DK1885880T3 - Amplifikation og kloning af enkelte DNA-molekyler ved anvendelse af rolling circle-amplifikation - Google Patents
Amplifikation og kloning af enkelte DNA-molekyler ved anvendelse af rolling circle-amplifikationInfo
- Publication number
- DK1885880T3 DK1885880T3 DK06751837.3T DK06751837T DK1885880T3 DK 1885880 T3 DK1885880 T3 DK 1885880T3 DK 06751837 T DK06751837 T DK 06751837T DK 1885880 T3 DK1885880 T3 DK 1885880T3
- Authority
- DK
- Denmark
- Prior art keywords
- amplification
- rolling circle
- cloning
- dna molecules
- single dna
- Prior art date
Links
- 230000003321 amplification Effects 0.000 title abstract 5
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 5
- 238000005096 rolling process Methods 0.000 title abstract 4
- 238000010367 cloning Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 abstract 4
- 108020004414 DNA Proteins 0.000 abstract 2
- 108020004638 Circular DNA Proteins 0.000 abstract 1
- 102000053602 DNA Human genes 0.000 abstract 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 238000006073 displacement reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cell Biology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Analytical Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US67585005P | 2005-04-29 | 2005-04-29 | |
| US72207005P | 2005-09-30 | 2005-09-30 | |
| US72530005P | 2005-10-12 | 2005-10-12 | |
| PCT/US2006/016349 WO2006119066A2 (en) | 2005-04-29 | 2006-05-01 | Amplification and cloning of single dna molecules using rolling circle amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1885880T3 true DK1885880T3 (da) | 2010-11-08 |
Family
ID=36962781
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK06751837.3T DK1885880T3 (da) | 2005-04-29 | 2006-05-01 | Amplifikation og kloning af enkelte DNA-molekyler ved anvendelse af rolling circle-amplifikation |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US8497069B2 (da) |
| EP (1) | EP1885880B1 (da) |
| CN (1) | CN101213311B (da) |
| AT (1) | ATE474937T1 (da) |
| AU (1) | AU2006242387B2 (da) |
| BR (1) | BRPI0607661A2 (da) |
| CA (1) | CA2607532C (da) |
| DE (1) | DE602006015633D1 (da) |
| DK (1) | DK1885880T3 (da) |
| IL (1) | IL187002A (da) |
| WO (1) | WO2006119066A2 (da) |
Families Citing this family (69)
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| WO2008151023A2 (en) | 2007-06-01 | 2008-12-11 | Ibis Biosciences, Inc. | Methods and compositions for multiple displacement amplification of nucleic acids |
| KR101414713B1 (ko) | 2007-10-11 | 2014-07-03 | 삼성전자주식회사 | 리가제 및 엔도뉴클레아제의 존재하에서 롤링서클 증폭에의하여 표적 핵산을 증폭하는 방법 |
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| MY180599A (en) | 2008-02-23 | 2020-12-03 | Weifu Lee James | Designer organisms for photobiological butanol production from carbon dioxide and water |
| US8986963B2 (en) * | 2008-02-23 | 2015-03-24 | James Weifu Lee | Designer calvin-cycle-channeled production of butanol and related higher alcohols |
| US9593366B2 (en) * | 2008-10-30 | 2017-03-14 | Qiagen Gaithersburg, Inc. | Individually synthesized primers to be used in whole genome amplification |
| GB0901593D0 (en) | 2009-01-30 | 2009-03-11 | Touchlight Genetics Ltd | Production of closed linear DNA |
| CN104722342B (zh) | 2009-03-24 | 2017-01-11 | 芝加哥大学 | 滑动式芯片装置和方法 |
| US10196700B2 (en) | 2009-03-24 | 2019-02-05 | University Of Chicago | Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes |
| US9464319B2 (en) | 2009-03-24 | 2016-10-11 | California Institute Of Technology | Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes |
| US9447461B2 (en) | 2009-03-24 | 2016-09-20 | California Institute Of Technology | Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes |
| US9890408B2 (en) | 2009-10-15 | 2018-02-13 | Ibis Biosciences, Inc. | Multiple displacement amplification |
| US20110195457A1 (en) * | 2010-02-09 | 2011-08-11 | General Electric Company | Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof |
| GB201013153D0 (en) | 2010-08-04 | 2010-09-22 | Touchlight Genetics Ltd | Primer for production of closed linear DNA |
| AU2012304328B2 (en) | 2011-09-09 | 2017-07-20 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for obtaining a sequence |
| US9353393B2 (en) | 2012-02-15 | 2016-05-31 | General Electric Company | Methods and kits for reducing non-specific nucleic acid amplification |
| EP2959018A4 (en) * | 2013-02-22 | 2016-10-26 | Broad Inst Inc | METHOD OF RISK ASSESSMENT OF ATOPIC DERMATITIS IN DOGS |
| EP2971123B1 (en) | 2013-03-15 | 2021-05-05 | Labrador Diagnostics LLC | Nucleic acid amplification |
| US20140302504A1 (en) | 2013-03-15 | 2014-10-09 | Theranos, Inc. | Nucleic Acid Amplification |
| US9416387B2 (en) * | 2013-03-15 | 2016-08-16 | Theranos, Inc. | Nucleic acid amplification |
| US10450595B2 (en) | 2013-03-15 | 2019-10-22 | Theranos Ip Company, Llc | Nucleic acid amplification |
| CN103276074B (zh) * | 2013-05-21 | 2015-08-19 | 昆山彭济凯丰生物科技有限公司 | 一种合成短单链脱氧核糖核苷酸探针的方法 |
| TWI707038B (zh) | 2013-08-05 | 2020-10-11 | 美商扭轉生物科技有限公司 | 重新合成之基因庫 |
| EP3042208A4 (en) | 2013-09-06 | 2017-04-19 | Theranos, Inc. | Systems and methods for detecting infectious diseases |
| CN104695027B (zh) | 2013-12-06 | 2017-10-20 | 中国科学院北京基因组研究所 | 测序文库及其制备和应用 |
| WO2015157747A1 (en) * | 2014-04-11 | 2015-10-15 | Redvault Biosciences Lp | Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications |
| PT3152331T (pt) * | 2014-06-06 | 2019-11-05 | Univ Cornell | Método para identificação e enumeração de alterações de sequência de ácidos nucleicos, expressão, cópia ou metilação de adn, utilizando reações de nuclease, ligase, polimerase e sequenciação combinadas |
| WO2016022557A1 (en) * | 2014-08-05 | 2016-02-11 | Twist Bioscience Corporation | Cell free cloning of nucleic acids |
| GB201415789D0 (en) * | 2014-09-05 | 2014-10-22 | Touchlight Genetics Ltd | Synthesis of DNA |
| CN104328111A (zh) * | 2014-10-29 | 2015-02-04 | 北京大学 | 一种用于测序的高通量扩增质粒的方法 |
| CA2975855C (en) | 2015-02-04 | 2025-09-23 | Twist Bioscience Corporation | SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS |
| WO2016126882A1 (en) | 2015-02-04 | 2016-08-11 | Twist Bioscience Corporation | Methods and devices for de novo oligonucleic acid assembly |
| JP6773687B2 (ja) | 2015-02-17 | 2020-10-21 | エムジーアイ テック カンパニー リミテッドＭＧＩ Ｔｅｃｈ Ｃｏ．， Ｌｔｄ． | 制御された鎖置換を用いてのｄｎａ配列決定 |
| US9981239B2 (en) | 2015-04-21 | 2018-05-29 | Twist Bioscience Corporation | Devices and methods for oligonucleic acid library synthesis |
| EP3307908B1 (en) | 2015-06-09 | 2019-09-11 | Life Technologies Corporation | Methods for molecular tagging |
| HK1258869A1 (zh) | 2015-09-18 | 2019-11-22 | Twist Bioscience Corporation | 寡核酸变体文库及其合成 |
| CN108698012A (zh) | 2015-09-22 | 2018-10-23 | 特韦斯特生物科学公司 | 用于核酸合成的柔性基底 |
| US9895673B2 (en) | 2015-12-01 | 2018-02-20 | Twist Bioscience Corporation | Functionalized surfaces and preparation thereof |
| EP4354140A3 (en) | 2016-02-26 | 2024-07-24 | The Board of Trustees of the Leland Stanford Junior University | Multiplexed single molecule rna visualization with a two-probe proximity ligation system |
| US11085037B2 (en) | 2016-03-23 | 2021-08-10 | Codex Dna, Inc. | Generation of synthetic genomes |
| US11401548B2 (en) | 2016-03-25 | 2022-08-02 | Qiagen Sciences, Llc | Primers with self-complementary sequences for multiple displacement amplification |
| SG11201901563UA (en) | 2016-08-22 | 2019-03-28 | Twist Bioscience Corp | De novo synthesized nucleic acid libraries |
| US10417457B2 (en) | 2016-09-21 | 2019-09-17 | Twist Bioscience Corporation | Nucleic acid based data storage |
| AU2017378492B2 (en) | 2016-12-16 | 2022-06-16 | Twist Bioscience Corporation | Variant libraries of the immunological synapse and synthesis thereof |
| US11550939B2 (en) | 2017-02-22 | 2023-01-10 | Twist Bioscience Corporation | Nucleic acid based data storage using enzymatic bioencryption |
| EP3595674A4 (en) | 2017-03-15 | 2020-12-16 | Twist Bioscience Corporation | BANKS OF VARIANTS OF IMMUNOLOGICAL SYNAPSE AND THEIR SYNTHESIS |
| WO2018214036A1 (zh) * | 2017-05-23 | 2018-11-29 | 深圳华大基因股份有限公司 | 基于滚环扩增的基因组目标区域富集方法及其应用 |
| IL271205B2 (en) | 2017-06-12 | 2025-02-01 | Twist Bioscience Corp | Methods for assembling continuous nucleic acids |
| WO2018231864A1 (en) | 2017-06-12 | 2018-12-20 | Twist Bioscience Corporation | Methods for seamless nucleic acid assembly |
| GB2581620A (en) | 2017-09-11 | 2020-08-26 | Twist Bioscience Corp | GPCR binding proteins and synthesis thereof |
| CA3079613A1 (en) | 2017-10-20 | 2019-04-25 | Twist Bioscience Corporation | Heated nanowells for polynucleotide synthesis |
| GB2585506A (en) | 2018-01-04 | 2021-01-13 | Twist Bioscience Corp | DNA-based digital information storage |
| CN112243463B (zh) | 2018-04-09 | 2024-12-13 | 小利兰·斯坦福大学托管委员会 | 原位基因测序方法 |
| CA3100739A1 (en) | 2018-05-18 | 2019-11-21 | Twist Bioscience Corporation | Polynucleotides, reagents, and methods for nucleic acid hybridization |
| RU2766717C1 (ru) * | 2018-07-30 | 2022-03-15 | Орикиро Дженомикс, Инк. | Способ редактирования днк в бесклеточной системе |
| WO2020055887A1 (en) * | 2018-09-10 | 2020-03-19 | T2 Biosystems, Inc. | Methods and compositions for high sensitivity sequencing in complex samples |
| EP3902816A4 (en) | 2018-12-26 | 2022-09-14 | Twist Bioscience Corporation | HIGHLY ACCURATE POLYNUCLEOTIDE DE NOVO SYNTHESIS |
| AU2020227802A1 (en) | 2019-02-26 | 2021-10-14 | Twist Bioscience Corporation | Variant nucleic acid libraries for antibody optimization |
| JP2022521551A (ja) | 2019-02-26 | 2022-04-08 | ツイスト バイオサイエンス コーポレーション | Ｇｌｐ１受容体の変異体核酸ライブラリ |
| TWI874374B (zh) | 2019-03-25 | 2025-03-01 | 香港中文大學 | 確定循環核酸之線性及環狀形式 |
| AU2020298294A1 (en) | 2019-06-21 | 2022-02-17 | Twist Bioscience Corporation | Barcode-based nucleic acid sequence assembly |
| US12091777B2 (en) | 2019-09-23 | 2024-09-17 | Twist Bioscience Corporation | Variant nucleic acid libraries for CRTH2 |
| EP4034564A4 (en) | 2019-09-23 | 2023-12-13 | Twist Bioscience Corporation | VARIANT NUCLEIC ACID LIBRARIES FOR SINGLE-DOMAIN ANTIBODIES |
| GB202007428D0 (en) | 2020-05-19 | 2020-07-01 | Fabricnano Ltd | Polynucleotide synthesis |
| US20240401132A1 (en) * | 2021-09-22 | 2024-12-05 | The Penn State Research Foundation | Rolling Circle Amplification-Coupled Glass Nanopore Counting of Mild Traumatic Brain Injury-Related Salivary miRNAs |
| GB202114105D0 (en) | 2021-10-01 | 2021-11-17 | Fabricnano Ltd | Nucleotide synthesis |
| WO2024138550A1 (zh) * | 2022-12-29 | 2024-07-04 | 深圳华大智造科技股份有限公司 | 一种核酸扩增方法及其在测序中的用途 |
| CN116875671A (zh) * | 2023-07-18 | 2023-10-13 | 浙江大学 | 用于dna扩增并标记的标签发夹引物 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2048522C1 (ru) * | 1992-10-14 | 1995-11-20 | Институт белка РАН | Способ размножения нуклеиновых кислот, способ их экспрессии и среда для их осуществления |
| WO1994009127A2 (en) | 1992-10-16 | 1994-04-28 | The Government Of The United States Of America As Represented By The Secretary, Department Of Healthand Human Services | Supercoiled minicircle dna as as a unitary promoter vector |
| US5744299A (en) * | 1995-11-03 | 1998-04-28 | Mcw Research Foundation | Human parainfluenza virus-1 assay |
| DK1019496T3 (da) * | 1997-07-07 | 2005-01-10 | Medical Res Council | In vitro-sorteringsmetode |
| US6210882B1 (en) * | 1998-01-29 | 2001-04-03 | Mayo Foundation For Medical Education And Reseach | Rapid thermocycling for sample analysis |
| WO2000015779A2 (en) * | 1998-09-15 | 2000-03-23 | Yale University | Molecular cloning using rolling circle amplification |
| US6617137B2 (en) * | 2001-10-15 | 2003-09-09 | Molecular Staging Inc. | Method of amplifying whole genomes without subjecting the genome to denaturing conditions |
| EP1594950A4 (en) * | 2003-01-29 | 2007-07-25 | 454 Corp | PRODUCT FOR THE PREPARATION OF SIMPLE STRANDED DNA BANKS |
| CU23400A1 (es) | 2003-04-29 | 2009-08-04 | Ct Ingenieria Genetica Biotech | Método para la obtención de minicírculos replicativos de adn para transferencia génica o como inmunomoduladores |
| WO2005029041A2 (en) | 2003-09-19 | 2005-03-31 | Applera Corporation | High density sequence detection methods and apparatus |
-
2006
- 2006-05-01 CN CN200680024024.4A patent/CN101213311B/zh active Active
- 2006-05-01 CA CA2607532A patent/CA2607532C/en active Active
- 2006-05-01 DK DK06751837.3T patent/DK1885880T3/da active
- 2006-05-01 DE DE602006015633T patent/DE602006015633D1/de active Active
- 2006-05-01 EP EP06751837A patent/EP1885880B1/en active Active
- 2006-05-01 AT AT06751837T patent/ATE474937T1/de not_active IP Right Cessation
- 2006-05-01 US US11/919,515 patent/US8497069B2/en active Active
- 2006-05-01 BR BRPI0607661-0A patent/BRPI0607661A2/pt active IP Right Grant
- 2006-05-01 WO PCT/US2006/016349 patent/WO2006119066A2/en not_active Ceased
- 2006-05-01 AU AU2006242387A patent/AU2006242387B2/en active Active
-
2007
- 2007-10-29 IL IL187002A patent/IL187002A/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| CA2607532A1 (en) | 2006-11-09 |
| CN101213311A (zh) | 2008-07-02 |
| AU2006242387A1 (en) | 2006-11-09 |
| CA2607532C (en) | 2013-03-12 |
| IL187002A0 (en) | 2008-02-09 |
| DE602006015633D1 (de) | 2010-09-02 |
| BRPI0607661A2 (pt) | 2009-09-22 |
| CN101213311B (zh) | 2013-02-13 |
| US20090176280A1 (en) | 2009-07-09 |
| EP1885880A2 (en) | 2008-02-13 |
| AU2006242387B2 (en) | 2011-01-06 |
| EP1885880B1 (en) | 2010-07-21 |
| WO2006119066A2 (en) | 2006-11-09 |
| ATE474937T1 (de) | 2010-08-15 |
| IL187002A (en) | 2012-03-29 |
| WO2006119066A3 (en) | 2007-03-15 |
| HK1112490A1 (en) | 2008-09-05 |
| US8497069B2 (en) | 2013-07-30 |
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