[go: up one dir, main page]

AU2019262048B2 - High throughput multiomics sample analysis - Google Patents

High throughput multiomics sample analysis

Info

Publication number
AU2019262048B2
AU2019262048B2 AU2019262048A AU2019262048A AU2019262048B2 AU 2019262048 B2 AU2019262048 B2 AU 2019262048B2 AU 2019262048 A AU2019262048 A AU 2019262048A AU 2019262048 A AU2019262048 A AU 2019262048A AU 2019262048 B2 AU2019262048 B2 AU 2019262048B2
Authority
AU
Australia
Prior art keywords
sequence
fragments
dsdna
label
barcodes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2019262048A
Other languages
English (en)
Other versions
AU2019262048A1 (en
Inventor
Christina FAN
Elisabeth Marie Walczak
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Publication of AU2019262048A1 publication Critical patent/AU2019262048A1/en
Application granted granted Critical
Publication of AU2019262048B2 publication Critical patent/AU2019262048B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
AU2019262048A 2018-05-03 2019-05-01 High throughput multiomics sample analysis Active AU2019262048B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862666483P 2018-05-03 2018-05-03
US62/666,483 2018-05-03
PCT/US2019/030245 WO2019213294A1 (en) 2018-05-03 2019-05-01 High throughput multiomics sample analysis

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2025275142 Division 2019-05-01

Publications (2)

Publication Number Publication Date
AU2019262048A1 AU2019262048A1 (en) 2020-11-12
AU2019262048B2 true AU2019262048B2 (en) 2025-09-04

Family

ID=66530511

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2019262048A Active AU2019262048B2 (en) 2018-05-03 2019-05-01 High throughput multiomics sample analysis

Country Status (8)

Country Link
US (3) US11773441B2 (de)
EP (2) EP4234717A3 (de)
JP (2) JP7407128B2 (de)
CN (1) CN112272710A (de)
AU (1) AU2019262048B2 (de)
CA (1) CA3097976A1 (de)
ES (1) ES2945191T3 (de)
WO (1) WO2019213294A1 (de)

Families Citing this family (100)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
GB2513024B (en) 2012-02-27 2016-08-31 Cellular Res Inc A clonal amplification method
GB2546833B (en) 2013-08-28 2018-04-18 Cellular Res Inc Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels
EP3262192B1 (de) 2015-02-27 2020-09-16 Becton, Dickinson and Company Räumlich adressierbare molekulare barcodierung
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
EP3286326B1 (de) 2015-04-23 2025-01-22 Becton, Dickinson and Company Verfahren zur gesamttranskriptomamplifikation
EP3347465B1 (de) 2015-09-11 2019-06-26 Cellular Research, Inc. Verfahren und zusammensetzung zur standardisierung von nukleinsäure bibliothek
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
CN109791157B (zh) 2016-09-26 2022-06-07 贝克顿迪金森公司 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
WO2018144240A1 (en) 2017-02-01 2018-08-09 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
AU2018281745B2 (en) 2017-06-05 2022-05-19 Becton, Dickinson And Company Sample indexing for single cells
ES2945191T3 (es) 2018-05-03 2023-06-29 Becton Dickinson Co Análisis de muestras multiómicas de alto rendimiento
EP3788170B1 (de) 2018-05-03 2025-01-01 Becton, Dickinson and Company Molekulare barcodierung auf gegenüberliegenden transkriptenden
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
EP4471156A3 (de) 2018-10-01 2025-02-26 Becton, Dickinson and Company Bestimmung von 5'-transkriptsequenzen
WO2020097315A1 (en) 2018-11-08 2020-05-14 Cellular Research, Inc. Whole transcriptome analysis of single cells using random priming
FI3887540T3 (fi) 2018-11-30 2023-09-14 Illumina Inc Useiden analyyttien analyysi käyttäen yhtä määritystä
WO2020123320A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Imaging system hardware
CN113195717A (zh) 2018-12-13 2021-07-30 贝克顿迪金森公司 单细胞全转录组分析中的选择性延伸
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020154247A1 (en) 2019-01-23 2020-07-30 Cellular Research, Inc. Oligonucleotides associated with antibodies
EP3924506A1 (de) 2019-02-14 2021-12-22 Becton Dickinson and Company Hybride gezielte und gesamttranskriptomamplifikation
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
CN114051534B (zh) 2019-07-22 2025-02-21 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
EP4018001A4 (de) * 2019-08-19 2023-09-13 Universal Sequencing Technology Corporation Verfahren und zusammensetzungen zur verfolgung des nukleinsäurefragmentursprungs zur nukleinsäuresequenzierung
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
EP4407041A3 (de) 2019-11-08 2024-09-25 Becton Dickinson and Company Verwendung von random-rpiming zur gewinnung von v(d)j-informationen in voller länge für immunrepertoiresequenzierung
EP4667585A2 (de) 2019-12-23 2025-12-24 10X Genomics, Inc. Verfahren zur räumlichen analyse unter verwendung von rna-template-ligation
CN115038794A (zh) 2019-12-23 2022-09-09 10X基因组学有限公司 在基于分区的测定中使用固定生物样品的组合物和方法
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
EP4471155A3 (de) 2020-01-29 2024-12-18 Becton, Dickinson and Company Strichcodierte bohrlöcher zur räumlichen abbildung einzelner zellen durch sequenzierung
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US20210246492A1 (en) * 2020-02-12 2021-08-12 Becton, Dickinson And Company Intracellular abseq
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
CN115151810A (zh) 2020-02-25 2022-10-04 贝克顿迪金森公司 实现使用单细胞样品作为单色补偿对照的双特异性探针
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
EP4139485B1 (de) 2020-04-22 2023-09-06 10X Genomics, Inc. Verfahren zur räumlichen analyse unter verwendung von gezieltem rna-abbau
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司(Us) 用于免疫组库谱分析的引物
WO2021236625A1 (en) 2020-05-19 2021-11-25 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
EP4600376A3 (de) 2020-06-02 2025-10-22 10X Genomics, Inc. Räumliche transkriptomik für antigenrezeptoren
AU2021283174A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Nucleic acid library methods
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4165207B1 (de) 2020-06-10 2024-09-25 10X Genomics, Inc. Verfahren zur bestimmung der position eines analyten in einer biologischen probe
US12435363B1 (en) 2020-06-10 2025-10-07 10X Genomics, Inc. Materials and methods for spatial transcriptomics
AU2021294334A1 (en) * 2020-06-25 2023-02-02 10X Genomics, Inc. Spatial analysis of DNA methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12391940B2 (en) 2020-07-31 2025-08-19 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
EP4491742A3 (de) 2020-09-18 2025-05-21 10x Genomics, Inc. Probenhandhabungsvorrichtung und bildregistrierungsverfahren
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
JP2023551072A (ja) * 2020-11-25 2023-12-06 アリダ・バイオサイエンシーズ・インコーポレイテッド Rnaおよびdna修飾の多重プロファイリング
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4294571B8 (de) 2021-02-19 2024-07-10 10X Genomics, Inc. Verfahren zur verwendung einer modularen testträgervorrichtung
EP4301870B1 (de) 2021-03-18 2025-01-15 10X Genomics, Inc. Multiplex-erfassung von gen- und proteinexpression aus einer biologischen probe
CN117136240A (zh) * 2021-03-19 2023-11-28 巴黎科学与文学联大 用于选择性分析细胞或细胞器的方法
EP4305196B1 (de) 2021-04-14 2025-04-02 10X Genomics, Inc. Verfahren zur messung der fehllokalisierung eines analyten
WO2022236054A1 (en) 2021-05-06 2022-11-10 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2022253288A1 (zh) * 2021-06-03 2022-12-08 广州燃石医学检验所有限公司 一种甲基化测序方法和装置
EP4196605B1 (de) 2021-09-01 2024-12-04 10X Genomics, Inc. Verfahren zur blockierung einer erfassungssonde auf einer räumlichen anordnung
EP4402280A1 (de) * 2021-09-14 2024-07-24 Becton, Dickinson and Company Vollständige einzelzellen-rna-sequenzierung
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
JP2024541478A (ja) 2021-11-24 2024-11-08 アリダ・バイオサイエンシーズ・インコーポレイテッド 人工表面を用いたrnaおよびdna解析
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
EP4441711A1 (de) 2021-12-20 2024-10-09 10X Genomics, Inc. Selbsttest für eine pathologie-/histologie-objektträgerbildgebungsvorrichtung
AU2024232585A1 (en) * 2023-03-07 2025-08-28 Nanohyb, Llc Method for native strand sequencing of enriched genomic regions
WO2025096912A1 (en) * 2023-11-03 2025-05-08 University Of Massachusetts Methods of assessing rna contamination and integrity
WO2025122621A1 (en) * 2023-12-04 2025-06-12 North Carolina State University Compositions and methods related to aptamers and aptamer-based sensors
WO2025237407A1 (en) * 2024-05-17 2025-11-20 Beijing Changping Laboratory Methods for spatial epigenomic analysis within single cells of tissue sample
US20250382585A1 (en) 2024-06-13 2025-12-18 Nippon Becton Dickinson Company Ltd Tumor and tissue preservation reagent

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016061517A2 (en) * 2014-10-17 2016-04-21 Illumina Cambridge Limited Contiguity preserving transposition

Family Cites Families (624)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4510244A (en) 1980-04-17 1985-04-09 The Board Of Trustees Of The Leland Stanford Jr. University Cell labeling with antigen-coupled microspheres
US4725536A (en) 1985-09-19 1988-02-16 Genetics Institute, Inc. Reagent polynucleotide complex with multiple target binding regions, and kit and methods
US6150517A (en) 1986-11-24 2000-11-21 Gen-Probe Methods for making oligonucleotide probes for the detection and/or quantitation of non-viral organisms
FI891436L (fi) 1987-07-31 1989-03-23 Univ Leland Stanford Junior Selektivt flerfaldigande av targetpolynukleotidsekvenser.
US5149625A (en) 1987-08-11 1992-09-22 President And Fellows Of Harvard College Multiplex analysis of DNA
US5656731A (en) 1987-10-15 1997-08-12 Chiron Corporation Nucleic acid-amplified immunoassay probes
US5124246A (en) 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
JPH04501353A (ja) 1988-07-26 1992-03-12 ジエネラブス・テクノロジーズ・インコーポレイテツド Rna及びdna増幅法
US5871928A (en) 1989-06-07 1999-02-16 Fodor; Stephen P. A. Methods for nucleic acid analysis
US6551784B2 (en) 1989-06-07 2003-04-22 Affymetrix Inc Method of comparing nucleic acid sequences
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5925525A (en) 1989-06-07 1999-07-20 Affymetrix, Inc. Method of identifying nucleotide differences
US6309822B1 (en) 1989-06-07 2001-10-30 Affymetrix, Inc. Method for comparing copy number of nucleic acid sequences
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US6040138A (en) 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5200314A (en) 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
JP3164814B2 (ja) 1990-08-27 2001-05-14 科学技術振興事業団 均一化cDNAライブラリーの作製法
EP0834575B1 (de) 1990-12-06 2001-11-28 Affymetrix, Inc. (a Delaware Corporation) Identifizierung von Nukleinsäuren in Proben
JPH04337446A (ja) 1991-05-15 1992-11-25 Hitachi Ltd 微粒子計測方法、定量方法および微粒子計測装置
US5981179A (en) 1991-11-14 1999-11-09 Digene Diagnostics, Inc. Continuous amplification reaction
US5424413A (en) 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
US5573905A (en) 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
US5981176A (en) 1992-06-17 1999-11-09 City Of Hope Method of detecting and discriminating between nucleic acid sequences
WO1995000530A1 (en) 1993-06-25 1995-01-05 Affymax Technologies N.V. Hybridization and sequencing of nucleic acids
US6087186A (en) 1993-07-16 2000-07-11 Irori Methods and apparatus for synthesizing labeled combinatorial chemistry libraries
US5500356A (en) 1993-08-10 1996-03-19 Life Technologies, Inc. Method of nucleic acid sequence selection
US6309823B1 (en) 1993-10-26 2001-10-30 Affymetrix, Inc. Arrays of nucleic acid probes for analyzing biotransformation genes and methods of using the same
US5681697A (en) 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5714330A (en) 1994-04-04 1998-02-03 Lynx Therapeutics, Inc. DNA sequencing by stepwise ligation and cleavage
US6495518B1 (en) 1994-06-13 2002-12-17 Vanderbilt University Method for importing biologically active molecules into cells
US6090592A (en) 1994-08-03 2000-07-18 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid on supports
GB2293238A (en) 1994-09-13 1996-03-20 Inceltec Ltd Primers for replication and/or amplification reactions
WO1998053300A2 (en) 1997-05-23 1998-11-26 Lynx Therapeutics, Inc. System and apparaus for sequential processing of analytes
US5695934A (en) 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US6013445A (en) 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US6600996B2 (en) 1994-10-21 2003-07-29 Affymetrix, Inc. Computer-aided techniques for analyzing biological sequences
EP0709466B1 (de) 1994-10-28 2006-09-27 Gen-Probe Incorporated Zusammensetzungen und Verfahren für die gleichzeitige Detektion und Quantifizierung von einer Mehrheit spezifischer Nuklein Säure Sequenzen
CN1181720A (zh) 1995-04-25 1998-05-13 伊萝莉公司 可远程编程的存储器材料及其应用
US5648245A (en) 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US5968740A (en) 1995-07-24 1999-10-19 Affymetrix, Inc. Method of Identifying a Base in a Nucleic Acid
US5763175A (en) 1995-11-17 1998-06-09 Lynx Therapeutics, Inc. Simultaneous sequencing of tagged polynucleotides
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US6613544B1 (en) 1995-12-22 2003-09-02 Amgen Inc. Osteoprotegerin
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US5830712A (en) 1996-02-06 1998-11-03 Allelix Biopharmaceuticals Inc. Selective template deletion method
US6458530B1 (en) 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
WO1997045559A1 (en) 1996-05-29 1997-12-04 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
JPH1021373A (ja) 1996-07-05 1998-01-23 Fuji Photo Film Co Ltd 画像解析装置
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US7169556B2 (en) 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US20020172953A1 (en) 1996-07-29 2002-11-21 Mirkin Chad A. Movement of biomolecule-coated nanoparticles in an electric field
US6984491B2 (en) 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6100029A (en) 1996-08-14 2000-08-08 Exact Laboratories, Inc. Methods for the detection of chromosomal aberrations
WO1998015644A2 (en) 1996-09-27 1998-04-16 The Chinese University Of Hong Kong Parallel polynucleotide sequencing method
US6124092A (en) 1996-10-04 2000-09-26 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
US6117631A (en) 1996-10-29 2000-09-12 Polyprobe, Inc. Detection of antigens via oligonucleotide antibody conjugates
US6046005A (en) 1997-01-15 2000-04-04 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group
US5866366A (en) 1997-07-01 1999-02-02 Smithkline Beecham Corporation gidB
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US6399334B1 (en) 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
AU1603199A (en) 1997-12-03 1999-06-16 Curagen Corporation Methods and devices for measuring differential gene expression
US6265163B1 (en) 1998-01-09 2001-07-24 Lynx Therapeutics, Inc. Solid phase selection of differentially expressed genes
US6117986A (en) 1998-06-10 2000-09-12 Intergen Company, L.P. Pyrimidines linked to a quencher
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6653077B1 (en) 1998-09-04 2003-11-25 Lynx Therapeutics, Inc. Method of screening for genetic polymorphism
US6480791B1 (en) 1998-10-28 2002-11-12 Michael P. Strathmann Parallel methods for genomic analysis
US6197554B1 (en) 1998-11-20 2001-03-06 Shi-Lung Lin Method for generating full-length cDNA library from single cells
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US20020019005A1 (en) 1999-02-18 2002-02-14 Arcaris, Inc. Process for identification of genes encoding proteins having cell proliferation-promoting activity
US6629040B1 (en) 1999-03-19 2003-09-30 University Of Washington Isotope distribution encoded tags for protein identification
JP2002539849A (ja) 1999-03-26 2002-11-26 ホワイトヘッド インスチチュート フォアー バイオメディカル リサーチ ユニバーサルアレイ
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
CA2644688C (en) 1999-04-20 2012-11-13 Kankyo Engineering Co., Ltd. Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analysing data obtained by the method
US6372813B1 (en) 1999-06-25 2002-04-16 Motorola Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays
US6326148B1 (en) 1999-07-12 2001-12-04 The Regents Of The University Of California Detection of copy number changes in colon cancer
US6440706B1 (en) 1999-08-02 2002-08-27 Johns Hopkins University Digital amplification
AU783873B2 (en) 1999-09-13 2005-12-15 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
JP2001078768A (ja) 1999-09-16 2001-03-27 Otsuka Pharmaceut Co Ltd 遺伝子発現の測定法
SE9903988D0 (sv) 1999-11-03 1999-11-03 Amersham Pharm Biotech Ab Method of analysis
US6489114B2 (en) 1999-12-17 2002-12-03 Bio Merieux Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby
JP4860869B2 (ja) 1999-12-29 2012-01-25 オックスフォード ジーン テクノロジー アイピー リミティド 固相支持体上の複数のポリヌクレオチドを増幅し、検出する方法
US7022479B2 (en) 2000-01-24 2006-04-04 Compound Therapeutics, Inc. Sensitive, multiplexed diagnostic assays for protein analysis
JP2003520050A (ja) 2000-01-24 2003-07-02 フィロス インク. タンパク質分析のための高感度多重化診断アッセイ法
US6235483B1 (en) 2000-01-31 2001-05-22 Agilent Technologies, Inc. Methods and kits for indirect labeling of nucleic acids
EP1990428B1 (de) 2000-02-07 2010-12-22 Illumina, Inc. Nukleinsäuredetektionsverfahren mit universellem Priming
US20050214825A1 (en) 2000-02-07 2005-09-29 John Stuelpnagel Multiplex sample analysis on universal arrays
GB2364054B (en) 2000-03-24 2002-05-29 Smithkline Beecham Corp Method of amplifying quinolone-resistance-determining-regions and identifying polymorphic variants thereof
US7348141B2 (en) 2000-03-29 2008-03-25 Lgc Limited Hybridization beacon and method of rapid sequence detection and discrimination
US6613520B2 (en) 2000-04-10 2003-09-02 Matthew Ashby Methods for the survey and genetic analysis of populations
US20030207300A1 (en) 2000-04-28 2003-11-06 Matray Tracy J. Multiplex analytical platform using molecular tags
US7630063B2 (en) 2000-08-02 2009-12-08 Honeywell International Inc. Miniaturized cytometer for detecting multiple species in a sample
US6511809B2 (en) 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
US6531283B1 (en) 2000-06-20 2003-03-11 Molecular Staging, Inc. Protein expression profiling
AU8288101A (en) 2000-07-07 2002-01-21 Visigen Biotechnologies Inc Real-time sequence determination
AU2001281248A1 (en) 2000-08-11 2002-03-13 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6934408B2 (en) 2000-08-25 2005-08-23 Amnis Corporation Method and apparatus for reading reporter labeled beads
CA2356123A1 (en) 2000-08-25 2002-02-25 Riken Method of preparing normalized and/or subtracted cdna
AU2002246612B2 (en) 2000-10-24 2007-11-01 The Board Of Trustees Of The Leland Stanford Junior University Direct multiplex characterization of genomic DNA
WO2002046472A2 (en) 2000-12-08 2002-06-13 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US20020142345A1 (en) 2000-12-22 2002-10-03 Nelsen Anita J. Methods for encoding and decoding complex mixtures in arrayed assays
US20030049616A1 (en) 2001-01-08 2003-03-13 Sydney Brenner Enzymatic synthesis of oligonucleotide tags
AU2002249116A1 (en) 2001-01-11 2002-09-19 Lion Bioscience Ag Gene library for screening methods
AU2002229435B2 (en) 2001-01-25 2007-06-07 Luminex Molecular Diagnostics, Inc. Polynucleotides for use as tags and tag complements, manufacture and use thereof
NZ523167A (en) 2001-03-09 2006-08-31 Nugen Technologies Inc Methods, compositions and kits for amplifying RNA sequences of interest which employ an RNA/DNA composite primer
US7027932B2 (en) 2001-03-21 2006-04-11 Olympus Optical Co., Ltd. Biochemical examination method
JP2005233974A (ja) 2001-03-21 2005-09-02 Olympus Corp 生化学的検査方法
ATE431853T1 (de) 2001-03-28 2009-06-15 Nanosphere Inc Auf oligonukleotid-modifizierten partikeln basierende bio-strichcodes
US20030170675A1 (en) 2001-04-11 2003-09-11 The Gov't Of The U.S Of America As Represented By The Secretary Of The Dept. Of Health & Human Serv. Methods of manipulating nucleic acids
EP1377683A4 (de) 2001-04-11 2004-09-08 Us Gov Health & Human Serv Modifizierte zufallsprimer zur sondenmarkierung
AUPR480901A0 (en) 2001-05-04 2001-05-31 Genomics Research Partners Pty Ltd Diagnostic method for assessing a condition of a performance animal
US6849404B2 (en) 2001-05-07 2005-02-01 Bioneer Corporation Polymerase chain reaction of DNA of which base sequence is completely unidentified
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
CA2450139A1 (en) 2001-06-11 2002-12-19 Illumina, Inc Multiplexed detection methods
JP4244534B2 (ja) 2001-06-12 2009-03-25 横河電機株式会社 バイオチップ
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
US6830931B2 (en) 2001-07-12 2004-12-14 Automated Cell, Inc. Method and apparatus for monitoring of proteins and cells
WO2006071776A2 (en) 2004-12-23 2006-07-06 Ge Healthcare Bio-Sciences Corp. Ligation-based rna amplification
AU2002363062B2 (en) 2001-10-09 2007-03-22 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
AU2002360272A1 (en) 2001-10-10 2003-04-22 Superarray Bioscience Corporation Detecting targets by unique identifier nucleotide tags
US20030077611A1 (en) 2001-10-24 2003-04-24 Sention Methods and systems for dynamic gene expression profiling
EP2196544A1 (de) 2001-11-21 2010-06-16 Applied Biosystems, LLC Kit für Ligationsdetektionsreaktionen unter Verwendung von kodierten Markern
US20030175749A1 (en) 2001-12-08 2003-09-18 Jong-Yoon Chun Annealing control primer and its uses
AU2003202026A1 (en) 2002-01-16 2003-09-02 Dynal Biotech Asa Method for isolating nucleic acids and protein from a single sample
CA2474864A1 (en) 2002-01-29 2003-08-07 Patrik Ernfors Methods and means for amplifying nucleic acid
WO2003069421A2 (en) 2002-02-14 2003-08-21 Immunivest Corporation Methods and algorithms for cell enumeration in a low-cost cytometer
US20030186251A1 (en) 2002-04-01 2003-10-02 Brookhaven Science Associates, Llc Genome sequence tags
US20050175993A1 (en) 2002-04-12 2005-08-11 Chia-Lin Wei Method for making full-length coding sequence cDNA libraries
US20070178478A1 (en) 2002-05-08 2007-08-02 Dhallan Ravinder S Methods for detection of genetic disorders
US6808906B2 (en) 2002-05-08 2004-10-26 Rigel Pharmaceuticals, Inc. Directionally cloned random cDNA expression vector libraries, compositions and methods of use
GB0212391D0 (en) 2002-05-29 2002-07-10 Axis Shield Asa Assay
US9371559B2 (en) 2002-06-20 2016-06-21 The Regents Of The University Of California Compositions for detection and analysis of polynucleotides using light harvesting multichromophores
US20050019776A1 (en) 2002-06-28 2005-01-27 Callow Matthew James Universal selective genome amplification and universal genotyping system
WO2004003219A2 (en) 2002-06-28 2004-01-08 Igen International, Inc. Improved assay systems and components
US7192700B2 (en) 2002-12-20 2007-03-20 Orchid Cellmark Inc. Methods and compositions for conducting primer extension and polymorphism detection reactions
AU2003269968A1 (en) 2002-08-16 2004-03-11 Clinical Microarrays, Inc. Substrates for isolating, reacting and microscopically analyzing materials
EA007653B1 (ru) 2002-08-26 2006-12-29 Дзе Риджентс Оф Дзе Юниверсити Оф Калифорния Способы и композиции обнаружения и анализа полинуклеотидов с помощью светособирающих полихромофоров
EP2143803B1 (de) 2002-09-03 2016-04-13 Quanta Biosciences, Inc. Verbesserte Zusammensetzungen und Verfahren zur cDNA-Synthese
US7361821B2 (en) 2002-09-20 2008-04-22 Intel Corporation Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading
CA2500129C (en) 2002-09-30 2011-03-29 F. Hoffmann-La Roche Ag Oligonucleotides for genotyping thymidylate synthase gene
AU2003299541A1 (en) 2002-10-02 2004-05-25 California Institute Of Technology Microfluidic nucleic acid analysis
US7822555B2 (en) 2002-11-11 2010-10-26 Affymetrix, Inc. Methods for identifying DNA copy number changes
WO2004044225A2 (en) 2002-11-11 2004-05-27 Affymetrix, Inc. Methods for identifying dna copy number changes
US7704687B2 (en) 2002-11-15 2010-04-27 The Johns Hopkins University Digital karyotyping
AU2003302770B2 (en) 2002-12-20 2007-07-12 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA
WO2004066185A1 (en) 2003-01-23 2004-08-05 U.S. Genomics, Inc. Methods for analyzing polymer populations
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
US7718403B2 (en) 2003-03-07 2010-05-18 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
US7269518B2 (en) 2003-04-30 2007-09-11 Agilent Technologies, Inc. Chemical array reading
US20040259118A1 (en) 2003-06-23 2004-12-23 Macevicz Stephen C. Methods and compositions for nucleic acid sequence analysis
CA2531105C (en) 2003-07-05 2015-03-17 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
AU2004259851A1 (en) 2003-07-24 2005-02-03 Qiagen Gmbh Method for the reverse transcription and/or amplification of nucleic acids
JP2007502116A (ja) 2003-08-13 2007-02-08 アフィメトリックス インコーポレイテッド 核酸サンプルを調製するための方法およびキット
GB0319332D0 (en) 2003-08-16 2003-09-17 Astrazeneca Ab Amplification
US20050048498A1 (en) 2003-08-29 2005-03-03 Applera Corporation Compositions, methods, and kits for assembling probes
US7341837B2 (en) 2003-09-02 2008-03-11 Lawton Robert L Soluble analyte detection and amplification
US7354706B2 (en) 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
ATE461292T1 (de) 2003-09-10 2010-04-15 Althea Technologies Inc Erstellung von expressionsprofilen unter verwendung von mikroarrays
EP1694869A2 (de) 2003-11-10 2006-08-30 Investigen, Inc. Verfahren zur herstellung von nukleinsäure für einen nachweis
US20080261204A1 (en) 2004-01-23 2008-10-23 Lingvitae As Polynucleotide Ligation Reactions
CA2555377A1 (en) 2004-02-12 2005-09-01 Compass Genetics, Llc Genetic analysis by sequence-specific sorting
US20100216153A1 (en) 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
US7432055B2 (en) 2004-03-05 2008-10-07 Uchicago Argonne Llc Dual phase multiplex polymerase chain reaction
US7387887B2 (en) 2004-04-20 2008-06-17 University Of Utah Research Foundation Nucleic acid melting analysis with saturation dyes
ES2527426T3 (es) 2004-05-07 2015-01-23 Cepheid Detección combinada de agentes biológicos
US20050250147A1 (en) 2004-05-10 2005-11-10 Macevicz Stephen C Digital profiling of polynucleotide populations
CA2569745A1 (en) 2004-06-07 2005-12-22 Irm Llc Dispensing systems, software, and related methods
US20060035258A1 (en) 2004-08-06 2006-02-16 Affymetrix, Inc. Methods for identifying DNA copy number changes
US20060041385A1 (en) 2004-08-18 2006-02-23 Bauer Kenneth D Method of quantitating proteins and genes in cells using a combination of immunohistochemistry and in situ hybridization
US7713697B2 (en) 2004-08-27 2010-05-11 Gen-Probe Incorporated Methods and kits for amplifying DNA
US7803580B2 (en) 2004-09-10 2010-09-28 Human Genetic Signatures Pty. Ltd. Amplification blocker comprising intercalating nucleic acids (INA) containing intercalating pseudonucleotides (IPN)
US20060073506A1 (en) 2004-09-17 2006-04-06 Affymetrix, Inc. Methods for identifying biological samples
US7435084B2 (en) 2004-12-14 2008-10-14 Align Technology, Inc. System and methods for casting physical tooth model
CN103146808A (zh) 2004-11-03 2013-06-12 意力速分子诊断股份有限公司 均相分析物检测
EP2418018B1 (de) 2004-12-23 2013-05-22 Abbott Point of Care Inc. Verfahren zur Separation von Nukleinsäuren
US20090208936A1 (en) 2005-01-04 2009-08-20 Univeristy Of Florida Research Foundations Inc. Identification of molecular interactions and therapeutic uses thereof
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
JP5526326B2 (ja) 2005-02-10 2014-06-18 独立行政法人理化学研究所 核酸配列増幅方法
US7407757B2 (en) 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
US20060211030A1 (en) 2005-03-16 2006-09-21 Sydney Brenner Methods and compositions for assay readouts on multiple analytical platforms
EP2371453A1 (de) 2005-03-18 2011-10-05 Fluidigm Corporation Mikrofluide Vorrichtung
US20060257902A1 (en) 2005-03-25 2006-11-16 Ambion, Inc. Methods and compositions for depleting abundant RNA transcripts
US7695886B2 (en) 2005-05-19 2010-04-13 Fuji Xerox Co., Ltd. Process for producing resin particle liquid dispersion for electrostatic image developing toner, electrostatic image developing toner and production process thereof
US20060263789A1 (en) 2005-05-19 2006-11-23 Robert Kincaid Unique identifiers for indicating properties associated with entities to which they are attached, and methods for using
US8407013B2 (en) 2005-06-07 2013-03-26 Peter K. Rogan AB initio generation of single copy genomic probes
US20070065844A1 (en) 2005-06-08 2007-03-22 Massachusetts Institute Of Technology Solution-based methods for RNA expression profiling
US7796815B2 (en) 2005-06-10 2010-09-14 The Cleveland Clinic Foundation Image analysis of biological objects
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
US8486621B2 (en) 2005-08-11 2013-07-16 Cornell Research Foundation, Inc. Nucleic acid-based matrixes
JP2009508495A (ja) 2005-09-16 2009-03-05 454 ライフ サイエンシーズ コーポレイション cDNAライブラリー調製
WO2007044974A2 (en) 2005-10-12 2007-04-19 The Research Foundation Of State University Of New York Absolute pcr quantification
WO2007053692A1 (en) 2005-11-01 2007-05-10 Symyx Technologies, Inc. Liquid dispensing for high-throughput experimentation
US7375211B2 (en) 2005-11-18 2008-05-20 Kou Zhong C Method for detection and quantification of T-cell receptor Vβ repertoire
US20080070303A1 (en) 2005-11-21 2008-03-20 West Michael D Methods to accelerate the isolation of novel cell strains from pluripotent stem cells and cells obtained thereby
US7636465B2 (en) 2005-12-09 2009-12-22 Cytyc Corporation Cross-frame object reconstruction for image-based cytology applications
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
US7544473B2 (en) 2006-01-23 2009-06-09 Population Genetics Technologies Ltd. Nucleic acid analysis using sequence tokens
US8492098B2 (en) 2006-02-21 2013-07-23 The Trustees Of Tufts College Methods and arrays for target analyte detection and determination of reaction components that affect a reaction
US20080038727A1 (en) 2006-03-10 2008-02-14 Applera Corporation MicroRNA and Messenger RNA Detection on Arrays
US7880142B2 (en) 2006-04-04 2011-02-01 Sidec Technologies Ab Extended electron tomography
US20080194414A1 (en) 2006-04-24 2008-08-14 Albert Thomas J Enrichment and sequence analysis of genomic regions
WO2007136874A2 (en) 2006-05-18 2007-11-29 President And Fellows Of Harvard College Genomic library construction
US7833716B2 (en) * 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
CN101675169B (zh) 2006-06-14 2018-01-02 维里纳塔健康公司 使用样品拆分和dna标签进行稀有细胞分析
EP2077912B1 (de) 2006-08-07 2019-03-27 The President and Fellows of Harvard College Fluorkohlenstoffemulsionsstabilisierende tenside
AU2007284651B2 (en) 2006-08-09 2014-03-20 Institute For Systems Biology Organ-specific proteins and methods of their use
EP2069472A4 (de) 2006-10-05 2012-06-20 Nanopoint Inc Systeme und verfahren zur handhabung einer aktiven mikrofluidischen zelle
US8158444B2 (en) 2006-10-06 2012-04-17 Sirigen, Inc. Fluorescent methods and materials for directed biomarker signal amplification
WO2008057163A2 (en) 2006-10-09 2008-05-15 Carnegie Mellon University Preparation of functional gel particles with a dual crosslink network
CN101542298B (zh) 2006-10-18 2012-06-13 大崎电气工业株式会社 电子电度表
WO2008051928A2 (en) 2006-10-23 2008-05-02 The Salk Institute For Biological Studies Target-oriented whole genome amplification of nucliec acids
EP2518162B1 (de) 2006-11-15 2018-03-07 Biospherex LLC Mehrfach-Tags-Sequenzierung und ökogenomische Analyse
US8050476B2 (en) 2006-11-22 2011-11-01 General Electric Company Heart rate demodulation of periodic movement in ultrasound data for object identification
AU2007334393A1 (en) 2006-12-14 2008-06-26 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP2096429A4 (de) 2007-01-16 2009-12-16 Olympus Corp Vorrichtung zur analyse fluoreszierender signale und verfahren zur analyse fluoreszierender signale
WO2008109207A2 (en) 2007-01-30 2008-09-12 The Regents Of The University Of California Methods and devices for biomolecular arrays
US20080269068A1 (en) 2007-02-06 2008-10-30 President And Fellows Of Harvard College Multiplex decoding of sequence tags in barcodes
WO2008096318A2 (en) 2007-02-09 2008-08-14 Koninklijke Philips Electronics N.V. Identification system
US8003312B2 (en) 2007-02-16 2011-08-23 The Board Of Trustees Of The Leland Stanford Junior University Multiplex cellular assays using detectable cell barcodes
RU2489720C2 (ru) 2007-02-27 2013-08-10 Сентоклон Интернэшнл Аб Мультиплексная детекция опухолевых клеток с использованием панели агентов, связывающихся с внеклеточными маркерами
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
KR100882711B1 (ko) 2007-03-12 2009-02-06 성균관대학교산학협력단 사이크로박터 스피시스 hj147 균주 유래의 우라실-dna글리코실라제 및 이의 용도
JP2008256428A (ja) 2007-04-03 2008-10-23 Intec Systems Institute Inc マイクロアレイ画像のブロック位置検出方法
US20080268508A1 (en) 2007-04-30 2008-10-30 Sowlay Mohankumar R Methods and kits for negative selection of desired nucleic acid sequences
US20080274458A1 (en) 2007-05-01 2008-11-06 Latham Gary J Nucleic acid quantitation methods
US20090061513A1 (en) 2007-05-15 2009-03-05 Picovitro Ab Cell sorting and cell cultivation methods
CN101720359A (zh) 2007-06-01 2010-06-02 454生命科学公司 从多重混合物中识别个别样本的系统和方法
EP2395113A1 (de) 2007-06-29 2011-12-14 Population Genetics Technologies Ltd. Verfahren und Zusammensetzungen zur Isolierung von Nukleinsäuresequenzvarianten
ES2391906T3 (es) 2007-09-12 2012-11-30 Institut Pasteur Polinucleótido apto para un ensayo indicador basado en una sola célula para controlar los patrones de expresión génica con una resolución espacio-temporal elevada
EP2511708B1 (de) 2007-10-05 2016-09-14 Affymetrix, Inc. Assays auf der Basis hoch gebündelter Partikel
CA2740973A1 (en) * 2007-10-23 2009-04-30 Stratos Genomics Inc. High throughput nucleic acid sequencing by spacing
CA2720747A1 (en) 2008-02-21 2009-08-27 Gentel Biosciences, Inc. Substrates for multiplexed assays and uses thereof
US8687189B2 (en) 2008-03-03 2014-04-01 Ajjer, Llc Analysis of arrays by laser induced breakdown spectroscopy
US8206925B2 (en) 2008-04-14 2012-06-26 Medical Diagnostic Laboratories, Llc Splice variants of human IL-23 receptor (IL-23R) mRNA and use of a delta 9 isoform in predicting inflammatory bowel diseases
DE102008025656B4 (de) 2008-05-28 2016-07-28 Genxpro Gmbh Verfahren zur quantitativen Analyse von Nukleinsäuren, Marker dafür und deren Verwendung
US20090298709A1 (en) 2008-05-28 2009-12-03 Affymetrix, Inc. Assays for determining telomere length and repeated sequence copy number
WO2009148560A2 (en) 2008-05-30 2009-12-10 Board Of Regents, The Universtiy Of Texas System Methods and compositions for nucleic acid sequencing
WO2010021936A1 (en) 2008-08-16 2010-02-25 The Board Of Trustees Of The Leland Stanford Junior University Digital pcr calibration for high throughput sequencing
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
EP2664678B1 (de) * 2008-10-24 2014-10-08 Epicentre Technologies Corporation Transposonendenzusammensetzungen und Verfahren zur Modifizierung von Nukleinsäuren
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US8835110B2 (en) 2008-11-04 2014-09-16 The Johns Hopkins University DNA integrity assay (DIA) for cancer diagnostics, using confocal fluorescence spectroscopy
US8309306B2 (en) 2008-11-12 2012-11-13 Nodality, Inc. Detection composition
US8415161B2 (en) 2008-11-13 2013-04-09 Becton, Dickinson And Company Instrument setup system for a fluorescence analyzer
EP2350657A4 (de) 2008-11-21 2013-06-12 Univ Saint Louis Biosensor zur erkennung mehrerer epitope auf einem ziel
US20100159533A1 (en) 2008-11-24 2010-06-24 Helicos Biosciences Corporation Simplified sample preparation for rna analysis
US8492096B2 (en) 2008-12-24 2013-07-23 Boris Pasche TGFBR1 expression modifies risk for colorectal cancer
CN102333891A (zh) 2009-01-20 2012-01-25 利兰·斯坦福青年大学托管委员会 用于药靶诊断、预后和鉴别的单细胞基因表达
US20100323348A1 (en) 2009-01-31 2010-12-23 The Regents Of The University Of Colorado, A Body Corporate Methods and Compositions for Using Error-Detecting and/or Error-Correcting Barcodes in Nucleic Acid Amplification Process
US8715928B2 (en) 2009-02-13 2014-05-06 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Molecular-based method of cancer diagnosis and prognosis
CA2754446A1 (en) 2009-03-05 2010-09-10 Daiichi Sankyo Company, Limited Pyridine derivative
EP2230312A1 (de) 2009-03-19 2010-09-22 Helmholtz-Zentrum für Infektionsforschung GmbH Sondenverbindung zum Nachweisen und Isolieren von Enzymen sowie Mittel und Verfahren zu ihrer Verwendung
EP3998346A1 (de) 2009-03-30 2022-05-18 Illumina, Inc. Genexpressionsanalyse in einzelzellen
SG174617A1 (en) 2009-04-02 2011-10-28 Fluidigm Corp Multi-primer amplification method for barcoding of target nucleic acids
US9085798B2 (en) 2009-04-30 2015-07-21 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
FR2945545B1 (fr) 2009-05-14 2011-08-05 Univ Aix Marseille Ii Methode de detection d'adn procaryote extrait d'un echantillon de selles
EP2431465A4 (de) 2009-05-14 2012-10-31 Wako Pure Chem Ind Ltd Verfahren zur synthese doppelsträngiger dna entsprechend einer rna und verfahren zur dna-amplifikation
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
GB0909923D0 (en) 2009-06-09 2009-07-22 Oxford Gene Tech Ip Ltd Picowell capture devices for analysing single cells or other particles
SG177355A1 (en) 2009-06-26 2012-02-28 Sirigen Inc Signal amplified biological detection with conjugated polymers
US8330957B2 (en) 2009-06-29 2012-12-11 Hager Enviromental and Atmospheric Technologies, LLC Device and method for quantification of gases in plumes by remote sensing
CN102686728B (zh) 2009-06-29 2018-09-18 卢米耐克斯公司 具有发夹构象的嵌合引物及其使用方法
EP3029141A1 (de) 2009-08-20 2016-06-08 Population Genetics Technologies Ltd. Zusammensetzungen und verfahren für intramolekulare neuanordnung von nukleinsäuren
US9493822B2 (en) 2009-09-01 2016-11-15 Koninklijke Philips Electronics N.V. Devices and methods for microarray selection
WO2011028818A2 (en) 2009-09-01 2011-03-10 Trustees Of Boston University High throughput multichannel reader and uses thereof
US9625454B2 (en) 2009-09-04 2017-04-18 The Research Foundation For The State University Of New York Rapid and continuous analyte processing in droplet microfluidic devices
US9488656B2 (en) 2009-09-30 2016-11-08 Quest Diagnostics Investments Incorporated BCR-ABL truncation mutations
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
EP2513341B1 (de) 2010-01-19 2017-04-12 Verinata Health, Inc Identifizierung von polymorphen sequenzen in mischungen aus genomischer dna durch gesamtgenomsequenzierung
JP6073684B2 (ja) 2010-01-19 2017-02-01 シリゲン セカンド リミテッド 指向性バイオマーカーシグナル増幅のための新規の試薬
EP2848704B1 (de) 2010-01-19 2018-08-29 Verinata Health, Inc Sequenzierungsverfahren für pränatale Diagnosen
EP2529026B1 (de) 2010-01-25 2013-11-13 Rd Biosciences Inc. Selbstfaltende amplifikation einer zielnukleinsäure
EP4484577A3 (de) 2010-02-12 2025-03-26 Bio-Rad Laboratories, Inc. Digitale analytanalyse
JP5901046B2 (ja) 2010-02-19 2016-04-06 国立大学法人 千葉大学 OATP1B3mRNAの新規な選択的スプライシングバリアント
WO2011106738A2 (en) 2010-02-25 2011-09-01 Fred Hutchinson Cancer Research Center Use of tcr clonotypes as biomarkers for disease
US8951940B2 (en) 2010-04-01 2015-02-10 Illumina, Inc. Solid-phase clonal amplification and related methods
SI2556171T1 (sl) 2010-04-05 2016-03-31 Prognosys Biosciences, Inc. Prostorsko kodirane biološke analize
EP2569453B1 (de) 2010-05-14 2015-12-16 Fluidigm Corporation Nukleinsäure-isolationsverfahren
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
WO2011155833A2 (en) 2010-06-09 2011-12-15 Keygene N.V. Combinatorial sequence barcodes for high throughput screening
US20120004132A1 (en) 2010-07-02 2012-01-05 Affymetrix, Inc. Detection of Nucleic Acids and Proteins
KR101374465B1 (ko) 2010-07-07 2014-03-18 가부시키가이샤 어드밴티스트 시험 장치 및 시험 방법
EP2407242A1 (de) 2010-07-13 2012-01-18 Dublin City University Direkte Klonanalyse und Auswahltechnologie
GB201011971D0 (en) 2010-07-15 2010-09-01 Olink Ab Methods and product
US20120040843A1 (en) 2010-08-12 2012-02-16 Dublin City University Centrifugal capture system
WO2012027503A2 (en) 2010-08-24 2012-03-01 Fred Hutchinson Cancer Research Center Method of measuring adaptive immunity
EP3115468B1 (de) 2010-09-21 2018-07-25 Agilent Technologies, Inc. Erhöhung der verlässlichkeit von allel-aufrufen mit molekülzählung
EP2436766A1 (de) 2010-09-29 2012-04-04 Deutsches Krebsforschungszentrum Mittel und Verfahren für eine verbesserte Untersuchung der Proteininteraktion
WO2012042374A2 (en) 2010-10-01 2012-04-05 Anssi Jussi Nikolai Taipale Method of determining number or concentration of molecules
US20120088691A1 (en) 2010-10-08 2012-04-12 Gao Chen Highly multiplexed real-time pcr using encoded microbeads
WO2012048341A1 (en) 2010-10-08 2012-04-12 President And Fellows Of Harvard College High-throughput single cell barcoding
US20130225623A1 (en) 2010-10-27 2013-08-29 Mount Sinai School Of Medicine Methods of Treating Psychiatric or Neurological Disorders with MGLUR Antagonists
CA2819181C (en) 2010-11-29 2020-03-10 Dako Denmark A/S Methods and systems for analyzing images of specimens processed by a programmable quantitative assay
US9394511B2 (en) 2010-12-05 2016-07-19 Wenbin Jiang Rapid single cell based parallel biological cell sorter
WO2012078863A2 (en) 2010-12-09 2012-06-14 Akonni Biosystems Sample analysis system
US20140057799A1 (en) 2010-12-16 2014-02-27 Gigagen System and Methods for Massively Parallel Analysis of Nucleic Acids in Single Cells
CA2821906C (en) 2010-12-22 2020-08-25 Natera, Inc. Methods for non-invasive prenatal paternity testing
US10241075B2 (en) 2010-12-30 2019-03-26 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
WO2012099896A2 (en) 2011-01-17 2012-07-26 Life Technologies Corporation Workflow for detection of ligands using nucleic acids
WO2012103154A1 (en) 2011-01-24 2012-08-02 Nugen Technologies, Inc. Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations
US20120190021A1 (en) 2011-01-25 2012-07-26 Aria Diagnostics, Inc. Detection of genetic abnormalities
EP2670863B1 (de) 2011-01-31 2018-06-27 H. Hoffnabb-La Roche Ag Verfahren zur identifizierung mehrerer epitope in zellen
AU2012212148B8 (en) * 2011-02-02 2017-07-06 University Of Washington Through Its Center For Commercialization Massively parallel contiguity mapping
US9365897B2 (en) 2011-02-08 2016-06-14 Illumina, Inc. Selective enrichment of nucleic acids
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
CA2827948C (en) 2011-02-23 2019-07-09 Board Of Regents, University Of Texas System Use of template switching for dna synthesis
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
KR20120110431A (ko) 2011-03-29 2012-10-10 에스케이하이닉스 주식회사 반도체 메모리 장치
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
AU2012242847B2 (en) 2011-04-15 2017-01-19 The Johns Hopkins University Safe sequencing system
US8946389B2 (en) 2011-04-25 2015-02-03 University Of Washington Compositions and methods for multiplex biomarker profiling
WO2012149042A2 (en) 2011-04-25 2012-11-01 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
SG194233A1 (en) 2011-04-28 2013-12-30 Univ Leland Stanford Junior Identification of polynucleotides associated with a sample
GB201108259D0 (en) 2011-05-17 2011-06-29 Cambridge Entpr Ltd Gel beads in microfluidic droplets
EP2710172B1 (de) 2011-05-20 2017-03-29 Fluidigm Corporation Nukleinsäure-kodierungsreaktionen
US9005935B2 (en) 2011-05-23 2015-04-14 Agilent Technologies, Inc. Methods and compositions for DNA fragmentation and tagging by transposases
HRP20230443T1 (hr) 2011-05-24 2023-09-15 BioNTech SE Individualizirana cjepiva protiv raka
US9518292B2 (en) 2011-05-26 2016-12-13 Brandeis University Methods for suppression PCR
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
WO2012177639A2 (en) 2011-06-22 2012-12-27 Alnylam Pharmaceuticals, Inc. Bioprocessing and bioproduction using avian cell lines
WO2013003498A2 (en) 2011-06-27 2013-01-03 Life Technologies Corporation Acoustic cytometry methods and protocols
US9340826B2 (en) 2011-08-01 2016-05-17 Celemics, Inc. Method of preparing nucleic acid molecules
WO2013022961A1 (en) 2011-08-08 2013-02-14 3The Broad Institute Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence
JP5816486B2 (ja) 2011-08-18 2015-11-18 オリンパス株式会社 蛍光観察装置および蛍光観察システム並びに蛍光観察装置の蛍光画像処理方法
US9834766B2 (en) 2011-09-02 2017-12-05 Atreca, Inc. DNA barcodes for multiplexed sequencing
WO2013038010A2 (en) 2011-09-16 2013-03-21 Lexogen Gmbh Nucleic acid transcription method
WO2013062931A2 (en) 2011-10-24 2013-05-02 Furchak Jennifer A molecular beacon based assay for the detection of biomarkers of breast cancer metastasis
DK3467500T3 (da) 2011-11-11 2021-08-16 Eli N Glezer Cobinder-understøttede assayfremgangsmåder
US10689643B2 (en) 2011-11-22 2020-06-23 Active Motif, Inc. Targeted transposition for use in epigenetic studies
US9938524B2 (en) 2011-11-22 2018-04-10 Active Motif, Inc. Multiplex isolation of protein-associated nucleic acids
KR101337094B1 (ko) 2011-11-30 2013-12-05 삼성에스디에스 주식회사 염기 서열 정렬 장치 및 그 방법
WO2013096802A1 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Amplification primers and methods
WO2013117595A2 (en) 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support
EP3495817B1 (de) 2012-02-10 2024-10-16 Bio-Rad Laboratories, Inc. Molekulardiagnostischer screeningtest
US10202628B2 (en) 2012-02-17 2019-02-12 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
US9176031B2 (en) 2012-02-24 2015-11-03 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
WO2013130512A2 (en) 2012-02-27 2013-09-06 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
GB2513024B (en) 2012-02-27 2016-08-31 Cellular Res Inc A clonal amplification method
WO2013137737A1 (en) 2012-03-16 2013-09-19 Flexgen B.V. Methods of creating and screening of dna encoded combinatorial libraries of chemical antibodies
US9803239B2 (en) 2012-03-29 2017-10-31 Complete Genomics, Inc. Flow cells for high density array chips
EP2647426A1 (de) 2012-04-03 2013-10-09 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Replikation von verteilten Nukleinsäuremolekülen mit Aufrechterhaltung ihrer relativen Verteilung durch hybridisierungsbasierte Bindung
US9150905B2 (en) 2012-05-08 2015-10-06 Adaptive Biotechnologies Corporation Compositions and method for measuring and calibrating amplification bias in multiplexed PCR reactions
WO2013173394A2 (en) 2012-05-14 2013-11-21 Cb Biotechnologies, Inc. Method for increasing accuracy in quantitative detection of polynucleotides
CA2874343C (en) 2012-05-21 2021-11-09 Fluidigm Corporation Single-particle analysis of particle populations
US9617589B2 (en) 2012-05-25 2017-04-11 The University Of North Carolina At Chapel Hill Microfluidic devices, solid supports for reagents and related methods
AU2013273987B2 (en) 2012-06-15 2018-08-09 Adaptive Biotechnologies Corporation Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
US9708654B2 (en) 2012-06-15 2017-07-18 Board Of Regents, The University Of Texas System High throughput sequencing of multiple transcripts
JP6181751B2 (ja) 2012-06-18 2017-08-16 ニューゲン テクノロジーズ, インコーポレイテッド 望まれない核酸配列のネガティブ選択のための組成物および方法
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
EP2875173B1 (de) 2012-07-17 2017-06-28 Counsyl, Inc. System und verfahren zur erkennung von genetischen variationen
US9695416B2 (en) 2012-07-18 2017-07-04 Siemens Healthcare Diagnostics Inc. Method of normalizing biological samples
US20150247182A1 (en) 2012-07-24 2015-09-03 Adaptive Biotechnologies Corp. Single cell analysis using sequence tags
EP2877593B1 (de) 2012-07-26 2018-07-18 Illumina, Inc. Zusammensetzungen und verfahren zur amplifikation von nukleinsäuren
JP6525872B2 (ja) 2012-08-08 2019-06-05 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 細胞中の複数のエピトープを同定するためのダイナミックレンジを高めること
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4397767A3 (de) 2012-08-14 2024-07-31 10X Genomics, Inc. Mikrokapselzusammensetzungen und verfahren
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20150005199A1 (en) 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
US20140378322A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20140378349A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20140378345A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US20150005200A1 (en) 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN104884605B (zh) 2012-08-24 2018-05-18 耶鲁大学 用于高通量多重检测的系统、装置和方法
EP3252174B1 (de) 2012-10-15 2020-07-01 Life Technologies Corporation Zusammensetzungen, verfahren, systeme und kits zur zielnukleinsäureanreicherung
EP4592400A3 (de) 2012-10-17 2025-10-29 10x Genomics Sweden AB Verfahren und produkt zur optimierung einer lokalisierten oder räumlichen detektion einer genexpression in einer gewebeprobe
WO2015160439A2 (en) 2014-04-17 2015-10-22 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
GB201218909D0 (en) 2012-10-22 2012-12-05 Univ Singapore Assay for the parallel detection of biological material based on PCR
AU2013337280B2 (en) 2012-11-05 2018-11-08 Takara Bio Usa, Inc. Barcoding nucleic acids
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
WO2014108850A2 (en) 2013-01-09 2014-07-17 Yeda Research And Development Co. Ltd. High throughput transcriptome analysis
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US9562269B2 (en) 2013-01-22 2017-02-07 The Board Of Trustees Of The Leland Stanford Junior University Haplotying of HLA loci with ultra-deep shotgun sequencing
WO2014116979A1 (en) 2013-01-24 2014-07-31 Sabic Innovative Plastics Ip B.V. Microwell plate made from a polyester-polycarbonate
US10017761B2 (en) 2013-01-28 2018-07-10 Yale University Methods for preparing cDNA from low quantities of cells
ES2931314T3 (es) 2013-02-01 2022-12-28 Becton Dickinson Co Métodos y sistemas para evaluar el comportamiento de una muestra en un citómetro de flujo
US9850515B2 (en) 2013-02-08 2017-12-26 Bio-Rad Laboratories, Inc. Affinity-based partition assay for detection of target molecules
CN108753766A (zh) 2013-02-08 2018-11-06 10X基因组学有限公司 多核苷酸条形码生成
WO2014126937A1 (en) 2013-02-14 2014-08-21 The Board Of Trustees Of The Leland Stanford Junior University Suspension arrays and multiplexed assays based thereon
US9621600B2 (en) 2013-02-26 2017-04-11 PortAura Group Method and system for providing recommendations using location information
US20140274811A1 (en) 2013-03-14 2014-09-18 Lyle J. Arnold Methods for Amplifying a Complete Genome or Transcriptome
US9255265B2 (en) 2013-03-15 2016-02-09 Illumina, Inc. Methods for producing stranded cDNA libraries
US10119134B2 (en) 2013-03-15 2018-11-06 Abvitro Llc Single cell bar-coding for antibody discovery
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
WO2014145458A1 (en) 2013-03-15 2014-09-18 Arizona Board Of Regents On Behalf Of Arizona State University Nucleic acid-tagged compositions and methods for multiplexed protein-protein interaction profiling
CA2905517C (en) 2013-03-15 2024-11-12 Lineage Biosciences, Inc. METHODS AND COMPOSITIONS FOR LABELING AND ANALYZING SAMPLES
WO2014165762A1 (en) 2013-04-05 2014-10-09 Raindance Technologies, Inc. Rare cell analysis after negative selection
AU2014256903B2 (en) 2013-04-25 2020-09-24 Firefly Bioworks, Inc. Multiplexed analysis of target nucleic acids
WO2014179735A1 (en) 2013-05-03 2014-11-06 Cambrian Genomics, Inc. Method and apparatus for producing sequence verified dna
EP3470530B1 (de) 2013-05-23 2020-11-25 The Board of Trustees of the Leland Stanford Junior University Transposition in natives chromatin zur analyse des chromatins
EP2805769A1 (de) 2013-05-24 2014-11-26 European Molecular Biology Laboratory Verfahren zur nanoskaligen Einzelzellenanalyse
ES2754177T3 (es) 2013-06-12 2020-04-16 Massachusetts Gen Hospital Métodos para la detección multiplexada de moléculas diana y sus usos
WO2014201273A1 (en) 2013-06-12 2014-12-18 The Broad Institute, Inc. High-throughput rna-seq
US20150011397A1 (en) 2013-06-17 2015-01-08 Kim Lewis Methods for quantitative determination of multiple proteins in complex mixtures
EP4219745B1 (de) 2013-06-25 2025-09-03 Prognosys Biosciences, Inc. Räumlich codierte biologische assays mit einer mikrofluidischen vorrichtung
JP6563912B2 (ja) 2013-06-27 2019-08-21 テンエックス・ジェノミクス・インコーポレイテッド サンプル処理のための組成物及び方法
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
US20160153973A1 (en) 2013-07-09 2016-06-02 Lucas David Smith Device and method of rapid linker mediated label-based immunoassays
EP3027773B1 (de) 2013-07-30 2020-03-25 President and Fellows of Harvard College Quantitative bildgebung auf dna-basis und hochauflösende bildgebung
GB2546833B (en) * 2013-08-28 2018-04-18 Cellular Res Inc Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
SG10201908167YA (en) 2013-09-04 2019-10-30 Fluidigm Corp Proximity assays for detecting nucleic acids and proteins in a single cell
DK3052658T3 (da) 2013-09-30 2021-06-07 Vesicode Ab Fremgangsmåder til profilering af molekylkomplekser ved anvendelse af proksimitetsafhængig stregkodning
GB201317301D0 (en) 2013-09-30 2013-11-13 Linnarsson Sten Method for capturing and encoding nucleic acid from a plurality of single cells
US9582877B2 (en) 2013-10-07 2017-02-28 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
HUE045954T2 (hu) 2013-10-17 2020-01-28 Illumina Inc Eljárások és készítmények nukleinsavkönyvtárak elõállítására
US9709479B2 (en) 2013-10-25 2017-07-18 Massachusetts Institute Of Technology Method and apparatus for tracking cell identity
WO2015061844A1 (en) 2013-10-30 2015-05-07 The University Of Sydney Assay to stratify cancer patients
US10288608B2 (en) 2013-11-08 2019-05-14 Prognosys Biosciences, Inc. Polynucleotide conjugates and methods for analyte detection
US9526450B2 (en) 2013-11-27 2016-12-27 Elwha Llc Devices and methods for profiling microbiota of skin
PT3089822T (pt) 2013-12-30 2022-05-06 Atreca Inc Análise de ácidos nucleicos associados com células isoladas usando códigos de barras de ácido nucleico
CN106661612A (zh) 2014-01-27 2017-05-10 通用医疗公司 制备用于测序的核酸的方法
EP3102678A2 (de) 2014-02-03 2016-12-14 Integrated DNA Technologies Inc. Verfahren zur erfassung und/oder entfernung von hochabundanten rnas aus einer heterogenen rna-probe
WO2015128272A2 (en) 2014-02-26 2015-09-03 Ventana Medical Systems, Inc. Photo-selective method for biological sample analysis field
ES2741740T3 (es) 2014-03-05 2020-02-12 Adaptive Biotechnologies Corp Métodos que usan moléculas sintéticas que contienen segmentos de nucleótidos aleatorios
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
US10975371B2 (en) 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
US20170044525A1 (en) 2014-04-29 2017-02-16 Illumina, Inc. Multiplexed single cell gene expression analysis using template switch and tagmentation
PH12016502542B1 (en) 2014-05-19 2024-01-17 Univ Rice William M Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
EP3155426B1 (de) 2014-06-13 2023-07-19 Immudex ApS Allgemeiner nachweis und isolierung spezifischer zellen durch binden markierter moleküle
US11155809B2 (en) 2014-06-24 2021-10-26 Bio-Rad Laboratories, Inc. Digital PCR barcoding
AU2015279617A1 (en) 2014-06-26 2017-01-12 10X Genomics, Inc. Analysis of nucleic acid sequences
EP3161160B1 (de) 2014-06-26 2021-10-13 10X Genomics, Inc. Verfahren zur analyse von nukleinsäuren aus einzelzellen oder zellpopulationen
WO2016010856A1 (en) 2014-07-15 2016-01-21 Qiagen Sciences, Llc Semi-random barcodes for nucleic acid analysis
US11408024B2 (en) 2014-09-10 2022-08-09 Molecular Loop Biosciences, Inc. Methods for selectively suppressing non-target sequences
MX378730B (es) 2014-09-15 2025-03-11 Abvitro Llc Secuenciacion de bibliotecas de nucleotidos de alto rendimiento
US9529672B2 (en) 2014-09-25 2016-12-27 Everspin Technologies Inc. ECC word configuration for system-level ECC compatibility
LT3207134T (lt) * 2014-10-17 2019-09-10 Illumina Cambridge Limited Sukibimą išsauganti transpozicija
DK3215618T3 (da) 2014-11-05 2019-10-14 Illumina Inc Transposasesammensætninger til reduktion af insertionsbias
CN107110849B (zh) 2014-12-19 2019-07-26 豪夫迈·罗氏有限公司 在选定的细胞亚群中鉴别多个表位的方法
CN107407691B (zh) * 2015-01-22 2021-07-27 贝克顿迪金森公司 用于单细胞中核酸靶标的分子条码化的装置和系统
WO2016118719A1 (en) 2015-01-23 2016-07-28 Qiagen Sciences, Llc High multiplex pcr with molecular barcoding
WO2016126871A2 (en) 2015-02-04 2016-08-11 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
EP3256606B1 (de) 2015-02-09 2019-05-22 10X Genomics, Inc. Systeme und verfahren zur bestimmung struktureller varianten
ES2975332T3 (es) 2015-02-19 2024-07-04 Becton Dickinson Co Análisis unicelular de alto rendimiento que combina información proteómica y genómica
EP3262192B1 (de) 2015-02-27 2020-09-16 Becton, Dickinson and Company Räumlich adressierbare molekulare barcodierung
CN107208157B (zh) 2015-02-27 2022-04-05 贝克顿迪金森公司 用于条形编码核酸以用于测序的方法和组合物
CA2976681A1 (en) 2015-02-27 2016-09-01 Fluidigm Corporation Single-cell nucleic acids for high-throughput studies
EP3268462B1 (de) 2015-03-11 2021-08-11 The Broad Institute, Inc. Genotyp- und phänotypkupplung
US20160266094A1 (en) 2015-03-13 2016-09-15 University Of Iowa Research Foundation Cellular barcode
WO2016149418A1 (en) 2015-03-18 2016-09-22 Cellular Research, Inc. Methods and compositions for labeling targets and haplotype phasing
US10301660B2 (en) 2015-03-30 2019-05-28 Takara Bio Usa, Inc. Methods and compositions for repair of DNA ends by multiple enzymatic activities
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
WO2016168825A1 (en) 2015-04-17 2016-10-20 Centrillion Technology Holdings Corporation Methods for performing spatial profiling of biological molecules
EP3286326B1 (de) 2015-04-23 2025-01-22 Becton, Dickinson and Company Verfahren zur gesamttranskriptomamplifikation
US9618871B2 (en) 2015-04-28 2017-04-11 Kyocera Document Solutions Inc. Image forming apparatus
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
AU2016268089B2 (en) 2015-05-22 2021-09-23 Sigma-Aldrich Co. Llc Methods for next generation genome walking and related compositions and kits
WO2016190795A1 (en) 2015-05-28 2016-12-01 Kaarel Krjutskov Blocking oligonucleotides
WO2016196229A1 (en) 2015-06-01 2016-12-08 Cellular Research, Inc. Methods for rna quantification
MX2018000729A (es) 2015-07-17 2018-09-06 Harvard College Métodos para amplificar las secuencias de ácido nucleico.
JP6697070B2 (ja) 2015-08-12 2020-05-20 ツェーエーエムエム・フォルシュングスツェントルム・フュア・モレクラーレ・メディツィン・ゲーエムベーハー 核酸の研究方法
KR20180036712A (ko) 2015-08-24 2018-04-09 키아겐 게엠베하 Rna-서열결정 라이브러리를 생성하는 방법
SG10201912283RA (en) 2015-08-28 2020-02-27 Illumina Inc Nucleic acid sequence analysis from single cells
EP3347465B1 (de) 2015-09-11 2019-06-26 Cellular Research, Inc. Verfahren und zusammensetzung zur standardisierung von nukleinsäure bibliothek
JP6869233B2 (ja) 2015-09-24 2021-05-12 アブビトロ, エルエルシー 親和性−オリゴヌクレオチドコンジュゲートおよびその使用
US11092607B2 (en) 2015-10-28 2021-08-17 The Board Institute, Inc. Multiplex analysis of single cell constituents
SG11201803646YA (en) 2015-11-04 2018-05-30 Atreca Inc Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
WO2017087873A1 (en) 2015-11-18 2017-05-26 Wafergen, Inc. Systems and methods for pooling samples from multi-well devices
WO2017096239A1 (en) 2015-12-04 2017-06-08 St. Jude Children's Research Hospital, Inc. Cloning and expression system for t-cell receptors
WO2017117358A1 (en) 2015-12-30 2017-07-06 Bio-Rad Laboratories, Inc. Digital protein quantification
US20170205404A1 (en) 2016-01-19 2017-07-20 General Electric Company Multifunctional beads and methods of use for capturing rare cells
WO2017139690A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Cell population analysis using single nucleotide polymorphisms from single cell transcriptomes
WO2017156336A1 (en) 2016-03-10 2017-09-14 The Board Of Trustees Of The Leland Stanford Junior University Transposase-mediated imaging of the accessible genome
US12060412B2 (en) 2016-03-21 2024-08-13 The Broad Institute, Inc. Methods for determining spatial and temporal gene expression dynamics in single cells
CN109312331B (zh) 2016-04-01 2022-08-02 贝勒医学院 全转录组扩增的方法
KR102379048B1 (ko) 2016-05-02 2022-03-28 엔코디아, 인코포레이티드 암호화 핵산을 사용한 거대분자 분석
ES2956757T3 (es) 2016-05-02 2023-12-27 Becton Dickinson Co Codificación con códigos de barras moleculares precisa
WO2017196768A1 (en) 2016-05-09 2017-11-16 President And Fellows Of Harvard College Self-targeting guide rnas in crispr system
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
ES2979395T3 (es) 2016-05-26 2024-09-25 Becton Dickinson Co Métodos de ajuste del recuento de etiquetas moleculares
US10240196B2 (en) 2016-05-27 2019-03-26 Agilent Technologies, Inc. Transposase-random priming DNA sample preparation
TWI600309B (zh) 2016-05-28 2017-09-21 Hon Hai Prec Ind Co Ltd 角度調整機構
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
LT3263715T (lt) 2016-06-28 2020-06-25 Hifibio Pavienių ląstelių transkriptomo analizės būdas
US20180016632A1 (en) 2016-07-12 2018-01-18 Kapa Biosystems, Inc. System and method for transposase-mediated amplicon sequencing
EP3485037B1 (de) 2016-07-18 2022-02-09 F. Hoffmann-La Roche AG Asymmetrische schablonen und asymmetrisches verfahren zur nukleinsäuresequenzierung
US10809266B2 (en) 2016-07-22 2020-10-20 Verily Life Sciences Llc Quantitative massively parallel proteomics
DK3488002T3 (da) 2016-07-22 2021-06-21 Univ Oregon Health & Science Enkeltcelle-helgenombiblioteker og kombinatoriske indekseringsfremgangsmåder til fremstilling deraf
WO2018020489A1 (en) 2016-07-24 2018-02-01 Yeda Research And Development Co. Ltd. Methods and kits for analyzing dna binding moieties attached to dna
WO2018022809A1 (en) 2016-07-27 2018-02-01 The Board Of Trustees Of The Leland Stanford Junior University Highly-multiplexed fluorescent imaging
US20180037942A1 (en) 2016-08-03 2018-02-08 Cellular Research, Inc. Enzyme-independent molecular indexing
CN109804068A (zh) * 2016-08-10 2019-05-24 哈佛学院董事及会员团体 从头组装条码化的基因组dna片段的方法
CN109791157B (zh) 2016-09-26 2022-06-07 贝克顿迪金森公司 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
JP6768815B2 (ja) 2016-09-29 2020-10-14 富士フイルム株式会社 マルチプレックスpcrに供するプライマーの設計方法
EP3521425A4 (de) 2016-09-29 2019-10-23 FUJIFILM Corporation Verfahren zum entwerfen von primern für multiplex-pcr
EP3519612B1 (de) 2016-10-01 2022-04-06 Berkeley Lights, Inc. Dna-barcode-zusammensetzungen und verfahren zur in-situ-identifizierung in einer mikrofluidischen vorrichtung
CN109983126A (zh) 2016-10-19 2019-07-05 10X基因组学有限公司 用于条形码化单个细胞或细胞群的核酸分子的方法和系统
WO2018090298A2 (en) 2016-11-17 2018-05-24 Genomicare Biotechnology (Shanghai) Co. Ltd. Systems and methods for monitoring lifelong tumor evolution
EP3551768B1 (de) 2016-12-12 2024-03-06 Grail, LLC Verfahren zur markierung und amplifizierung von rna-template-molekülen zur herstellung von sequenzierungsbibliotheken
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
MX2019007444A (es) 2016-12-22 2019-08-16 Guardant Health Inc Metodos y sistemas para analisis de moleculas de acido nucleico.
US20190177800A1 (en) 2017-12-08 2019-06-13 10X Genomics, Inc. Methods and compositions for labeling cells
GB201622222D0 (en) 2016-12-23 2017-02-08 Cs Genetics Ltd Reagents and methods for molecular barcoding of nucleic acids of single cells
WO2018132635A1 (en) 2017-01-12 2018-07-19 Massachusetts Institute Of Technology Methods for analyzing t cell receptors and b cell receptors
US20180208975A1 (en) 2017-01-20 2018-07-26 Merck Sharp & Dohme Corp. Assay for simultaneous genomic and proteomic analysis
EP4029939B1 (de) 2017-01-30 2023-06-28 10X Genomics, Inc. Verfahren und systeme für eine einzelzellbarcodierung auf tröpfchenbasis
WO2018144240A1 (en) 2017-02-01 2018-08-09 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
WO2018144813A1 (en) 2017-02-02 2018-08-09 New York Genome Center Methods and compositions for identifying or quantifying targets in a biological sample
EP3583212A4 (de) 2017-02-16 2021-01-13 Takara Bio USA, Inc. Verfahren zur herstellung von nukleinsäurebibliotheken und zusammensetzungen und kits zur durchführung davon
KR102607830B1 (ko) * 2017-02-21 2023-12-01 일루미나, 인코포레이티드 링커를 갖는 고정된 트랜스포좀을 사용한 태그먼트화
US20200063213A1 (en) 2017-03-08 2020-02-27 President And Fellows Of Harvard College Methods of Amplifying DNA to Maintain Methylation Status
WO2018170515A1 (en) * 2017-03-17 2018-09-20 The Broad Institute, Inc. Methods for identifying and modulating co-occurant cellular phenotypes
WO2018174827A1 (en) 2017-03-24 2018-09-27 National University Of Singapore Methods for multiplex detection of molecules
CN110446789A (zh) 2017-03-24 2019-11-12 赛卢拉研究公司 用于多重态确定的合成多重态
US10914729B2 (en) 2017-05-22 2021-02-09 The Trustees Of Princeton University Methods for detecting protein binding sequences and tagging nucleic acids
EP3631054A4 (de) 2017-05-23 2021-03-03 President and Fellows of Harvard College Multiplex-enden-markierungsamplifikation von nukleinsäuren
EP3631001B1 (de) 2017-05-23 2025-04-23 Centrillion Technology Holdings Corporation Verfahren zur durchführung von räumlicher profilierung biologischer moleküle
WO2018218226A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP3445876B1 (de) 2017-05-26 2023-07-05 10X Genomics, Inc. Einzelzellenanalyse von transposasezugänglichem chromatin
WO2018218222A1 (en) 2017-05-26 2018-11-29 Goldfless Stephen Jacob High-throughput polynucleotide library sequencing and transcriptome analysis
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
WO2018222548A1 (en) 2017-05-29 2018-12-06 President And Fellows Of Harvard College A method of amplifying single cell transcriptome
AU2018281745B2 (en) 2017-06-05 2022-05-19 Becton, Dickinson And Company Sample indexing for single cells
WO2019055852A1 (en) 2017-09-15 2019-03-21 Apton Biosystems, Inc. PROFILING OF HETEROGENEOUS UNICELLULAR ORGANISMS USING MOLECULAR BARCODE CODING
EP4379727A1 (de) 2017-09-25 2024-06-05 Becton, Dickinson and Company Fehlerkorrektur für immunrezeptor-barcode
ES2974557T3 (es) 2017-09-25 2024-06-27 Fred Hutchinson Cancer Center Perfiles in situ de alta eficiencia dirigidos a todo el genoma
WO2019076768A1 (en) 2017-10-16 2019-04-25 Tervisetehnoloogiate Arenduskeskus As METHOD AND KIT FOR PREPARING DNA BANK
WO2019084046A1 (en) 2017-10-23 2019-05-02 The Broad Institute, Inc. ENRICHING SINGLE CELL CELLULAR CONSTITUENT OF BARCODE SEQUENCING LIBRARY
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
CN118186055A (zh) 2017-11-02 2024-06-14 生物辐射实验室股份有限公司 基于转座酶的基因组分析
WO2019094984A1 (en) * 2017-11-13 2019-05-16 The Broad Institute, Inc. Methods for determining spatial and temporal gene expression dynamics during adult neurogenesis in single cells
WO2019099906A1 (en) 2017-11-16 2019-05-23 The Regents Of The University Of Colorado, A Body Corporate Methods to measure functional heterogeneity among single cells
CA3084439A1 (en) 2017-12-07 2019-06-13 Massachusetts Institute Of Technology Single cell analyses
WO2019113499A1 (en) 2017-12-07 2019-06-13 The Broad Institute, Inc. High-throughput methods for identifying gene interactions and networks
WO2019113506A1 (en) 2017-12-07 2019-06-13 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
EP3919626B1 (de) 2017-12-08 2024-07-10 10X Genomics, Inc. Verfahren und zusammensetzungen zur markierung von zellen
WO2019118355A1 (en) 2017-12-12 2019-06-20 10X Genomics, Inc. Systems and methods for single cell processing
US11946095B2 (en) * 2017-12-19 2024-04-02 Becton, Dickinson And Company Particles associated with oligonucleotides
CN111712579B (zh) 2017-12-22 2024-10-15 10X基因组学有限公司 用于处理来自一个或多个细胞的核酸分子的系统和方法
JP7164125B2 (ja) 2018-01-05 2022-11-01 ビリオントゥーワン,インコーポレイテッド シーケンシングベースのアッセイの妥当性を確保するための品質管理鋳型
CN119020348A (zh) * 2018-02-05 2024-11-26 斯坦福大学托管董事会 用于单细胞和集合细胞的多重测量的系统和方法
EP3752832A1 (de) 2018-02-12 2020-12-23 10X Genomics, Inc. Verfahren zur charakterisierung mehrerer analyten aus einzelnen zellen oder zellpopulationen
WO2019178164A1 (en) 2018-03-12 2019-09-19 Silicon Valley Scientific, Inc. Method and apparatus for processing tissue and other samples encoding cellular spatial position information
WO2019204560A1 (en) * 2018-04-18 2019-10-24 The Regents Of The University Of California Method to connect chromatin accessibility and transcriptome
WO2019210049A1 (en) * 2018-04-27 2019-10-31 X Gen Us Co. Methods and compositions for preparing polynucleotides
ES2945191T3 (es) 2018-05-03 2023-06-29 Becton Dickinson Co Análisis de muestras multiómicas de alto rendimiento
EP3788170B1 (de) 2018-05-03 2025-01-01 Becton, Dickinson and Company Molekulare barcodierung auf gegenüberliegenden transkriptenden
CN110499251A (zh) 2018-05-17 2019-11-26 中国科学院大连化学物理研究所 高通量多参数的单细胞源性细胞外囊泡分析芯片及应用
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
GB201810571D0 (en) 2018-06-27 2018-08-15 Cs Genetics Ltd Reagents and methods for the analysis of circulating microparticles
WO2020009665A1 (en) 2018-07-06 2020-01-09 Agency For Science, Technology And Research Method for single-cell transcriptome and accessible regions sequencing
DK3821011T5 (da) 2018-07-11 2024-08-19 Cygnus Biosciences Beijing Co Ltd Transposom-aktiveret dna/rna-sekventering (ted rna-seq)
CN112840024A (zh) 2018-08-03 2021-05-25 贝克顿迪金森公司 单细胞中的核条形码化和捕获
CA3108770A1 (en) 2018-08-08 2020-02-13 Vanderbilt University Systems and methods for simultaneous detection of antigens and antigen specific antibodies
EP4242324B1 (de) 2018-08-17 2025-09-24 Becton, Dickinson and Company Aptamer-strichcodierung
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
EP3844308A1 (de) 2018-08-28 2021-07-07 10X Genomics, Inc. Auflösung von räumlichen anordnungen
ES3000357T3 (en) 2018-08-28 2025-02-28 10X Genomics Inc Methods for generating spatially barcoded arrays
EP3844299A1 (de) 2018-08-28 2021-07-07 Becton Dickinson and Company Probenmultiplexierung mit kohlenhydratbindenden und membrandurchlässigen reagenzien
EP4471156A3 (de) 2018-10-01 2025-02-26 Becton, Dickinson and Company Bestimmung von 5'-transkriptsequenzen
WO2020097315A1 (en) 2018-11-08 2020-05-14 Cellular Research, Inc. Whole transcriptome analysis of single cells using random priming
CN113195717A (zh) 2018-12-13 2021-07-30 贝克顿迪金森公司 单细胞全转录组分析中的选择性延伸
US20220056534A1 (en) 2018-12-17 2022-02-24 Natera, Inc. Methods for analysis of circulating cells
US12297565B2 (en) 2018-12-17 2025-05-13 Illumina, Inc. Methods and means for preparing a library for sequencing
WO2020154247A1 (en) 2019-01-23 2020-07-30 Cellular Research, Inc. Oligonucleotides associated with antibodies
EP3918090A4 (de) 2019-01-28 2022-10-26 Becton, Dickinson and Company Reagenzien zur bindung von oligonukleotidhaltigen zellulären komponenten und verfahren zur verwendung davon
SG11202108788TA (en) 2019-02-12 2021-09-29 10X Genomics Inc Methods for processing nucleic acid molecules
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020167830A1 (en) 2019-02-12 2020-08-20 Becton, Dickinson And Company Determining expressions of transcript variants and polyadenylation sites
EP3924506A1 (de) 2019-02-14 2021-12-22 Becton Dickinson and Company Hybride gezielte und gesamttranskriptomamplifikation
CN114174531A (zh) 2019-02-28 2022-03-11 10X基因组学有限公司 用空间条码化寡核苷酸阵列对生物分析物进行概况分析
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
US20220218847A1 (en) 2019-04-23 2022-07-14 The Broad Institute, Inc. Compositions and methods characterizing metastasis
SG10201904897UA (en) 2019-05-30 2020-12-30 Nat Univ Singapore Method, structures and system for nucleic acid sequence topology assembly for multiplexed profiling of proteins.
EP3980537A4 (de) 2019-06-04 2023-11-22 Universal Sequencing Technology Corporation Verfahren zur barcodierung von nukleinsäuren zur detektion und sequenzierung
US20220267759A1 (en) * 2019-07-12 2022-08-25 New York Genome Center, Inc. Methods and compositions for scalable pooled rna screens with single cell chromatin accessibility profiling
CN114051534B (zh) 2019-07-22 2025-02-21 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
CN110498858B (zh) 2019-07-26 2024-01-23 深圳市达科为生物工程有限公司 一种动态检测单细胞外泌蛋白分泌情况的方法
US20210039582A1 (en) 2019-08-07 2021-02-11 ZF Passive Safety Systems US Inc. Airbag package with improved packaging
EP4407041A3 (de) 2019-11-08 2024-09-25 Becton Dickinson and Company Verwendung von random-rpiming zur gewinnung von v(d)j-informationen in voller länge für immunrepertoiresequenzierung
CN115210760B (zh) 2019-11-21 2023-08-01 10X基因组学有限公司 分析物的空间分析
WO2021113353A1 (en) 2019-12-02 2021-06-10 Beth Israel Deaconess Medical Center, Inc. Methods for dual dna/protein tagging of open chromatin
EP4628596A2 (de) 2020-01-10 2025-10-08 10x Genomics, Inc. Verfahren zur bestimmung einer position einer zielnukleinsäure in einer biologischen probe
WO2021146219A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Cell capture using du-containing oligonucleotides
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
US20210222244A1 (en) 2020-01-17 2021-07-22 Becton, Dickinson And Company Methods and compositions for single cell secretomics
EP4471155A3 (de) 2020-01-29 2024-12-18 Becton, Dickinson and Company Strichcodierte bohrlöcher zur räumlichen abbildung einzelner zellen durch sequenzierung
US20210238661A1 (en) 2020-01-31 2021-08-05 Becton, Dickinson And Company Mesophilic dna polymerase extension blockers
US20210246492A1 (en) 2020-02-12 2021-08-12 Becton, Dickinson And Company Intracellular abseq
CN115398547A (zh) 2020-02-13 2022-11-25 10X基因组学有限公司 使用基因表达和染色质可接近性表征细胞的方法
EP4106769A4 (de) 2020-02-17 2024-03-27 Universal Sequencing Technology Corporation Verfahren zur barcodierung von nukleinsäuren zur detektion und sequenzierung
CN116157533A (zh) 2020-02-21 2023-05-23 10X基因组学有限公司 使用杂交方法捕获遗传靶标
CN115151810A (zh) 2020-02-25 2022-10-04 贝克顿迪金森公司 实现使用单细胞样品作为单色补偿对照的双特异性探针
WO2021178199A1 (en) 2020-03-05 2021-09-10 BioLegend, Inc. Methods and compositions for detecting targets
US20210332354A1 (en) 2020-04-15 2021-10-28 10X Genomics, Inc. Systems and methods for identifying differential accessibility of gene regulatory elements at single cell resolution
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司(Us) 用于免疫组库谱分析的引物
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
JP2023531463A (ja) 2020-06-18 2023-07-24 ナノストリング テクノロジーズ,インコーポレイティド 酵素的核酸伸長を用いたin situ単一細胞解析のための組成物及び方法
US20230227813A1 (en) 2020-06-23 2023-07-20 Ludwig Institute For Cancer Research Ltd Parallel analysis of individual cells for rna expression and dna from targeted tagmentation by sequencing
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US20220010362A1 (en) 2020-07-13 2022-01-13 Becton, Dickinson And Company cDNA SPIKE-IN CONTROL FOR SINGLE CELL ANALYSIS
US20230227809A1 (en) 2020-07-29 2023-07-20 The Jackson Laboratory Multiplex Chromatin Interaction Analysis with Single-Cell Chia-Drop
US12391940B2 (en) 2020-07-31 2025-08-19 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
JP2023539822A (ja) 2020-08-20 2023-09-20 ベクトン・ディキンソン・アンド・カンパニー 血液培養物から細菌を単離するための血液細胞溶解薬
WO2022056309A1 (en) 2020-09-11 2022-03-17 Fred Hutchinson Cancer Research Center Improved high efficiency targeted in situ genome-wide profiling
CN116964219A (zh) 2020-10-09 2023-10-27 10X基因组学有限公司 用于分析抗原结合分子的方法和组合物
US20240052338A1 (en) 2020-11-02 2024-02-15 Duke University Compositions for and methods of co-analyzing chromatin structure and function along with transcription output
WO2022108946A1 (en) 2020-11-17 2022-05-27 Becton, Dickinson And Company Combined analysis of cell-free nucleic acids and single cells for oncology diagnostics
WO2022109339A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Use of dextramer in single cell analysis
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
JP2023551072A (ja) 2020-11-25 2023-12-06 アリダ・バイオサイエンシーズ・インコーポレイテッド Rnaおよびdna修飾の多重プロファイリング
CN116569042A (zh) 2020-12-09 2023-08-08 贝克顿迪金森公司 多重化的单细胞免疫测定
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
WO2022143221A1 (zh) 2020-12-31 2022-07-07 中国科学院北京基因组研究所(国家生物信息中心) 用于标记核酸分子的方法和试剂盒
JP2024502618A (ja) 2021-01-08 2024-01-22 セラノーム, インコーポレイテッド 生体試料を分析するためのデバイスおよび方法
WO2022256324A1 (en) 2021-06-01 2022-12-08 10X Genomics, Inc. Methods and compositions for analyte detection and probe resolution
WO2023034739A1 (en) 2021-08-30 2023-03-09 Becton, Dickinson And Company Template switch oligonucleotide (tso) for mrna 5' analysis
CN118510907A (zh) 2021-08-31 2024-08-16 贝克顿迪金森公司 诱饵多核苷酸在单细胞多组学中的应用
EP4396581A1 (de) 2021-08-31 2024-07-10 Becton, Dickinson and Company Multiplexte proteomanalyse unter verwendung von oligonukleotidkonjugierten antikörpern
CN117897498A (zh) 2021-08-31 2024-04-16 贝克顿迪金森公司 来自固定的细胞的rna保存及回收
CN117916389A (zh) 2021-09-01 2024-04-19 贝克顿迪金森公司 使用原位逆转录的空间多组学
EP4399327B1 (de) 2021-09-08 2025-10-29 Becton, Dickinson and Company Nicht-sequenzierendes pcr-basiertes verfahren zum nachweis von antikörperkonjugierten oligonukleotiden

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016061517A2 (en) * 2014-10-17 2016-04-21 Illumina Cambridge Limited Contiguity preserving transposition

Also Published As

Publication number Publication date
CN112272710A (zh) 2021-01-26
EP3788171A1 (de) 2021-03-10
US12421548B2 (en) 2025-09-23
US11773441B2 (en) 2023-10-03
US20230295723A1 (en) 2023-09-21
US20190338357A1 (en) 2019-11-07
US12421547B2 (en) 2025-09-23
EP3788171B1 (de) 2023-04-05
JP2021522797A (ja) 2021-09-02
CA3097976A1 (en) 2019-11-07
AU2019262048A1 (en) 2020-11-12
EP4234717A2 (de) 2023-08-30
ES2945191T3 (es) 2023-06-29
JP7407128B2 (ja) 2023-12-28
WO2019213294A1 (en) 2019-11-07
EP4234717A3 (de) 2023-11-01
US20230295724A1 (en) 2023-09-21
JP2024037941A (ja) 2024-03-19

Similar Documents

Publication Publication Date Title
US12421548B2 (en) High throughput multiomics sample analysis
US12391940B2 (en) Single cell assay for transposase-accessible chromatin
US20220348904A1 (en) Molecular barcoding on opposite transcript ends
US20230109336A1 (en) Selective extension in single cell whole transcriptome analysis
US11932901B2 (en) Target enrichment using nucleic acid probes for scRNAseq
US20240376465A1 (en) Hybrid targeted and whole transcriptome amplification
US20220010362A1 (en) cDNA SPIKE-IN CONTROL FOR SINGLE CELL ANALYSIS
EP4612324A1 (de) Anwendung für einen peptidnukleinsäureblocker
WO2024097718A1 (en) Polymerase mediated end modification of abseq