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EP3673035A1 - Propriétés d'entretien améliorées de textiles polyester ii - Google Patents

Propriétés d'entretien améliorées de textiles polyester ii

Info

Publication number
EP3673035A1
EP3673035A1 EP18758569.0A EP18758569A EP3673035A1 EP 3673035 A1 EP3673035 A1 EP 3673035A1 EP 18758569 A EP18758569 A EP 18758569A EP 3673035 A1 EP3673035 A1 EP 3673035A1
Authority
EP
European Patent Office
Prior art keywords
cutinase
agent
amino acid
textiles
weight
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP18758569.0A
Other languages
German (de)
English (en)
Other versions
EP3673035B1 (fr
Inventor
Nina Mussmann
Susanne Wieland
Daniela HERBST
Margret VAN LIER
Ines Baranski
Alexander Schulz
Boray TORUN
Christina SCHÖNDELING
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Henkel AG and Co KGaA
Original Assignee
Henkel AG and Co KGaA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Henkel AG and Co KGaA filed Critical Henkel AG and Co KGaA
Publication of EP3673035A1 publication Critical patent/EP3673035A1/fr
Application granted granted Critical
Publication of EP3673035B1 publication Critical patent/EP3673035B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
    • C11D2111/10—Objects to be cleaned
    • C11D2111/12—Soft surfaces, e.g. textile

Definitions

  • the present invention is in the field of enzyme technology, in particular the anti-pilling action of enzymes, as used, for example, in detergents or cleaners.
  • the invention relates to an agent, in particular washing or cleaning agent, which contains a cutinase as defined herein.
  • the present invention relates to a method for the cleaning of textiles and the use of the agent according to the invention for the removal of stains.
  • the invention is directed to the use of a cutinase for reducing pilling effects and graying in an agent, preferably a detergent or cleaning agent.
  • Pilling refers to the formation of nodules or lint in fabrics. These small lint arise especially with short-fiber substances. Long-fiber and twisted fibers, on the other hand, produce less pilling. Generally, these nodules are caused by loose fibers in the fabric or those that have come loose from the fabric. Due to their smooth surface, synthetic fibers tend to pilling rather than natural fibers because synthetic fibers can be released from the fabric faster than rough natural fibers. In woolen fabrics, these fibers "matt” mainly by mechanical friction and form nodules on the surface.
  • Pilling takes place especially on mechanically stressed areas, usually these are shoulder and waistband area. Due to the continuous thinning of the material, these stressed areas are in particular at risk of forming or even breaking holes. The unwanted pilling has the consequence that accordingly impaired textiles of the
  • Polyester textiles there is no comparable way to reduce pilling. Therefore, it is desirable and there is a demand for solutions that reduce the pilling of textiles, especially textiles containing synthetic fibers such as polyester, to make clothes look as new as possible for as long as possible.
  • the colors should remain strong, the shape should be preserved and the surfaces should remain smooth and undamaged.
  • Cutinase identified in a foliage compost by a metagenomic approach is active under washing process conditions and has various care properties for PET textiles. This is surprising to the extent that previously known cutinases and PET esterases tend to be higher
  • the present invention is directed to an agent, in particular a detergent or cleaning agent, characterized in that it contains a cutinase having at least 65% sequence identity with the amino acid sequence given in SEQ ID NO: 1 over its entire length.
  • the present invention is directed to processes for the purification of textiles, characterized in that in at least one process step inventive agent is applied.
  • the textiles are preferably polyester-containing textiles or consist of polyester.
  • the present invention is further directed to the use of an agent as described herein, preferably a detergent or cleaning agent, more preferably a liquid detergent, to remove soils.
  • an agent as described herein preferably a detergent or cleaning agent, more preferably a liquid detergent, to remove soils.
  • another aspect of the invention involves the use of the cutinase described herein to reduce pilling effects and / or increase the anti-gray activity of an agent, preferably a detergent or cleaning agent, more preferably a liquid detergent, the agent containing the Contains cutinase.
  • an agent preferably a detergent or cleaning agent, more preferably a liquid detergent, the agent containing the Contains cutinase.
  • a cutinase (EC 3.1.1.74) or cutin hydrolase is an enzyme that belongs to the a / ß-hydrolases and hydrolyzes cutin.
  • cutinase is formed by some phytopathogenic fungi and bacteria (through the cutinase the fungi are able to break down the ester bond of the cutin in the cuticle of the plants and thus penetrate into the plants).
  • the cutinase is a cutinase having at least 70% sequence identity with the amino acid sequence given in SEQ ID NO: 1 over its entire length.
  • the cutinase present in the agent according to the invention comprises that indicated in SEQ ID NO: 1
  • the invention also includes cutinases derived from the
  • the invention also includes cutinases obtainable by expression of the nucleotide sequence of SEQ ID NO: 3. In one aspect of the invention, the invention also encompasses the nucleotide sequence according to SEQ ID NO: 3 and
  • the cutinase comprises a
  • the means is characterized in that
  • the cutinase is obtainable from a cutinase as defined above as a parent molecule by single or multiple conservative amino acid substitution; and or
  • the cutinase is obtainable from a cutinase as defined above as the parent molecule by fragmentation, deletion, insertion or substitution mutagenesis and a
  • the agents according to the invention preferably contain the cutinase in an amount of 0.00001-1% by weight, more preferably in an amount of 0.0001-0.5% by weight, particularly preferably in an amount of 0.001-0.1 Wt .-%, each based on the active protein.
  • sequence comparison is based on the BLAST algorithm established and commonly used in the prior art (see, for example, Altschul, SF, Gish, W., Miller, W., Myers, EW & Lipman, DJ. (1990) "Basic local alignment search Biol. 215: 403-410; and Altschul, Stephan F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Hheng Zhang, Webb Miller, and David J.
  • Lipman (1997): "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs"; Nucleic Acids Res., 25, pp.3389-3402) and is in principle accomplished by similar sequences of nucleotides or amino acids in the nucleic acid or amino acid sequences of each other be assigned. A tabular assignment of the respective positions is referred to as alignment.
  • Another algorithm available in the prior art is the FASTA algorithm. Sequence comparisons (alignments), in particular multiple sequence comparisons, are created with computer programs.
  • T-Coffee see, for example, Notredame et al (2000): T-Coffee: A novel method for multiple sequence alignments, J. Mol. Biol. 302, 205-217 or programs based on these programs or algorithms.
  • alignment comparisons with the computer program Vector NTI® Suite 10.3 (Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, California, USA) with the default parameters whose AlignX module for sequence comparisons is based on ClustalW.
  • Identity and / or homology information can be made about whole polypeptides or genes or only over individual regions. Homologous or identical regions of different nucleic acid or amino acid sequences are therefore defined by matches in the sequences. Such areas often have identical functions. They can be small and comprise only a few nucleotides or amino acids. Often, such small regions exert essential functions for the overall activity of the protein. It may therefore be useful to relate sequence matches only to individual, possibly small areas. Unless otherwise indicated, identity or homology information in the present application, however, refers to the total length of the particular nucleic acid or amino acid sequence indicated.
  • the cutinase comprises an amino acid sequence corresponding to the amino acid sequence given in SEQ ID NO: 1 over its total length of at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88 %, 89%, 90%, 90.5%, 91%, 91, 5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95, 5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5%, 98.8%, 99.0%, 99.2%, 99.4% or 99.6 % is homologous.
  • the cutinase is characterized in that its anti-pilling performance is not significantly reduced compared to that of a cutinase which comprises an amino acid sequence corresponding to the amino acid sequences given in SEQ ID NO: 1, ie at least 70%. , 75%, 80%, 85%, 90%, 95% of the reference anti-pilling benefits.
  • the anti-pilling performance can be determined in a washing system containing a detergent in a dosage between 4.5 and 7.0 grams per liter of wash liquor and the cutinase, wherein the cutinases to be compared are used in concentration (based on active protein) and the anti-pilling performance is determined as described herein.
  • the washing process for 60 minutes at a temperature of 60 ° C and the water have a water hardness between 15.5 and 16.5 ° (German hardness).
  • the concentration of cutinase in the washing agent intended for this washing system is from 0.00001 to 1% by weight, preferably from 0.0001 to 0.5% by weight, particularly preferably from 0.001 to 0.1% by weight, based on active, purified protein.
  • a preferred liquid detergent for such a washing system is composed as follows (all figures in weight percent): 4.4% alkyl benzene sulfonic acid, 5.6% anionic surfactants, 2.4% C12-C18 Na salts of fatty acids, 4.4 % non-ionic surfactants, 0.2% phosphonates, 1, 4% citric acid, 0.95% NaOH, 0.01% antifoam, 2% glycerol, 0.08% preservatives, 1% ethanol, 1, 6% enzyme mix ( Protease, amylase, cellulase, mannase), remainder demineralised water.
  • the dosage of the liquid detergent is between 4.5 and 6.0 grams per liter of wash liquor, for example, 4.7, 4.9 or 5.9 grams per liter of wash liquor. Preference is given to washing in a pH range between pH 8 and pH 10.5, preferably between pH 8 and pH 9.
  • the anti-pilling performance can be tracked by visual matching.
  • a panel of inspectors assigns a scale of 1-5 to the laundry to be examined.
  • the activity-equivalent use of the respective cutinase ensures that, even if the ratio of active substance to total protein (the values of the specific activity) diverge, the respective enzymatic properties, for example the anti-pilling performance, are compared. In general, a low specific activity can be compensated by adding a larger amount of protein.
  • Proteins can be grouped into groups of immunologically related proteins by reaction with an antiserum or antibody.
  • the members of such a group are characterized by having the same antigenic determinant recognized by an antibody. They are therefore structurally so similar to each other that they are recognized by an antiserum or specific antibodies. Another one
  • the subject of the invention is therefore formed by cutinases which are characterized by having at least one, and more preferably two, three or four, identical antigenic determinants with a cutinase used in an agent according to the invention. Due to their immunological similarity, such cutinases are structurally so similar to the cutinases used in the agents according to the invention that a similar function is to be assumed as well. Further cutinases used in the agents according to the invention can, in comparison to the cutinase described in SEQ ID NO: 1, further amino acid changes, in particular
  • cutinases are, for example, by targeted genetic modification, i. by mutagenesis, further developed and optimized for specific applications or specific properties (for example, in terms of catalytic activity, stability, etc.). Furthermore, nucleic acids encoding the cutinases used can be introduced into recombination approaches and thus used to generate completely novel cutinases or other polypeptides.
  • the goal is to introduce into the known molecules targeted mutations such as substitutions, insertions or deletions, for example, to improve the cleaning performance of enzymes of the invention.
  • targeted mutations such as substitutions, insertions or deletions, for example, to improve the cleaning performance of enzymes of the invention.
  • the surface charges and / or the isoelectric point of the molecules and thereby their interactions with the substrate can be changed.
  • the net charge of the enzymes can be changed in order to influence the substrate binding, in particular for use in detergents and cleaners.
  • the stability of the cutinase can be further increased by one or more corresponding mutations, thereby improving its cleaning performance.
  • Another object of the invention is therefore an agent containing a cutinase, which is characterized in that it is obtainable from a cutinase as described above as the starting molecule by one or more conservative amino acid substitution.
  • the cutinase is characterized in that it is obtainable from a cutinase contained in an agent according to the invention as a starting molecule
  • Amino acid sequence over a length of at least 180, 190, 200, 210, 220, 230, 240, 245, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263 or 264 are contiguous amino acids consistent with the parent molecule.
  • hydrolytic activity i. their hydrolytic activity is at least equal to that of the starting enzyme, i. in a preferred embodiment, the hydrolytic activity is at least 80, preferably at least 90% of the activity of the
  • the cutinase may have one or more additional amino acids N- or C-terminal in addition to the sequence shown in SEQ ID NO: 1.
  • such N-terminal peptides may be the naturally occurring signal peptides for the cutinase or even a single methionine residue.
  • the cutinase has, for example, the amino acid sequence given in SEQ ID NO: 2. All embodiments disclosed above in the context of the mature cutinase of SEQ ID NO: 1 are also applicable to the cutinase having the sequence of SEQ ID NO: 2.
  • An object of the invention is an agent characterized by containing a cutinase as defined herein.
  • the agent is preferably a washing or cleaning agent.
  • compositions / agents are made, unless explicitly stated otherwise to wt .-%, each based on the respective mixture / the respective means.
  • fatty acids or fatty alcohols or their derivatives - unless otherwise stated - representative of branched or unbranched carboxylic acids or alcohols or their derivatives having preferably 6 to 22 carbon atoms.
  • the oxo alcohols or their derivatives which are obtainable, for example, by the RoELEN's oxo synthesis, can also be used correspondingly.
  • alkaline earth metals are referred to below as counterions for monovalent anions, this means that the alkaline earth metal is present only in half - as sufficient to charge balance - amount of substance as the anion.
  • This invention includes all conceivable types of detergents or cleaners, both concentrates and undiluted agents, for use on a commercial scale, in the washing machine or in hand washing. These include, for example
  • washing and cleaning agents for textiles, carpets or natural fibers, for which the term laundry detergent is used.
  • the washing and cleaning agents in the context of the invention also include washing aids, which in manual or machine textile washing to the actual
  • laundry detergents and cleaners in the context of the invention also include textile pre-treatment and post-treatment agents, ie those agents with which the laundry item is brought into contact before the actual laundry, for example to dissolve stubborn soiling, and also agents which are in one of the actual Textile laundry downstream step to give the laundry further desirable properties such as comfortable grip, crease resistance or low static charge.
  • textile pre-treatment and post-treatment agents ie those agents with which the laundry item is brought into contact before the actual laundry, for example to dissolve stubborn soiling
  • agents which are in one of the actual Textile laundry downstream step to give the laundry further desirable properties such as comfortable grip, crease resistance or low static charge i.a. calculated the fabric softener.
  • the washing or cleaning agents according to the invention may contain, in addition to the above-described cutinase, all known ingredients customary in such agents, preferably at least one further ingredient in the Means is present.
  • the agents according to the invention may in particular contain surfactants, builders, bleaches, in particular peroxygen compounds, or bleach activators.
  • they may contain water-miscible organic solvents, further enzymes, sequestering agents, electrolytes, pH regulators and / or further auxiliaries, such as optical brighteners, graying inhibitors, foam regulators, as well as dyes and fragrances, and combinations thereof.
  • Embodiments has improved cleaning performance by resulting synergisms.
  • a synergism by combining the agent according to the invention with a surfactant and / or a builder (builder) and / or a peroxygen compound and / or a bleach activator, such a synergism can be achieved.
  • Patent application WO2009 / 121725 starting there on page 5, penultimate paragraph, and ending on Page 13 after the second paragraph. This disclosure is incorporated herein by reference and the disclosure is incorporated herein by reference.
  • compositions according to the invention preferably also contain at least one compound from the class of surfactants, in particular selected from anionic and nonionic, but also cationic, zwitterionic or amphoteric surfactants.
  • Suitable surfactants are, for example, anionic surfactants of the formula (I)
  • R represents a linear or branched unsubstituted alkylaryl group.
  • Y stands for a monovalent cation or the n-th part of an n-valent cation, the alkali metal ions being preferred, and Na + or K + being preferred, Na + being extremely preferred.
  • Other cations Y + may be selected from NhV, Mn 2+ , and mixtures thereof.
  • Alkylaryl refers to organic radicals consisting of an alkyl radical and an aromatic radical Typical examples of such radicals include but are not limited to alkylbenzene radicals such as benzyl, butylbenzene radicals, nonylbenzene radicals, decylbenzene radicals, undecylbenzene radicals. Dodecylbenzene radicals, tridecylbenzene radicals and the like.
  • such surfactants are selected from linear or branched alkylbenzenesulfonates of the formula A-1 in which R ' and R " together contain from 9 to 19, preferably from 1 to 15, and in particular from 1 to 13, carbon atoms
  • R ' and R " together contain from 9 to 19, preferably from 1 to 15, and in particular from 1 to 13, carbon atoms
  • a particularly preferred representative can be described by the formula A-1a:
  • the compound of formula (I) is preferably the sodium salt of a linear alkyl benzene sulfonate.
  • the at least one compound from the class of anionic surfactants of the formula (I) is present in an amount of 0.001 to 30% by weight, preferably 0.001 to 10% by weight, more preferably 2 to 6% by weight. even more preferably 3-5% by weight, contained in the washing or cleaning agent, in each case based on the total weight of the cleaning agent.
  • compositions according to the invention preferably contain at least one anionic surfactant of the formula
  • R is a linear or branched, substituted or
  • unsubstituted alkyl, aryl or alkylaryl radical preferably a linear, unsubstituted alkyl radical, more preferably a fatty alcohol radical.
  • Preferred radicals R are selected from decyl, undecyl, dodecyl, tridecyl, tetradecyl, pentadecyl, hexadecyl, heptadecyl,
  • AO represents an ethylene oxide (EO) or propylene oxide (PO) moiety, preferably an ethylene oxide moiety.
  • n stands for an integer from 1 to 50, preferably from 1 to 20 and especially from 2 to 10. Most preferably, n stands for the numbers 2, 3, 4, 5, 6, 7 or 8.
  • X stands for a monovalent cation or the nth part of an n-valent cation, the alkali metal ions are preferred, and Na + or K + including Na, with Na + being extremely preferred.
  • Other cations X + may be selected from NhV, V Zn 2+ , 1H 2 Mg 2+ , 1 H Ca 2+ , 1 Mn 2+ , and mixtures thereof.
  • Particularly preferred representatives are Na-Ci2-14
  • the cleaning agent contains the at least one anionic surfactant of the formula (II) in an amount of 2-10% by weight, preferably 3-8% by weight, based on the total weight of the cleaning agent.
  • anionic surfactants are the alkyl sulfates of the formula
  • R 2 is a linear or branched, substituted or
  • unsubstituted alkyl radical preferably a linear, unsubstituted alkyl radical, more preferably a fatty alcohol radical.
  • Preferred radicals R 2 are selected from decyl, undecyl, dodecyl, tridecyl, tetradecyl, pentadecyl, hexadecyl, heptadecyl, octadecyl, nonadecyl, eicosyl radicals and mixtures thereof, the representatives having an even number of C Atoms are preferred.
  • radicals R 2 are derived from C 12-18 fatty alcohols, for example coconut oil fatty alcohol, tallow fatty alcohol, lauryl, myristyl, cetyl or stearyl alcohol or C 10 C 20 oxo alcohols.
  • Y stands for a monovalent cation or the nth part of an n-valent cation, the alkali metal ions being preferred, and Na + or K + being preferred, Na + being extremely preferred.
  • Other cations Y + may be selected from NhV,
  • these surfactants are selected from fatty alcohol sulfates of Formula A-3
  • the agent may contain, in addition to the anionic surfactants described above, in particular those of the formulas (I) - (III), or alternatively at least one other surfactant.
  • Suitable alternative or additional surfactants are in particular further anionic surfactants, nonionic surfactants and mixtures thereof, but also cationic, zwitterionic and amphoteric surfactants.
  • the agents comprise at least one nonionic surfactant, in particular at least one fatty alcohol alkoxylate.
  • Suitable nonionic surfactants are those of the formula
  • R 3 is a linear or branched, substituted or unsubstituted alkyl radical
  • AO for an ethylene oxide (EO) or propylene oxide (PO) grouping
  • n stands for integers from 1 to 50.
  • R 3 is a linear or branched, substituted or unsubstituted alkyl radical, preferably a linear, unsubstituted alkyl radical, particularly preferably a fatty alcohol radical.
  • Preferred radicals R 2 are selected from decyl, undecyl, dodecyl, tridecyl, tetradecyl, pentadecyl, hexadecyl, heptadecyl,
  • R 3 are derived from C 12 -C 18 -fatty alcohols, for example from coconut fatty alcohol, tallow fatty alcohol, lauryl, myristyl, cetyl or stearyl alcohol or from C 10 -C 20 oxo alcohols.
  • AO represents an ethylene oxide (EO) or propylene oxide (PO) moiety, preferably an ethylene oxide moiety.
  • EO ethylene oxide
  • PO propylene oxide
  • m is an integer from 1 to 50, preferably from 1 to 20 and especially from 2 to 10. Most preferably, m is the numbers 2, 3, 4, 5, 6, 7 or 8.
  • nonionic surfactants which may be included in the described compositions within the meaning of the present invention include, but are not limited to, alkyl glycosides, alkoxylated fatty acid alkyl esters, amine oxides, fatty acid alkanolamides, hydroxy mixed ethers,
  • Sorbitan fatty acid esters polyhydroxy fatty acid amides and alkoxylated alcohols.
  • Suitable amphoteric surfactants are, for example, betaines of the formula (R 1) XR 1 XR 3 N-chloroCOO-, in which R i is an alkyl radical having 8 to 25, preferably 10 to 21 carbon atoms interrupted by hetero atoms or heteroatom groups and R iv and R v are identical or different alkyl radicals 1 to 3 carbon atoms, in particular Cio-Cis-alkyl dimethylcarboxymethylbetain and Cn-Ci7-alkylamidopropyl-dimethylcarboxymethylbetain.
  • Suitable cationic surfactants are i.a. the quaternary ammonium compounds of the formula
  • R VI (R VI ) (R vii ) (R viii ) (R ix ) N + X "
  • R vi to R ix for four identical or different, in particular two long and two short-chain, alkyl radicals and X " for an anion , in particular a halide ion, for example, didecyldimethylammonium chloride, Alkylbenzyldidecylammoniumchlorid and mixtures thereof.
  • Suitable cationic surfactants are the quaternary surface-active compounds, in particular with a sulfonium, phosphonium, iodonium or
  • Arsonium group which are also known as antimicrobial agents.
  • the agent can be designed with an antimicrobial effect or its possibly existing antimicrobial effect due to other ingredients can be improved.
  • the total amount of the surfactants based on the weight of the composition is 2 to 30% by weight, preferably 5 to 25% by weight, more preferably 10 to 20% by weight, most preferably 14 to 18% by weight.
  • the (linear) alkylbenzenesulfonates at most in an amount of 0.001 to 30 wt .-%, preferably 0.001 to 10 wt .-%, more preferably 2 to 6 wt .-%, more preferably 3 - 5 wt .-%, based on the weight of the agent.
  • Detergents or cleaners according to the invention may contain other enzymes in addition to the cutinase. These may be hydrolytic enzymes or other enzymes in a concentration effective for the effectiveness of the agent.
  • One embodiment of the invention thus represents agents comprising one or more enzymes.
  • Preferred enzymes are all enzymes which can develop a catalytic activity in the agent according to the invention, in particular a protease, amylase, cellulase, hemicellulase, mannanase, tannase, xylanase, xanthanase, xyloglucanase, ⁇ -glucosidase, pectinase, carrageenase, perhydrolase, oxidase , Oxidoreductase or a lipase, and mixtures thereof.
  • Enzymes are in the middle
  • Each enzyme is increasingly preferred in an amount of 1 x 10 -3 ⁇ 7 wt .-%, of 0.00001-1 wt .-%, of 0.00005 to 0.5 wt .-%, from 0.0001 to 0, 1 wt .-% and particularly preferably from 0.0001 to 0.05 wt .-% in inventive compositions, based on active protein.
  • the enzymes show synergistic cleaning performance against certain stains or stains, ie the enzymes contained in the middle composition mutually support each other in their cleaning performance. Synergistic effects can occur not only between different enzymes, but also between one or more enzymes and other ingredients of the composition according to the invention.
  • the amylase (s) is preferably an ⁇ -amylase.
  • the hemicellulase is preferably a ⁇ -glucanase, a pectinase, a pullulanase and / or a mannanase.
  • the cellulase is preferably a cellulase mixture or a one-component cellulase, preferably or predominantly an endoglucanase and / or a cellobiohydrolase.
  • the oxidoreductase is preferably an oxidase, in particular a choline oxidase, or a perhydrolase.
  • the proteases used are preferably alkaline serine proteases. They act as nonspecific endopeptidases, that is, they hydrolyze any acid amide linkages that are located inside peptides or proteins and thereby cause degradation of proteinaceous soils on the items to be cleaned. Their pH optimum is usually in the clearly alkaline range.
  • the protein concentration can be determined by known methods, for example the BCA method (bicinchoninic acid, 2,2'-biquinolyl-4,4'-dicarboxylic acid) or the biuret method.
  • the determination of the active protein concentration takes place via a titration of the active sites using a suitable irreversible inhibitor (for proteases, for example phenylmethylsulfonyl fluoride (PMSF)) and determination of the residual activity (compare M. Bender et al., J. Am. Chem. Soc , 24 (1966), pp. 5890-5913).
  • a suitable irreversible inhibitor for proteases, for example phenylmethylsulfonyl fluoride (PMSF)
  • the enzymes to be used may also be formulated together with accompanying substances, for example from the fermentation.
  • accompanying substances for example from the fermentation.
  • the enzymes are preferably used as enzyme liquid formulation (s).
  • the enzymes are usually not provided in the form of the pure protein, but rather in the form of stabilized, storable and transportable preparations. To this
  • Prefabricated preparations include, for example, the solid preparations obtained by granulation, extrusion or lyophilization or, especially in the case of liquid or gel-form detergents, solutions of the enzymes, advantageously as concentrated as possible, low in water and / or added with stabilizers or further auxiliaries.
  • the enzymes may be encapsulated for both the solid and liquid dosage forms, for example by spray-drying or extruding the enzyme solution together with a preferably natural polymer or in the form of capsules, for example those in which the enzymes are entrapped as in a solidified gel or in those of the core-shell type, in which an enzyme-containing core with a water, air and / or
  • Chemical-impermeable protective layer is coated.
  • further active ingredients for example stabilizers, emulsifiers, pigments, bleaches or dyes, may additionally be applied.
  • Such capsules are applied by methods known per se, for example by shaking or rolling granulation or in fluid-bed processes.
  • such granules for example by applying polymeric
  • the agent according to the invention may comprise one or more enzyme stabilizers. Therefore, the agent according to the invention can also have a
  • Enzyme stabilizer for example, selected from the group consisting of sodium formate, sodium sulfate, lower aliphatic alcohols and boric acid and their esters and salts. Of course, two or more of these compounds may be used in combination become.
  • the salts of the compounds mentioned can also be used in the form of hydrates, such as, for example, sodium sulfate decahydrate.
  • lower aliphatic alcohols as used herein includes monoalcohols, diols, and higher alcohols having up to 6 carbon atoms.
  • polyols for example, glycerol, (mono) ethylene glycol, (Mono) Propylene glycol or sorbitol, without the invention being limited to these.
  • an agent of the invention may also contain at least one further stabilizer.
  • Such stabilizers are known in the art.
  • Reversible protease inhibitors protect the enzymes contained in a detergent or cleanser from proteolytic degradation by reversibly inhibiting the enzymatic activity of the proteases contained in the agent.
  • reversible protease inhibitors benzamidine hydrochloride, boronic acids or their salts or esters are frequently used, including in particular derivatives with aromatic groups, such as ortho, meta or para-substituted
  • Phenylboronic in particular 4-formylphenyl-boronic acid, or the salts or esters of said compounds.
  • peptide aldehydes that is oligopeptides with a reduced C-terminus, especially those of 2 to 50 monomers are used for this purpose.
  • peptidic reversible protease inhibitors include ovomucoid and leupeptin.
  • enzyme stabilizers are amino alcohols such as mono-, di-, triethanol- and -propanolamine and mixtures thereof, aliphatic carboxylic acids up to C12, such as succinic acid, other dicarboxylic acids or salts of said acids. End-capped fatty acid amide alkoxylates are also suitable for this purpose. Some organic acids used as builders can additionally stabilize an enzyme. Also calcium and / or magnesium salts are used for this purpose, such as calcium acetate.
  • Polyamide oligomers or polymeric compounds such as lignin, water-soluble vinyl copolymers or cellulose ethers, acrylic polymers and / or polyamides stabilize the enzyme preparation, inter alia, against physical influences or pH fluctuations.
  • Polyamine N-oxide-containing polymers act simultaneously as enzyme stabilizers and as
  • Dye transfer inhibitors Other polymeric stabilizers are linear Cs-ds polyoxyalkylenes. Also, alkylpolyglycosides can stabilize the enzymatic components of the agent according to the invention and, preferably, are capable of additionally increasing their performance.
  • networked N-containing compounds preferably perform a dual function as soil release agents and as enzyme stabilizers. Hydrophobic, nonionic polymer stabilizes in particular an optionally contained cellulase.
  • Reducing agents and antioxidants increase the stability of the enzymes to oxidative degradation;
  • sulfur-containing reducing agents are familiar, for example sodium sulfite and reducing sugars.
  • the compositions according to the present invention are liquid and contain water as the main solvent, i. they are aqueous agents.
  • the water content of the aqueous composition of the present invention is usually 15 to 70% by weight, preferably 20 to 60% by weight. In various embodiments, the water content is more than 5% by weight, preferably more than 15% by weight and particularly preferably more than 50% by weight, in each case based on the total amount of agent.
  • non-aqueous solvents may be added to the composition.
  • Suitable non-aqueous solvents include mono- or polyhydric alcohols, alkanolamines or glycol ethers, provided that they are miscible with water in the specified concentration range.
  • the solvents are preferably selected from ethanol, n-propanol, i-propanol, butanols, glycol, propanediol, butanediol, methylpropanediol, glycerol, diglycol, propyldiglycol, butyldiglycol, hexyleneglycol, ethylene glycol methyl ether, ethylene glycol ethyl ether,
  • Propylene glycol propyl ether dipropylene glycol monomethyl ether, dipropylene glycol monoethyl ether, methoxytriglycol, ethoxytriglycol, butoxytriglycol, 1-butoxyethoxy-2-propanol, 3-methyl-3-methoxybutanol, propylene glycol t-butyl ether, di-n-octyl ether and mixtures thereof
  • the one or more non-aqueous solvents is usually contained in an amount of 0.1 to 10% by weight, preferably 1 to 8% by weight, based on the total composition.
  • compositions according to the invention may contain further ingredients which further improve the performance and / or aesthetic properties of the cleaning agent.
  • additives for improving the flow and drying behavior for adjusting the viscosity and / or for stabilization, as well as other cleaning agents and additives customary in detergents, such as UV stabilizers, perfume, pearlescing agents, dyes, corrosion inhibitors, preservatives, bittering agents, organic Salts, disinfectants, structuring polymers, defoamers, encapsulated ingredients (eg encapsulated perfume), pH adjusters and skin feel-improving or nourishing additives.
  • An agent according to the invention in particular washing or cleaning agent, preferably contains at least one water-soluble and / or water-insoluble, organic and / or
  • inorganic builders builders
  • the builders which can generally be used are, in particular, the aminocarboxylic acids and their salts, zeolites, silicates, carbonates, organic (co) builders and, where there are no ecological prejudices against their use, also the phosphates.
  • the agents are phosphate-free.
  • the water-soluble organic builder substances include polycarboxylic acids, in particular citric acid and sugar acids, monomeric and polymeric aminopolycarboxylic acids, in particular methylglycinediacetic acid, nitrilotriacetic acid and ethylenediaminetetraacetic acid and also
  • Polyaspartic acid polyphosphonic acids, in particular Aminotris (methylenphosphonklare), ethylenediaminetetrakis (methylenephosphonic acid) and 1-hydroxyethane-1, 1-diphosphonic acid, polymeric hydroxy compounds such as dextrin and polymeric (poly) carboxylic acids, polymeric acrylic acids, methacrylic acids, maleic acids and copolymers thereof, which also small amounts of polymerizable substances without carboxylic acid functionality may contain polymerized.
  • Suitable, although less preferred, compounds of this class are copolymers of acrylic or methacrylic acid with vinyl ethers, such as vinylmethyl ethers, vinyl esters, ethylene, propylene and styrene, in which the acid content is at least 50% by weight.
  • vinyl ethers such as vinylmethyl ethers, vinyl esters, ethylene, propylene and styrene, in which the acid content is at least 50% by weight.
  • Organic builder substances can be used, in particular for the preparation of liquid agents, in the form of aqueous solutions, preferably in the form of 30 to 50 percent by weight aqueous solutions. All of the acids mentioned are generally used in the form of their water-soluble salts, in particular their alkali metal salts.
  • Organic builders may, if desired, be included in amounts of up to 40% by weight, more preferably up to 25% by weight, and preferably from 1% to 8% by weight. Quantities close to the stated upper limit are preferably used in paste-form or liquid, in particular water-containing, agents according to the invention.
  • Aftertreatment agents such as e.g. Softener, may optionally also be free of organic builder.
  • water-soluble inorganic builder materials are in particular alkali metal silicates and, if there are no concerns about their use, also polyphosphates, preferably
  • water-insoluble, water-dispersible inorganic Builder materials in particular crystalline or amorphous alkali metal aluminosilicates, if desired, in amounts of up to 50 wt .-%, preferably not more than 40 wt .-% and in liquid agents, in particular from 1 wt .-% to 5 wt .-%, are used , Among these are the detergent quality crystalline sodium aluminosilicates, especially zeolites A, P and
  • Suitable aluminosilicates have, in particular, no particles with a particle size greater than 30 ⁇ m, and preferably consist of at least 80% by weight of particles having a size of less than 10 ⁇ m.
  • Suitable substitutes or partial substitutes for the said aluminosilicate are crystalline alkali silicates which may be present alone or in a mixture with amorphous silicates.
  • the alkali metal silicates useful as builders in the compositions according to the invention preferably have a molar ratio of alkali metal oxide to SiO 2 below 0.95, in particular from 1: 1, 1 to 1: 12, and may be present in amorphous or crystalline form.
  • Preferred alkali metal silicates are the sodium silicates, in particular the amorphous sodium silicates, with a molar ratio of Na 2 O: SiO 2 of from 1: 2 to 12.8.
  • Crystalline silicates which may be present alone or in a mixture with amorphous silicates are preferably crystalline phyllosilicates of the general formula Na.sub.2SixO.sub.2.sup.x + H.sub.2O.sub.2, in which x, the so-called modulus, is a number from 1.9 to 4 and y is a number from 0 is up to 20 and preferred values for x are 2, 3 or 4.
  • Preferred crystalline phyllosilicates are those in which x in the abovementioned general formula assumes the values 2 or 3.
  • both beta- and delta-sodium disilicates Na 2 Si 2 O y H 2 O
  • beta- and delta-sodium disilicates Na 2 Si 2 O y H 2 O
  • amorphous alkali silicates practically anhydrous crystalline alkali silicates of the abovementioned general formula in which x is a number from 1, 9 to 2.1, can be used in inventive compositions.
  • a crystalline sodium layer silicate with a modulus of 2 to 3 is used, as can be prepared from sand and soda. Crystalline sodium silicates with a modulus in the range of 1.9 to 3.5 are used in a further preferred embodiment of compositions according to the invention.
  • the weight ratio of aluminosilicate to silicate is preferably 1:10 to 10: 1.
  • the weight ratio of amorphous alkali metal silicate to crystalline alkali metal silicate is preferably 1: 2 to 2: 1 and especially 1: 1 to 2: 1.
  • Builders are, if desired, in the inventive compositions preferably in amounts of up to 60 wt .-%, in particular from 5 wt .-% to 40 wt .-%, included. Particularly preferred are water-soluble builders in liquid formulations.
  • polymeric thickeners are the polycarboxylates which have a thickening effect as polyelectrolytes, preferably homopolymers and copolymers of acrylic acid, in particular acrylic acid copolymers such as acrylic acid-methacrylic acid copolymers, and the polysaccharides, in particular heteropolysaccharides, and other customary thickening polymers.
  • Suitable polysaccharides or heteropolysaccharides are the polysaccharide gums, for example gum arabic, agar, alginates, carrageenans and their salts, guar, guar gum, tragacanth, gellan, Ramzan, dextran or xanthan and their derivatives, e.g. propoxylated guar, as well as their mixtures.
  • Other polysaccharide thickeners such as starches or cellulose derivatives, may be used alternatively, but preferably in addition to a polysaccharide gum, for example starches of various origins and starch derivatives, e.g.
  • Hydroxyethyl starch starch phosphate esters or starch acetates, or carboxymethyl cellulose or its sodium salt, methyl, ethyl, hydroxyethyl, hydroxypropyl, hydroxypropylmethyl or hydroxyethyl methyl cellulose or cellulose acetate.
  • Acrylic acid polymers suitable as polymeric thickeners are, for example
  • acrylic acid copolymers are the following acrylic acid copolymers: (i) Copolymers of two or more monomers from the group of acrylic acid, methacrylic acid and their simple ester, preferably formed with C 1-4 alkanols (INCI acrylates copolymer), to which approx the copolymers of methacrylic acid, butyl acrylate and methyl methacrylate (CAS 25035-69-2) or of butyl acrylate and methyl methacrylate (CAS 25852-37-3); (ii) crosslinked high molecular weight acrylic acid copolymers, such as those crosslinked with an allyl ether of sucrose or pentaerythritol copolymers of Cio-30-alkyl acrylates with one or more monomers from the group of acrylic acid, methacrylic acid and their simple, preferably with Ci-4-alkanols formed esters (INCI acrylates / C 10-30 alkyl acrylate crosspolymer).
  • the content of polymeric thickener is usually not more than 8 wt .-%, preferably between 0.1 and 7 wt .-%, particularly preferably between 0.5 and 6 wt .-%, in particular between 1 and 5 wt .-% and most preferably between 1, 5 and 4 wt .-%, for example between 2 and 2.5 wt .-%, based on the total weight of the composition.
  • one or more dicarboxylic acids and / or salts thereof can be added, in particular a composition of Na salts of adipic, succinic and glutaric acid, as described, for example, in US Pat Trade name Sokalan ® DSC is available.
  • the use is advantageously carried out in amounts of 0.1 to 8 wt .-%, preferably 0.5 to 7 wt .-%, in particular 1, 3 to 6 wt .-% and particularly preferably 2 to 4 wt .-%, based on the total weight of the cleaning agent.
  • the detergents according to the invention can be compared with reference detergents in order to determine the increased anti-pilling performance of the compositions according to the invention.
  • Washing system can be composed as follows (all figures in weight percent):
  • Reference agent 4.4% alkylbenzenesulfonic acid, 5.6% other anionic surfactants, 2.4% C12-C18 Na salts of fatty acids (soaps), 4.4% nonionic surfactants, 0.2% phosphonates, 1, 4 %
  • Citric acid 0.95% NaOH, 0.01% defoamer, 2.0% glycerol, 0.08% preservatives, 1% ethanol, 1, 6% enzyme mix (protease, amylase, cellulase, mannase), balance demineralized water.
  • Composition according to the invention 4.4% of alkylbenzenesulfonic acid, 5.6% of other anionic surfactants, 2.4% of C12-C18 Na salts of fatty acids (soaps), 4.4% of nonionic surfactants, 0.2% of phosphonates, 1, 4% citric acid, 0.95% NaOH, 0.01% defoamer, 2.0% glycerol, 0.08% preservatives, 1% ethanol, 1, 6% enzyme mix (protease, amylase, cellulase, mannose), 0.009% cutinase , Rest demineralized water.
  • the dosage of the liquid detergent is between 4.5 and 6.0 grams per liter of wash liquor, for example, 4.7, 4.9 or 5.9 grams per liter of wash liquor. Preference is given to washing in a pH range between pH 8 and pH 10.5, preferably between pH 8 and pH 9.
  • the abovementioned embodiments of the present invention comprise all solid, powdery, liquid, gelatinous or paste-like administration forms of agents according to the invention which, if appropriate, may also consist of several phases and may be present in compressed or uncompressed form.
  • the agent can be present as a free-flowing powder, in particular with a bulk density of 300 g / l to 1200 g / l, in particular 500 g / l to 900 g / l or 600 g / l to 850 g / l.
  • the solid dosage forms of the composition also include extrudates, granules, tablets or pouches.
  • the agent can also be liquid, gelatinous or pasty, for example in the form of a non-aqueous liquid detergent or a non-aqueous paste or in the form of an aqueous liquid detergent or a water-containing paste.
  • the agent may be present as a one-component system. Such funds consist of one phase.
  • an agent can also consist of several phases, ie be, for example, one or two or more phases. Such an agent is therefore divided into several components (multi-component system).
  • Another object of the invention is a process for the cleaning of textiles, which is characterized in that in at least one process step, an inventive agent is applied.
  • the textiles preferably contain or consist of polyester.
  • the method described above is characterized in that the agent according to the invention at a temperature of 0-100 ° C, preferably 0-80 ° C, more preferably 30-70 and most preferably at 40-60 is used.
  • Methods for cleaning textiles are generally distinguished by the fact that various cleaning-active substances are applied to the items to be cleaned and washed off after the contact time, or that the items to be cleaned are otherwise treated with a detergent or a solution or dilution of this product. All conceivable washing or cleaning methods can be enriched in at least one of the method steps to the application of a washing or cleaning agent according to the invention and then represent embodiments of the present invention. All facts, objects and embodiments described for means according to the invention are also applicable to this subject of the invention , therefore, reference is made at this point expressly to the disclosure in the appropriate place with the statement that this disclosure also applies to the above inventive method.
  • a single and / or the only step of such a method is that the only cleaning active component is a cutinase with the Pollution is brought into contact, preferably in a buffer solution or in water.
  • the invention also encompasses the use of the agent described herein, for example as detergents or cleaners as described above, for the (improved) removal of stains, for example textiles, in particular polyester textiles.
  • the invention also relates to the use of a cutinase for reducing pilling effects of an agent, preferably a detergent, more preferably a liquid detergent, wherein the agent contains the cutinase.
  • the cutinase is a cutinase as defined herein.
  • the cutinase is present in an amount of 0.00001-1 wt%, preferably in an amount of 0.0001-0.5 wt%, more preferably in an amount of 0.001-0.1 Wt .-%, contained in the agent.
  • the cutinase which causes a reduction of the pilling effect, is applied to textiles, in particular textiles consisting of polyester or polyester.
  • the gene was made codon-optimized for expression in E. coli (SEQ ID NO: 3), and then cloned into the Ndel and Xho I sites of Novagen's PET-21b vector to form a fusion with C-terminal a 6x histidine tag is formed.
  • the sequencing-confirmed plasmid is transformed by conventional transformation protocols into electrocompetent cells E. coli BL21 (DE3) CodonPlus RIPL (Agilent Technologies).
  • the cultivation is carried out in LB medium at 37 ° C, with an induction with 0, 1 mM IPTG at an OD of 0.6 and then lowered to 16 ° C expression temperature.
  • the cells are harvested by centrifugation and resuspended in Lysis buffer (50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 20 mM Imidazole).
  • Lysis buffer 50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 20 mM Imidazole.
  • the cell disruption is carried out by means of ultrasound, followed by centrifugation (15,000 x g, 20 min, 4 ° C).
  • the supernatant contains the LC cutinase with HisTag.
  • NiNTA Purification via NiNTA (Qiagen, Hilden, DE) is carried out according to the protocol of the manufacturer Qiagen.
  • the degradation kinetics of the polyesterases described here were analyzed by QCM-D (quartz crystal microbalance with dissipation monitoring).
  • the preparation of the polyester model substrates was carried out from commercially available AT-Cut QCM-D crystals (Biolin Scientific) with a typical resonance frequency of 5 MHz and a gold-terminated electrode. By means of a spin coating process these crystals were modified with a polyester thin film.
  • Substrate PET film: 0.25 mm thick, amorphous, clear from GoodFellow Cambridge Limited, ES301445
  • PET film (0.5 cm x 2.5 cm) was washed with (1) 1, 7 mL 0, 1% SDS (w / v), 50 ° C, 30 min, 400 rpm; (2) 1, 5 mL of ethanol abs., 50 ° C, 5 min, 700 rpm; (3) Dried 1, 7 mL of double-distilled water, 50 ° C, 5 min, 700 rpm and 48 h at 50 ° C to constant and weighed. This was followed by incubation (48-72 h at 55 ° C.) with defined amount of cutinase according to SEQ ID NO: 1 (5, 25, 50 ⁇ g) in the total volume 1.7 mL in detergent matrix (see below, dosage 4.7 g / L).
  • the PET film was washed with (1) 1, 7 mL 0, 1% SDS (w / v) + 0.5% Triton X-100 (w / v), 50 ° C, 30 min, 400 rpm; (2) 1, 5 mL ethanol abs., 50 ° C, 5 min, 700 rpm, (3) 1, 7 mL double-distilled water, 50 ° C, 5 min, 700 rpm and 48 h at 50 ° C until Constance dried and weighed. The weight loss was determined.
  • the weight loss is given in comparison to the untreated film after 44 h incubation at 50 ° C.
  • the textiles to be assessed are various polyesters and mixed textiles, which are once new and once pre-pilled. After the 20 attempts, the
  • the pre-pilled tissues are washed 20 times at 40 ° C in
  • Sample 2 detergent + 4 mg cutinase (SEQ ID NO: 1) (according to the invention)
  • the cutinase according to the invention significantly improves the pill appearance.

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Detergent Compositions (AREA)

Abstract

L'invention concerne le domaine de la technologie enzymatique, en particulier l'effet antiboulochage des enzymes telles qu'utilisées par exemple dans des lessives ou des agents nettoyants. L'invention concerne un agent, en particulier une lessive ou un agent nettoyant renfermant une cutinase telle que définie dans le descriptif. L'invention concerne également un procédé pour nettoyer des textiles, ainsi que l'utilisation de l'agent selon l'invention pour supprimer des salissures. La présente invention se rapporte en outre à l'utilisation d'une cutinase telle que décrite ici pour réduire l'effet de boulochage dans un agent, de préférence une lessive ou un agent nettoyant.
EP18758569.0A 2017-08-24 2018-08-16 Propriétés d'entretien améliorées de textiles polyester ii Active EP3673035B1 (fr)

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DE102017214870.5A DE102017214870A1 (de) 2017-08-24 2017-08-24 Verbesserte Pflegeeigenschaften von Polyester Textilien II
PCT/EP2018/072173 WO2019038164A1 (fr) 2017-08-24 2018-08-16 Propriétés d'entretien améliorées de textiles polyester ii

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DE102018210609A1 (de) * 2018-06-28 2020-01-02 Henkel Ag & Co. Kgaa Polyesterase II
US11535832B2 (en) 2018-07-27 2022-12-27 Carbios Esterases and uses thereof
EP3699272A1 (fr) * 2019-02-21 2020-08-26 Henkel AG & Co. KGaA Propriétés de soin améliorées sur des textiles en polyester par l'utilisation d'un cutinase
CA3228057A1 (fr) * 2021-08-11 2023-02-16 Biometis Technology, Inc. Degradation enzymatique de polyethylene terephtalate
CN118871559A (zh) 2021-12-21 2024-10-29 诺维信公司 包含脂肪酶和加强剂的组合物
EP4630529A1 (fr) 2022-12-05 2025-10-15 Novozymes A/S Composition comprenant une lipase et un peptide

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AU710006B2 (en) 1995-04-28 1999-09-09 Genencor International, Inc. Detergents comprising cellulases
ES2554983T3 (es) * 2007-04-03 2015-12-28 Henkel Ag & Co. Kgaa Agente de lavado inhibidor del agrisado
DE102008017103A1 (de) 2008-04-02 2009-10-08 Henkel Ag & Co. Kgaa Wasch- und Reinigungsmittel enthaltend Proteasen aus Xanthomonas
CN102712879A (zh) * 2009-12-21 2012-10-03 丹尼斯科美国公司 含有褐色喜热裂孢菌脂肪酶的洗涤剂组合物及其使用方法
US20140187468A1 (en) * 2011-08-31 2014-07-03 Danisco Us Inc. Compositions and Methods Comprising a Lipolytic Enzyme Variant
WO2013096653A1 (fr) * 2011-12-22 2013-06-27 Danisco Us Inc. Compositions et méthodes comprenant un variant d'enzyme lipolytique
DE102014204374A1 (de) * 2014-03-11 2015-09-17 Henkel Ag & Co. Kgaa PET-Esterasen und deren Verwendung
DE102016208408A1 (de) * 2016-05-17 2017-11-23 Henkel Ag & Co. Kgaa Wasch- oder Reinigungsmittel enthaltend eine Polyethylenterephthalat Hydrolase
CA3030470A1 (fr) * 2016-07-12 2018-01-18 Carbios Esterases et utilisations connexes
US10590401B2 (en) * 2016-07-12 2020-03-17 Carbios Esterases and uses thereof

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