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CA2419282A1 - Procede d'accumulation de produit genique etranger a forte concentration dans des semences vegetales - Google Patents

Procede d'accumulation de produit genique etranger a forte concentration dans des semences vegetales Download PDF

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Publication number
CA2419282A1
CA2419282A1 CA002419282A CA2419282A CA2419282A1 CA 2419282 A1 CA2419282 A1 CA 2419282A1 CA 002419282 A CA002419282 A CA 002419282A CA 2419282 A CA2419282 A CA 2419282A CA 2419282 A1 CA2419282 A1 CA 2419282A1
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CA
Canada
Prior art keywords
foreign gene
untranslated region
plant
gene
promoter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002419282A
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English (en)
Other versions
CA2419282C (fr
Inventor
Fumio Takaiwa
Yoshifumi Tada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Agrobiological Sciences
National Agriculture and Bio Oriented Research Organization NARO
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Individual
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Filing date
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Application filed by Individual filed Critical Individual
Publication of CA2419282A1 publication Critical patent/CA2419282A1/fr
Application granted granted Critical
Publication of CA2419282C publication Critical patent/CA2419282C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'utilisation de la région 5' non traductionnelle d'un gène de protéine de semence permet de mettre au point efficacement un vecteur capable d'exprimer un gène étranger à forte concentration dans une semence végétale. L'utilisation d'un mutant déficient en protéine de semence comme cible pour le transfert d'un gène étranger permet en outre d'accumuler efficacement un produit génique étranger à forte concentration dans une semence végétale.
CA2419282A 2000-08-22 2001-08-17 Procede d'accumulation de produit genique etranger a forte concentration dans des semences vegetales Expired - Fee Related CA2419282C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2000-251606 2000-08-22
JP2000251606A JP4565231B2 (ja) 2000-08-22 2000-08-22 外来遺伝子産物を植物の種子中に高度に蓄積させる方法
PCT/JP2001/007087 WO2002016604A1 (fr) 2000-08-22 2001-08-17 Procede d'accumulation de produit genique etranger a forte concentration dans des semences vegetales

Publications (2)

Publication Number Publication Date
CA2419282A1 true CA2419282A1 (fr) 2003-02-11
CA2419282C CA2419282C (fr) 2012-10-16

Family

ID=18740982

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2419282A Expired - Fee Related CA2419282C (fr) 2000-08-22 2001-08-17 Procede d'accumulation de produit genique etranger a forte concentration dans des semences vegetales

Country Status (9)

Country Link
US (1) US7473825B2 (fr)
EP (1) EP1312672B1 (fr)
JP (1) JP4565231B2 (fr)
KR (1) KR20030028580A (fr)
CN (1) CN1471578A (fr)
AU (2) AU7876501A (fr)
CA (1) CA2419282C (fr)
ES (1) ES2400135T3 (fr)
WO (1) WO2002016604A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7728192B2 (en) * 1999-12-10 2010-06-01 Kimmo Koivu Process for converting storage reserves of dicotyledonous seeds into compositions comprising one or more gene products
AU2003289283B2 (en) 2002-12-20 2008-05-08 Incorporated Administrative Agency National Agriculture And Bio-Oriented Research Organization Plant with reduced protein content in seed, method of constructing the same and method of using the same
WO2004087910A1 (fr) * 2003-03-28 2004-10-14 National Institute Of Agrobiological Sciences Procede de production d'un organe de stockage vegetal avec une production elevee de proteines de recombinaison et nouvelle proteine de recombinaison
US20070143872A1 (en) * 2003-08-27 2007-06-21 Orf Liftaekni Hf. Enhancing accumulation of heterologous polypeptides in plant seeds through targeted suppression of endogenous storage proteins
JP4019147B2 (ja) 2003-10-31 2007-12-12 独立行政法人農業生物資源研究所 種子特異的プロモーターおよびその利用
EP1808491A1 (fr) * 2006-01-13 2007-07-18 Meristem Therapeutics S.A. Plantes génétiquement modifiées avec une teneur modifiée d'une proteine seminale de stockage
JP5158639B2 (ja) 2008-04-11 2013-03-06 独立行政法人農業生物資源研究所 植物の内胚乳に特異的に発現する遺伝子および該遺伝子のプロモーター、並びにそれらの利用
US20100313307A1 (en) * 2008-06-28 2010-12-09 Donald Danforth Plant Science Center Protein production and storage in plants
KR101383340B1 (ko) * 2012-06-15 2014-04-10 건국대학교 산학협력단 애기장대 슛 시스템에서 성장 및 발달을 조절하는 방법
CN109486851B (zh) * 2018-10-12 2022-04-01 武汉禾元生物科技股份有限公司 一种提高胚乳生物反应器中重组蛋白表达水平的方法
CN119162181A (zh) * 2024-10-24 2024-12-20 浙江大学 一种用于提高外源基因在植物种子中表达量的重组启动子及方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2811889A (en) * 1987-10-20 1989-05-23 Plant Genetic Systems N.V. A process for the production of biologically active peptide via the expression of modified storage seed protein genes in transgenic plants
CA2092069A1 (fr) * 1992-03-27 1993-09-28 Asako Iida Plasmide d'expression pour semence
PL310524A1 (en) * 1993-03-02 1995-12-27 Du Pont Improved fodder plants enriched with sulphuric amino acids and method of improving fodder plants
JPH07213185A (ja) 1994-02-03 1995-08-15 Sumitomo Chem Co Ltd 含硫アミノ酸含量の改良植物および改良方法
DE19601365A1 (de) 1996-01-16 1997-07-17 Planttec Biotechnologie Gmbh Nucleinsäuremoleküle aus Pflanzen codierend Enzyme, die an der Stärkesynthese beteiligt sind
JP2001503964A (ja) 1996-05-29 2001-03-27 ヘキスト シェリング アグレボ ゲーエムベーハー デンプン合成に関与するコムギ由来酵素をコードする核酸分子
DE19653176A1 (de) 1996-12-19 1998-06-25 Planttec Biotechnologie Gmbh Neue Nucleinsäuremoleküle aus Mais und ihre Verwendung zur Herstellung einer modifizierten Stärke
JP3030339B2 (ja) * 1998-08-07 2000-04-10 農林水産省農業生物資源研究所長 ダイズグリシニンを発現するトランスジェニック植物
AU2023700A (en) 1998-11-10 2000-05-29 Maxygen, Inc. Modified ribulose 1,5-bisphosphate carboxylase/oxygenase
US6235976B1 (en) * 1999-01-29 2001-05-22 Pioneer Hi-Bred International, Inc. Soybean variety 94B53
US7417178B2 (en) * 2000-05-02 2008-08-26 Ventria Bioscience Expression of human milk proteins in transgenic plants
US7741533B2 (en) * 2000-11-07 2010-06-22 Pioneer Hi-Bred International, Inc. Grain quality through altered expression of seed proteins

Also Published As

Publication number Publication date
AU7876501A (en) 2002-03-04
JP2002058492A (ja) 2002-02-26
EP1312672A4 (fr) 2006-06-28
WO2002016604A1 (fr) 2002-02-28
EP1312672A1 (fr) 2003-05-21
US7473825B2 (en) 2009-01-06
KR20030028580A (ko) 2003-04-08
AU2001278765C1 (en) 2006-05-11
AU2001278765B2 (en) 2005-08-18
CN1471578A (zh) 2004-01-28
ES2400135T3 (es) 2013-04-05
JP4565231B2 (ja) 2010-10-20
CA2419282C (fr) 2012-10-16
EP1312672B1 (fr) 2012-11-21
US20040031075A1 (en) 2004-02-12

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