & Co. Kgaa Wasch- und Reinigungsmittel enthaltend Proteasen aus Xanthomonas
EP2516610A1 (fr) * 2009-12-21 2012-10-31 Danisco US Inc. Compositions détergentes contenant une lipase issue de thermobifida fusca et leurs procédés d'utilisation
AR087745A1 (es) * 2011-08-31 2014-04-16 Danisco Us Inc Composiciones y metodos que comprenden una variante de enzima lipolitica
CN104024407A (zh) * 2011-12-22 2014-09-03 丹尼斯科美国公司 包含脂解酶变体的组合物和方法
DE102014204374A1 (de) * 2014-03-11 2015-09-17 Henkel Ag & Co. Kgaa PET-Esterasen und deren Verwendung
DE102016208408A1 (de) * 2016-05-17 2017-11-23 Henkel Ag & Co. Kgaa Wasch- oder Reinigungsmittel enthaltend eine Polyethylenterephthalat Hydrolase
KR102649944B1 (ko) * 2016-07-12 2024-03-22 까르비오 신규한 에스테라아제 및 그의 용도
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