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CY1105288T1 - Αλληλουχιοειδικος dna ανασυνδυασμος εντος ευκαρυωτικων κυτταρων - Google Patents

Αλληλουχιοειδικος dna ανασυνδυασμος εντος ευκαρυωτικων κυτταρων

Info

Publication number
CY1105288T1
CY1105288T1 CY20061101290T CY061101290T CY1105288T1 CY 1105288 T1 CY1105288 T1 CY 1105288T1 CY 20061101290 T CY20061101290 T CY 20061101290T CY 061101290 T CY061101290 T CY 061101290T CY 1105288 T1 CY1105288 T1 CY 1105288T1
Authority
CY
Cyprus
Prior art keywords
sequence
dna
eukaryotic cells
allelespecific
dna recombination
Prior art date
Application number
CY20061101290T
Other languages
English (en)
Inventor
Peter Dröge
Nicole Christ
Elke Lorbach
Σινγκαπούρη
Original Assignee
Peter Dröge
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Peter Dröge filed Critical Peter Dröge
Publication of CY1105288T1 publication Critical patent/CY1105288T1/el

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A61P21/04—Drugs for disorders of the muscular or neuromuscular system for myasthenia gravis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Diabetes (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Dermatology (AREA)
  • Neurology (AREA)
  • Obesity (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Η παρούσα εφεύρεση σχετίζεται με μέθοδο για τον αλληλουχιοειδικό ανασυνδυασμό του DNA εντός ευκαρυωτικών κυττάρων, περιλαμβάνουσα την εισαγωγή -σε κύτταρο- μίας πρώτης DNA-αλληλουχίας, την εισαγωγή -σε κύτταρο-μίας δεύτερης DNA-αλληλουχίας, και την πραγματοποίηση του αλληλουχιοειδικού ανασυνδυασμού μέσω επίδρασης κάποιας ιντεγκράσης Int του βακτηριοφάγου λ. Προτιμώμενη διαμόρφωση σχετίζεται με μέθοδο περαιτέρω περιλαμβάνουσα την πραγματοποίηση ενός δεύτερου αλληλουχιοειδικού ανασυνδυασμού του DNA μέσω επίδρασης κάποιας Int και κάποιου παράγοντα Xis. Η παρούσα εφεύρεση σχετίζεται περαιτέρω με φορείς κλωνοποίησης και τη χρήση τους ως φαρμακευτικών μέσων.
CY20061101290T 1999-08-30 2006-09-11 Αλληλουχιοειδικος dna ανασυνδυασμος εντος ευκαρυωτικων κυτταρων CY1105288T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19941186A DE19941186A1 (de) 1999-08-30 1999-08-30 Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen
PCT/DE2000/002947 WO2001016345A2 (de) 1999-08-30 2000-08-29 Sequenz-spezifische dna-rekombination in eukaryotischen zellen

Publications (1)

Publication Number Publication Date
CY1105288T1 true CY1105288T1 (el) 2010-03-03

Family

ID=7920135

Family Applications (2)

Application Number Title Priority Date Filing Date
CY20061101290T CY1105288T1 (el) 1999-08-30 2006-09-11 Αλληλουχιοειδικος dna ανασυνδυασμος εντος ευκαρυωτικων κυτταρων
CY20101100065T CY1110590T1 (el) 1999-08-30 2010-01-21 Επιλεκτικος ως προς την αλληλουχια dna-ανασυνδυασμος σε ευκαρυωτικα κυτταρα

Family Applications After (1)

Application Number Title Priority Date Filing Date
CY20101100065T CY1110590T1 (el) 1999-08-30 2010-01-21 Επιλεκτικος ως προς την αλληλουχια dna-ανασυνδυασμος σε ευκαρυωτικα κυτταρα

Country Status (19)

Country Link
US (2) US20030027337A1 (el)
EP (2) EP1214440B1 (el)
JP (1) JP2003520028A (el)
KR (1) KR100490188B1 (el)
CN (1) CN1182247C (el)
AT (2) ATE331802T1 (el)
AU (1) AU776297B2 (el)
CA (1) CA2390526C (el)
CY (2) CY1105288T1 (el)
CZ (1) CZ302620B6 (el)
DE (3) DE19941186A1 (el)
DK (2) DK1681355T3 (el)
ES (2) ES2267567T3 (el)
HK (1) HK1047129B (el)
IL (2) IL148152A0 (el)
MX (1) MX244498B (el)
PT (2) PT1214440E (el)
SK (1) SK286034B6 (el)
WO (1) WO2001016345A2 (el)

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EP1173460B1 (en) 1999-03-02 2009-09-16 Life Technologies Corporation Compositions and methods for use in recombinational cloning of nucleic acids
WO2001042509A1 (en) 1999-12-10 2001-06-14 Invitrogen Corporation Use of multiple recombination sites with unique specificity in recombinational cloning
US7244560B2 (en) 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
EP1390394A4 (en) * 2001-04-19 2004-05-26 Invitrogen Corp COMPOSITIONS AND METHODS FOR RECOMBINANT CLONING OF NUCLEIC ACID MOLECULES
WO2002095055A2 (en) * 2001-05-21 2002-11-28 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
NZ529597A (en) * 2001-05-30 2005-12-23 Chromos Molecular Systems Inc Plant artificial chromosomes, uses thereof and methods of preparing plant artificial chromosomes
US20030119104A1 (en) * 2001-05-30 2003-06-26 Edward Perkins Chromosome-based platforms
JP2005521400A (ja) 2002-03-29 2005-07-21 シンジェンタ パーティシペーションズ アクチェンゲゼルシャフト 植物におけるラムダインテグラーゼ媒介型組換え
AU2003253992A1 (en) * 2002-07-18 2004-02-09 Robert P. Bennett Viral vectors containing recombination sites
US7491539B2 (en) 2002-12-05 2009-02-17 Boehringer Ingelheim Pharma Kg Sequence specific DNA recombination in eukaryotic cells
TW200502390A (en) 2002-11-28 2005-01-16 Peter Droege Sequence specific DNA recombination in eukaryotic cells
WO2005028615A2 (en) * 2003-06-26 2005-03-31 Invitrogen Corporation Methods and compositions for detecting promoter activity and expressing fusion proteins
EP2484687A3 (en) 2003-08-08 2012-11-14 Life Technologies Corporation Methods and compositions for seamless cloning of nucleic acid molecules
US8304189B2 (en) * 2003-12-01 2012-11-06 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same
US7935862B2 (en) 2003-12-02 2011-05-03 Syngenta Participations Ag Targeted integration and stacking of DNA through homologous recombination
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WO2009068645A1 (en) * 2007-11-30 2009-06-04 Boehringer Ingelheim Pharma Gmbh & Co. Kg Novel recombination sequences
CN102604936B (zh) * 2011-01-21 2013-09-04 上海市儿童医院 羊基因组中被噬菌体*c31整合酶识别的dna及其应用
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CN102533741B (zh) * 2011-12-09 2014-09-24 深圳华大基因研究院 猪假attp位点及其用途
US11078493B2 (en) 2016-06-21 2021-08-03 Nanyang Technological University Site-specific DNA recombination
CA3066047A1 (en) 2017-06-14 2018-12-20 Technische Universitat Dresden Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes
WO2023237453A1 (en) * 2022-06-07 2023-12-14 Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement Attp mv4-derived site-specific recombination and its use for integration of sequence of interest

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Also Published As

Publication number Publication date
CN1182247C (zh) 2004-12-29
AU7642900A (en) 2001-03-26
ES2334577T3 (es) 2010-03-12
CY1110590T1 (el) 2015-04-29
US20130133092A1 (en) 2013-05-23
EP1214440B1 (de) 2006-06-28
SK286034B6 (sk) 2008-01-07
DK1681355T3 (da) 2009-12-14
WO2001016345A3 (de) 2001-12-06
DE19941186A1 (de) 2001-03-01
EP1681355B1 (de) 2009-10-21
MX244498B (es) 2007-03-28
EP1214440A2 (de) 2002-06-19
WO2001016345A2 (de) 2001-03-08
EP1681355A1 (de) 2006-07-19
CZ2002756A3 (cs) 2002-06-12
SK3042002A3 (en) 2002-07-02
CZ302620B6 (cs) 2011-08-03
DE50015772D1 (de) 2009-12-03
CN1387576A (zh) 2002-12-25
ATE446373T1 (de) 2009-11-15
KR20020057953A (ko) 2002-07-12
DK1214440T3 (da) 2006-10-30
MXPA02002036A (es) 2003-08-20
DE50013093D1 (de) 2006-08-10
PT1681355E (pt) 2010-01-27
CA2390526C (en) 2010-10-19
JP2003520028A (ja) 2003-07-02
KR100490188B1 (ko) 2005-05-17
IL148152A0 (en) 2002-09-12
PT1214440E (pt) 2006-11-30
CA2390526A1 (en) 2001-03-08
IL148152A (en) 2007-10-31
HK1047129A1 (en) 2003-02-07
US20030027337A1 (en) 2003-02-06
HK1047129B (zh) 2006-10-20
ES2267567T3 (es) 2007-03-16
AU776297B2 (en) 2004-09-02
ATE331802T1 (de) 2006-07-15

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