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DE69131834D1 - Serinproteasevarianten mit peptidligase aktivität - Google Patents

Serinproteasevarianten mit peptidligase aktivität

Info

Publication number
DE69131834D1
DE69131834D1 DE69131834T DE69131834T DE69131834D1 DE 69131834 D1 DE69131834 D1 DE 69131834D1 DE 69131834 T DE69131834 T DE 69131834T DE 69131834 T DE69131834 T DE 69131834T DE 69131834 D1 DE69131834 D1 DE 69131834D1
Authority
DE
Germany
Prior art keywords
serine protease
ligase activity
versions
ligation
peptide ligase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69131834T
Other languages
English (en)
Other versions
DE69131834T2 (de
Inventor
Lars Abrahmsen
John Burnier
James Wells
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of DE69131834D1 publication Critical patent/DE69131834D1/de
Application granted granted Critical
Publication of DE69131834T2 publication Critical patent/DE69131834T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69131834T 1990-08-09 1991-08-06 Serinproteasevarianten mit peptidligase aktivität Expired - Lifetime DE69131834T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US56602690A 1990-08-09 1990-08-09
PCT/US1991/005480 WO1992002615A1 (en) 1990-08-09 1991-08-06 Serine protease variants having peptide ligase activity

Publications (2)

Publication Number Publication Date
DE69131834D1 true DE69131834D1 (de) 2000-01-13
DE69131834T2 DE69131834T2 (de) 2000-05-31

Family

ID=24261145

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69131834T Expired - Lifetime DE69131834T2 (de) 1990-08-09 1991-08-06 Serinproteasevarianten mit peptidligase aktivität

Country Status (8)

Country Link
US (2) US5403737A (de)
EP (1) EP0542931B1 (de)
JP (1) JP3362847B2 (de)
AT (1) ATE187495T1 (de)
CA (1) CA2087342C (de)
DE (1) DE69131834T2 (de)
DK (1) DK0542931T3 (de)
WO (1) WO1992002615A1 (de)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5629173A (en) * 1990-08-09 1997-05-13 Genentech, Inc. Methods of use of serine protease variants having peptide ligase activity
WO1994018329A2 (en) * 1993-02-04 1994-08-18 Genentech, Inc. Serine protease variants having peptide ligase activity
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6187579B1 (en) * 1993-10-28 2001-02-13 Carlsberg A/S Customized proteases
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US5780285A (en) * 1995-03-03 1998-07-14 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing dibasic residues
US5837516A (en) * 1995-03-03 1998-11-17 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing basic residues
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
US5985627A (en) * 1997-02-28 1999-11-16 Carlsberg Laboratory Modified carboxypeptidase
US5858758A (en) * 1997-05-07 1999-01-12 Incyte Pharmaceuticals, Inc. Human serine protease precursor
ES2368718T3 (es) * 1997-10-23 2011-11-21 Danisco Us Inc. Variantes de subtilisina con múltiples sustituciones.
DE69942910D1 (de) * 1998-01-23 2010-12-16 Univ Toronto Chemisch modifizierte enzyme und methoden zu ihrer herstellung und zum screeningder enzyme
US6395532B1 (en) 1998-01-23 2002-05-28 Genencor International, Inc. Modified enzymes and their use for peptide synthesis
AU749383B2 (en) 1998-07-02 2002-06-27 Genencor International, Inc. Chemically modified proteins with a carbohydrate moiety
AU772427B2 (en) * 1998-11-10 2004-04-29 Genencor International, Inc. Chemically modified mutant serine hydrolases show improved catalytic activity and chiral selectivity
WO2000037658A2 (en) * 1998-12-21 2000-06-29 Genencor International, Inc. Chemically modified enzymes with multiple charged variants
MXPA01010896A (es) * 1999-04-28 2003-06-24 Genencor Int Antagonistas cataliticos especificamente dirigidos al objetivo y usos de los mismos.
US6627744B2 (en) * 1999-07-02 2003-09-30 Genencor International, Inc. Synthesis of glycodendrimer reagents
US6541234B1 (en) * 2000-09-11 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
US6541235B1 (en) * 2000-09-29 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
DE10049673A1 (de) * 2000-10-07 2002-04-25 Univ Leipzig Verfahren zur Herstellung von Peptiden, Peptidmimetika und Proteinen
US20040097720A1 (en) * 2001-04-02 2004-05-20 Bott Richard R. Use of glycodendrimer reagents for inhibiting adhesion by microorganisms
US6713529B2 (en) * 2001-08-10 2004-03-30 Illinois Tool Works, Inc. Phase-change ink composition
US20040241780A1 (en) * 2001-08-24 2004-12-02 Jones J. Bryan Chemically modified mutant enzymes and methods for producing them, and screening them for amidase and /or esterase activity
US20080221027A1 (en) * 2007-03-09 2008-09-11 The Regents Of The University Of California Composition and Methods for the Treatment of Duchene Muscular Dystrophy
CN101808639B (zh) * 2007-09-05 2012-12-05 宝丽制药股份有限公司 抗真菌医药组合物
JP7017409B2 (ja) 2014-10-10 2022-02-08 エンザイペプ ビー．ブイ． 加水分解に対する合成の比が改善したスブチリシン変異体を使用するペプチド断片縮合及び環化
US10752931B2 (en) * 2015-07-09 2020-08-25 Enzypep B.V. Designing an enzymatic peptide fragment condensation strategy
EP3299085A1 (de) 2016-09-26 2018-03-28 Enzypep B.V. Peptidkopplungsmolekül
EP3404036A1 (de) 2017-05-19 2018-11-21 Enzypep B.V. Subtilisinvarianten mit einer mutation in der s2- oder s2'-tasche
US10858414B2 (en) 2018-03-09 2020-12-08 Enzypep B.V. Chemo-enzymatic synthesis of semaglutide, liraglutide and GLP-1
PL3704143T3 (pl) 2018-03-09 2024-04-15 Fresenius Kabi Ipsum S.R.L. Chemo-enzymatyczna synteza liraglutydu, semaglutydu i glp-1
KR20230144058A (ko) 2021-02-12 2023-10-13 프레제니우스 카비 입섬 에스. 알. 엘. 서브틸리신 변이체 및 그의 용도
EP4122944A1 (de) 2021-07-21 2023-01-25 EnzyTag B.V. Enzymatische synthese von zyklischen peptiden
CN120390796A (zh) 2022-12-19 2025-07-29 费森尤斯卡比有限公司 热稳定的枯草杆菌蛋白酶变体及其用途
WO2025058511A1 (en) * 2023-09-12 2025-03-20 Endoprotease Services B.V. Acyltransferase-catalysed modification of peptides

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5155033A (en) * 1989-01-06 1992-10-13 Genencor, Inc. Subtilisins modified at position 225 resulting in a shift in catalytic activity
DE3750916T2 (de) * 1986-04-30 1995-06-01 Genencor Int Mutante einer nicht menschlichen Carbonyl-Hydrolase, für diese kodierende DNS-Sequenzen und Vektoren und durch diese Vektoren transformierte Wirte.

Also Published As

Publication number Publication date
EP0542931B1 (de) 1999-12-08
JP3362847B2 (ja) 2003-01-07
JPH06500019A (ja) 1994-01-06
DK0542931T3 (da) 2000-05-22
CA2087342C (en) 2003-12-23
WO1992002615A1 (en) 1992-02-20
CA2087342A1 (en) 1992-02-10
US5403737A (en) 1995-04-04
DE69131834T2 (de) 2000-05-31
EP0542931A4 (de) 1994-04-13
US5763256A (en) 1998-06-09
ATE187495T1 (de) 1999-12-15
EP0542931A1 (de) 1993-05-26

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