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WO1994018329A3 - Serine protease variants having peptide ligase activity - Google Patents

Serine protease variants having peptide ligase activity Download PDF

Info

Publication number
WO1994018329A3
WO1994018329A3 PCT/US1994/001336 US9401336W WO9418329A3 WO 1994018329 A3 WO1994018329 A3 WO 1994018329A3 US 9401336 W US9401336 W US 9401336W WO 9418329 A3 WO9418329 A3 WO 9418329A3
Authority
WO
WIPO (PCT)
Prior art keywords
protease variants
serine protease
ligase activity
ligation
peptide ligase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1994/001336
Other languages
French (fr)
Other versions
WO1994018329A2 (en
Inventor
Lars Abrahmsen
John Burnier
James A Wells
David Y Jackson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Priority to AU61715/94A priority Critical patent/AU6171594A/en
Publication of WO1994018329A2 publication Critical patent/WO1994018329A2/en
Publication of WO1994018329A3 publication Critical patent/WO1994018329A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Diabetes (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention relates to serine protease variants derived from precursor serine proteases via recombinant and/or chemical methods to form protease variants having improved peptide ligase activity. The invention also includes novel ligation substrates which in combination with the serine protease variants and a second ligation substrate are capable of forming a ligation product. The invention also relates to methods for forming such ligation products and the products formed thereby.
PCT/US1994/001336 1993-02-04 1994-02-02 Serine protease variants having peptide ligase activity Ceased WO1994018329A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU61715/94A AU6171594A (en) 1993-02-04 1994-02-02 Serine protease variants having peptide ligase activity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1344593A 1993-02-04 1993-02-04
US08/013,445 1993-02-04

Publications (2)

Publication Number Publication Date
WO1994018329A2 WO1994018329A2 (en) 1994-08-18
WO1994018329A3 true WO1994018329A3 (en) 1995-02-02

Family

ID=21760005

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1994/001336 Ceased WO1994018329A2 (en) 1993-02-04 1994-02-02 Serine protease variants having peptide ligase activity

Country Status (2)

Country Link
AU (1) AU6171594A (en)
WO (1) WO1994018329A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5858758A (en) * 1997-05-07 1999-01-12 Incyte Pharmaceuticals, Inc. Human serine protease precursor
DE10049673A1 (en) 2000-10-07 2002-04-25 Univ Leipzig Enzymatic synthesis of peptide, its mimic or protein, useful e.g. in drug development, using a modified ligase adapted to a particular ester leaving group
WO2006015879A1 (en) 2004-08-13 2006-02-16 Roche Diagniostics Gmbh C-terminal modification of polypeptides
CN107002110A (en) * 2014-10-10 2017-08-01 恩细贝普有限公司 Condensation and cyclization of peptide fragments with subtilisin variants with improved synthetic hydrolysis ratios
WO2017058114A1 (en) * 2015-10-01 2017-04-06 Nanyang Technological University Butelase-mediated peptide ligation
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN119391659B (en) * 2024-10-30 2025-10-10 天津凯莱英生物科技有限公司 Method for preparing polypeptide ligase mutant and polypeptide
CN119391658B (en) * 2024-10-30 2025-10-10 天津凯莱英生物科技有限公司 Method for preparing polypeptide ligase mutant and polypeptide

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992002615A1 (en) * 1990-08-09 1992-02-20 Genentech, Inc. Serine protease variants having peptide ligase activity

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992002615A1 (en) * 1990-08-09 1992-02-20 Genentech, Inc. Serine protease variants having peptide ligase activity

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
J. ROBERTS AND M. CASERIO: "Basic principles in Organic Chemistry", 1965, W.A. BENJAMIN, NEW YORK *

Also Published As

Publication number Publication date
WO1994018329A2 (en) 1994-08-18
AU6171594A (en) 1994-08-29

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