DK3517607T3 - Hidtil ukendt aspartokinase-mutant og fremgangsmåde til fremstilling af L-aminosyre under anvendelse af samme - Google Patents
Hidtil ukendt aspartokinase-mutant og fremgangsmåde til fremstilling af L-aminosyre under anvendelse af samme Download PDFInfo
- Publication number
- DK3517607T3 DK3517607T3 DK18824928.8T DK18824928T DK3517607T3 DK 3517607 T3 DK3517607 T3 DK 3517607T3 DK 18824928 T DK18824928 T DK 18824928T DK 3517607 T3 DK3517607 T3 DK 3517607T3
- Authority
- DK
- Denmark
- Prior art keywords
- producing
- amino acid
- same
- novel
- aspartokinase mutant
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1217—Phosphotransferases with a carboxyl group as acceptor (2.7.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/001—Amines; Imines
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/12—Methionine; Cysteine; Cystine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/02—Phosphotransferases with a carboxy group as acceptor (2.7.2)
- C12Y207/02004—Aspartate kinase (2.7.2.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/02—Phosphotransferases with a carboxy group as acceptor (2.7.2)
- C12Y207/02003—Phosphoglycerate kinase (2.7.2.3)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020170083437A KR102011994B1 (ko) | 2017-06-30 | 2017-06-30 | 신규한 아스파토키나제 변이체 및 이를 이용한 l-아미노산의 제조방법 |
| PCT/KR2018/007407 WO2019004778A2 (ko) | 2017-06-30 | 2018-06-29 | 신규한 아스파토키나제 변이체 및 이를 이용한 l-아미노산의 제조방법 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3517607T3 true DK3517607T3 (da) | 2021-07-26 |
Family
ID=64742506
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK18824928.8T DK3517607T3 (da) | 2017-06-30 | 2018-06-29 | Hidtil ukendt aspartokinase-mutant og fremgangsmåde til fremstilling af L-aminosyre under anvendelse af samme |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US10662450B2 (da) |
| EP (1) | EP3517607B1 (da) |
| JP (1) | JP6759484B1 (da) |
| KR (1) | KR102011994B1 (da) |
| CN (1) | CN110168083B (da) |
| AR (1) | AR112431A1 (da) |
| AU (1) | AU2018293281B2 (da) |
| BR (1) | BR112019018036B8 (da) |
| DK (1) | DK3517607T3 (da) |
| ES (1) | ES2884800T3 (da) |
| HU (1) | HUE054725T2 (da) |
| MX (1) | MX388542B (da) |
| MY (1) | MY195408A (da) |
| PH (1) | PH12019502673A1 (da) |
| PL (1) | PL3517607T3 (da) |
| RU (1) | RU2730025C1 (da) |
| SG (1) | SG11201912130RA (da) |
| TW (1) | TWI718385B (da) |
| WO (1) | WO2019004778A2 (da) |
| ZA (1) | ZA201907864B (da) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3931853B1 (en) | 2019-08-16 | 2024-06-19 | Protochips, Inc. | Automated application of drift correction to sample studied under electron microscope |
| KR102153534B1 (ko) | 2019-09-02 | 2020-09-09 | 씨제이제일제당 주식회사 | 신규한 프로모터 및 이를 이용한 아미노산 생산 방법 |
| KR102183209B1 (ko) | 2019-09-09 | 2020-11-26 | 씨제이제일제당 주식회사 | L-쓰레오닌 배출 단백질의 변이체 및 이를 이용한 l-쓰레오닌 생산 방법 |
| KR102182497B1 (ko) | 2019-12-20 | 2020-11-24 | 씨제이제일제당 주식회사 | 내막 단백질의 변이체 및 이를 이용한 목적 산물 생산 방법 |
| KR102207867B1 (ko) | 2020-01-21 | 2021-01-26 | 씨제이제일제당 주식회사 | Nadp 의존적 글리세르알데하이드-3-포스페이트 디하이드로지나제를 포함하는 미생물을 이용하여 ｌ-아미노산을 생산하는 방법 |
| KR102285951B1 (ko) * | 2020-01-30 | 2021-08-04 | 씨제이제일제당 주식회사 | 시트레이트 신타아제의 활성이 약화된 신규한 변이형 폴리펩티드 및 이를 이용한 l-아미노산 생산 방법 |
| CA3199126A1 (en) | 2020-12-11 | 2022-06-16 | Byoung Hoon Yoon | Mutant atp-dependent protease, and method for producing l-amino acid using same |
| KR102470602B1 (ko) | 2020-12-11 | 2022-11-25 | 씨제이제일제당 주식회사 | 신규한 분지 연쇄 아미노산 아미노트렌스퍼라아제 변이체 및 이를 이용한 이소류신 생산 방법 |
| KR102464883B1 (ko) | 2020-12-11 | 2022-11-09 | 씨제이제일제당 주식회사 | 신규한 감마-아미노부티르산 퍼미에이즈 변이체 및 이를 이용한 이소류신 생산 방법 |
| EP4060034B1 (en) * | 2021-01-25 | 2024-10-02 | CJ Cheiljedang Corporation | Novel hydrolase variant, and method for producing l-tryptophan using same |
| CN114450400B (zh) * | 2021-01-25 | 2022-10-04 | Cj第一制糖株式会社 | 新型铜转运p型atp酶a变体及使用其生产l-色氨酸的方法 |
| KR102377745B1 (ko) | 2021-05-12 | 2022-03-23 | 씨제이제일제당 주식회사 | 신규 프로모터 및 이의 용도 |
| KR102685904B1 (ko) | 2021-05-20 | 2024-07-19 | 씨제이제일제당 주식회사 | 신규 프로모터 및 이의 용도 |
| MY209952A (en) | 2021-05-20 | 2025-08-15 | Cj Cheiljedang Corp | Novel promoter and use thereof |
| KR20230139597A (ko) | 2022-03-28 | 2023-10-05 | 씨제이제일제당 (주) | O-아세틸 호모세린 생산 미생물 및 이를 이용한 o-아세틸 호모세린 또는 l-메치오닌 생산 방법 |
| KR20230139598A (ko) | 2022-03-28 | 2023-10-05 | 씨제이제일제당 (주) | O-아세틸 호모세린 생산 미생물 및 이를 이용한 o-아세틸 호모세린 또는 l-메치오닌 생산 방법 |
| CN115725537B (zh) * | 2022-07-11 | 2025-09-12 | 天津国家合成生物技术创新中心有限公司 | 天冬氨酸激酶突变体及其在生产l-高丝氨酸中的应用 |
| KR20240143216A (ko) | 2023-03-23 | 2024-10-02 | 씨제이제일제당 (주) | 변이형 트레오닌/호모세린 배출 단백질 및 이를 이용한 l-아미노산 생산 방법 |
| KR20240152455A (ko) | 2023-04-12 | 2024-10-22 | 씨제이제일제당 (주) | 피루브산 탈수소효소 복합체 디히드로리포일리신-잔기 아세틸트랜스퍼라제의 활성이 증가된 미생물 및 이를 이용한 l-아미노산 생산 방법 |
| KR20240152456A (ko) | 2023-04-12 | 2024-10-22 | 씨제이제일제당 (주) | 피루브산 탈수소효소 복합체 서브유닛 e1의 활성이 증가된 미생물 및 이를 이용한 o-아세틸호모세린 또는 그의 유도체의 생산 방법 |
| WO2025005633A1 (ko) | 2023-06-28 | 2025-01-02 | 씨제이제일제당 (주) | 쓰레오닌 배출 단백질 rhtc를 발현하는 미생물 및 이의 l-이소류신 생산 용도 |
| CN118773161B (zh) * | 2024-06-13 | 2025-02-14 | 华南师范大学 | 丝氨酸激酶和硫裂解酶的突变酶及其发酵生产方法以及在生产含羞草素中的应用 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3473042B2 (ja) * | 1992-04-28 | 2003-12-02 | 味の素株式会社 | 変異型アスパルトキナーゼ遺伝子 |
| ATE203769T1 (de) * | 1992-11-10 | 2001-08-15 | Ajinomoto Kk | Dna, die aspartokinase iii-mutanten kodiert und ihre verwendung zur herstellung von l-threonin durch fermentation |
| JPH07155184A (ja) * | 1993-12-08 | 1995-06-20 | Ajinomoto Co Inc | 発酵法によるｌ−リジンの製造法 |
| JP4075087B2 (ja) * | 1996-12-05 | 2008-04-16 | 味の素株式会社 | Ｌ−リジンの製造法 |
| AU2000230762A1 (en) * | 2000-01-21 | 2001-07-31 | Ajinomoto Co. Inc. | Process for producing l-lysine |
| RU2224793C1 (ru) * | 2000-01-21 | 2004-02-27 | Адзиномото Ко., Инк. | Способ получения l-лизина |
| WO2003040373A2 (en) * | 2001-08-06 | 2003-05-15 | Degussa Ag | Production of l-lysine by genetically modified corynebacterium glutamicum strains |
| JP2007068437A (ja) * | 2005-09-05 | 2007-03-22 | Chisso Corp | 変異型アスパルトキナーゼとその利用方法 |
| KR100838038B1 (ko) | 2006-12-29 | 2008-06-12 | 씨제이제일제당 (주) | Ｌ-라이신 생산능이 향상된 코리네박테리움 속 미생물 및그를 이용한 ｌ-라이신 생산 방법 |
| US9005952B2 (en) * | 2008-04-04 | 2015-04-14 | Cj Cheiljedang Corporation | Microorganism producing L-methionine precursor and the method of producing L-methionine precursor using the microorganism |
| CN102753692A (zh) * | 2010-06-15 | 2012-10-24 | 白光产业株式会社 | 使用微生物生产天冬氨酸族氨基酸的方法 |
-
2017
- 2017-06-30 KR KR1020170083437A patent/KR102011994B1/ko active Active
-
2018
- 2018-06-29 PL PL18824928T patent/PL3517607T3/pl unknown
- 2018-06-29 DK DK18824928.8T patent/DK3517607T3/da active
- 2018-06-29 MX MX2019014609A patent/MX388542B/es unknown
- 2018-06-29 CN CN201880005277.XA patent/CN110168083B/zh active Active
- 2018-06-29 RU RU2019113249A patent/RU2730025C1/ru active
- 2018-06-29 AU AU2018293281A patent/AU2018293281B2/en active Active
- 2018-06-29 EP EP18824928.8A patent/EP3517607B1/en active Active
- 2018-06-29 HU HUE18824928A patent/HUE054725T2/hu unknown
- 2018-06-29 US US16/344,189 patent/US10662450B2/en active Active
- 2018-06-29 TW TW107122554A patent/TWI718385B/zh active
- 2018-06-29 SG SG11201912130RA patent/SG11201912130RA/en unknown
- 2018-06-29 WO PCT/KR2018/007407 patent/WO2019004778A2/ko not_active Ceased
- 2018-06-29 ES ES18824928T patent/ES2884800T3/es active Active
- 2018-06-29 MY MYPI2019006841A patent/MY195408A/en unknown
- 2018-06-29 BR BR112019018036A patent/BR112019018036B8/pt active IP Right Grant
- 2018-06-29 AR ARP180101815 patent/AR112431A1/es unknown
-
2019
- 2019-11-26 PH PH12019502673A patent/PH12019502673A1/en unknown
- 2019-11-27 ZA ZA2019/07864A patent/ZA201907864B/en unknown
-
2020
- 2020-07-14 JP JP2020120874A patent/JP6759484B1/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| BR112019018036B8 (pt) | 2022-03-29 |
| EP3517607A2 (en) | 2019-07-31 |
| MY195408A (en) | 2023-01-19 |
| AU2018293281A1 (en) | 2019-12-12 |
| PH12019502673A1 (en) | 2020-06-08 |
| AU2018293281B2 (en) | 2022-02-03 |
| MX388542B (es) | 2025-03-20 |
| BR112019018036B1 (pt) | 2022-03-22 |
| TW201907002A (zh) | 2019-02-16 |
| ZA201907864B (en) | 2020-08-26 |
| EP3517607A4 (en) | 2020-05-13 |
| US10662450B2 (en) | 2020-05-26 |
| TWI718385B (zh) | 2021-02-11 |
| BR112019018036A2 (pt) | 2020-04-07 |
| AR112431A1 (es) | 2019-10-30 |
| MX2019014609A (es) | 2020-02-07 |
| SG11201912130RA (en) | 2020-01-30 |
| HUE054725T2 (hu) | 2021-09-28 |
| US20200131545A1 (en) | 2020-04-30 |
| CN110168083B (zh) | 2020-10-27 |
| WO2019004778A2 (ko) | 2019-01-03 |
| CN110168083A (zh) | 2019-08-23 |
| JP6759484B1 (ja) | 2020-09-23 |
| PL3517607T3 (pl) | 2021-12-20 |
| EP3517607B1 (en) | 2021-05-05 |
| KR102011994B1 (ko) | 2019-08-20 |
| WO2019004778A3 (ko) | 2019-03-28 |
| JP2020202834A (ja) | 2020-12-24 |
| KR20190003019A (ko) | 2019-01-09 |
| ES2884800T3 (es) | 2021-12-13 |
| RU2730025C1 (ru) | 2020-08-14 |
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