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DK3154995T3 - Separation of 2'-o-fucosyllactose from fermentation broth - Google Patents

Separation of 2'-o-fucosyllactose from fermentation broth Download PDF

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Publication number
DK3154995T3
DK3154995T3 DK15806229.9T DK15806229T DK3154995T3 DK 3154995 T3 DK3154995 T3 DK 3154995T3 DK 15806229 T DK15806229 T DK 15806229T DK 3154995 T3 DK3154995 T3 DK 3154995T3
Authority
DK
Denmark
Prior art keywords
fucosyllactose
separation
fermentation broth
broth
fermentation
Prior art date
Application number
DK15806229.9T
Other languages
English (en)
Inventor
Martin Matwiejuk
Pierre Chassagne
Gyula Dekany
Györgyi Osztrovszky
Ferenc Horváth
Original Assignee
Glycom As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glycom As filed Critical Glycom As
Application granted granted Critical
Publication of DK3154995T3 publication Critical patent/DK3154995T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D9/00—Crystallisation
    • B01D9/0036—Crystallisation on to a bed of product crystals; Seeding
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D9/00—Crystallisation
    • B01D9/005—Selection of auxiliary, e.g. for control of crystallisation nuclei, of crystal growth, of adherence to walls; Arrangements for introduction thereof
    • B01D9/0054—Use of anti-solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK15806229.9T 2014-06-11 2015-06-11 Separation of 2'-o-fucosyllactose from fermentation broth DK3154995T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA201470348 2014-06-11
PCT/DK2015/050158 WO2015188834A1 (en) 2014-06-11 2015-06-11 Separation of 2'-o-fucosyllactose from fermentation broth

Publications (1)

Publication Number Publication Date
DK3154995T3 true DK3154995T3 (da) 2023-11-20

Family

ID=54832929

Family Applications (4)

Application Number Title Priority Date Filing Date
DK15806229.9T DK3154995T3 (da) 2014-06-11 2015-06-11 Separation of 2'-o-fucosyllactose from fermentation broth
DKBA201900111U DK201900111U1 (da) 2014-06-11 2019-12-19 Separation of 2'-o-fucosyllactose from fermentation broth
DKBA202000086U DK202000086Y4 (da) 2014-06-11 2020-09-11 Separation af 2’-FL fra en fermentationskulturopløsning
DKBA202100114U DK202100114Y4 (da) 2014-06-11 2021-12-17 Separation af 2’-FL fra en fermentationskulturopløsning

Family Applications After (3)

Application Number Title Priority Date Filing Date
DKBA201900111U DK201900111U1 (da) 2014-06-11 2019-12-19 Separation of 2'-o-fucosyllactose from fermentation broth
DKBA202000086U DK202000086Y4 (da) 2014-06-11 2020-09-11 Separation af 2’-FL fra en fermentationskulturopløsning
DKBA202100114U DK202100114Y4 (da) 2014-06-11 2021-12-17 Separation af 2’-FL fra en fermentationskulturopløsning

Country Status (5)

Country Link
EP (1) EP3154995B1 (da)
DE (1) DE202015009782U1 (da)
DK (4) DK3154995T3 (da)
ES (1) ES2968677T3 (da)
WO (1) WO2015188834A1 (da)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9902984B2 (en) * 2013-09-06 2018-02-27 Glycom A/S Fermentative production of oligosaccharides
EP2896628B1 (en) 2014-01-20 2018-09-19 Jennewein Biotechnologie GmbH Process for efficient purification of neutral human milk oligosaccharides (HMOs) from microbial fermentation
DE202015009775U1 (de) 2014-12-16 2020-02-05 Glycom A/S Abscheidung von 2'-FL aus einer Fermentationsbrühe
US10899782B2 (en) 2016-03-07 2021-01-26 Glycom A/S Separation of oligosaccharides from fermentation broth
EP3445770B1 (en) * 2016-04-19 2025-11-26 Glycom A/S Separation of oligosaccharides from fermentation broth
WO2018077368A1 (en) 2016-10-31 2018-05-03 Glycom A/S Method for removing residual organic solvent from a crystalline oligosaccharide
CN110621685B (zh) * 2017-03-06 2024-07-09 因比奥斯公司 用于使2’-岩藻糖基乳糖结晶的方法和相关组合物
WO2018164937A1 (en) 2017-03-06 2018-09-13 Dupont Nutrition Biosciences Aps Process for crystallizing 2'-fucosyllactose and related compositions
CN110914284B (zh) 2017-07-12 2025-01-07 格礼卡姆股份公司 包含中性单糖或寡糖和酸性非碳水化合物组分的无定形混合物
EP3728596A4 (en) 2017-12-21 2021-08-25 Glycom A/S CONSTRUCTION OF NUCLEIC ACID ALLOWING THE EXPRESSION OF A GENE IN VITRO AND IN VIVO
WO2020079146A1 (en) 2018-10-18 2020-04-23 Basf Se Crystalline form ii of 2'-o-fucosyllactose, process for its preparation, nutritional, cosmetic or pharmaceutical formulation containing the same
JP2023511522A (ja) 2020-01-23 2023-03-20 グリコム・アクティーゼルスカブ Ｈｍｏの産生
CN114981291B (zh) 2020-01-23 2024-12-06 格礼卡姆股份公司 Hmo产生
US20230072639A1 (en) 2020-01-23 2023-03-09 Glycom A/S New major facilitator superfamily (mfs) protein (bad) in hmo production
BR112022014414A2 (pt) 2020-01-23 2022-09-13 Glycom As Produção de hmo
KR20220154158A (ko) * 2020-03-12 2022-11-21 글리콤 에이/에스 2'-fl의 결정화
DK180952B1 (en) 2020-12-22 2022-08-10 Glycom As A dfl-producing strain
EP4281564A1 (en) 2021-01-22 2023-11-29 Glycom A/S New major facilitator superfamily (mfs) protein (fred) in production of sialylated hmos
CN112920234B (zh) * 2021-01-27 2022-03-29 南开大学 一种2′-岩藻糖基乳糖的富集纯化方法
DK181497B1 (en) 2021-05-17 2024-03-12 Dsm Ip Assets Bv ENHANCING FORMATION OF THE HMOS LNT AND/OR LNnT BY MODIFYING LACTOSE IMPORT IN THE CELL
EP4341408A1 (en) 2021-05-17 2024-03-27 DSM IP Assets B.V. Methods of producing hmo blend profiles with lnfp-i and lnt as the predominant compounds
WO2022243314A2 (en) 2021-05-17 2022-11-24 Dsm Ip Assets B.V. Methods of producing hmo blend profiles with lnfp-i and 2'-fl as the predominant compounds
DK181242B1 (en) 2021-05-17 2023-05-30 Dsm Ip Assets Bv GENETICALLY ENGINEERED CELLS COMPRISING A RECOMBINANT NUCLEIC ACID SEQUNCE ENCODING AN α-1,2-FUCOSYLTRANSFERASE CAPABLE OF PRODUCING LNFP-I, NUCLEIC ACID SEQUENCES ENCODING SAME AND METHODS FOR USE OF SAME
DK181310B1 (en) 2021-05-17 2023-08-08 Dsm Ip Assets Bv Cell factories for lnt-ii production
WO2022243310A1 (en) 2021-05-17 2022-11-24 Dsm Ip Assets B.V. Novel technology to enable sucrose utilization in strains for biosyntetic production
EP4355464A1 (en) 2021-06-15 2024-04-24 DSM IP Assets B.V. Separation of human milk oligosaccharides from a fermentation broth
BE1029435B1 (nl) 2021-06-15 2023-07-31 Dsm Ip Assets Bv Scheiding van moedermelkoligosachariden uit een fermentatiebouillon
BE1029436B1 (nl) 2021-06-15 2023-07-31 Dsm Ip Assets Bv Scheiding van moedermelkoligosachariden uit een fermentatiebouillon
US20240287116A1 (en) 2021-06-15 2024-08-29 Dsm Ip Assets B.V. Separation of human milk oligosaccharides from a fermentation broth
DK181991B1 (en) 2021-11-11 2025-05-15 Dsm Ip Assets Bv Combined fermentation process for producing one or more human milk oligosaccharide(s) (hmo(s)) in escherichia coli
US20250382649A1 (en) 2021-12-01 2025-12-18 Dsm Ip Assets B.V. Cells with tri-, tetra- or pentasaccharide importers useful in oligosaccharide production
DK182075B1 (en) 2022-06-13 2025-07-02 Dsm Ip Assets Bv Genetically engineered cells comprising sigma factor modifications for production of heterologous products and methods for use of same
DK181765B1 (en) 2022-07-15 2024-12-04 Dsm Ip Assets Bv Cells expressing new fucosyltransferases for in vivo synthesis of lnfp-iii, and methods and uses of same
EP4555077A1 (en) 2022-07-15 2025-05-21 DSM IP Assets B.V. New fucosyltransferases for in vivo synthesis of complex fucosylated human milk oligosaccharides
KR20250054803A (ko) 2022-08-25 2025-04-23 디에스엠 아이피 어셋츠 비.브이. 복합 hmo 생산을 위한 하이브리드 방법
EP4623089A1 (en) 2022-11-25 2025-10-01 DSM IP Assets B.V. Two-strain system for producing oligosaccharides
DK202201117A1 (en) 2022-12-09 2024-08-08 Dsm Ip Assets Bv Genetically modified udp-n-acetylgalactosamine producing cells
DK181996B1 (en) 2022-12-22 2025-05-16 Dsm Ip Assets Bv Cells expressing a new fucosyltransferase osc-1 for production of 3fl, and uses and methods for producing 3-fl.
DK202201202A1 (en) 2022-12-22 2024-08-21 Dsm Ip Assets Bv New fucosyltransferases for in vivo synthesis of complex fucosylated human milk oligosaccharides mixtures comprising lndfh-iii
CN120677242A (zh) 2023-02-24 2025-09-19 帝斯曼知识产权资产管理有限公司 用于体内合成人乳寡糖的产物特异性转运蛋白
DK202330269A1 (en) 2023-10-17 2025-06-16 Dsm Ip Assets Bv New fucosyltransferases for in vivo synthesis of complex fucosylated human milk oligosaccharides mixtures comprising lnfp-vi or lnfp-v
WO2025190861A1 (en) 2024-03-11 2025-09-18 Dsm Ip Assets B.V. Fucosyltransferase mutants with low dfl formation

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2796082B1 (fr) 1999-07-07 2003-06-27 Centre Nat Rech Scient Procede de production d'oligosaccharides
IT1392456B1 (it) 2008-12-18 2012-03-09 Inalco Spa Processo per la sintesi di di- o oligosaccaridi l-fucosilati e loro nuovi intermedi 2,3,4-tribenzil-fucosil-derivati.
RU2584599C2 (ru) 2008-12-19 2016-05-20 Дженневейн Биотехнологие Гмбх Синтез фукозилированных соединений
EP2576578B1 (en) 2010-06-01 2018-09-19 Glycom A/S Polymorphs of 2'-o-fucosyllactose and producing thereof
MX386398B (es) 2010-07-12 2025-03-18 Inbiose Nv Organismos metabólicamente diseñados para la producción de bioproductos de valor agregado.
DK2479263T3 (da) 2011-01-20 2014-02-03 Jennewein Biotechnologie Gmbh Nye fucosyltransferaser og deres anvendelser
US9453230B2 (en) 2011-02-16 2016-09-27 Glycosyn LLC Biosynthesis of human milk oligosaccharides in engineered bacteria
ITFI20120143A1 (it) 2012-07-12 2014-01-13 Inalco S A S Di Giovanni Cipollett I & C Polimorfi idrati e anidri del 2'-o-fucosillattosio e loro metodi di produzione.
WO2014069625A1 (ja) * 2012-11-01 2014-05-08 協和発酵バイオ株式会社 オリゴ糖の結晶およびその製造方法
WO2014086373A1 (en) * 2012-12-07 2014-06-12 Glycom A/S Crystallisation of human milk oligosaccharides (hmo)

Also Published As

Publication number Publication date
EP3154995A4 (en) 2017-12-13
DK202000086U1 (en) 2020-12-07
DK202100114U1 (en) 2021-12-21
EP3154995A1 (en) 2017-04-19
ES2968677T3 (es) 2024-05-13
DK202100114Y4 (da) 2022-06-02
EP3154995B1 (en) 2023-11-01
WO2015188834A1 (en) 2015-12-17
DK201900111U1 (da) 2020-01-06
DK202000086Y4 (da) 2023-02-02
DE202015009782U1 (de) 2020-02-10

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