LT3263715T - Pavienių ląstelių transkriptomo analizės būdas - Google Patents
Pavienių ląstelių transkriptomo analizės būdasInfo
- Publication number
- LT3263715T LT3263715T LTEP16305783.9T LT16305783T LT3263715T LT 3263715 T LT3263715 T LT 3263715T LT 16305783 T LT16305783 T LT 16305783T LT 3263715 T LT3263715 T LT 3263715T
- Authority
- LT
- Lithuania
- Prior art keywords
- single cells
- transcriptome analysis
- transcriptome
- analysis
- cells
- Prior art date
Links
- 238000000034 method Methods 0.000 title 1
- 238000011222 transcriptome analysis Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP16305783.9A EP3263715B1 (en) | 2016-06-28 | 2016-06-28 | Method for transcriptome analysis of single cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| LT3263715T true LT3263715T (lt) | 2020-06-25 |
Family
ID=56404045
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LTEP16305783.9T LT3263715T (lt) | 2016-06-28 | 2016-06-28 | Pavienių ląstelių transkriptomo analizės būdas |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20190345537A1 (lt) |
| EP (3) | EP3686287A1 (lt) |
| JP (1) | JP7016800B2 (lt) |
| CN (1) | CN108603220A (lt) |
| AU (1) | AU2016367200B2 (lt) |
| CA (1) | CA3007526A1 (lt) |
| LT (1) | LT3263715T (lt) |
| WO (1) | WO2017097939A1 (lt) |
Families Citing this family (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| GB2513024B (en) | 2012-02-27 | 2016-08-31 | Cellular Res Inc | A clonal amplification method |
| GB2546833B (en) | 2013-08-28 | 2018-04-18 | Cellular Res Inc | Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels |
| EP3262192B1 (en) | 2015-02-27 | 2020-09-16 | Becton, Dickinson and Company | Spatially addressable molecular barcoding |
| WO2016160844A2 (en) | 2015-03-30 | 2016-10-06 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
| EP3286326B1 (en) | 2015-04-23 | 2025-01-22 | Becton, Dickinson and Company | Method for whole transcriptome amplification |
| EP3347465B1 (en) | 2015-09-11 | 2019-06-26 | Cellular Research, Inc. | Methods and compositions for nucleic acid library normalization |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| CN109791157B (zh) | 2016-09-26 | 2022-06-07 | 贝克顿迪金森公司 | 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达 |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| WO2018144240A1 (en) | 2017-02-01 | 2018-08-09 | Cellular Research, Inc. | Selective amplification using blocking oligonucleotides |
| AU2018281745B2 (en) | 2017-06-05 | 2022-05-19 | Becton, Dickinson And Company | Sample indexing for single cells |
| US20210032693A1 (en) * | 2017-08-10 | 2021-02-04 | Rootpath Genomics, Inc. | Improved Method to Analyze Nucleic Acid Contents from Multiple Biological Particles |
| EP3788170B1 (en) | 2018-05-03 | 2025-01-01 | Becton, Dickinson and Company | Molecular barcoding on opposite transcript ends |
| ES2945191T3 (es) | 2018-05-03 | 2023-06-29 | Becton Dickinson Co | Análisis de muestras multiómicas de alto rendimiento |
| US11254975B2 (en) * | 2018-05-24 | 2022-02-22 | National Center For Child Health And Development | Method of amplifying a polynucleotide of interest |
| CN110684829A (zh) * | 2018-07-05 | 2020-01-14 | 深圳华大智造科技有限公司 | 一种高通量的单细胞转录组测序方法和试剂盒 |
| EP3821032A1 (en) * | 2018-07-13 | 2021-05-19 | HiFiBiO SAS | Use of droplet single cell epigenome profiling for patient stratification |
| EP3594364A1 (en) * | 2018-07-13 | 2020-01-15 | HiFiBio SAS High Fidelity Biology | Method of assaying nucleic acid in microfluidic droplets |
| US20210310063A1 (en) * | 2018-07-13 | 2021-10-07 | Hifibio Sas | Use of droplet single cell epigenome profiling for patient stratification |
| EP3730625A1 (en) * | 2019-04-24 | 2020-10-28 | HiFiBiO SAS | Use of droplet single cell epigenome profiling for patent stratification |
| EP3730626A1 (en) * | 2019-04-24 | 2020-10-28 | HiFiBiO SAS | Use of droplet single cell epigenome profiling for patient stratification |
| EP4471156A3 (en) | 2018-10-01 | 2025-02-26 | Becton, Dickinson and Company | Determining 5' transcript sequences |
| WO2020097315A1 (en) | 2018-11-08 | 2020-05-14 | Cellular Research, Inc. | Whole transcriptome analysis of single cells using random priming |
| CN113195717A (zh) | 2018-12-13 | 2021-07-30 | 贝克顿迪金森公司 | 单细胞全转录组分析中的选择性延伸 |
| WO2020154247A1 (en) | 2019-01-23 | 2020-07-30 | Cellular Research, Inc. | Oligonucleotides associated with antibodies |
| EP3924506A1 (en) | 2019-02-14 | 2021-12-22 | Becton Dickinson and Company | Hybrid targeted and whole transcriptome amplification |
| CN114051534B (zh) | 2019-07-22 | 2025-02-21 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| EP4025709A1 (en) * | 2019-09-06 | 2022-07-13 | 10X Genomics, Inc. | Systems and methods for barcoding cells and cell beads |
| EP4407041A3 (en) | 2019-11-08 | 2024-09-25 | Becton Dickinson and Company | Using random priming to obtain full-length v(d)j information for immune repertoire sequencing |
| US11649497B2 (en) | 2020-01-13 | 2023-05-16 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and RNA |
| EP4471155A3 (en) | 2020-01-29 | 2024-12-18 | Becton, Dickinson and Company | Barcoded wells for spatial mapping of single cells through sequencing |
| CN115151810A (zh) | 2020-02-25 | 2022-10-04 | 贝克顿迪金森公司 | 实现使用单细胞样品作为单色补偿对照的双特异性探针 |
| CN115605614A (zh) | 2020-05-14 | 2023-01-13 | 贝克顿迪金森公司(Us) | 用于免疫组库谱分析的引物 |
| US12157913B2 (en) | 2020-06-02 | 2024-12-03 | Becton, Dickinson And Company | Oligonucleotides and beads for 5 prime gene expression assay |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| US12391940B2 (en) | 2020-07-31 | 2025-08-19 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| US11739443B2 (en) | 2020-11-20 | 2023-08-29 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
| US12392771B2 (en) | 2020-12-15 | 2025-08-19 | Becton, Dickinson And Company | Single cell secretome analysis |
| CN112592968B (zh) * | 2020-12-27 | 2022-07-26 | 苏州科诺医学检验实验室有限公司 | 高通量测序用分子标签接头及其合成方法与应用 |
| WO2025054490A1 (en) * | 2023-09-08 | 2025-03-13 | Rasyn, Llc | Multi-omic analysis using beyond-poisson droplet loading |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5641870A (en) | 1995-04-20 | 1997-06-24 | Genentech, Inc. | Low pH hydrophobic interaction chromatography for antibody purification |
| JP2006507921A (ja) | 2002-06-28 | 2006-03-09 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | 流体分散のための方法および装置 |
| EP3023140B1 (en) | 2003-04-10 | 2019-10-09 | President and Fellows of Harvard College | Formation and control of fluidic species |
| JP2006349060A (ja) | 2005-06-16 | 2006-12-28 | Ntn Corp | ボールねじ |
| WO2008109176A2 (en) | 2007-03-07 | 2008-09-12 | President And Fellows Of Harvard College | Assays and other reactions involving droplets |
| US20140057799A1 (en) * | 2010-12-16 | 2014-02-27 | Gigagen | System and Methods for Massively Parallel Analysis of Nucleic Acids in Single Cells |
| US20150005200A1 (en) * | 2012-08-14 | 2015-01-01 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US10119134B2 (en) * | 2013-03-15 | 2018-11-06 | Abvitro Llc | Single cell bar-coding for antibody discovery |
| GB2546833B (en) * | 2013-08-28 | 2018-04-18 | Cellular Res Inc | Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels |
| US20150298091A1 (en) * | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| EP3134536B1 (en) | 2014-04-21 | 2019-12-18 | President and Fellows of Harvard College | Systems and methods for barcoding nucleic acids |
| EP3872191B1 (en) * | 2014-05-22 | 2024-11-06 | The University of British Columbia | Methods for determining lymphocyte receptor chain pairs |
| EP3161160B1 (en) | 2014-06-26 | 2021-10-13 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| WO2016207441A1 (en) * | 2015-06-26 | 2016-12-29 | European Molecular Biology Laboratory | Cell barcoding in microfluidics |
-
2016
- 2016-06-28 LT LTEP16305783.9T patent/LT3263715T/lt unknown
- 2016-06-28 EP EP20150460.2A patent/EP3686287A1/en active Pending
- 2016-06-28 EP EP16305783.9A patent/EP3263715B1/en not_active Revoked
- 2016-12-08 CN CN201680080900.9A patent/CN108603220A/zh active Pending
- 2016-12-08 CA CA3007526A patent/CA3007526A1/en active Pending
- 2016-12-08 JP JP2018530753A patent/JP7016800B2/ja active Active
- 2016-12-08 US US16/060,723 patent/US20190345537A1/en active Pending
- 2016-12-08 AU AU2016367200A patent/AU2016367200B2/en active Active
- 2016-12-08 EP EP16820177.0A patent/EP3387148A1/en not_active Ceased
- 2016-12-08 WO PCT/EP2016/080341 patent/WO2017097939A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| AU2016367200A1 (en) | 2018-07-05 |
| EP3263715A1 (en) | 2018-01-03 |
| CN108603220A (zh) | 2018-09-28 |
| US20190345537A1 (en) | 2019-11-14 |
| JP2019502377A (ja) | 2019-01-31 |
| EP3686287A1 (en) | 2020-07-29 |
| EP3387148A1 (en) | 2018-10-17 |
| AU2016367200B2 (en) | 2022-06-02 |
| CA3007526A1 (en) | 2017-06-15 |
| JP7016800B2 (ja) | 2022-02-07 |
| WO2017097939A1 (en) | 2017-06-15 |
| EP3263715B1 (en) | 2020-01-08 |
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