[go: up one dir, main page]

EP2735369B1 - Pipette multi-canal - Google Patents

Pipette multi-canal Download PDF

Info

Publication number
EP2735369B1
EP2735369B1 EP12007916.5A EP12007916A EP2735369B1 EP 2735369 B1 EP2735369 B1 EP 2735369B1 EP 12007916 A EP12007916 A EP 12007916A EP 2735369 B1 EP2735369 B1 EP 2735369B1
Authority
EP
European Patent Office
Prior art keywords
ejector
spigots
pipette
stop
pipette tips
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP12007916.5A
Other languages
German (de)
English (en)
Other versions
EP2735369A1 (fr
Inventor
Peter Schmidt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Priority to PL12007916T priority Critical patent/PL2735369T3/pl
Priority to EP12007916.5A priority patent/EP2735369B1/fr
Priority to US14/087,542 priority patent/US9339811B2/en
Priority to CN201310757368.8A priority patent/CN103990502B/zh
Priority to JP2013241905A priority patent/JP6141177B2/ja
Publication of EP2735369A1 publication Critical patent/EP2735369A1/fr
Application granted granted Critical
Publication of EP2735369B1 publication Critical patent/EP2735369B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/08—Ergonomic or safety aspects of handling devices
    • B01L2200/087—Ergonomic aspects

Definitions

  • the invention relates to a multi-channel pipette with a plurality of pins ("lugs") for clamping pipette tips and a discharge device for releasing pipette tips from the pins.
  • lugs pins
  • Pipettes are used in particular in medical, biological, biochemical, chemical and other laboratories for dosing liquids. These are picked up in pipette tips through a tip opening and dispensed.
  • a displacement device for air is integrated into the pipette and communicatively connected to the pipette tip through a connection hole of the pin. By means of the displacement device, an air cushion is displaced, so that liquid is sucked into the pipette tip and ejected therefrom.
  • the displacement device is usually a cylinder with a piston displaceable therein.
  • the pipette tips are releasably connected to the spigot so that they can be exchanged for a fresh pipette tip after use. As a result, contaminations are avoided at subsequent dosages.
  • Single-use pipette tips are available inexpensively from plastic.
  • the pin for holding pipette tips is also referred to as a "working cone” and is often a conical or cylindrical projection with respect to a housing or other body.
  • the pipette tip can be clamped onto the journal with a matching sealing seat at an attachment opening. This can be done without touching the pipette tip by pressing the pipette with the pin into the mounting opening of the pipette tip provided in a holder.
  • pipettes have a discharge device with a drive device and an ejector.
  • the drive device By actuating the drive device, the ejector is displaced so that it releases the pipette tip from the pin without the user having to touch it.
  • the drive device has a mechanism that must be actuated by means of a button to release the pipette tip from the pin.
  • the drive means has an electric motor that can be controlled by operating a knob to disengage the pipette tip from the pin.
  • manual pipettes i. for pipettes that can be held and operated by the user when using with one or both hands.
  • these pipettes have a mechanism for the displacement device which can be driven manually by means of a dosing button and, in the embodiment as electronic pipettes, an electric drive motor for the displacement device that can be controlled by means of an electrical dosing button.
  • Multi-channel pipettes serve to simultaneously receive liquid from one or more vessels or to dispense into one or more vessels.
  • Multichannel pipettes are widely used for the processing of microtiter plates which have a multiplicity of vessels in a matrix-like arrangement.
  • multi-channel pipettes have a plurality of parallel in a row next to each other at the same height arranged pin whose through holes each with a separate displacement means or a common Displacement device are connected.
  • the multiple displacement means and the common displacement means are connected in a manually driven multi-channel pipette with a mechanical drive means and in an electronic multi-channel pipette with an electric drive motor.
  • multichannel pipettes with an ejector are known, which presses off all the pipette tips from the pins with a rectilinear stop element and for this purpose has a drive mechanism which can be driven manually.
  • the force required to force several pipette tips off the spigots of a multichannel pipette is significantly greater than in the case of a single-channel pipette in which only one pipette tip is pressed off by one spigot.
  • Multi-channel pipettes are already known in which, in order to reduce the force required to eject the pipette tips, an ejector is not formed with a rectilinear stop element but with a stepped stop element.
  • the steps hit the pipette tips one at a time, requiring only the force to eject the pipette tips that are in contact with stages of the ejector.
  • the maximum force to be applied for the ejection of the pipette tips is thereby reduced.
  • a graduated stop element leads to different Aufsteck Toon the pipette tips on pins of a multi-channel pipette. At different Aufsteck Too reach not all pipette tips, which are simultaneously attached to the pins of the multichannel pipette, the bottoms of a microtiter plate.
  • US 5,021,217 and WO 98 / 20973A describe multi-channel pipettes with an ejector, in which the pins are arranged to attach pipette tips fixed to the housing.
  • the ejectors each have contact elements which protrude to varying degrees from the lower edge of Abwerferile. As a result, when displacing the ejector with the first contact element, a first group of pipette tips are pressed and then the other tips are pressed with the second contact element.
  • the DE 10 2004 003 433 B4 describes a multi-channel pipette, which reduces the force required for the operation of the ejection device in that it limits the force for clamping the pipette tips on the pins.
  • the multichannel pipette has a base body, a plurality of protruding from the body, axially movably mounted on the main body pin for placing pipette tips, displacement means which are fixedly connected to the pins, and springs, via which the displacement means are supported on the base body.
  • the sprung pin is associated with a stop over which the pins protrude axially when they are not loaded to the spring.
  • a discharge device for releasing the pipette tips from the pins has an ejector associated with the pins, wherein the pins and the ejectors are movable relative to each other.
  • the stop can also be the ejector.
  • the multi-channel pipette has a drive device operatively connected to the ejector and / or the pin for relatively moving the ejector and the pin.
  • the Aufklemmkraft is introduced into the springs. If the clamping force exceeds a certain value, the springs are elastically deformed until the pipette tips clamped on the pins strike against the stop. As soon as the pipette tips touch the stop, they can not be pushed further onto the pins. As a result, the clamping force of the pipette tips is limited.
  • the springs are designed and optionally biased, that the pipette tips exactly when they sit with the desired Aufklemmkraft on the pin, abut the stop. The clamping force is determined so that the pipette tips sit or seal securely on the pin.
  • the known pipette avoids high Aufklemm mechanism that complicate the ejection of the pipette tips.
  • the clamping force required for a secure fit and the sealing of the pipette tips on the pins must be ensured during the operation Throwing off be overcome.
  • the total applied Abwerfkraft is high because multiple pipette tips must be pressed simultaneously.
  • the present invention seeks to provide a multi-channel pipette, which further reduces the force required for the operation of the discharge device.
  • the clamping forces for clamping pipette tips are limited by the fact that the pins engage with the clamping forces acting on them during the clamping of pipette tips be shifted towards at least one stop element until the pipette tips impinge on the stop element, which is located in a defined stop position. If the multi-channel pipette has several stop elements, they are in the stop position at the same height.
  • the first spring elements are designed so that the pipette tips strike the stop element when defined clamping forces are achieved. These are preferably chosen so that the pipette tips sit securely and sealingly on the pin. High Aufklemm technique that would have to be overcome when dropping the pipette tips are avoided.
  • the use of an ejector with arranged in stages stop elements would not make sense, because this geometry favors different Aufsteck Toon the pipette tips, which are associated with different Aufklemm mechanismn.
  • the multichannel pipette according to the invention nevertheless makes it possible to clamp a plurality of pipette tips with a defined clamping force and to reduce the maximum force for ejecting the pipette tips.
  • the ejector is designed so that when displacing the ejector down at least after the impact of a first contact element on pipette tips a second contact element lags the first contact element.
  • a second contact element lags the first contact element.
  • first of all one or more pipette tips are pressed off the pins by the first contact element, and only one or more pipette tips are then pressed away from the pins by the second contact element.
  • the maximum force for the ejection of the pipette tips is also reduced in the multi-channel pipette according to the invention.
  • the multi-channel pipette only has first and second contact elements on first and second Abwerferieri.
  • the invention includes embodiments which has more than two Abwerferwel each with at least one contact element, wherein the contact element of another (eg third) Abwerferteils the contact element of the preceding (eg second) Abwerferteils at least after hitting pipette tips.
  • the multi-channel pipette has identically designed pins, which are arranged in their initial position at the same height.
  • the pins are preferably formed in accordance with the shape and dimensions coincident.
  • the first spring elements of the multi-channel pipette are designed consistently.
  • the first spring elements are biased when the pins are in the starting position.
  • the displacement device is a piston-cylinder device, wherein the displacement chamber is a cylinder and the displacement member is a piston displaceable in the cylinder.
  • the first and second Abwerferwel plate-shaped the first contact element is at least one projecting horizontally from the lower edge of the first Abwerferteils first ridge with one or more first through holes
  • the second contact element is at least one horizontally from the lower edge of the second Abwerferteils projecting second web with one or more second through holes
  • the pins pass through the first and second through holes.
  • the ejection force can be applied to one or more pipette tips on which the first contact member is seated, and one or more pipette tips, which is subsequently seated on the second contact element.
  • This embodiment is the ejector in a narrow, box-shaped pipette base (also called "dispensing head") of the multi-channel pipette housed.
  • the ejector comprises a first ejector part, which is connected to the drive device, and a second ejector part, wherein the first ejector part and the second ejector part are displaceably guided by means for guiding in the longitudinal direction of the pins, are the first Abwerferteil arranged with the first contact element and the second Abwerferteil with the second contact element in the starting position of the ejector at the same height and first driving means on the first Abwerferteil and second entrainment means on the second Abwerferteil available, which are spaced apart in the initial position of the ejector and the displacement of the Abwerfers down by moving the first Abwerferteils and the second Abwerferteils relative to each other along the means for guiding against each other are movable to each other on the impact of the first and second entrainment means the second Abwerferteil concurrent with the to shift down the first ejector part.
  • the first and second driving means of the ejector are at a distance from each other.
  • the first ejection part and the second ejection part are displaceable relative to one another in the longitudinal direction of the pins.
  • the displacement of Abwerferile with respect to each other is limited by the impact of the first and second entrainment means on each other.
  • the first Abwerferteil is displaced relative to the second Abwerferteil until the first entrainment means on the take second driving means. Thereafter, the first Abwerferteil takes the second Abwerferteil down with the second contact element pushes pipette tips of the pins.
  • the first Abwerferteil takes the second Abwerferteil down with the second contact element pushes pipette tips of the pins.
  • the contact elements are in the starting position of the ejector at the same height.
  • the contact elements can also be used as stop elements.
  • the contact elements are at the same time the stop elements and take the contact elements in the starting position of the ejector, the stop position.
  • the distance between the first and second driving means in the starting position of the ejector in the vertical direction is 0.1 to 3 mm, preferably 0.5 to 1.5 mm. This distance is sufficient to press different pipette tips or different group of pipette tips by means of the ejector successively and can be realized without noticeably increasing the discharge stroke.
  • the first ejector part has at its lower edge a first recess with first guide elements on two lateral edges, and the second ejector part is inserted into the first recess of the first ejector part and on second guide elements on two lateral edges on the first guide elements of first Abwerferteil in the longitudinal direction of the pins displaced.
  • the first and second ejection parts are preferably plate-shaped.
  • the first entrainment means are formed by the upper edge of the recess and the second entrainment means from the upper edge of the second Abwerferteils.
  • the second Abwerferteil is displaced into the recess until it meets with its upper edge on the upper edge of the depression.
  • the second ejection part is held on the first ejection part by connecting means which have a play in the longitudinal direction of the pin.
  • the connecting means are snap-connection means. The connection of the Abwerfermaschine by (snap) connection means is advantageous for assembly.
  • the snap connection means comprise an eyelet on the first ejector part and a catch hook engaging the eyelet with play in the longitudinal direction of the eyelet on the second ejection part or vice versa.
  • the first ejector part and the second ejector part preferably each consist of a rigid material. Further preferably, they are made of a plastic, further preferably of a thermoplastic.
  • the ejector has a rigid first ejector part and an at least partially soft second ejector part and are the first ejector part with the first contact element and the second ejector part with the second contact element in the starting position of the ejector arranged at the same height.
  • the contact elements are in the starting position of the ejector at the same height, they are also available as stop elements. According to a preferred further embodiment, the contact elements are at the same time the stop elements and take the contact elements in the starting position of the ejector, the stop position.
  • the second Abwerferteil is according to a configuration on both sides - for example, by columns or slots - separated from the first Abwerferteil.
  • the elastic compression of the second Abwerferteils is not hindered by a connection with the first Abwerferteil on the two longitudinal sides of the second Abwerferteils.
  • the second Abwerferteil is elastic overall.
  • the second Abwerferteil is soft elastic in only one or more sections and otherwise rigid.
  • the soft elastic portion preferably extends parallel to the second contact element, so that the second Abwerferteil is elastically compressed when pressing the second contact element against the upper ends of pipette tips. The elastic deformation of the second ejection member concentrates on the soft elastic portion of the second ejection member.
  • the second contact element is a soft-elastic portion of the second Abwerferteils.
  • the second contact element is formed on a rigid portion of the second Abwerferteils and has the second Abwerferteil the soft elastic portion at a distance from the second contact element.
  • the second Abwerferteil is connected via a soft elastic portion with the rigid first Abwerferteil or supported on this.
  • the rigid portion of the second Abwerferteils below the soft elastic portion of the second Abwerferteil is guided according to a further embodiment by means for guiding in the longitudinal direction of the pin on the first Abwerferteil displaced.
  • the rigid portion of the second removal part is displaceably guided on second guide elements on two lateral edges on first guide elements of the first ejection part in the longitudinal direction of the pins.
  • the second ejection part is in a depression at the bottom Arranged edge of the first Abwerferteils, connected at its upper edge via a soft elastic portion fixed to the upper edge of the recess.
  • the at least partially soft elastic second ejection member is connected according to an embodiment at its upper edge via a snap connection or via another detachable or non-detachable connection with the first Abwerferteil.
  • the first Abwerferteil consists of a thermoplastic and / or the second Abwerferteil total or at least partially made of a thermoplastic elastomer, a silicone elastomer or latex.
  • the second Abwerferteil partially made of a thermoplastic.
  • a second ejection member having at least one soft elastic portion and at least one rigid portion is preferably made of two different plastic components in the two component or in the multi-component injection molding process.
  • the second Abwerferteil is connected via a soft elastic portion with the first Abwerferteil.
  • the fixedly connected second and first Abwerfermaschine can be prepared by two-component or multi-component injection molding.
  • the means for displaceably supporting the ejector have at least one guide slot extending in the longitudinal direction of the pin in the ejector and at least one guide element engaging in the guide slot and firmly connected to the base body.
  • the Guide element is preferably a pin, a rib or another projection which engages in the guide slot.
  • the ejector is in the starting position with the first and second contact elements or another Abwerferteil on a fixedly arranged on the main body counter-stop element, which prevents the ejector at a further displacement upwards. This ensures that the ejector is not displaced beyond its initial position to the top. If the contact elements are also stop elements, the counter-impact element supports the ejector, so that the contact elements are arranged in the stop position.
  • the home position of the ejector may be defined by guide slots in the first and second ejector parts and in the guide slots engaging pins, ribs or other protrusions which abut in the home position at the bottom of the slots.
  • the counter-stop element is rigid.
  • the counter-stop element is a lower wall of the base body with third passage openings through which the pins project downwardly and against which the contact elements in the starting position lie down.
  • the counter-stop element may in particular be formed by a lower wall of a housing of the multichannel pipette. In the lower wall and the first and second contact elements high forces can be introduced when clamping pipette tips.
  • the at least one stop element is formed separately from the contact elements of the ejector.
  • the stop element is fixedly arranged in the defined stop position on the base body.
  • the stopper member is formed by a lower wall of the main body having third through holes through which the pins project downwardly.
  • the bottom wall extends only to a horizontal line which intersects all the pins so that the third through holes only partially surround the pins.
  • the third passages surround the pegs only in half, or up to a horizontal line that intersects all the pegs in the center.
  • the ejector with its contact elements is arranged at least at the same height as this.
  • the contact elements on first and second through holes which partially surround the pins on one side.
  • the ejector When the ejector is in the home position, pipette tips are clamped onto the pins until they impact the contactor. To eject the pipette tips, the ejector is displaced downwards, so that first the first contact elements and then the second contact elements and, if appropriate, then further contact elements press off pipette tips from the pins.
  • the ejector may be formed with contact elements which are arranged rigidly in stages. Instead, the ejector relative to each other displaceably guided Abwerferwel or have a rigid and a soft-elastic Abwerferteil.
  • the ejector is displaceable downward against the spring action of a second spring element acting on the ejector and on the main body.
  • the second spring element is biased when the ejector is in the starting position.
  • the multi-channel pipette has means for releasably holding the ejector in the starting position.
  • the means for releasably holding are, for example latching means which the ejector in the starting position hold.
  • the ejector is eg by clamping pipette tips on the pins of the pipette tips from a lower end position displaced to the starting position, in which the ejector is held by the means for releasable holding.
  • the number of pins, of which by means of the first contact element pipette tips are abdschreibbar the same size as the number of pins which are abdschreibbar by which means of the second contact element pipette tips.
  • the ejection forces are distributed evenly on the first ejector part and the second ejector part.
  • the multichannel pipette has any number of pins for simultaneously holding any number of pipette tips.
  • the multi-channel pipette has eight pins or twelve pins.
  • the multi-channel pipette on a multiple of eight pins or a multiple of twelve pins.
  • the pins of the multi-channel pipette are preferably arranged in a row next to each other.
  • the multi-channel pipette has a plurality of rows of pins arranged next to one another.
  • pipette tips of in each case four pins or of in each case six pins can be pressed off by means of the first and the second contact element.
  • These multichannel pipettes can hold a total of eight pipette tips or twelve pipette tips simultaneously. This is advantageous for use with common microtiter plates having recesses in eight rows and twelve columns.
  • pipette tips of in each case less than four pins or of more than six pins can be pressed off by means of the first and the second contact element.
  • These Embodiments may be particularly advantageous multi-channel pipettes having less than eight pins or more than twelve pins.
  • the multichannel pipette is a manual pipette, i. a pipette that can be held and operated by the user when using one or both hands.
  • the first drive device is mechanical in a manually driven pipette. It preferably has a dosing button and a lifting rod protruding from the dosing button, which is connected or detachable at the lower end with the displacement element of the displacement device.
  • the multichannel pipette is a fixed volume pipette or a pipette with adjustable dosing volume. In a fixed-volume pipette, the stroke of the lifting rod is limited by impingement of a circumferential bead or other projection on the circumference of the lifting rod to an upper and a lower stop whose position is not adjustable in the main body. In a pipette with adjustable dosing volume, the position of at least one of the two stops in the body is adjustable.
  • the multi-channel pipette for displacing the upper stop on a threaded spindle, which is threadably arranged in a nut which is fixedly connected to the main body.
  • the lifting rod is passed through a passage channel of the threaded spindle and the lower end face of the threaded spindle forms the upper stop for the projection on the lifting rod.
  • the second drive device can be actuated by means of the dosing button of the first drive device. After a Dosierhub and an overstroke the pipette tips are dropped in a discharge stroke of Dosierknopfes.
  • This embodiment allows a dosing and ejection of pipette tips by pressing the same button (so-called “one-button operation").
  • the multichannel pipette has a second drive device with a discharge button, which is formed separately from the dosing button (so-called "two-button operation").
  • a trained manually driven multi-channel pipette is for example in the DE 10 2004 003 433 B4 described.
  • the multichannel pipette has an electric drive motor and a transmission between the drive motor and the displacement element and an electronic control of the drive motor with an electrical dosing button.
  • the second drive means of the multi-channel pipette is designed, for example, as in the DE 10 2004 003 433 B4 described.
  • the multi-channel pipette comprises a pipette top comprising the first and second drive means and a dispensing head comprising the displacement chambers and the ejector.
  • the pipette top and the dispensing button are preferably releasably connectable to each other.
  • the means for detachably connecting pipette upper parts and dispensing head are for example designed as in the DE 10 2004 003 433 B4 described.
  • the terms “up” and “down”, “at the same height” and “horizontal” and “vertical” refer to an orientation of the multichannel pipette in which pipette tips clamped on the pegs are vertically aligned with their syringe orifices facing downwards to pick up a liquid from an underlying vessel or to dispense into the vessel.
  • All embodiments relate to multi-channel pipettes in which the contact elements of the ejector are also stop elements.
  • the contact elements are hereinafter referred to as stop elements.
  • a multichannel pipette 1.1 has a pipette top part 2 with an upper housing part 3 and a pipette lower part 4 with a lower housing part 5.
  • the upper housing part 3 is designed as a handle or a handle-shaped one.
  • the lower housing part 5 essentially has the shape of a flat box.
  • Upper housing part 3 and lower housing part 5 are together a basic body.
  • the pipette upper part 2 comprises a first drive device 6 for displacement devices and a second drive device 7 for a ejector.
  • the first drive device 6 comprises a top of the housing upper part 3 projecting and longitudinally displaceable in the upper housing part arranged Dosierknopf 8.
  • the Dosierknopf 8 is coupled at the bottom with a lifting rod 9, which has a circumferential bead 10 on the circumference.
  • the lifting rod 9 is in her Longitudinal direction by pressing the Dosierknopfes 8 against the action of a return spring 11 in the upper housing part displaced.
  • the displacement of the lifting rod 9 is limited by an upper stop 12 and a lower stop 13.
  • the upper stop 12 is adjustable in the longitudinal direction of the upper housing part 3.
  • the Dosierknopf 8 is coupled via a gear 14 with the upper stop 12, so that by turning the Dosierknopfes 8, the upper stop 12 in the longitudinal direction of the upper housing part 3 is adjustable.
  • the lower stop 13 is held in the upper housing part 3 by a spring device 15.
  • the spring means 15 also called “overstroke spring” allows for the impact of the bead 10 on the lower stop 13, a further displacement of the lifting rod 9 under increased effort down to perform an overstroke and a discharge stroke.
  • a transmission device 16 is arranged in the upper housing part 3, which transmits a displacement of the lifting rod 9 during the discharge stroke to a displacement of a discharge sleeve 17 downwards, which is arranged at the lower end of the housing upper part 3.
  • the upper housing part 3 has at the lower end a hollow cylindrical receptacle 18 into which a sleeve-shaped retaining element of the pipette lower part 4 can be inserted through an insertion opening 19 in the lower end.
  • the pipette upper part 2 has on the receptacle 18 first means for detachable connection 20 with the pipette lower part 4.
  • the ejection sleeve 17 is arranged concentrically with respect to the receptacle 18 and projects from the lower edge of the insertion opening 19 downwards.
  • the lower end of the lifting rod 9 projects from above into the receptacle 18 when the dosing button 8 is not pressed.
  • the pipette lower part 4 comprises a lower housing part 5, which is formed by a front and a rear housing shell 21, 22, which are joined together in a vertical plane.
  • the lower housing part 5 eight parallel piston-cylinder devices 23 are arranged in a row.
  • Each piston-cylinder device 23 has a cylinder 24 into which a piston 25 dips.
  • each cylinder 24 is integrally connected to a pin 26, which tapers downwardly, to a metering member 27.
  • Each pin 26 has a connection hole 28 which is connected at the top to an inner space of the cylinder 24 in which the piston 25 is displaceable, and opens at the bottom in an opening 29 in the lower end of the pin 26.
  • each metering component 27 has a circumferential projection 30.
  • the circumferential projections 30 of the Dosierbaumaschine 27 are based on the upper edges of third through holes 31 in a horizontal bottom wall 32 of the housing base 5 from.
  • the cylinders 24 are passed further up through fourth through-holes 33 of a horizontal support plate 34 of the housing base 5.
  • each cylinder 24 designed as a helical spring first spring element 35 is guided, which is supported on the bottom of the circumferential projection 30 and the top of the underside of the support plate 34.
  • the pistons 25 have at the upper end of a piston rod 36 a piston plate 37.
  • the piston plates are in piston plate receptacles 38 a horizontally oriented Traverse 39 held.
  • the traverse 39 has a vertically upstanding rod 40 at the top, which has a contact surface for the lower end of the lifting rod 9 of the pipette shell 2 at the upper end.
  • the holding element 41 has second means for releasably connecting 42, which are detachably connected to the first means for releasably connecting 20 of the pipette top 2.
  • the pipette lower part 4 comprises an ejector 43.
  • the ejector 43 has a plate-shaped substantially rectangular, first Abwerferteil 44 with chamfers 45, 46 at the two upper corners.
  • the first Abwerferteil 44 has a rectilinear, lower edge 47 a centrally disposed, rectangular first recess 48.
  • the first Abwerferteil 44 has at the lower edge 47 on both sides of the recess 48 each have a first stop member 49, 50, which by a horizontally oriented first web is formed, the first through holes 51, 52 which are open to the front edge 53, 54 of the first stop member 49, 50 out.
  • first Abwerferteil 44 a plurality of parallel guide slots 55, which are aligned perpendicular to its lower edge 47.
  • the first ejector portion 44 has centrally at the upper first edge 56 of the first recess 48 a downwardly projecting eyelet 57 with an elongate, rectangular eyelet opening 58.
  • an actuator 59 projects in the form of a shaft having a flat, aligned with the front of the first Abwerferteils front and with respect to the back of the first Abwerferteils 44 slightly cylindrical from the bulging back.
  • the actuator 59 has a longitudinally extending groove 60 which is bounded above by a first abutment 61.
  • the actuator 59 has two above, strip-shaped transmission elements 62, which are hollow cylindrical in a horizontal section.
  • a plate-shaped second Abwerferteil 63 is inserted in the first recess 48.
  • the second ejector portion 63 has at the lower edge 64 a second stop member 65 formed by a second land having second through holes 66 opened to the front edge 67 of the second land.
  • a small second recess 69 is provided, projecting beyond which a snap hook 70 which is connected below the second recess 69 with the front of the second Abwerferteils 63.
  • the upper first edge of the first recess 48 is a first entrainment means 56 and the upper second edge of the second Abwerferteils 63, a second driving means 68.
  • a gap 71 is present, which is for example about 0.75 mm.
  • the snap hook 70 is located at the bottom of the eyelet opening 58.
  • the second Abwerferteil 63 has at the two lateral edges in each case a longitudinal groove 72, 73.
  • the first Abwerferteil 44 engages with the two lateral edges 74, 75 of the first recess 48 in the two longitudinal grooves 72, 73 a.
  • the ejector 43 is guided in the rear housing shell 22 of the lower housing part 5 of ribs 76 which protrude from the inside of the rear wall 77 of the housing lower part 5.
  • the ribs 76 engage in the guide slots 55.
  • the actuator 59 is passed with the transmission elements 62 through arcuate slots 78 in a horizontal upper wall 79 of the rear housing shell 22.
  • the rear wall 77 of the rear housing shell 22 has an outwardly curved rear wall part, in which the actuating element 59 is inserted with its bulging side.
  • the rear wall 77 has on the inside in the region of the bulge on a second abutment.
  • a second spring element 82 designed as a helical spring is held in the groove 60 under prestressing, which loads the ejector 43 so that the ribs 76 bear against the lower end of the guide slots 55.
  • Two side walls 83, 84 of the rear housing shell 22 have on the inside small projecting retaining elements 86, which project slightly beyond the lateral edges of the first Abwerferteils 44.
  • the first ejector portion 44 has two small lateral recesses 87, 88. The first ejector portion 44 is pushed with the side recesses 87, 88 on the retaining elements 86 and is held by the second spring element 82 in a position in which the retaining elements 85, 86 below the lateral recesses 44 abut the first Abwerferteil 44.
  • the ejector is thus held up in the slots 78 and at the bottom by the retaining elements 85, 86 in the rear housing shell 22.
  • the first and second stopper members 49, 50, 65 abut the bottom wall 32 of the housing base 5 at the bottom and the transmission members 62 abut against the retaining member 41 on the outside.
  • the lifting rod 9 is located at the bottom of the upper end of the rod 40.
  • the ejection sleeve 17 abuts against the upper end of the transmission elements 62.
  • the multi-channel pipette 1.1 is inserted with the pins 26 in the Aufstecgro réelleen of pipette tips 89, which are provided in a holder.
  • the first and second stop members 49, 50, 65 of the ejector 43 in stop position at the same height, as in Fig. 1 . 2 and 9 shown.
  • the first spring elements 35 are compressed until the pipette tips 89 rest against the first and second stop elements 49, 50, 65 at the bottom.
  • the multi-channel pipette 1.1 is lifted with the clamped pipette tips and the pins 26 return to the starting position of Fig. 1 . 2 and 9 back.
  • the Dosierknopf 8 is pressed until the impact of the bead 10 on the lower stop 13, an increased resistance is felt.
  • the lifting rod 9 moves over the rod 40 and the cross member 39, the piston 25 against the action of the first spring elements 35 down so that air is forced out of the pipette tips.
  • the pipette tips are simultaneously immersed in vessels and then the Dosierknopf 8 relieved.
  • the return spring 11 displaces the lifting rod 9 back into the starting position and the first spring elements 35 displace the pistons 25 back into the starting position. As a result, liquid is sucked from the vessels into the pipette tips 89.
  • the pipette tips 89 are directed to other vessels.
  • the dosing button 8 is pressed until the impact of the bead 10 on the lower stop 13.
  • an overstroke for a residual quantity is initially effected.
  • the end of the overstroke is felt by the user at a further increased resistance due to the action of the second spring element 82 at the beginning of the discharge stroke.
  • a discharge stroke is effected, in which the transfer device 16, the discharge sleeve 17 shift down.
  • the ejection sleeve 17 moves the transmission elements 62 down and the ejector 43 is pressed against the action of the second spring member 82 down.
  • the return spring 11 presses the dosing button 8 via the lifting rod 9.
  • the return spring 11 also pushes the transfer device 16 ejection sleeve 17 back into the upper position.
  • the transfer device 16 has its own return spring, which moves the transfer device 16 and the discharge sleeve 17 back into the upper position.
  • the spring device 15 assumes its initial state again.
  • the second spring element 82 displaces the ejector 43 in the starting position of Fig. 1 . 2 and 9 back.
  • the multichannel pipette 1.2 of Fig. 11 and 12 differs from the above-described in that it has a two button operation.
  • the two-button operation of the dosing 8 controls only the recording and the delivery of liquid and controls an additional ejection button 90 the ejector 43.
  • the discharge rod 91 is in turn connected to the bottom of the discharge sleeve 17.
  • a discharge spring 92 engages the discharge bar 91 or the discharge button 90 and a fixed point in the upper housing part 3, so that the discharge button 90 against the action of the ejector spring 92 can be pressed.
  • this multichannel pipette 1.2 differs from the operation of the previously described multichannel pipette 1.1 only in that for dropping the pipette tips 89 of the ejection button 90 must be pressed. As a result, the ejection sleeve 17 is displaced downwards, whereby the transmission elements 62 of the ejector 43 are displaced downwards.
  • the ejection spring 92 sets the ejection button 90 and the ejection rod 91 back to the upper starting position. Further, the second spring element 82, the ejector 43 in the dispensing head 4 back to the starting position.
  • the ejector 43.1 of Fig. 13 and 14 differs from the previously described ejector 43 in that at the upper second edge 68 of the second Abwerferteils 63, a strip-shaped portion 93.1 molded of soft elastic material.
  • the strip-shaped portion 93.1 abuts with its upper edge on the upper first edge 56 of the first Abwerferteils 44.
  • the second Abwerferteil 63 is held by the snapped in the eyelet 57 snap hook 70 free of play.
  • the ejector 43.1 is like the previously described ejector 43 in the previously described multi-channel pipette 1.1, 1.2 can be installed.
  • the ejector 43 When attaching and dropping pipette tips 89 on and from the pins 26 of pipette tips 89 on and from the pin 26 of the ejector 43.1 acts as the ejector 43.
  • the strip-shaped portion 93.1 When ejecting the pipette tips on the pipette tips 89, the strip-shaped portion 93.1 is first compressed in the vertical direction until the spring force acting in the strip-shaped section 93.1 is high enough to push the middle group of pipette tips 89 away from the pins 26.
  • the material consisting of rigid material lower plate-shaped portion 94 of the second Abwerferteils 63 guided with the longitudinal grooves 72, 73 at the lateral edges 74, 75 of the first Abwerferteils 44 (corresponding to Fig. 5 ).
  • the ejector 43.2 of Fig. 15 and 16 differs from the ejector 43 in that the second Abwerferteil 63 has a strip-shaped portion 93.2 of soft elastic material at the lower edge 64.
  • the strip-shaped portion 93. 2 leaves the second through-holes 66 exposed, so that the pins 26 can be passed through the second through-holes 66.
  • the strip-shaped section 93.2 forms the second stop element 65 and at the same time the second contact element.
  • the second ejection part 63 is formed integrally with the first ejection part 44.
  • the ejector 43.2 is like the ejector 43 in a multi-channel pipette 1.1, 1.2 can be installed.
  • the ejector 43. 2 acts like the ejector 43.
  • the strip-shaped section 93. 2 is first compressed when it strikes the middle group of pipette tips 86.
  • the strip-shaped section 93.2 or the second ejector 43.1 pushes the middle group of pipette tips away from the pins 26.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Claims (16)

  1. Pipette multi-canal avec
    • un corps de base (5),
    • plusieurs tourillons (26) disposés l'un à côté de l'autre parallèlement en une ligne, faisant saillie du corps de base (5), logés de manière mobile dans leur sens longitudinal sur le corps de base, pour le serrage de pointes de pipette (89),
    • au moins un dispositif de refoulement (23) avec une chambre de refoulement (24) et un organe de refoulement (25) pouvant être déplacé à l'intérieur de cette dernière, dans lequel la chambre de refoulement (24) est reliée à des trous de connexion (28) dans les tourillons (26) afin d'évacuer ou d'aspirer de l'air à travers des orifices (29) des trous de connexion (28) se trouvant aux extrémités inférieures des tourillons (26),
    • un premier dispositif d'entraînement (6) relié à l'organe de refoulement (25) et formé de manière à déplacer l'organe de refoulement (25) dans la chambre de refoulement (24),
    • des premiers éléments de suspension (35) prenant sur les tourillons (26) et sur le corps de base (5), les tourillons (26) pouvant être déplacés, de manière à s'opposer à l'effet de ressort des premiers éléments de suspension (35), d'une position initiale dans leur sens longitudinal vers le haut,
    • au moins un élément de butée (49, 50, 65) qui a une position de butée définie, où, dans le cas de plusieurs éléments de butée (49, 50, 65), ces derniers se trouvent en position de butée au même niveau où les tourillons (26) font saillie vers le bas de l'élément de butée (49, 50, 65),
    • dans laquelle les premiers éléments de suspension (35) sont conçus de manière à ce que les tourillons (26) puissent être déplacés vers l'élément de butée (49, 50, 65) en position de butée par des forces de serrage définies pouvant être appliquées par serrage de pointes de pipette (89) sur les tourillons (26) de manière à ce que les pointes de pipette (89) touchent l'élément de butée (49, 50, 65), et
    • un dispositif d'éjection pour le détachement de pointes de pipette (89) des tourillons (26), comprenant un éjecteur (43) présentant des éléments de contact (49, 50, 65), des moyens de logement mobile (55, 76) de l'éjecteur (43) sur le corps de base (5) dans le sens longitudinal des tourillons (26) et un dispositif d'entraînement relié à l'éjecteur (43), dispositif d'entraînement formé pour déplacer l'éjecteur d'une position initiale, à laquelle des pointes de pipette (89) peuvent être serrées jusqu'au contact avec l'élément de butée (49, 50, 65) à la position de butée sur les tourillons (26), vers le bas dans le sens longitudinal des tourillons (26) afin de détacher des pointes de pipette (89) des tourillons (26), par pression, avec les éléments de contact (49, 50, 65),
    • dans lequel l'éjecteur (43) comporte les éléments de contact (49, 50, 65) sur différentes pièces d'éjecteur (44, 63) et est formé de manière à ce que, lors du déplacement de l'éjecteur (43) vers le bas, au moins après le contact d'au moins un premier élément de contact (49, 50) avec des pointes de pipette (89), au moins un deuxième élément de contact (65) soit en retard par rapport au premier élément de contact (49, 50) afin de détacher des tourillons (26), par pression, une ou plusieurs pointes de pipette (89) tout d'abord avec le premier élément de contact (49, 50), puis une ou plusieurs pointes de pipette (89) avec le deuxième élément de contact (65).
  2. Pipette multi-canal selon la revendication 1, dans laquelle les première et deuxième pièces d'éjecteur (44, 63) sont en forme de plaque, le premier élément de contact (49, 50) est au moins une première entretoise faisant saillie horizontalement du bord inférieur de la première pièce d'éjecteur (44) avec un ou plusieurs premiers trous de passage (51, 52), le deuxième élément de contact (65) est au moins une deuxième entretoise faisant saillie horizontalement du bord inférieur de la deuxième pièce d'éjecteur (63) avec un ou plusieurs deuxièmes trous de passage (66) et les tourillons (26) prennent à travers les premiers et deuxièmes trous de passage (51, 52, 66).
  3. Pipette multi-canal selon la revendication 1 ou 2, dans laquelle l'éjecteur (43) comporte une première pièce d'éjecteur (44) reliée au dispositif d'entraînement et une deuxième pièce d'éjecteur (63), la première pièce d'éjecteur (43) et la deuxième pièce d'éjecteur (63) étant guidées de manière mobile l'une par rapport à l'autre par des moyens de guidage (72, 73, 74, 75) dans le sens longitudinal des tourillons (26), la première pièce d'éjecteur (44) avec le premier élément de contact (49, 50) et la deuxième pièce d'éjecteur (63) avec le deuxième élément de contact (65) sont disposées au même niveau lorsque l'éjecteur (43) est à sa position initiale, et des premiers moyens d'entraînement (56) se trouvent sur la première pièce d'éjecteur (44) et des deuxièmes moyens d'entraînement (68) sur la deuxième pièce d'éjecteur (63), moyens d'entraînement espacés l'un de l'autre lorsque l'éjecteur (43) est à sa position initiale et pouvant être déplacés l'un contre l'autre lors du déplacement de l'éjecteur (43) vers le bas par déplacement de la première pièce d'éjecteur (44) et de la deuxième pièce d'éjecteur (63) l'une par rapport à l'autre le long des moyens de guidage (72, 73, 74, 75) afin de déplacer simultanément vers le bas la deuxième pièce d'éjecteur (63) avec la première pièce d'éjecteur (44) lors du contact des premiers avec les deuxièmes moyens d'entraînement (56, 68).
  4. Pipette multi-canal selon la revendication 3, dans laquelle les éléments de contact (49, 50, 65) sont des éléments de butée et prennent la position de butée lorsque l'éjecteur (43) est à sa position initiale.
  5. Pipette multi-canal selon la revendication 3 ou 4, dans laquelle la distance entre les premiers et deuxièmes moyens d'entraînement (56, 68) lorsque l'éjecteur (43) est à sa position initiale est 0,1 à 3 mm, de préférence 0,5 à 1,5 mm dans le sens vertical.
  6. Pipette multi-canal selon l'une quelconque des revendications 3 à 5, dans laquelle la première pièce d'éjecteur (44) comporte sur son bord inférieur un premier évidement (48) avec des premiers éléments de guidage (74, 75) sur deux bords latéraux, et dans laquelle la deuxième pièce d'éjecteur (63) est insérée dans le premier évidement (48) de la première pièce d'éjecteur (44) et est guidée de manière mobile sur des deuxièmes éléments de guidage (72, 73) sur deux bords latéraux sur les premiers éléments de guidage (74, 75) de la première pièce d'éjecteur (44) dans le sens longitudinal des tourillons (26).
  7. Pipette multi-canal selon la revendication 6, dans laquelle les premiers moyens d'entraînement (56) sont formés du bord supérieur de l'évidement (48) et les deuxièmes moyens d'entraînement (68) du bord supérieur de la deuxième pièce d'éjecteur (63).
  8. Pipette multi-canal selon la revendication 6 ou 7, dans laquelle la deuxième pièce d'éjecteur (63) est maintenue sur la première pièce d'éjecteur (44) par des moyens d'assemblage par encliquetage ou autre moyens de fixation (57, 70) qui présentent un jeu dans le sens longitudinal des tourillons (26).
  9. Pipette multi-canal selon la revendication 8, dans laquelle les moyens d'assemblage par encliquetage comportent un oeillet (57) sur la première pièce d'éjecteur (44) et un crochet d'encliquetage (70) sur la deuxième pièce d'éjecteur (63) prenant avec jeu dans le sens longitudinal des tourillons (26) dans l'oeillet (57) ou vice versa.
  10. Pipette multi-canal selon la revendication 1 ou 2, dans laquelle l'éjecteur (43.1, 43.2) comporte une première pièce d'éjecteur (44) rigide et une deuxième pièce d'éjecteur (63) au moins partiellement élastique souple, et la première pièce d'éjecteur (44) avec le premier élément de contact (49, 50) et la deuxième pièce d'éjecteur (63) avec le deuxième élément de contact (65) sont disposées au même niveau lorsque l'éjecteur (43.1, 43.2) est à sa position initiale, de manière à ce que lorsque les pièces d'éjecteur avec les éléments de contact touchent les pointes de pipette (89), d'abord la première pièce d'éjecteur (44) rigide détache par pression des pointes de pipette de tourillons et la deuxième pièce d'éjecteur (63) au moins partiellement élastique souple est bloquée et élastiquement pressée par des pointes de pipette (89) et, après une augmentation de la force de compression de la deuxième pièce d'éjecteur (63) au moins partiellement élastique souple à une valeur dépassant la force nécessaire pour éjecter la pointe de pipette (89) touchant le deuxième élément de contact (65), les pointes de pipette (89) touchant le deuxième élément de contact (65) sont éjectées.
  11. Pipette multi-canal selon l'une quelconque des revendications 1 à 10, dans laquelle les moyens de logement mobile (55, 76) de l'éjecteur (43) comportent au moins une fente de guidage (55) s'étendant dans le sens longitudinal des tourillons (26) dans l'éjecteur (43), et au moins un élément de guidage (76) lié de manière fixe au corps de base (5) et prenant dans la fente de guidage (55).
  12. Pipette multi-canal selon l'une quelconque des revendications 1 à 11, dans laquelle l'éjecteur (43) comporte en haut un élément d'actionnement (59) faisant saillie dans le sens longitudinal des tourillons (26).
  13. Pipette multi-canal selon l'une quelconque des revendications 1 à 12, dans laquelle l'éjecteur avec les premiers et deuxièmes éléments de butée (49, 50, 65) ou une autre pièce d'éjecteur aboute, lorsqu'il est à sa position initiale, à un élément de contre-butée (32) disposé de manière fixe sur le corps de base (5), élément de contre-butée qui empêche le déplacement de l'éjecteur plus loin vers le haut.
  14. Pipette multi-canal selon la revendication 13, dans laquelle l'élément de contre-butée (32) est une paroi inférieure du corps de base (5) avec des troisièmes trous de passage (31) à travers lesquels les tourillons (26) font saillie vers le bas et à laquelle les éléments de butée (49, 50, 65) aboutent en bas à la position initiale.
  15. Pipette multi-canal selon l'une quelconque des revendications 1 à 14, dans laquelle l'éjecteur (43) peut être déplacé vers le bas en s'opposant à l'effet de ressort d'un deuxième élément de suspension (82) prenant sur l'éjecteur (43) et sur le corps de base (5).
  16. Pipette multi-canal selon l'une quelconque des revendications 1 à 15, dans laquelle le nombre de tourillons (26) faisant saillie du premier élément de butée (49, 50) est égal au nombre de tourillons (26) faisant saillie du deuxième élément de butée (65).
EP12007916.5A 2012-11-23 2012-11-23 Pipette multi-canal Active EP2735369B1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
PL12007916T PL2735369T3 (pl) 2012-11-23 2012-11-23 Pipeta wielokanałowa
EP12007916.5A EP2735369B1 (fr) 2012-11-23 2012-11-23 Pipette multi-canal
US14/087,542 US9339811B2 (en) 2012-11-23 2013-11-22 Multi-channel pipette
CN201310757368.8A CN103990502B (zh) 2012-11-23 2013-11-22 多通道移液器
JP2013241905A JP6141177B2 (ja) 2012-11-23 2013-11-22 マルチチャンネルピペット

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP12007916.5A EP2735369B1 (fr) 2012-11-23 2012-11-23 Pipette multi-canal

Publications (2)

Publication Number Publication Date
EP2735369A1 EP2735369A1 (fr) 2014-05-28
EP2735369B1 true EP2735369B1 (fr) 2017-04-26

Family

ID=47257357

Family Applications (1)

Application Number Title Priority Date Filing Date
EP12007916.5A Active EP2735369B1 (fr) 2012-11-23 2012-11-23 Pipette multi-canal

Country Status (5)

Country Link
US (1) US9339811B2 (fr)
EP (1) EP2735369B1 (fr)
JP (1) JP6141177B2 (fr)
CN (1) CN103990502B (fr)
PL (1) PL2735369T3 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018002254A1 (fr) 2016-06-29 2018-01-04 Eppendorf Ag Tête de dosage, dispositif de dosage comprenant une tête de dosage et procédé de dosage au moyen d'une tête de dosage
US11130121B2 (en) 2016-06-21 2021-09-28 Eppendorf Ag Multichannel syringe for use with a metering device

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9044749B2 (en) * 2012-02-29 2015-06-02 Eppendorf Ag Pipette
US9815053B2 (en) * 2013-01-15 2017-11-14 Mettler-Toledo Rainin, LLC Liquid end assembly for a multichannel air displacement pipette
USD772426S1 (en) * 2014-01-13 2016-11-22 Gilson, Inc. Pipette system cartridge
CN104148126B (zh) * 2014-08-20 2016-06-22 南京中科神光科技有限公司 一种自适应退吸头装置
CN104148127B (zh) * 2014-08-20 2016-07-06 南京中科神光科技有限公司 一种双挂板自适应退吸头装置
JP6349039B2 (ja) * 2015-04-16 2018-06-27 インテグラ バイオサイエンシーズ アーゲー 手動ピペット用の容量調整機構
CH712010B1 (de) * 2016-01-11 2021-10-15 Integra Biosciences Ag Pipette mit Abwurfmechanismus für Pipettenspitzen.
FR3049877B1 (fr) * 2016-04-12 2018-04-13 Gilson Sas Pipette pour le prelevement d'une plage etendue de volumes de liquide
US10926454B2 (en) * 2016-05-20 2021-02-23 Sartorius Stedim Biotech Gmbh Dispensing device and system for biological products
DE102016111912A1 (de) * 2016-06-29 2018-01-04 Eppendorf Ag Dosierkopf, Dosiervorrichtung umfassend einen Dosierkopf und Verfahren zum Dosieren mittels eines Dosierkopfes
DE102016121810A1 (de) 2016-11-14 2018-05-17 Ika-Werke Gmbh & Co. Kg Handdosiervorrichtung
CH714486A1 (de) 2017-12-21 2019-06-28 Integra Biosciences Ag Probenverteilsystem und Verfahren zum Verteilen von Proben.
EP3560596A1 (fr) * 2018-04-23 2019-10-30 Eppendorf AG Pipette à utiliser avec une pointe de pipette et une famille de pipettes comprenant plusieurs pipettes avec des volumes nominaux différents
CN108745431A (zh) * 2018-04-29 2018-11-06 孝感鑫财智能科技有限公司 一种八通道式移液枪的枪头安装组件
EP3666379A1 (fr) 2018-12-14 2020-06-17 Eppendorf AG Tête de pipettage, dispositif de pipettage comprenant une tête de pipettage et procédé de pipettage à l'aide d'une tête de pipettage
CN109603945A (zh) * 2019-01-08 2019-04-12 安徽理工大学 一种多规格一体化移液器
USD924429S1 (en) 2019-02-21 2021-07-06 Brand Gmbh + Co Kg Handheld pipette
CN110270389B (zh) * 2019-07-22 2024-08-16 江苏集萃微纳自动化系统与装备技术研究所有限公司 一种移液机
EP3831486A1 (fr) * 2019-12-06 2021-06-09 Eppendorf AG Famille de pointes de pipette comprenant les pointes de pipette destinées à l'utilisation avec des pipettes d'une famille de pipettes et famille de pipettes comprenant les pipettes destinées à l'utilisation avec des pointes de pipette d'une famille de pointes de pipette
CN111389476B (zh) * 2020-01-04 2024-09-24 北京罗特思创科技有限公司 多通道柱塞泵及其使用方法
CN111811878B (zh) * 2020-06-12 2024-02-09 安徽云龙粮机有限公司 一种智能粮食扦样机的高稳定采样机构
USD978374S1 (en) * 2020-11-20 2023-02-14 Eppendorf Ag Pipette
EP4134166A1 (fr) * 2021-08-09 2023-02-15 Eppendorf SE Pipette à utiliser avec une pointe de pipette
CN113694980A (zh) * 2021-09-22 2021-11-26 于吉(南京)生物科技有限公司 一种多种培养液转移装置
CN114199631B (zh) * 2021-12-13 2022-08-12 杭州莱和生物技术有限公司 一种用于免疫层析监测信号的扩增方法
CN114931989B (zh) * 2022-05-17 2024-06-11 广州国睿科学仪器有限公司 一种多通道移液器
CN115090344A (zh) * 2022-07-30 2022-09-23 上海汇像信息技术有限公司 注液排枪
CN115254233B (zh) * 2022-08-31 2023-09-22 江西省烟草科学研究所 一种移液枪吸头快速装盒设备
USD1080912S1 (en) 2022-11-23 2025-06-24 Brand Gmbh + Co Kg Body for a handheld pipette device
US20240416335A1 (en) * 2023-06-09 2024-12-19 Life Technologies Holdings Pte Limited Multichannel pipette and electroporation system

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2701900B2 (ja) * 1988-12-20 1998-01-21 株式会社ニチリョー マルチピペット
US5983733A (en) * 1996-11-15 1999-11-16 Hamilton Company Manual pipette
CN1248495A (zh) * 1998-09-22 2000-03-29 陈国君 快速可调式移液器
JP2001305147A (ja) * 2000-04-19 2001-10-31 Hitachi Ltd 分注装置
JP2002122608A (ja) * 2000-10-13 2002-04-26 Tomiyuki Imai 分注装置
US6968749B2 (en) * 2002-11-21 2005-11-29 Arise Biotech Corporation Portable automated pipette
DE102004003433B4 (de) * 2004-01-21 2006-03-23 Eppendorf Ag Pipettiervorrichtung mit einer Abwurfeinrichtung für Pipettenspitzen
FI116612B (fi) * 2004-07-05 2006-01-13 Biohit Oyj Imulaite
DE102007006076B4 (de) * 2007-02-02 2008-10-30 Brand Gmbh + Co Kg Mehrkanal-Pipettiervorrichtung
DE102007010299B4 (de) * 2007-03-02 2009-01-29 Eppendorf Ag Handpipettiervorrichtung
FR2920675B1 (fr) * 2007-09-10 2010-12-03 Gilson Sas Systeme de pipetage multicanaux comprenant un porte-pistons a guidage ameliore
US20090071267A1 (en) * 2007-09-17 2009-03-19 Greg Mathus Pipette tip ejection mechanism
US7947234B2 (en) * 2007-10-17 2011-05-24 Rainin Instrument, Llc Liquid end assembly for a handheld multichannel pipette with adjustable nozzle spacing
US8029742B2 (en) * 2008-05-05 2011-10-04 Integra Biosciences Corp. Multi-channel pipettor with repositionable tips
WO2011140079A1 (fr) * 2010-05-03 2011-11-10 Integra Biosciences Corp. (Formerly Viaflo Corporation) Contrôle de mouvement imprévu pour pipette électronique multicanal à commande manuelle

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
None *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11130121B2 (en) 2016-06-21 2021-09-28 Eppendorf Ag Multichannel syringe for use with a metering device
WO2018002254A1 (fr) 2016-06-29 2018-01-04 Eppendorf Ag Tête de dosage, dispositif de dosage comprenant une tête de dosage et procédé de dosage au moyen d'une tête de dosage

Also Published As

Publication number Publication date
JP6141177B2 (ja) 2017-06-07
US9339811B2 (en) 2016-05-17
PL2735369T3 (pl) 2017-09-29
JP2014111255A (ja) 2014-06-19
CN103990502A (zh) 2014-08-20
CN103990502B (zh) 2016-10-26
EP2735369A1 (fr) 2014-05-28
US20140147349A1 (en) 2014-05-29

Similar Documents

Publication Publication Date Title
EP2735369B1 (fr) Pipette multi-canal
EP3478418B1 (fr) Tête de dosage, dispositif de dosage comprenant une tête de dosage et procédé de dosage au moyen d'une tête de dosage
EP2633913B1 (fr) Dispositif de pipetage et dispositif de pipetage multi-canal
EP1559480B1 (fr) Pipette avec mécanisme de déplacement fixé de façon détachable au mécanisme d'actionnement
DE102004003433B4 (de) Pipettiervorrichtung mit einer Abwurfeinrichtung für Pipettenspitzen
EP1759768B1 (fr) Pipette
EP3479129B1 (fr) Tête de dosage, dispositif de dosage comprenant une tête de dosage et procédé de dosage au moyen d'une tête de dosage
EP3793738A1 (fr) Tête de pipetage, dispositif de pipetage comprenant une tête de pipetage et procédé de pipetage au moyen d'une tête de pipetage
EP3164214B1 (fr) Pipette servant à faire fonctionner une seringue
EP1707269B1 (fr) Pipettage
EP2659978B1 (fr) Pipette avec systeme de blockage
EP3538275B1 (fr) Dispositif de dosage manuel
WO2014166635A1 (fr) Dispositif de pipette comportant une unite de microdosage
EP3680017B1 (fr) Pipette destinée à une utilisation avec une pointe de pipette
EP2626134B1 (fr) Pipette
EP2210667B1 (fr) Dispositif de dosage avec éjecteur de pointe
EP3260822B1 (fr) Seringue multivoie pour l'utilisation a l'aide d'un dispositif de dosage
EP2578317B1 (fr) Dispositif de dosage manuel
WO2025099184A1 (fr) Partie inférieure à canaux multiples destinée à un dispositif de transfert de fluide, et dispositif de transfert de fluide

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20140331

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

17Q First examination report despatched

Effective date: 20150312

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

INTG Intention to grant announced

Effective date: 20160506

GRAJ Information related to disapproval of communication of intention to grant by the applicant or resumption of examination proceedings by the epo deleted

Free format text: ORIGINAL CODE: EPIDOSDIGR1

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

INTC Intention to grant announced (deleted)
INTG Intention to grant announced

Effective date: 20160812

GRAJ Information related to disapproval of communication of intention to grant by the applicant or resumption of examination proceedings by the epo deleted

Free format text: ORIGINAL CODE: EPIDOSDIGR1

INTC Intention to grant announced (deleted)
GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: GRANT OF PATENT IS INTENDED

INTG Intention to grant announced

Effective date: 20161125

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE PATENT HAS BEEN GRANTED

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

Free format text: NOT ENGLISH

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: AT

Ref legal event code: REF

Ref document number: 887397

Country of ref document: AT

Kind code of ref document: T

Effective date: 20170515

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

Free format text: LANGUAGE OF EP DOCUMENT: GERMAN

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 502012010142

Country of ref document: DE

REG Reference to a national code

Ref country code: CH

Ref legal event code: NV

Representative=s name: ISLER AND PEDRAZZINI AG, CH

REG Reference to a national code

Ref country code: NL

Ref legal event code: MP

Effective date: 20170426

REG Reference to a national code

Ref country code: LT

Ref legal event code: MG4D

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: NL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170727

Ref country code: LT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

Ref country code: ES

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

Ref country code: NO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170726

Ref country code: HR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 6

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LV

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170826

Ref country code: BG

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170726

Ref country code: SE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

Ref country code: RS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 502012010142

Country of ref document: DE

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

Ref country code: EE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

Ref country code: DK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SM

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

Ref country code: IT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

26N No opposition filed

Effective date: 20180129

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MC

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LU

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20171123

REG Reference to a national code

Ref country code: BE

Ref legal event code: MM

Effective date: 20171130

REG Reference to a national code

Ref country code: IE

Ref legal event code: MM4A

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20171123

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: BE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20171130

REG Reference to a national code

Ref country code: AT

Ref legal event code: MM01

Ref document number: 887397

Country of ref document: AT

Kind code of ref document: T

Effective date: 20171123

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: AT

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20171123

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: HU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT; INVALID AB INITIO

Effective date: 20121123

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CY

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170426

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: TR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: AL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170426

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 11

REG Reference to a national code

Ref country code: DE

Ref legal event code: R081

Ref document number: 502012010142

Country of ref document: DE

Owner name: EPPENDORF SE, DE

Free format text: FORMER OWNER: EPPENDORF AG, 22339 HAMBURG, DE

P01 Opt-out of the competence of the unified patent court (upc) registered

Effective date: 20230527

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: DE

Payment date: 20241121

Year of fee payment: 13

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: FI

Payment date: 20241122

Year of fee payment: 13

Ref country code: PL

Payment date: 20241114

Year of fee payment: 13

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20241120

Year of fee payment: 13

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: FR

Payment date: 20241128

Year of fee payment: 13

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: CH

Payment date: 20241201

Year of fee payment: 13